Calcium, Angiotensin Converting Enzyme Inhibitors, and Branched Chain Amino Acids Contribute to the Anti-Obesity Effects of Milk
Date of Award
Master of Science
Michael B. Zemel
Gary E. Truett, Jung Han Kim
Dietary calcium exerts an anti-obesity effect, and dairy exerts about twice the activity as supplemental calcium. Milk contains angiotensin converting enzyme inhibitors (ACEi) and a high concentration of branched chain amino acids (BCAA), both of which may contribute to an anti-obesity effect through effects on lipid metabolism and muscle protein synthesis, respectively. To test this hypothesis, aP2-agouti transgenic mice were maintained for 6 weeks on an obesigenic soy based diet and then randomized into an ad libitum control group and energy restricted (70% of ad lib) diets containing soy protein (0.4% Ca), non-fat dry milk (NFDM; 1.2% Ca) or Ca-depleted NFDM (0.4% Ca) for an additional 6 weeks; the soy diet was provided with or without an ACEi and BCAA supplement and provided at either 0.4 or 1.2% Ca. The NFDM group exhibited ~2-fold greater reduction in body weight and fat than the energy restricted group (p
Herweyer, Anna C., "Calcium, Angiotensin Converting Enzyme Inhibitors, and Branched Chain Amino Acids Contribute to the Anti-Obesity Effects of Milk. " Master's Thesis, University of Tennessee, 2007.