Date of Award
Master of Science
Walter R. Farkas
Daniel C. White, Charles C. Cogdon
The total amount of mRNA recovered from Pb2+-treated rabbits was not significantly influenced by lead but dose-dependent loss of template activity of the poly A+ fraction was observed. These observations lead to the conclusion that the messenger RNA of rabbit reticulocytes exposed to Pb2+ in vivo was damaged and inactivated without extensive depolymerization of the mRNA.
In vitro experiments on the effect of Pb2+ on purified rabbit globin mRNA were also performed. After incubation with Pb2+ at micromolar concentrations, the 7-methyl guanosine cap was removed from the mRNA, while damage to the remainder of the molecule seems to be slight. These data indicate that Pb2+ causes the loss of the template activity of reticulocyte mRNA in vivo by causing a nucleolytic cleavage of the mRNA at or near to the 5' cap.
Aethranis, C. A., "Effect of Lead on Globin mRNA In Vivo and In Vitro. " Master's Thesis, University of Tennessee, 1993.