The effects of kinetin and naphthaleneacetic acid on in vitro shoot multiplication and rooting in the fishtail fern

Beck, Michael Jerome

The effects of kinetin and naphthaleneacetic acid on in vitro shoot multiplication and rooting in the fishtail fern

Date Issued

March 1, 1980

Author(s)

Beck, Michael Jerome

Advisor(s)

James D. Caponetti

Additional Advisor(s)

Karen Hughes

John Day

Permanent URI

https://trace.tennessee.edu/handle/20.500.14382/37108

Abstract

This study is concerned with the determination of the shoot and root inducing effects of kinetin (K) and naphthalene acetic acid (NAA) on shoots of the Fishtail Fern (Nephrolepis falcata forma furcans) in sterile tissue culture. These hormones were tested in Murashige Fern Multiplication Medium at 0, 10^-7, 5x10^-7, 10^-6, 5x10^-6, 10^-5, 5x10^-5, 10^-4M concentrations alone and in combination with each other. This was done to determine the best combinations for shoot multiplication and rooting that could be applied to rapid commercial tissue culture propagation.

It was found that shoots on medium containing 10^-5MK and 5x10^-5M NAA produced the greatest mean number (8.2) of new shoots. Over a broad range of NAA concentrations, shoots on medium containing low concentrations of K (0 to 10^-6M) produced larger mean root numbers (5.8 to 12.4) than shoots on medium containing high concentrations of K (5x10^-6M to 5x10^-5m). Mean root numbers varied from as many as 6.5 to as few as 0.3. The above data can be used as a guide to rapid commercial propagation of Fishtail Fern.

Degree

Master of Science

Major

Botany

File(s)

Name

Thesis80B424.pdf

Size

1.4 MB

Format

Unknown

Checksum (MD5)

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