Doctoral Dissertations

Date of Award

5-1995

Degree Type

Dissertation

Degree Name

Doctor of Philosophy

Major

Chemistry

Major Professor

Michael J. Sepaniak

Abstract

Investigations of alternatives to classical detection techniques for capillary electrokinetic separations are reported. Endeavors to convert a commercial absorbance photodiode array detector for use as a fluorometric photodiode array detector are detailed. New methods of micro-fabrication are developed and employed in the production of low dead volume junctions for two novel detectors. The application of the junction as a post capillary reactor for peroxyoxalate chemiluminescence detection is presented. Approximate detection limits (e.g. 2.5 x 10-7; M for perylene) are presented with a linear response over 3 orders of magnitude. A novel post capillary flow multiplexer is developed which improves fraction collection and off-line detection capabilities for capillary electrokinetic separation techniques. Principles involved in switching electroosmotic flow are identified and their connotations for application of the post capillary flow multiplexer reported

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