Masters Theses

Date of Award

12-2004

Degree Type

Thesis

Degree Name

Master of Arts

Major

Anthropology

Major Professor

Lyle Konigsberg

Committee Members

Richard Jantz, Karla Matteson

Abstract

The recent proliferation of techniques suitable for DNA recovery in ancient samples has prompted anthropological researchers to explore molecular-based investigations of human remains from archaeological contexts. The study demonstrates the utility of ancient DNA analysis to strengthen a site-specific demographic profile from the Punta Lobos assemblage. DNA extraction via a silica-based extraction technique from juvenile hair bulb samples and further DNA amplification via high cycle-number PRC was undertaken to genetically type individual sex. Morphologically intermediate subadult samples were typed as biological males when amplification was successful. The inclusion of preliminary amplification results form a mitochondrial DNA marker suggests that DNA concentration in the Punta Lobos hair samples is sufficient for an extended tri-system genetic analysis.

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