Masters Theses

Date of Award

12-1987

Degree Type

Thesis

Degree Name

Master of Science

Major

Chemistry

Major Professor

Michael J. Sepaniak

Committee Members

George K., James Q. C.

Abstract

Rare earth metal chelate fluorescent labels for immunoassays possess extremely long fluorescence lifetimes and permit the effective use of time-resolved detection. This is shown to be very important in fiber optic-based fluorimetry, which is a technique that ordinarily exhibits large signal backgrounds due to backscatter radiation. Using time-resolved detection to reject the backscatter radiation, the fiber optic-based limit of detection for Eu-2-naphthoyltrifluoroacetone is 10-12 M; nearly three orders of magnitude lower than for the fiber optic-based measurement of the most common fluorescent label, fluorescein isothiocyanate. Commercially available Eu-chelate labeled reagents are used to demonstrate the potential utility of time-resolved fluorimetry in fiber optic-based immunoassays. Rabbit Immunoglobulin G (IgG) is covalently bonded to the distal end of quartz optical fibers prior to exposing to Eu-chelate labelled anti-rabbit IgG. The limit of detection for the assay is approximately 1 x 10-4 mg/mL.

Download
Files over 3MB may be slow to open. For best results, right-click and select "save as..."

Share

COinS