Event Title
Sorghum Phenolic Extracts: Chemical Characterization and Biological Activity Determination
Faculty Mentor
Philipus Pangloli, Vermont P Dia
Department (e.g. History, Chemistry, Finance, etc.)
Food Science
College (e.g. College of Engineering, College of Arts & Sciences, Haslam College of Business, etc.)
College of Agricultural Sciences and Natural Resources
Year
2017
Abstract
Red sorghum contains phytochemicals such as 3-deoxyanthocyanidins and flavonoids with reported health benefits. The objective was to determine the chemical composition of sorghum phenolic extracts that were extracted with acidified methanol and separated into co-pigments and anthocyanin fraction by chromatographic procedure. In addition, the ability of sorghum extract to modify activation of the inflammasomes, a macromolecular protein complex involved in several malignancies, was evaluated. The total polyphenols, total flavonoids and total anthocyanins of the sorghum phenolic extract was quantified and phenolic profile was determined by high performance liquid chromatography. The ability to modify the inflammasomes was evaluated using THP-1 human macrophages as an in vitro model. Treatment of sorghum phenolics in lipopolysaccharide-primed and adenosine triphosphate-activated THP-1 human macrophages resulted in reduction in IL-1β and IL-18 secretion. Our study showed the potential of sorghum phenolics to serve as a chemopreventive agent against diseases associated with aberrant activation of the inflammasomes.
Included in
Sorghum Phenolic Extracts: Chemical Characterization and Biological Activity Determination
Red sorghum contains phytochemicals such as 3-deoxyanthocyanidins and flavonoids with reported health benefits. The objective was to determine the chemical composition of sorghum phenolic extracts that were extracted with acidified methanol and separated into co-pigments and anthocyanin fraction by chromatographic procedure. In addition, the ability of sorghum extract to modify activation of the inflammasomes, a macromolecular protein complex involved in several malignancies, was evaluated. The total polyphenols, total flavonoids and total anthocyanins of the sorghum phenolic extract was quantified and phenolic profile was determined by high performance liquid chromatography. The ability to modify the inflammasomes was evaluated using THP-1 human macrophages as an in vitro model. Treatment of sorghum phenolics in lipopolysaccharide-primed and adenosine triphosphate-activated THP-1 human macrophages resulted in reduction in IL-1β and IL-18 secretion. Our study showed the potential of sorghum phenolics to serve as a chemopreventive agent against diseases associated with aberrant activation of the inflammasomes.