>M1.Bag18758II [EnzType=Type_II_methyltransferase] [RecSeq=CACGGG]
MHLERYNPLPSLVHSPSRSASKQQALLLAPQELYEPDPEIVTAAKLKSTNTTDKARQRDLAELLRRINLSVLSQDRSDLAAFVNWKGNAESPIHRWLRYR
EAYSPNLITKLGLGNEILDPFCGCGSILIGAAENGNTAAGIDINPLAIFAAKVKLTPLSRTQLQAVQNYVDRLDSALNTARPWPMPELSIASKVFEPVIL
DTLLRIRSLIESTFSGDIECRNFLHLAWVAILERVGSYFKEGNGIKYRNKKRLKTGYTIRPEGQWQLERFGRDQYKFTLNAFCEHVRMMLNDARFWRKGA
WRSQTIIEGSVLEMDKLLPLRKFDSIVFSPPYANRFDYFESMKVELWFGGFVDSYESINRFRKASLRSHLGADLNRSYRHIEDLEQLIALMDRDASSWRM
GVPELLRGYFDDMRITLKHCRKLLVENGKCFVVVGNSAFAGVIIPTDVLLANLGLECGFKKAEILITRHLTVAPQQRNKLSNLEENMRESVVVLS
>M.Hpy99XII [EnzType=Type_II_methyltransferase] [RecSeq=GTAC]
MIRTPKHLTKQESVNLGAYYTPPYLVDCAYKLLKKHVGIENYTLLDTACGNKEFLKLHHPKKIGADIDPKCDALIINALANPKRENYGISQDEPLIIVGN
PPYNDRTSFIKQDIKNKDFIFEIDNDLKSRDLGISFLKSFAILKPAFICVLHPLSYLIKEANFKQLKLFKDHYRLLDAFVVSSKSFTKSNEFPIVIALYE
RGRMDYAGIRRFVFPTDCDTTLCLNDFDYIANYVDKYPNAKKVGACVGYFFPMRDINALKRNKTFLNAPSENAVRISQDKLIYYQYIHYFKEIAPKIPYY
FGNLDIIIDHFAFLEIKDAFLKDKRARLEYFKKLFQGHPCEFD
>PhoI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGCC]
MYKVGRYLVDSLQIYFPASLEIQEELINNGFYVPRSPDRKVSMPIPIVYSDFGGRVISIERLIPPEWLEISPEQLGWEETYLENKRGFKLPKEEVYVDVS
ISNDSIIFELDVKNYHLERTSIRGINPEKWKNWVMFYIDLKYVDEFINALREHIPAFENKTRVIREKQQGGKEVTYYAKVNVKNFSLCLGCFDLAQRYLQ
IKAKEHCNIYPGSPTCNDSLSKLKLRLEYDPSITTFAKVGIAKISGKRPQIMVKLTSTETKTIRGILKPEIKGKARGKLVYCDHREKRQYIALDLFDFYK
ALVSTKKYEGKLPTDD
>Afa22MI [EnzType=Type_II_restriction_enzyme] [RecSeq=CGATCG]
MRPNRYLQLIEEIFRARWKKGTTEFDFARTDLEAVAADLGIELPKNLGDVMYALRYRIGFPESILETQPAGQEWIIEGAGRARYRFRLVKKTRILPREDL
ARIAVPDATPELIRAYALDDEQALLAIVRYNRLIDTFLGLTTYSLQNHLRTTVKGIGQIEIDELYVGLDKRGCHYIIPVQAKGGTDQIGVVQTTQDIRFV
EQKYPGMRCRAVSAQFMEEGVVALFELTLQDDEVRVVEERHYKLVPHTELDPKAIRDYR
>S.LhoZ1I [EnzType=Type_I_specificity_subunit] [RecSeq=GCCNNNNNCTCC]
MKNEFLKPGWRRVRFGDVVRLSKARSQDPLADGIERYVGLEHLEPGDLRIRSWGSVADGVTFTSVFQPGQVLFGKRRAYQRKVAVADFSGVCSGDIYVLE
TKDSLVLLPELLPFICQTDAFFDHAVGTSAGSLSPRTNWTSLADFEFVLPPIDEQQSAIALLSAATDQCHAIEAAHRAAGRMLQSFKDSLLLNKTSSLAN
SYLLGDLLLRSPESGCSAPPKDADTGYFVLGLAALSRDGYVSGDFKPVEPTSKMVAAKLSMGDMLISRSNTVERVGFVGIFSDNRDDVSFPDTMMRLQPN
PALVHPDFLEALLQTTSAREFLMRIAAGTSASMKKINRANLLQMRLNVPDLDAQELALDELQKFKNAIATQKARWDAARQLTRLIAMRTIGGAA
>M.BssHI [EnzType=Type_II_methyltransferase] [RecSeq=CTCGAG]
MDRKRWQANVRSMEIVAKPPEAITDEDIAFLRENYTSMGGLLPKGFNGGAFFTPTHVARFMTEAVAGLCGGFAPGLRVLEPSVGSGVFLEHLPPDAEVTA
LELDETSAKVTSLIYPRANVILGNALEHGRRDYYDLVIGNPPYGESVELADGSDWITLSKSRGSYRGKSENAFLELAIKAAKPGGYIAYVLPLGIGYSQA
SAKVRKL
>Sse9I [EnzType=Type_II_restriction_enzyme] [RecSeq=AATT]
MGMNSRLLSQRARQPGHDQTFRESESNFVEALEMILDPDEWRVEDHPPELRRIIGGRYGVVPEASIEYLPTGRKFFFEVKKQGPAGNADERACKHHTVQF
YKELHALFGYDYHPFATIMCESLATLERYTVKHPFYFEEGHYFCWVDYDVDLLADFIAQIASRWLMDPTAEPPQALPQ
>CatHI [EnzType=Type_II_restriction_enzyme] [RecSeq=CTCTTC]
MAKQKKYGFRTPIRRTTKNITGNKVGGVLTGSEAEIKYSKKIIIDKTLTIHYKIPKELAVSLFNSNIGIAALGGYFLSSKDIKILFSLNVSGNESKIEYN
