Masters Theses
Date of Award
6-1981
Degree Type
Thesis
Degree Name
Master of Science
Major
Chemistry
Major Professor
Earl L. Wehry
Committee Members
G. Mamantov, J. Bloor
Abstract
High performance liquid chromatography (HPLC) was employed for normal-phase separation of a complex sample (a shale oil) into fractions containing major classes of compounds. A polar bonded-phase column (Partisil-10 PAC) facilitated the separation. Gradient elution was employed, with n-heptane and dichloromethane comprising the mobile phase. The total elution time was approximately thirty minutes. Room-temperature solution fluorometry, HPLC and gas chromatography were utilized to identify the various fractions of the three-component mixture which was used as a model for the shale oil separation. The polynuclear aromatic hydrocarbon (PAH) fraction of the shale oil was analyzed by matrix-isolation fluorometry. Most of the work used nitrogen matrices. Benzo(b)fluorene, chrysene, p3rrene, benz(a)anthracene and benzo(a)pyrene were identified in the PAH fraction. A quantitative analysis revealed 27 ± 3 ppm BaP in the shale oil sample. These results compared favorably with the results reported by the National Bureau of Standards (NBS).
Recommended Citation
Mohou-Neill, Sumaria Indra, "Normal-phase high performance liquid chromatography and characterization of polycyclic aromatic hydrocarbon fraction by matrix-isolation fluorometry. " Master's Thesis, University of Tennessee, 1981.
https://trace.tennessee.edu/utk_gradthes/15256