Document Type
Article
Publication Date
3-1-2006
Abstract
Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advances in primer design for profiling of DNA polymorphism and mRNA in higher plants, as well as new primer systems developed for animals that can be adapted for plants.
Recommended Citation
Plant Methods 2006, 2:4 doi:10.1186/1746-4811-2-4
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