LSANRYESIGHDLEVDLDEDFSVINASIVFECSERVSVNYTHFGIGFVNKDAYIESEEAYRHYSNSKKRICFPEQFYFDNYVEFDNSSEGSIILTKSCNR
CQRFLPINPFNQRQQLAFSNHCTTKAPCTHKGFSIYNIVSNNISEDSLISFQKKLLDKGYSFEDGNLISYFGHQLECKACKKFFVNAALNHLRSSSQHRE
DSLRRRAFELLSCRLLDRKWIYHEFRKNTGKEFDKYIFDKFEKSCFKCGVAIKSSKKMHLDHTMPLSHLYPLDESATCLCASCNLAKSDMFPIDFYTENE
LERLSVLTSLPLELLKSRSPNELVITELKNKILWFIDDFLNHPEYIKLRDGKKAADSILHSVNKAVSKSSNPFNIINIYNEAKG
>AatII [EnzType=Type_II_restriction_enzyme] [RecSeq=GACGTC]
MNPDEVFSDFQRGFFGRKFTAGLLVSFIDLMSELETPKLGIADFDGFLKLFPRQLKTSAGKRANTLIVEKEDGKTISLRKFYNSIEKFYRAEHKRFDYPS
AAPHATQAWADYKTWLDALVTFSEEQLGELRGRVNQFVLDTLKSQEFDPTSVKVEPPLFRILLEKFEMTAQKGEPTGASFQGIVFGFLRADNPHLQIEID
KVRTGSKRLQRIGDVDGWEGERLAISAEVKQYEINTESIDDLADFANRTGQRGALGVIAALSFSEEAKPLLENMGLIALDKEGMLKIVELWDPVKQRTAV
SSFIYYATHVEKNSSLSARLNIFLEASASEWAEQRQAAILPQSES
>M.HpyGI [EnzType=Type_II_methyltransferase] [RecSeq=TCNNGA]
MQTLFKEITPKRYVNGNEMKENSSNVLDQYFTKPSVALKCFQKACEVIKKYENPDDFIFLEPSAGDGVFYDLFPKNRRIGIDIEPKRDGFIQCDFLNYKL
PTHQKVICLGNPPFGHRGVMALEFINHARNCDFVCFILPMFFESQGKGSIKYRVKGLNLLYSERLEKNAFIDFKNKEVDVHCVFQIWSKKYQNKKSEFSW
YKNRHKEPFGEYIKVFTVSLAKNRECGKEWIFNQKASFYISSTFYKSTQIVENFEEVKYQSGIAVVFTSTNKALNAKLKKLFKEIDWTKYASLATNSCYH
LGKSHIFQALYDHLDSLKDN
>S.EcoR15I [EnzType=Type_I_specificity_subunit] 
WEWTTLTRIAEINPKIDVSDDEQEISFIPMPLISTKFDGSHEFEIKKWKDVKKGYTHFANGDIAIAKITPCFENSKAAIFSGLKNGIGVGTTELHVARPF
SDIINRKYLLLNFKSPNFLKSGESQMTGSAGQKRVPRFFFEN
>Eco933II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAABCC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>EcoKMcrBC_(EcoKMcrC) [EnzType=Type_IV_methyl-directed_restriction_enzyme] 
MEQPVIPVRNIYYMLTYAWGYLQEIKQANLEAIPGNNLLDILGYVLNKGVLQLSRRGLELDYNPNTEIIPGIKGRIEFAKTIRGFHLNHGKTVSTFDMLN
EDTLANRIIKSTLAILIKHEKLNSTIRDEARSLYRKLPGISTLHLTPQHFSYLNGGKNTRYYKFVISVCKFIVNNSIPGQNKGHYRFYDFERNEKEMSLL
YQKFLYEFCRRELTSANTTRSYLKWDASSISDQSLNLLPRMETDITIRSSEKILIVDAKYYKSIFSRRMGTEKFHSQNLYQLMNYLWSLKPENGENIGGL
LIYPHVDTAVKHRYKINGFDIGLCTVNLGQEWPCIHQELLDIFDEYLK
>M.PfrJS7aV [EnzType=Type_II_methyltransferase] [RecSeq=CTGAAG]
MRTRDEVSDDKLRGGFYSPDVLVDLCIKRSVELLGGRTGIRVLEPAAGDGAFLRGIKRSPLAGLVERLEAVEIIDHEAHKSAVALDDLGVSGEVFNQNIL
RWSQGREANFDLVLANPPYVRFQFISDADKQLAKTISAELGTAGSSVSNLWIPVFLLSIAKLRVGGVFSIILPTEFLTGISAGAVRNWLLDNTTDLTIDL
FKPGSFPSVLQEVLILTGRAAAHVADSTATISFFDHNGGTRSWKHSVRRGTGTWTNYLLTPDQNSAMEAARSIIGIQPLSRVARFSVSTVTGANAYFCVD
DQIVEDYHLREWAMPLLPRSRNAEGLVFAKKEHQDLVNTGQVAWLLSFSADKPSPESLLRPRKFLARGVAQQIDQRFKCRVRTPWYRVPVVPTGALLLSK
RSNRFPRVIANHAEVVTTDTIYRGQIVAGATVTADAFTASFHNSLTMLSAEIEGRSFGGGVLELVPSEVNRLLVPVVPGAEAELPRLDKIARTSRDPEAL
IEATDAMLARILPALDGSTLADLGDARRTLMDRRLQRTHSKFYG
>OspHL35III [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=YAGGAG]
MNAVEIEEAISRLAEQPFDAEEFPFAFLEAFGNKATTIKRLRSGASNKSDLGGVLQINNIHIKVCAEGQVSDTIRALRESPATKNTRNRVKFIMATDGVT
LEAEDLSSDIAPIVCPFEDFPDHFGFFLPLAGISTVKQIRESAFDIKATGRLNRLYVELLKDNPEWGTAERRHDMNHFMARLIFCFFAEDTDIFVKADQF
