Doctoral Dissertations
Date of Award
8-2016
Degree Type
Dissertation
Degree Name
Doctor of Philosophy
Major
Life Sciences
Major Professor
Albrecht von Arnim
Committee Members
Mariano Labrador, Michael Gilchrist, Michael Langston
Abstract
Translation of mRNA into protein is a critical step in gene expression, but the principles guiding its regulation at the genome level are not completely understood. Translation can be quantified at a genome scale by measuring the ribosome loading of mRNA—the extent to which mRNA is associated with ribosomes. In this dissertation, I present investigations into how genome-wide ribosome loading is controlled in Arabidopsis thaliana. In chapter 1, I give an overview of regulation of ribosome loading and translation. In chapter 2, I present research demonstrating for the first time that genome-wide ribosome loading in plants is partially controlled by the circadian clock. In chapter 3, I present a study of a computational model that describes how various biochemical steps control ribosome loading. And in chapter 4, I conclude by briefly summarizing the dissertation as a whole and discussing future perspectives.
Recommended Citation
Ernest, Joseph Benjamin, "Computational Analyses of mRNA Ribosome Loading in Arabidopsis Thaliana. " PhD diss., University of Tennessee, 2016.
https://trace.tennessee.edu/utk_graddiss/3910