Prolactin-enhanced uteroglobin synthesis and secretion by uteri of estrogen and progesterone treated juvenile rabbits

Author

Gary W. Randall

Date of Award

12-1986

Degree Type

Dissertation

Degree Name

Doctor of Philosophy

Major

Zoology

Major Professor

Joesph C. Daniel

Committee Members

Jeff MacCabe, John Koontz

Abstract

Juvenile (22 day old) and neonatal (nine day old) female rabbits were exposed to a nine day injection protocol. Each day, the animals were either sham (carrier only) injected or received a subcutaneous injection of estradiol (either 10 or 100 micrograms/kg/24hr^-1) for the first two days, ovine prolactin (14 I.U./kg/24hr^-1) for the following three days and progesterone (3 mg/kg/24hr^-1) for the remaining four days of the nine day injection period. Eleven treatment groups existed for each age group. Juvenile animals, which received the 100 micrograms estradiol/kg/24hr^-1, sham, progesterone protocol or the 100 microgram estradiol/kg/24hr^-1, ovine prolactin, progesterone protocol, produced detectable amounts of uteroglobin (UG) in their uterine secretions (0.034 ± 0.030 mg UG and 0.098 ± 0.023 mg UG/mg total protein, respectively). Verification of de novo uterine UG synthesis was verified by ³⁵S-methionine incorporation, in vitro, into UG, at a significant level relative to total protein incorporation of radiolabel.

Recommended Citation

Randall, Gary W., "Prolactin-enhanced uteroglobin synthesis and secretion by uteri of estrogen and progesterone treated juvenile rabbits. " PhD diss., University of Tennessee, 1986.
https://trace.tennessee.edu/utk_graddiss/12456