TATIEQMSAPDSTNTHEVISTLFRAMNTKAEERDAANTPRWASDFPYVNGGLFSESVDVPRFSKIARSYLLHIGNLDWKKINPDIFGSMIQAVADDEERG
ALGMHYTSVPNILKVLNPLFLDDLREKLEEAGENARKLLNLRNRMARIRVFDPACGSGNFLVIAYKEMRAIEAEINKRRGEPDRRTDIPLTNFRGIELRD
FSAEVARLALIIAEYQCDVLYRGQREALRDFLPLDAMNWITCGNALRIDWLSICPPTGTGVKHHAEDLFHSPLDQAQIDFENEGGETYICGNPPYLGSQG
QSGEQKADLSSVFSGTNANWKGFDYVAGWFKKAADFLETVDARFAFVSTNSICQGKAVSNLWPVLLQNDLEIFFANQNFKWTNLAAHNAGVIVSIIGVRR
VSSQPKYLSSINPDGTEEIRKVSNINAYLTGSQDIYVEAASKPIGEKAKMVFGNMPIDGGHLSFSMSELNALNLETETRRRLIKRLLGGAEFVRGQQRFC
LWLNDETVKEARNFPELKRRLDKVRGFRMASKRSATADLADTPYRFGWIAQTGREIVTLLPRILSERREYIAVGTEPAGTLTTDQAYVLLDAPLWNMALL
ASRLHIVWIATVCGKLKTDLRYSNTLGWNTFPIPKLTEKNKEDLTHCAEDILLAREAHFPATIADLYDPEKMPADLRAAHDRNDEVLERIYLGRRFRNDT
ERLEKLFELYTKMTAAKDLKKTNSRMKA
>Pal408I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CCRTGAG]
MTADIETFITRWQSTGGSERANYQLFLTELCAALDLPQPDPAQDDTRDNAYVFERRVTFRHGDGSSSQGFIDLYRRGCFIGEAKKVKKNEGRAFDDALLR
ARSQAENYARALPASEGRPPFLMVVDVGHVIELYAEFSQSGATYTPFPDPRSHRIRLSDLRQPEIQQRLRQLWLEPLELNPARRTAAVTRQIAAQLALIA
RALEADQHPPQAVAGFLTRCIFTFFAEDVGLLPQRSFTELLETLSQAPEQFVPLVADLWQTMDRGGFSVLLRKELLRFNGKLFKSPDVLPLKRAQIDLLI
EAGKADWTQVEPAIIGTLLERALEPRERHKLGAHYTPRAYVERLVLPTVIEPLREDWQLAQATALTLAKEGDTKNALKALRQFHQHLCNVRVLDPACGSG
NFLYVTLEHLKRLEGEVLNQMESLGASQSFETEGLTVDPHQFLGLEVNPRAAALAELVLWIGYLQWHFRTRKDATPPLPVLKDFRTIECRDAVLDYDSVD
FVVDEHGKPVTRWDGRSFKKHPVTGEDVPDEAAQTPQERYLNPRKAVWPEAEFVVGNPPFIGASRMRDALGDGYTEALRNTWPHVPESSDFVMYWWHHAA
ELTRAGALRQFGLITTNSIRQTFNRRVIEQHLNPVIPAKAGIQAARAARRTATEETTEKLDSRLRGNDGAALVLTFAVPDHPWVDSGDGAAVRIAMTVGA
APGADAPPSPPTPLPRAGEGGRRLGEGAATGRVLRVLKETGEREAEGMRAEFASQCGAINADLSVGADVAGAKQLISSKGVSVRGLVLMGAGFVVNESQA
RELGLSRAGHESIIRPFRNGRDLTERPRNVYVIDAFGLSENVLRSQFPAIFQWLHSRVKPERDLNPRKGRRENWWLFGEPNPRLRESQAGLQRYIATVMT
AKHRIFLFVDREIAADQGLVSIALDDGASHGTLSSRVHAAWALASGGRLGMGNDSRYNNSRCFETFPFPNLNPDQSTRIGKLAEAIDRHRKQQQAAHPNL
TLTGCYNVLEALRQQRPLTAKEKVIHEQGLVSVLASLHDDLDREVLAAYGWGDLAPDLVGKPGGTTPFAEPDDAQAAAQAELLNRLVALNTERAAEEARG
HIRWLRPEFQNPQGGTANTQIEADMDVEDDSPAPVVAPIKRQPWPPELPAQVRSVADVLTAARVPLSLEAIAACFTGKGPWKKRLPQILDTLEALGRIKP
EGAGYRAVN
>M.Maf25III [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGSWDGV
AGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEV
VLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGELADVDVGIVTGRNSFFTFTDAKAQA
LGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAPRLTV
NAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDG
LGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>Awo1030IV [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GCCRAG]
MTDVEQRAAAKQFAEIWKDQGYEKGQSQPFWLSLLRDIYGVKNPEQFIIFEDQVVLDHTSFIDGIIPETHVLIEQKGINKDLRKAIKQSDGTMLSPFQQA
KRYSADLPYSKRPRWIVTCNFKAFLIYDMEKPNGEPEEILLENLPAETYRLEFLVDTGDSHIKKEMEISIQAGELVGELYDELLKQYHQPDDPESLKCLN
MLCVRLVFCLYAEDAGIFGKHGKFHSYLKDYDAKDVRRALIDLFAVLDTQPADRDPYLEPTLASFPYVNGGLFSEKNIEIPNFNDTIVNLLLQNASEDFD
