Perceval: A Genetically Encoded Fluorescent Reporter of ATP:ADP Ratio and its Potential for Application in Plant Cells

This project explored the potential of using Perceval, a genetically encoded fluorescent protein sensor, to monitor ATP:ADP ratios in plant cells. Because plant cells contain chlorophyll, which has strong natural fluorescence, a major challenge was determining whether Perceval’s signal could still be detected in that background. To begin, the Perceval plasmid was successfully purified using both commercial kits and an in-lab protocol, and its identity was confirmed through restriction enzyme digestion and DNA gel electrophoresis. Several rounds of bacterial transformation were attempted, and ultimately, Perceval was successfully introduced into E. coli cells and induced to express protein. Preliminary protein expression was observed on an SDS-PAGE gel, though confirmation of successful protein expression remains a step for future work. While this study did not reach the point of fluorescence testing in the presence of chlorophyll, it established reliable protocols and identified key troubleshooting steps for plasmid prep, transformation, and expression. The methods and progress described here provide a solid foundation for future research aimed at applying Perceval as a real-time, in vivo reporter of energy dynamics in photosynthetic systems.