Determination of Thiafentanil in Plasma Using LC–MS

Source Publication (e.g., journal title)

Journal of Chromatographic Science

Authors

Sherry Cox, University of Tennessee, Knoxville
Joan Bergman, University of Tennessee, Knoxville
Matthew C. Allender, University of Illinois
Kimberlee Beckmen, Alaska Department of Fish and Game
William Lance, Wildlife Pharmaceuticals Inc

Document Type

Article

Publication Date

2020

DOI

10.1093/chromsci/bmz098

Abstract

A new method of analysis has been developed and validated for the determination of thiafentanil in plasma. After protein precipitation, samples were separated on an XBridge BEH C18 column and quantified using mass spectrometry. The mobile phase was a mixture of water with 0.1% formic acid and acetonitrile with 0.1% formic acid (90:10). The standard curve ranged from 0.1 to 25 ng/mL. Intra- and Inter-assay variability for thiafentanil was less than 10%, and the average recovery was greater than 95%. The lower limit of quantification was 0.1 ng/mL. This is the first validated method for thiafentanil analysis in plasma.

Recommended Citation

Cox, Sherry; Bergman, Joan; Allender, Matthew C.; Beckmen, Kimberlee; and Lance, William, "Determination of Thiafentanil in Plasma Using LC–MS" (2020). Faculty Publications and Other Works -- Biomedical and Diagnostic Sciences.
https://trace.tennessee.edu/utk_compmedpubs/151

Submission Type

Publisher's Version