Staphylococcus ursi sp. nov., a new member of the ‘Staphylococcus intermedius group’ isolated from healthy black bears Open Access

Source Publication (e.g., journal title)

Int. J. Syst. Evol. Microbiol.

Authors

Vincent Perreten, University of Bern, Switzerland
Stephen A. Kania, University of Tennessee, Knoxville
David Bemis, University of Tennessee, Knoxville

Document Type

Article

Publication Date

2020

DOI

https://doi.org/10.1099/ijsem.0.004324

Abstract

Six Staphylococcus strains were isolated from healthy black bears (Ursus americanus) in the Great Smoky Mountains National Park, Tennessee, USA. Phylogenetic analysis based on complete genome, 16S rRNA, dnaJ, hsp60, rpoB and sodAgenes, and MALDI-TOF-MS main spectral profiles revealed that the strains belonged to one species and showed the closest relatedness to members of the ‘Staphylococcus intermedius group’ (SIG), which include Staphylococcus intermedius, Staphylococcus pseudintermedius, Staphylococcus delphini and Staphyloccoccus cornubiensis. The strains were positive in SIG-specific and negative in individual species-specific PCR assays for the nuc gene. The strains can be differentiated from the other SIG species by the absence of sucrose fermentation, from S. intermedius DSM 20373^T, S. pseudintermedius CCUG 49543^T and S. cornubiensis DSM 105366^T by the absence of methyl β-d-glucopyranoside fermentation and from S. delphini DSM 20771^T by fermentation of trehalose. DNA relatedness of the type strain MI 10-1553^T with the type strains of S. delphini, S. pseudintermedius, S. intermedius and S. cornubiensis was ≤48.2 % by digital DNA–DNA hybridization and ≤92.3 % by average nucleotide identity calculations. Iso-C_15:0, anteiso-C_15 : 0 and anteiso-^C17 : 0were the most common fatty acids. Polar lipids consisted of phosphadidylglycerols, phospholipids, glycolipid, diphosphatidylglycerol and aminophospholipid. Cell-wall peptidoglycan was of type A3α l-Lys-Gly₃ (Ser; similar to A11.2 and A11.3). The respiratory quinone belonged to menaquinone 7 (MK-7). The G+C content of MI 10-1553^T was 39.3 mol%. The isolated strains represent a novel species of the genus Staphylococcus, for which we propose the name Staphylococcus ursi sp. nov. The type strain is MI 10-1553^T (=ATCC TSD-55^T=CCOS 1900^T).

Recommended Citation

Perreten, Vincent; Kania, Stephen A.; and Bemis, David, "Staphylococcus ursi sp. nov., a new member of the ‘Staphylococcus intermedius group’ isolated from healthy black bears Open Access" (2020). Faculty Publications and Other Works -- Biomedical and Diagnostic Sciences.
https://trace.tennessee.edu/utk_compmedpubs/159