WSAISPTIFGAVFESTLNPATRRSGGMHYTSIENIHKVIDPLFLDELKEEFADIKAIAIEKTRNRRLDDFQTKLSRLTFLDPACGSGNFLTESYLALRKL
ENEVLKVLLGDQIRIGTDTHTPIKVSINQFYGIEINDFAVSVGKTALWIAESQMMMKTEDIIHMHLDFLPLKTYANIIEGNALKLDWETVVAKTSLDYIM
GNPPFVGYSMQNDGQKKDILAVYLDEQGKSYKTAGKIDFVAGWYFKAAQLMDQTKIRTAFVSTNSITQGEQVAGVWKPLYERFGIHIDFAYQTFKWASEA
KDKAAVHCVIVGFSQAYNAKEKYLYADVMINQVTTINPYLVEAPVVFIENIKKPICNVPEMTTGNRPADGGHLLISDKDYDLFIEKEPKSKNYIKQFVGA
AEFINNKKRWCLWLVNASPTEIRSMPLVKERVELCRIDRLNAPDAGRQKLAERPALFRETKNPKSYVSVPSTSSENRRYIPIGFLDDEYIPSNSTIILPD
ATIYDFGILTSNVHMAWMRTVAGRLKSDYRYSINIVYNNFIWPTPTDKQQDMIEKTAQEILNARALFPDSSFADLYDETVMPSALRKAHQLNDRAVMDAY
GFDKKISESECVAKLMRLYQEKVTAVENKITK
>M.CfrAI [EnzType=Type_I_methyltransferase] [RecSeq=GCANNNNNNNNGTGG]
MDEVIARNFNLDIKNPHQGGNRQATNPDELLAQYAKQQEAIQTLRHQLRDILGAALSGKEVN
>EcoKMcrA [EnzType=Type_IV_methyl-directed_restriction_enzyme] [RecSeq=YCGR]
MHVFDNNGIELKAECSIGEEDGVYGLILESWGPGDRNKDYNIALDYIIERLVDSGVSQVVVYLASSSVRKHMHSLDERKIHPGEYFTLIGNSPRDIRLKM
CGYQAYFSRTGRKEIPSGNRTKRILINVPGIYSDSFWASIIRGELSELSQPTDDESLLNMRVSKLIKKTLSQPEGSRKPVEVERLQKVYVRDPMVKAWIL
QQSKGICENCGKNAPFYLNDGNPYLEVHHVIPLSSGGADTTDNCVALCPNCHRELHYSKNAKELIEMLYVNINRLQK
>S.XorL8I [EnzType=Type_I_specificity_subunit] [RecSeq=GAGNNNNNNCCT]
MAEHFHTLFTTESSIDALKQTLLQLAVMGKLVPQDPNDEPASELLKRIAEEKARLVVEGKINKQKPLAAFDEEAAPFELPAGWKWTSLAQVAVVNPRNAA
ADSLEISFVPMTFIGTRFDGQHAQETRLWAKVKQGFTHFAEGDVGVAKITPCFENSKACVFSNLVNGLGAGTTELHVVRPVCQTLDPRYVLAYLKSPQFL
LVGETKMTGTAGQKRLPKNFVETNPFPLPPLAEQHRIVAKVDELMALCDQLKARLGEARQVHGNLANALIGQALNGEKEPRAEAVYFSAYLISKLASRRT
FGRVAHMKLLFLADSHLGLGLMDGYRRHAAGPLDTSIYRVEERAAKEGLYSTSIEVLKSGQEKVSYHIGANISRAVETAASALGSARKELDRLIALFEGR
KTDDLEAVATLYAVWNDALAGGLHPSDEWLINEFRSNWHEAKERFTPDVLGRWLGWMRDNGLVPTGNSATTKSQAAFLFN
>MmeI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=TCCRAC]
MALSWNEIRRKAIEFSKRWEDASDENSQAKPFLIDFFEVFGITNKRVATFEHAVKKFAKAHKEQSRGFVDLFWPGILLIEMKSRGKDLDKAYDQALDYFS
GIAERDLPRYVLVCDFQRFRLTDLITKESVEFLLKDLYQNVRSFGFIAGYQTQVIKPQDPINIKAAERMGKLHDTLKLVGYEGHALELYLVRLLFCLFAE
DTTIFEKSLFQEYIETKTLEDGSDLAHHINTLFYVLNTPEQKRLKNLDEHLAAFPYINGKLFEEPLPPAQFDKAMREALLDLCSLDWSRISPAIFGSLFQ
SIMDAKKRRNLGAHYTSEANILKLIKPLFLDELWVEFEKVKNNKNKLLAFHKKLRGLTFFDPACGCGNFLVITYRELRLLEIEVLRGLHRGGQQVLDIEH
LIQINVDQFFGIEIEEFPAQIAQVALWLTDHQMNMKISDEFGNYFARIPLKSTPHILNANALQIDWNDVLEAKKCCFILGNPPFVGKSKQTPGQKADLLS
VFGNLKSASDLDLVAAWYPKAAHYIQTNANIRCAFVSTNSITQGEQVSLLWPLLLSLGIKINFAHRTFSWTNEASGVAAVHCVIIGFGLKDSDEKIIYEY
ESINGEPLAIKAKNINPYLRDGVDVIACKRQQPISKLPSMRYGNKPTDDGNFLFTDEEKNQFITNEPSSEKYFRRFVGGDEFINNTSRWCLWLDGADISE
IRAMPLVLARIKKVQEFRLKSSAKPTRQSASTPMKFFYISQPDTDYLLIPETSSENRQFIPIGFVDRNVISSNATYHIPSAEPLIFGLLSSTMHNCWMRN
VGGRLESRYRYSASLVYNTFPWIQPNEKQSKAIEEAAFAILKARSNYPNESLAGLYDPKTMPSELLKAHQKLDKAVDSVYGFKGPNTEIARIAFLFETYQ
KMTSLLPPEKEIKKSKGKN
>M.UbaMSORFD12 [EnzType=Type_II_methyltransferase] 
