Masters Theses

Date of Award

12-1991

Degree Type

Thesis

Degree Name

Master of Science

Major

Chemical Engineering

Major Professor

George C. Frazier

Abstract

The systematic effect of hexose composition (at constant total sugars) on growth and anthocyanin accumulation was determined for wild carrot cells in batch suspension cultures. Glucose as a single carbon source gave the highest anthocyanin accumulation, while fructose as a single carbon source gave the highest growth rate, least time to maximum biomass, and the greatest amount of biomass. The anthocyanin accumulation remains relatively low and approximately constant until glucose exceeds 50% of the total sugar, after which the anthocyanins increase with increased glucose. The dry weight decreases as the glucose is increased from zero to about 50% of the total sugars and remains approximately constant as the glucose is increased to 100% of the sugar. These results suggest that the metabolism of glucose and fructose is quantitatively different in wild carrot, despite the interconversion of their 6- phosphates in the glycolysis pathway. The results for cells grown on sucrose are not significantly different from the cells grown on a 50 - 50 mixture of glucose and fructose. After lagging growth by about two days, anthocyanin accumulation increases exponentially and halts when growth ceases, suggesting that anthocyanin accumulation is growth associated. A simple model based on 15 reactions was shown to be capable of providing a reasonably good fit of the growth data and uptake data for the sugars, ammonium, phosphate, and succinate for each of the six experiments separately. However, when the average values of the kinetics parameters were used in an attempt to fit the data for cells grown on fructose, the model failed to provide an accurate fit to the data. This result suggests that additional model development work is required before a predictive cell growth model can be validated.

Download
Files over 3MB may be slow to open. For best results, right-click and select "save as..."

Share

COinS