Source Publication (e.g., journal title)
Journal of Chromatography Open
Document Type
Article
Publication Date
11-2022
DOI
https://doi.org/10.1016/j.jcoa.2022.100038
Recommended Citation
Sherry Cox, Lainey Harvill, Sarah Bullock, Joe Smith, Joan Bergman, Validation of a method for pantoprazole and its sulfone metabolite in goat plasma using high performance liquid chromatography, Journal of Chromatography Open, Volume 2, 2022, 100038, ISSN 2772-3917, https://doi.org/10.1016/j.jcoa.2022.100038. (https://www.sciencedirect.com/science/article/pii/S2772391722000093)
Submission Type
Post-print
Files over 3MB may be slow to open. For best results, right-click and select "save as..."
COinS
Comments
A selective method for the quantitation of pantoprazole and its metabolite in goat plasma was developed and validated. Chloroform was used for drug extraction and separation occurred on a Symmetry C18 column. A combination of sodium phosphate dibasic (0.1 M, pH 7.5) and acetonitrile were used as the mobile phase (64:36, v/v) while absorbance was measured at 290 nm. With a sample size of 0.1 mL the lower limit of quantification (LLOQ) was 0.01 µg/mL. The intra-assay variability for pantoprazole ranged from 3.4 to 10% while the metabolite ranged from 3.3% to 8.7% The inter-assay variability ranged from 2.6 to 9.7% and 3.3 to 7.5% for pantoprazole and its metabolite. The recovery was over 95% for both pantoprazole and its metabolite, pantoprazole sulfone. The method was used to quantify both pantoprazole and its metabolite and was useful for pharmacokinetic studies.