MKGHVAETLKEGALFEDEDKPVVGNTYHRDGVSLYHGDALRLYADWDAPVAIISDGAYGLGLFPGDPPTADLLPAWYEPHIRAWAARATPQTTLWFWNTE
IGWATVHPLLQQHGWKYVNCHIWDKGLAHIAGNSNTQVVRKFPVVTEVCVQYVREVRIDGLNLQDWLRREWERTGLSLAVANEACGVRNAATRKYLTRDH
MWYFPPPEVFERLAQFANTHGRPEGRPYFSLDGIRPATREEWARLRPKFTCKVGSQMYGACHRSTGVNEYE
>M.Hpy57II [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MVSNTTLQKNLDAFYTHPKIARFCLDLLKDLIHQNLGLGLNAFHFLEPSAGSGSFVGALKGLGIADCLALDIAPKAQGIQKKDYLLELIEFNKKRIIIGN
PPFGHRGKLALDFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIAPPKIRHNDFI
TYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALMIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>Hpy87AI [EnzType=Type_II_restriction_enzyme] [RecSeq=GANTC]
MALYLPKGLLMDYQTFNEIFNRFVFGTSKAKLLENIAENPERYLGIFRPTKPKTKLLQNLLTSHEIKFGDAFECLIEQYLKEHNFSPLSKKIPYYNKDKE
KRESLELDQFAKKDNTYYFIEQKMRDDHDSTKKRGQIDNFERKLEALVHRYGENIQGYFYFIDEGLNKNQNYYKEELQKLSVDYGVPLSLCYGKGLFESL
NIPQVWDEVLSHLVRWRETLPDLPSLNFDENPLESFREIKDLAPSVYRKLLDNDEIFNLVLILFPEQKVLKMLVEHFRQQN
>Eco1130II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAABCC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>Hin4II [EnzType=Type_II_restriction_enzyme] [RecSeq=CCTTC]
MNLDYIKSFLSRHNYDVRKSGDARWIDQKCTYDVLSVVSDCILEYIENTNNKEFTVKDIWLSEYARENVISLFSKPDPKLKASNEYDKYFGQPIKLLSYS
KILKYRKSGNKFFYMVDELELLKYIALRPSNSLEFLISYINKVLSDSDIIENLISFYQNQDNASYKDLKDRFCNFTIQYTNINKMTECGRIFTKIINPLA
FKMKKKGTEKGRLSKDIITLLDISYNRENWRDELSGKSKTETRSDFERKDFIIKNNWDKYNANKSKKFLKYFNDREYNGKSEVNQKTEMVLATQAHHIFP
RMEYPEIATFLENLIMLTPNQHFSMAHPKNNTQYIDKNFQYICLIAKIGKIMENLTQDKIEKIYSFDNCKHVLNIGIKTTEFSNIRENDFYSILEKIDFF
YSDFSVYEDLKTANYPVA
>BspNCI [EnzType=Type_II_restriction_enzyme] [RecSeq=CCAGA]
MKMNVLGNSYTNVSTAQANIVDSFVAPSNKIGGGAGEARLYVSSQRSSVHNGTFFNFNPSNRIGKYVAAEGLCFFEKDNLIKYLEDAKVYYEMPIANYRE
NISQYYSERFATVTSLDDINTFSIYNQSGSLDSDRFYIGSKDPLWELMRIISLPKITTLYIHKMVNDVDSSDIKYYFELFITKKKDSRYLPSITKETVKI
EETIKNDTSIDSTEKETLIKARKGQGKFRRNTLSIMPNCPFTNISEPTLLRSSHIKPWAHCLTNEQRLDGNNGLTLTPTYDVLFDKGLISFEDDGRLLVS
ENLKADIIQALNLVPNQIYDISNSTHSKSVYLQYHRKHIFN
>AquII [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GCCGNAC]
MTDFIQKWQNSEGNERANYQSFLNDFCEFLGVEKSPPKGSGNNSYCFDRDVKIIAPSGAETTNFIDFYKEDCFVLETKQGSNTSNKGHGKRGTAAYRKEM
KKAFGQALKYARFVEPKPPFLITCDIGDHFRVWQDFSESWLSANGNYGTYDSVPKIPFTDLEKPEIQDFFYKVFTDPQSLNPEKIAAQVTREVAADLAEL
AKTLEQTTKPQEVAQFLMRCIFTMFAEDVGLLKEHLFTEALKERWIPQPQDFKPQVEALWQAMNDGTSFGFHGQLLRFNGGLFAKPQAIALTADQLKILL
TAAERDWKNVEPAIFGTLLERALEKKERSKLGAHYTPRAYVERLVRPVIIEPLQEKWQLIQGEVETLLEEEEAAKSASAKTKKRNAAAEKLTEFLGELRK
IRVLDPACGSGNFLYVTMDLMKTLELEVLNRLGTVMGASQLRLDFDQINPSQFLGIEINPRAAEIADLVIWIGYLQWHFRLFGSLPPVEPVLREYKNIEN
RDAVLDYDGTKPAIDPKTGKVRTRWGGRTMKHPVTGEDVPDPSDQVEILEYINPREAQWQQADYIVSNPPFLGNARMREYLGDGYTETLRKVYKDVPDTV
DFVMYWWHKAAELIRKEKTLRFGFITTNSIRQARLRSVIDFHFNQKKRIRLFFAIPDHPWSDGEVAVRISMTGVELKKKRRQYSQLTHILEESKLNTPEE
TAFSLKFSYVKANEIFSNLQFGYDVNQANSLSSNQNLASQGFVVGGSGFVLKNQALVENLEQEIIHPFKTGRDLTQSPEFRQTIDVNHLSKKQLLSSYPK
