Parental bone marrow growth in young hybrid mice
Date Issued
August 1, 1980
Author(s)
Chervenak, Robert Paul
Advisor(s)
Charles C. C., T. T. O., John R. S.
Abstract
When bone marrow is transplanted from certain inbred mouse strains to F1 hybrids of that strain, the graft often fails to proliferate. It has been reported that this phenomenon, known as Poor Growth, is not demonstrable in recipients less than three weeks of age. The purpose of the present study was to investigate some of the parameters involved in this phenomenon and its sudden appearance at three weeks of age. By employing 125IUdR uptake and hemopoietic colony assays following transplantation of marrow to mice of various ages and treatment groups, the following conclusions were drawn. 1) Parental marrow grew equally well in both parental strain and F1 hybrid recipients less than three weeks old; 2) The observed growth of hemopoietic tissue was not due to endogeneous stem cell proliferation; 3) Changes in radiation sensitivity did not account for the fluctuations of hemopoiesis seen in mice from one to five weeks of age. 4) Factors in milk, either specific or nonspecific did not influence the outcome of parental marrow grafts; 5) Neither stimulator cells in mice less than three weeks of age nor graft destroying cells in older mice could be demonstrated; 6) Natural Killer cells were unable to effect in vitro cytalysis of bone marrow or the hemopoletic stem cell compartment thereof.
Two mechanistic models of Poor Growth are presented and discussed and a new model is proposed.
Degree
Doctor of Philosophy
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Thesis80b.C447.pdf
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4.56 MB
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