The Sugar Content of Blood

Summary and Conclusions:

1 We have shown that the picric acid methods are not accurate.

2. We have shown that the titrimetric methods are not adapted to clinical use.

3. We have demonstrated that the Folin-Wu method, which gives the same results

as the titrimetric methods, and is the most accurate method used in clinics,

gives results approximately 15 per cent too high, because of interfering

substances.

4. We have developed a mercuric nitrate method, specific for blood sugar by

virtue of the fact that it precipitates interfering substances before making the

determination.

5. We have developed an acetone method, specific for blood sugar, which gives

the same results as the mercuric nitrate method.

6. We have adapted our mercuric nitrate method and acetone method for

determining glucose in tissue extracts.