TYQWLSETVKLERSTNNDPKLKREWWRYRRANTSIRDGIKDLNRYIATVRTAKHRVFQFLNSEIMAESGVVMIFLDDSYFLGICSSSLHIIWALAQGGRL
EDRPVYNHDACFYRFPFPDPSEELKQEIRELGERLDSHRKQVQAAHPEVTITAMYNCLEKMRSGEPFTDGDREFNNKALITTLKQIHDDLDQAVFCAYGW
EDLIPLWQKVSLPKGDLEGCQTEPNNTETKEQLEQSILQRLVDLNAERAEEERNGFVRWLRPEYQAPDQVVTQKVIEGIGVEEETKEAVIAPPEQQKFPT
KLKGQLAAIRDLLRTQGGEWTITQIAAQFKGTSAKKLETIQNCLEILEDLGVILSHTETETKCYYATL
>Sth20745III [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GGACGAC]
MTPQQFIAKWAHASLKERAAAQQHFIDLCLLLGQPTPAQADAQGAWYTFEKGVTKAGGGDGWADVWMRGHFAWEYKSPGEDLAAAYKQLVLYAADLENPP
LLVVCDIARYEIHTNFTGTKKQVYRFTNADLADPEVLGILRKVFTDPEALRPEITTESVTQEAAAKFASLAGSLTARGFAPERVARFLTRLLFCLFAEDI
GLLPKGLFTRLLDVTRQRPDTFARQILILFETMRDGGIFGVEEIARFNGDLFADPDAIELTREELGLLAEAARLDWGSIEPAIFGTLFERGLDPAKRAQL
GAHYTSRDDILRVVEPVVMEPLRREWAAVRARADVLAEAFWAAKGPSQRERRRKELASVLLAFQERLASVTILDPACGSGNFLYVALEQLKNLEKEVISY
AAHRGLSMLLPQVTPRQLHGIETNAYAHELAQIVVWIGYIQWMTMNGFQVNRDPVLEPMDTVLLMDAILDRSDPAQPREPAWPDAEFIIGNPPFLGGKRL
RTELGDEYVDAMFAVYNGRVPREADLCCYWFEKARAMIAAGRVKRAGLLATNSIRGGANRRVLERIKESGDIFMAWDDEPWVLDGAAVRISMVGFDDGTE
KTRTLDGVPVAAINPDLTGALDLTTARPLAENLNIAFMGDTKGGPFDIPGDLARQMLAAPLNPNGRPNSDVIVPWVNGLDITRRPRDMWIIDFGPDMPLE
QAALYEQPFEYVKKHVYPVRQKSRSTRNEWWLHERPRVDMRDALRGLSRFIGTPTVAKHRLFVWLSAPTLPDHQLIVFARDDDYFFGVLHSRAHELWSLR
MGTSLEDRPRYTPTTCFETFPFPRPTEEQREAIAAAARALHEHRERWLNPEGASEAELKQRTLTNLYNARPTWLDNLHRELDRAVFAAYGWPDDLSDDEI
LARLLALNLERAGA
>M.Hpy66VI [EnzType=Type_II_methyltransferase] [RecSeq=TCNNGA]
MQTLFKEVTPKRYVNGNEMKENSSNVLDQYFTKPSVALKCFQKACEIIKKYENLDDFIFLEPSAGDGVFYDLFPKNRRIGIDIEPKRDGFIQCDFLNYKL
PTHQKVICLGNPPFGHRGVMALEFINHARNCDFVCFILPMFFESQGKGSIKYRVKGLNLLYSERLEKNAFIDFKNKEVDVHCVFQIWSKKYQNKKSEFSW
YKNRHKEPFGEYIKVFTVSLAKNRECGKEWIFNQKASFYLSSTFYKSTQIVESFEEVKYKSGIAVVFTSANKALNAKLKKLFKEIDWTKYASLATNSCYH
LGKSHIFQALYDHLDSLKDN
>M.HpyHII [EnzType=Type_II_methyltransferase] [RecSeq=GTAC]
MNLGAYYTPPYLVDCAYKLLKKHVGIEDYTLLDTACGNKEFLKLQHPKKIGADIDPKCDALIINALANPKRENYGISQDEPLIIVGNPPYNDRTSFIKQD
IKNKDFIFEIDNDLKSRDLGISFLKSFAILKPAFICVLHPLSYLIKEANFKQLKLFKDHYRLLDALVVSSKSFTKSNEFPIVIALYERGRMDYAGIRRFV
FPTDCDTTLCLNDFDYIANYVDKYPNAKKVGACVGYFFPMRDINALKRNKTFLNAPSENAVRISQDKLIYYQYIHYFKEIAPKIPYYFGNLDIIIDHFAF
LKIKDAFLKDKRARLEYFKKLFQGHPCEFD
>M.HpyPIV [EnzType=Type_II_methyltransferase] [RecSeq=GTAC]
MNLGAYYTPPYLVDCAYRLLKKHVGIENYTLLDTACGNKEFLKLHHPKKIGADIDPKCGALMINALVNPKRENYGISQDEPLIIVGNPPYNDRTSFIKQD
IKNKDFIFEIDNDLKSRDLGISFLKSFAILKPAFICVLHPLSYLIKEANFKQLKLFKDHYRLLDALVVSSKSFTKSNEFPIVIALYERGRMDYAGIRRFV
FPTDCDTTLCLNDFDYIANYVDKYPNAKKVGACVGYFFPMRDINALKRNKTFLNAPSANAVYISQDKLIYYQYIHYFKEIAPKIPYYFGNLDIIIDHFAF
LEIKDAFLKDKRVRLEYFKKLFQGHPCEFD
>Tsp509I [EnzType=Type_II_restriction_enzyme] [RecSeq=AATT]
MNEMYEIAKGVASFEGAPTLPGRTTGEARGGREFEAVVAEGLLKYGRLLVTAVPSLRLRPVAAEGTSRQNHLADALAVVNEENKRVLVFRLPAFRHNPLF
AEITSGALQNDFVRVPDSFLKREFVVEEWYTPKLGELAERGWIPEEDEPYPFSGTNYPELYRRKRTQFDGVIIFLESGTLREKALLEIKSLKSSEGARVD
