Masters Theses

Date of Award

8-2019

Degree Type

Thesis

Degree Name

Master of Science

Major

Food Science

Orcid ID

http://orcid.org/0000-0003-4265-1533

Major Professor

Thomas G. Denes

Committee Members

John Peter Munafo Jr., Doris D'Souza

Abstract

Listeriosis, a foodborne illness that may lead to serious illness and/or death in immunocompromised individuals, is caused by the Gram-positive bacterial pathogen Listeria monocytogenes. Gram-positive bacteria contain in their cell walls a thick layer of peptidoglycan, which attaches surface glycopolymers known as wall teichoic acids (WTA). WTA are vital for many functions in the cell, but the primary interest within these studies concerns their role as bacteriophage receptors. Bacteriophages, viruses that exclusively infect bacteria, have been used for over a decade as antimicrobial agents to control L. monocytogenes in ready-to-eat foods and food processing facilities. However, an ever present concern is the possibility of bacterial resistance developing after use of such products. Mutations that predominantly lead to phage resistance are those that affect biosynthesis or glycosylation of WTA. The objectives of these studies include analysis of the WTA monomers of three phage-resistant mutant strains of L. monocytogenes 10403S (FSL D4-0014, FSL D4-0119, and UTK P1-0001), their cross-resistance to a diverse collection of bacteriophages, and to determine if the methodology analyzing WTA monomer units could be streamlined for their rapid assessment. It was found that the mutant strain which lacked rhamnose on its WTA (FSL D4-0119) was the most resistant to phages, with only one phage able to successfully infect it. These findings can be utilized in the formulation of new bacteriophage products that are better able to prevent bacterial resistance from occurring. Using gas chromatography with flame ionization detection, it was observed that the previously uncharacterized mutant strain, UTK P1-0001, does appear to contain a small amount of the full WTA monomer found in its parent strain, which could help to explain its unique pattern of phage resistance. It was also found that this process can be streamlined as far back as using cells that have only been autoclaved to see chromatographic peaks of the WTA monomer unit. Additionally, the methods used here can be employed to develop a library of known WTA monomers for a rapid determination of their presence or absence, saving significant time and materials.

Comments

Chapter II of this document has been accepted for publication in the journal "Food Microbiology".

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