Date of Award
Master of Science
Charles N. Stewart Jr.
Reggie Millwood, Feng Chen, Terek Hewezi
Stable transformation of soybean (Glycine max) is a markedly slow and laborious process. Thus, a tool that enables rapid evaluation of genetic elements in planta is critical to advance complex research and genetic engineering in soybean. To that end, a substantially robust agroinfiltration method was innovated in this work. Agroinfiltration is a technique that leverages Agrobacterium-mediated transient transformation to deliver genetic elements to the cells of whole plant tissues, usually leaves. Several factors were found to be relevant to successful soybean leaf agroinfiltration, including genotype, surfactant, developmental stage, and Agrobacterium culture medium. The research represents not only a new research tool for soybean biotechnology, but also indicates critical parameters for guided agroinfiltration optimization that could be used for other crop species.
Trull, Bryce N., "A robust agroinfiltration method. " Master's Thesis, University of Tennessee, 2023.