Phylogenetic analysis and revision of the Nearctic Androprosopa Mik (Diptera: Thaumaleidae) with an emphasis on the western species

Author

Robert John Pivar, University of Tennessee - KnoxvilleFollow

Date of Award

12-2015

Degree Type

Thesis

Degree Name

Master of Science

Major

Entomology and Plant Pathology

Major Professor

John K. Moulton

Committee Members

Ernest Bernard, Becky Trout Fryxell

Abstract

The family Thaumaleidae, also known as seepage midges, is an uncommonly encountered, understudied family of aquatic Diptera compared to its sister group, the Simuliidae. The goal of this project was to assess species diversity of the Nearctic Thaumaleidae and determine relationships among them. The western Nearctic fauna of Androprosopa Mik is revised to include twenty-eight species, six of which are described as new to science. Descriptions of the adult males of A. apache, A. arnaudi, A. magnipelvim, A. rainierensis, A. sierra and A. uvas are provided. Redescriptions of all remaining species are also provided, as well as genitalic illustrations, updated distribution maps, and a dichotomous key to males of all western Nearctic Androprosopa. Molecular phylogenetic analyses were conducted to determine relationships between eastern and western Nearctic Androprosopa species and between the more structurally heterogeneous western species. Fresh, molecular grade material- was gathered for all Nearctic species, except seven (A. arnaudi, A. fusca, A. sonorensis, A. schmidiana, A. uvas, A. zelmae and A. zempoala ) that eluded capture. Molecular sequence data from two nuclear protein-coding genes, big zinc finger (BZF) and molybdenum cofactor sulfurase (MCS) were acquired using the polymerase chain reaction and Sanger sequencing methods. Analyses using maximum parsimony, maximum likelihood and Bayesian inference methods were conducted. Resulting phylogenies supported monophyly of the western Nearctic species; however, relationships between the western and eastern species were unresolved, as were the relationships between the eastern Nearctic, western Nearctic, and Palearctic species. Based upon both structural and molecular data, six new species groups within the western Nearctic fauna are proposed.

Recommended Citation

Pivar, Robert John, "Phylogenetic analysis and revision of the Nearctic Androprosopa Mik (Diptera: Thaumaleidae) with an emphasis on the western species. " Master's Thesis, University of Tennessee, 2015.
https://trace.tennessee.edu/utk_gradthes/3600