Search for the human homolog of S. cerevisiae PTR2 gene

Author

Mohankumar Ramaswamy Sowlay

Date of Award

12-1994

Degree Type

Thesis

Degree Name

Master of Science

Major

Life Sciences

Major Professor

Jeffery M. Becker

Committee Members

Donald K. Dougall, Gary Stacey

Abstract

Peptide transport is an evolutionarily conserved phenomenon with functional significance in cell nutrition. An attempt to identify a human homolog of the S. cerevisiae peptide transport gene (PTR2) was made in order to study the molecular features of the putative human gene. S. cerevisiae ptr2 mutant PB1X-9B was transformed with a HeLa cell cDNA library and ptr2⁺ transformants were selected directly on dipeptide medium. A single clone with a 10kb insert was obtained. Subsequently, the complementing region was subcloned as a 6kb fragment of the insert. Southern and DNA sequence analyses showed that the isolated clone was identical to the S. cerevisiae PTR2 and not derived from HeLa cell DNA. The explanation for this result is not apparent.

Recommended Citation

Sowlay, Mohankumar Ramaswamy, "Search for the human homolog of S. cerevisiae PTR2 gene. " Master's Thesis, University of Tennessee, 1994.
https://trace.tennessee.edu/utk_gradthes/11696