Quantitative trait loci and heritability analysis of TN93-99 x PI416937 F₆ derived soybean progeny

Author

Yamika Nit'a Stokes

Date of Award

8-2002

Degree Type

Thesis

Degree Name

Master of Science

Major

Plant, Soil and Environmental Sciences

Major Professor

Vincent R. Pantalone

Committee Members

Arnold M. Saxton, Robert N. Trigiano

Abstract

Soybean is now an essential and prominent source of vegetable protein and oil with a multitude of uses in human food and animal feeds. There are numerous industrial applications for the various components of this versatile legume. In the United States, soybean is considered a major grain crop and can be found in almost any food. Soy is also used in numerous industrial products. Most of the U.S. soybean production occurs throughout the Corn Belt to the Mississippi Delta.

One of the most important aspects of plant breeding is the improvement of the quality of crops for human and animal consumption. The use of molecular markers to detect improved traits created by crossing two cultivars is efficient due to its speediness and relative ease of manipulation. Knowing the location or loci on the chromosomes will make future crosses and trait detection easier for plant breeders.

This research intended to (1) use simple sequence repeat (SSR) markers to identify quantitative trait loci (QTL) for plant maturity, plant height, plant lodging, seed size, seed yield, seed oil concentration, and seed protein concentration in an F₆ population from a cross between the soybean lines TN93-99 and PI 416937 and (2) conduct a field test on F₆ pure lines to assess heritability for each quantitative trait.

TN93-99 and PI 416937 were polymorphic for 26% of the total simple sequence repeat markers screened. In the progeny screening for single F₆ plants grown at Knoxville Plant Science Farm in 2000, only 1% of markers displayed significant QTL for plant height [Satt409 (MLG A2) and Satt442 (MLG H)], seed size [Satt583, Satt444, and Satt430 (all on MLG Bl)], seed oil concentration [Satt373 (MLG L)], and seed protein concentration [Satt373 (MLG L)]. QTL analyses were conducted on replicated F_6:8 plots grown at Knoxville and Springfield, TN, and in a combined analysis. Several major QTL (R² >10%) and minor QTL (R²2=10%), Satt331 on MLG O (R² =16%), and Satt581 on MLG O (R²=19%). The minor QTL for plant maturity were Satt272 (R²=7%), and Satt556 (R²=7%) both on MLG B2, and Satt495 on MLG L (R² =7%). The plant height major QTL were Satt200 (R²=12%), and Satt225 (R²= 10%) on MLG Al, Sattl72 on MLG Dlb+W (R²=l 1%), and Satt581 on MLG O (R²= 12%). The three minor plant height QTL were Satt272 (R²=7%) on MLG B2, Satt367 (R²=9%) on MLG I, and Satt574 (R² =9%) on MLG J. There was no major plant lodging QTL discovered for the combined analysis. Minor plant lodging QTL were Satt442 (R² =6%) on MLG H, and Satt495 (R² =8%) on MLG L. Seed size major QTL were Satt200 (R² = 11 %) and Satt599 (R² = 16%), both on MLG Al. There were no minor QTL for seed size in the combined analysis. Seed yield major QTL were Satt545 (R²=16%) and Satt599 (R²= 20%) with both markers on MLG Al, Satt272 (R²=10%), and Satt556 (R²=12%) on MLG B2. There was a minor QTL for seed yield at Satt373 (R² =9%) on MLG L. Major QTL for seed oil concentration were Satt030 (R²=21%) and Sattl46 (R²=17%) on MLG F, and Satt215 (R²=12%) and Satt547 (R²=10%) on MLG J. A minor seed oil concentration QTL was found at Satt419 (R²=8%) on MLG I. Major QTL for seed protein concentration were Satt373 on MLG L (R² = 10%), and Satt345 on MLG O (R²= 13%). There were no minor QTL for seed protein concentration in the combined analysis.

Recommended Citation

Stokes, Yamika Nit'a, "Quantitative trait loci and heritability analysis of TN93-99 x PI416937 F₆ derived soybean progeny. " Master's Thesis, University of Tennessee, 2002.
https://trace.tennessee.edu/utk_gradthes/6595