TTX Blocks Baclofen-induced Phase Shifts of the Mammalian Circadian Pacemaker in Vitro

Prosser, Rebecca; Bergeron, Harriet E.; Danielson, Blaine; Biggs, Kevin R.

TTX Blocks Baclofen-induced Phase Shifts of the Mammalian Circadian Pacemaker in Vitro

Date Issued

September 1, 1999

Author(s)

Prosser, Rebecca

Bergeron, Harriet E.

Danielson, Blaine

Biggs, Kevin R.

Link to full text

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6SYR-3XMGP1C-1N&_user=422010&_coverDate=09%2F11%2F1999&_rdoc=21&_fmt=high&_orig=browse&_srch=doc-info%28%23toc%234841%231999%23991589998%23133549%23FLA%23display%23Volume%29&_cdi=4841&_sort=d&_docanchor=&_ct=23&_acct=C000019958&_version=1&_urlVersion=0&_userid=422010&md5=9e8ad4cafe52ef745ac65c338af02055

Permanent URI

https://trace.tennessee.edu/handle/20.500.14382/15613

Abstract

The mammalian circadian pacemaker, located in the suprachiasmatic nucleus (SCN), expresses 24-h rhythms when isolated in vitro. The GABA_A agonist, muscimol, induces phase advances during the mid-subjective day, while the GABA_B agonist, baclofen, induces both daytime phase advances and nighttime phase delays. Here, we present evidence that tetrodotoxin (TTX) completely blocks baclofen-induced phase shifts in vitro, but does not block in vitro phase advances induced by muscimol. These results suggest that GABA_A, but not GABA_B, receptors are located on SCN pacemaker cells.

Subjects

Circadian rhythm; Sup...

Disciplines

Biochemistry

Cell Biology

Molecular Biology

Recommended Citation

Harriet E. Bergeron, Blaine Danielson, Kevin R. Biggs, Rebecca A. Prosser, TTX blocks baclofen-induced phase shifts of the mammalian circadian pacemaker in vitro, Brain Research, Volume 841, Issues 1-2, 11 September 1999, Pages 193-196, ISSN 0006-8993, DOI: 10.1016/S0006-8993(99)01791-6. (http://www.sciencedirect.com/science/article/B6SYR-3XMGP1C-1N/2/e38cabf25c7bc2f309425678bcb83f89)

Embargo Date

June 9, 2010