A Validated Method for the Determination of Firocoxib in Equine Tissues

Source Publication (e.g., journal title)

Austin Chromatogr

Authors

S. Cox, University of Tennessee, Knoxville
J. Hayes, University of Tennessee, Knoxville
K. Gordon, University of Tennessee, Knoxville
M. White, University of Tennessee, Knoxville
M. Macpherson, Large Animal Clinical Sciences, University of Florida

Document Type

Article

Publication Date

2015

Abstract

A new reversed-phase High Performance Liquid Chromatography (HPLC) method was developed and validated for the determination of firocoxib in equine tissue. Firocoxib was detected by ultraviolet detection at 290nm after undergoing a liquid extraction using ethyl acetate: hexane (40:60) and separation on a Sunfire C₁₈ column. The mobile phase consisted of water with 0.025% trifluoroacetic acid and acetonitrile (50:50), with a flow rate 1.1 mL/min. A concentration range of 5-1500ng/gm produced a linear curve with r²>0.99. The lower limit of quantification was 5ng/gm. The intra and inter assay variability was <10% and the average recovery was 97%.

Recommended Citation

Cox, S.; Hayes, J.; Gordon, K.; White, M.; and Macpherson, M., "A Validated Method for the Determination of Firocoxib in Equine Tissues" (2015). Faculty Publications and Other Works -- Biomedical and Diagnostic Sciences.
https://trace.tennessee.edu/utk_compmedpubs/147