Callus production and organogenesis in sweet pepper (Capsicum annuum L.)

Cotyledon and hypocotyl explants of tissue cultured seedlings of three varieties of pepper, Capsicxim annuum L., were aseptically cultured on MS medium supplemented with the auxins 2,4-D, NAA, and lAA separately in a range of concentrations from 0 to 5x10 ^-5M. Callus formation occurred on media with all three auxins in four weeks. Medium with 10^-6 to 10^-5M 2,4-D produced the greatest fresh and dry weights of callus on the explants of all three varieties. In many instances, roots formed on callus on media with NAA and lAA, but not with 2,4-D. Organogenesis of shoot buds was achieved best on cotyledon explants on medium with a combination of the auxin lAA and the cytokinin BA