Masters Theses

Date of Award

12-2000

Degree Type

Thesis

Degree Name

Master of Science

Major

Entomology and Plant Pathology

Major Professor

Bradford B. Reddick

Committee Members

Bonnie H. Ownley, Bob Trigiano

Abstract

In this study, the basis of resistance of Capsicum annuum 'Criollo de Morelos' lines 8003 and 8004 to several potyviruses was investigated. In order to evaluate viral replication in initial sites, PAS-ELISA was carried out in whole-inoculated leaf samples. In addition, tissue print immunoblot assays were employed to determine if viral coat protein antigen could be detected in stem sections. The potyviral isolates of interest were PVY-0, PVY-1, 2, PVY-TN100, TEV-HAT, TEV-CA, TEV-V49, PepMoV-CA, and PepMoV-V1182. Line 8003, 8004 and its F1 progeny, 8004-17, exhibited broad-spectrum resistance by the lack of symptoms and failure to support viral movement into the vascular system to all isolates of interest except PepMoV-CA. The CA isolate of PepMoV was able to enter the vascular system and invade young tissues in line 8004 and 8004-17. Line 8003 was unaffected by PepMoV-CA infection. Studies involving initial inoculated leaves at 7 and 14 dpi revealed the lack of detection of PVY-0, PVY-TN100, and PVY-1, 2 coat protein antigen in initial inoculated leaves up to 14 dpi in lines 8003, 8004, and 8004-17. The TEV isolates HAT, CA, and V49, were typically not detected in initial inoculated leaves in 8003, 8004, and 8004-17 while PepMoV isolates CA and V-49 were detected up to 50 % of the time at 14 dpi in 8004 and 8004-17, with 8003 unaffected.

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