GNAHERFAYQNLDYLEIGALYPRTTLLLLTNDAILKYRNKYHTGIGVHALRLSYAFCWYKFEMVSSVRQYLRLFSLWKEWLEGK
>M.Bhy27164IV [EnzType=Type_II_methyltransferase] [RecSeq=CCWGG]
MNKKSIINHLETDKKLISILKKLPNNYWDFKYENTKEYTHSMHSYPAVMVAPISRNIINIVKQVMEVDSLLDPFSGSGTVLVEGMLANIKNVYGNDINPL
AIFISKVKTNKLNINKLKKEASILLEQINDDYNENINFYELADEYFEKSIDLTSKDGWGNNAPEYLKKYIDSQKNNFEIPNFKNIGYWFKPRAILQLQMI
KNNILKIEDKNIRNFLFVAFSETIRLVSNRRNGEFKMFRMKPIKVKSFRPNVLKEFTDILDKNIEKMNSFTDACNKNGITSKVKIFNNNVIDLFDIPDSS
VDLVVTSPPYGDSRTTVAYGEYSRLSLQWIDLSGLSEKEIMMIDKTLMGGTKFRKGFEFSIPSKTLKKSLEAIKNNSLERAGDVYSFYLDLEKAISSISK
KTKTGGYQFWVVGNRTVKGELLKTDKIIKEIASQYDLCYVYTIDRNIINKVMPSLNSPTNTVGKKSETMCNEHIVILRKK
>M.PciI [EnzType=Type_II_methyltransferase] [RecSeq=ACATGT]
MTNFSHSALTSYDLLGHEIVQDSEAVSSGPYLVSYDPIPVRRSTFLAGLSENVHSWFRLTPSFGPDLVRTIIKQMNLAPHSHIHDPFSGAGTTAIEASLE
GYEASCVEVNPFLYFVGKTSIDWSINADDAAAQLESIKNKYYSMSATATLDNIADLGIDIPKIHNIHRWWRNDVLKDILVLKSSIRSCTQDKYCSFFELA
LAAVLVPDLTNVTLGKLQLHFVNKDDKEINVWPTYESHAKKMIHDLSLINKQNFEFLPKIIYGDSTQKSTFSEVAGIDAIITSPPYPNRYSYIWNTRPHL
YILDMISEAKEASQIDRRTIGGTWGTATSELGKGIFSPINAVVKDALEGVHERIAGSDQLMANYVTHYFNRLFLHIEAIKPSLNPKAKLAYVVGNSWIKG
EYVATDVILAKIIEGALPGSSIDGLHRFRRRNSGKNLFETIVYSTLPV
>PciI [EnzType=Type_II_restriction_enzyme] [RecSeq=ACATGT]
MKQFADPFERRFLDAIEHHLDGISEKIKKDFTHKNFLKELNGLKGDKVYHDLGFDTAEYTLVRLIGRMSISVGRRLGEIYDKVPRYVAAARFGLQPNQIA
EVFDGLELDIALRNSLLSDDDKIHIKKITEKMSGETYSGIGIEIRYNFNPNDSSRLRKDVDVASKLSAAGLFPVYLIFSSLSPRNDAIARLKRGGWSFKQ
GQEALDFLTELLGVDIGSVLSDPIIAAETREKTSKIMKSIFESEAFQSVIPGEWSKL
>Bce14579I_(Bce14579IA) [EnzType=Type_II_restriction_enzyme] [RecSeq=GCWGC]
MYFSATLFNKYYKLLGTNDEGKLNLERTSSLAYFLALDQMQKDEEEPNAVINMYKDSLSRKNFMEKVGEIFVIAKNGSFEYQANILGCINKVEGGGNSMS
SKTGKNFLSTQVISASSSPNPVGYPKRPAASFILELGFEIEGHKHGIKKSDSWKNNLMKFIEFRKCGTDTFPLIVFLLRDKQLNNNLDFNNAIREGLGSA
YTQAVVDYLIVNAQVPSLVWDDLQNFATAPFTIDELDTELFMQDQPETNNLQPTVQVKEFKEIEIPNDISRNRIVYGAPGTGKSYKLNKDAKEFFPDDDL
YIRVTLYPNYSYSQFVGTYKPTPIYKDVEGEFYKSDMVTSIGHGKEPLIDYTFVPGPFLIQLVNALKYPKSNFVIIIEEINRANAAAVFGDVFQLLDRKD
DGESEYTITFNADVTNYLRKEGIFDLQIKIPKNLYIWATMNSADQGVTPLDTAFKRRWSFEYMPIDAGEGAVSSLEMELKFLENPILWNKFRKILNQKLK
ELMVPEDKLIGPFFMKSYELSNPESFKNKLLLYLRDDVLRHNATKFFKHQTFSDIVTAYDQGGNIFRTISKEELVRSE
>M.PfrJS7V [EnzType=Type_II_methyltransferase] [RecSeq=CTGAAG]
MRTRDEVSDDKLRGGFYSPDVLVDLCIKRSVELLGGRTGIRVLEPAAGDGAFLRGIKRSPLAGLVERLEAVEIIDHEAHKSAVALDDLGVSGEVFNQNIL
RWSQGREANFDLVLANPPYVRFQFISDADKQLAKTISAELGTAGSSVSNLWIPVFLLSIAKLRVGGVFSIILPTEFLTGISAGAVRNWLLDNTTDLTIDL
FKPGSFPSVLQEVLILTGRAAAHVADSTATISFFDHNGGTRSWKHSVRRGTGTWTNYLLTPDQNSAMEAARSIIGIQPLSRVARFSVSTVTGANAYFCVD
DQIVEDYHLREWAMPLLPRSRNAEGLVFAKKEHQDLVNTGQVAWLLSFSADKPSPESLLRPRKFLARGVAQQIDQRFKCRVRTPWYRVPVVPTGALLLSK
RSNRFPRVIANHAEVVTTDTIYRGQIVAGATVTADAFTASFHNSLTMLSAEIEGRSFGGGVLELVPSEVNRLLVPVVPGAEAELPRLDKIARTSRDPEAL
IEATDAMLARILPALDGSTLADLGDARRTLMDRRLQRTHSKFYG
>M.AgsI_(M.AgsIA) [EnzType=Type_II_methyltransferase] [RecSeq=TTSAA]
MKFDVILTNPPFQDSVNRKKTPHKLWIDFTLTVFDRLLVDGGSLVQVSPASISSPSNVVLSLMEENQTNVLRLETGHHFPQIGSTFCDYWIKKQENDPTP
TTIIKGAERFDIELSSAMTYLPNDIGRLSLSVHHKVMFSGRPSLNVEWDYVTCHNIRRRDDPPSLVEQPSPAHPYPVFHTNNLTWWSSIRQDWADQPKVM
WTRSGYTKPFYDSGVYGGTDMVYFVRVDDEAAGLALAANMNSVLMRYIYRTAKWSGFGNERVFAGLPDLPRDRALSDEEMFARFALTNEEVDHVRTALEP
RRARA
>M.Mtu10134I [EnzType=Type_II_methyltransferase] 
MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRD
FASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFL
LSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGEL
ADVDVGIVTGRNSFFTFTDAKAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWW
STPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDV
DLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>MspA1I [EnzType=Type_II_restriction_enzyme] [RecSeq=CMGCKG]
MNQELIDFCELYNLPLEHLGATLKDPKVIPMIRGKAFEFSVKDRLSQVLNQNIWHVSKPFVNPQLGSHDQDVLIKHLPTNTEITIECKLSAKGQYKFQTN
ESIFKIKCMRSRTLGPELVRRLAPLRGMSEESLSVHNDQYLVGDFDLVITSLANAFYSTNEDGIFVWDPSALGQSFLEQKYGVGLSEKQYQDAAFNDMYV
ARASDLIISETNEVLCTRKKCSNNQNCGFIPNYPLLKFNHNNLTNPSNRWVHISNIENLLLNFIEG
>SdeAII [EnzType=Type_II_restriction_enzyme] [RecSeq=CCNGG]
MGIEKLKIQKNQFDINLKFYFILKYLLKAGLNEKDIKKIIEIYSLNKGLFFKNALLSSMNDLYGTSKSGLKKKDFLTDILDLFETDLDSELFIFGLKLIA
IKPLLLQEAKTRDLLDTSKLPTLNPLSLAYDKITLFSPYSTRVSGALLSLIFFDKLENQDTVFISSDTTEFLKELSKDAIALKKIGVEPNQIFMLMFSES
INQSIISDSGSNYEDRILSVLMVIGIDKDKILKIHDKDDISTEFDFFFELDGKTYGIGAKRTLRERYKQFIKTAQMTKIDIMIEITLGIDLTEEKVKAIR
NHNVFLFISDEIYSSNKYLQNISGVYSSKDLTLQTLKTLL
>Pdu1735I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CACCAC]
MNPVEIEAAVSELAIQPFDKEEFPFAFLRAFGNKETTIKRLRSGETNKSDLGGVLQTNNIHIVVAQPGEVTMALAELKESRATIRAKARYILATDGVDFE
AEDLESGETVACAYRGFPDHFGFFLSLAGITTVKQVRDSSFDIQATGRLNRLYVELLKDNPQWEVTDRRHEMNHFMARLIFCFFAEDTGIFNSTRLFTDT
IEQMSAQDASNTHEVISEIFRAMNTKDLDHDRVNIPRWANAFPYVNGGLFSGNVDVPRFSKIARSYLLHIGRLDWTKINPDIFGSMIQAVADDEERSELG
MHYTSVPNILKVLNPLFLDDLRVQLEVAGDNPRMLLNLRKRLSRIRVFDPACGSGNFLVIAYKQMREIENIINERRGEPGRESDIPLTNFRGIELRDFSA
EIARLALIIAEYQCNVLYRGPTLALADFLPLSSENWITCGNALRLDWLSICPPTGTDVKFHDDDLFSTPLNQAQIDFENEGGETYICGNPPYRGSQWQTD
EQKDDLQEIFSDRTRNWKSLDYVSGWLMKAADYGTRTPTVSAFVATNSICQGRQVETLWSLIFASGHQITFAHTSFKWANLASKNAGVTVVIVGISTNPG
TERRIFSEKPDGGIIVKSTGHINAYLVPGPNIIVGPRTTRISEVAEMTFGNMPNDGGGLLLAPEEAENAVRLHGVPSKFIRPFLGSQEYIRGQERRCIWV
DDSDYDEAVANEWLAARFESVRDKRQKSNRPTTQALANSPHRFGEVRQHGDERIIVVPGVSSEGRPYLPVGYLPAGSIVSNKNFAIMDAPLWNLALIASR
MHWVWIGAVCVRLEMRFSYSNTLGWNTFPIPTLTEKNKADLTRCAEEILLARETHFPATIAELYEPENMPSNLREAHEYNDEVLERIYIGRRFRNDTERL
EKLFELYTKMTTSAPQTKRSKKGANQ
>Eco9234II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAABCC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>BfiI [EnzType=Type_II_restriction_enzyme] [RecSeq=ACTGGG]
MNFFSLHPNVYATGRPKGLIGMLENVWVSNHTPGEGTLYLISGFSNYNGGVRFYETFTEHINQGGRVIAILGGSTSQRLSSRQVVEELLNRGVEVHIINR
KRILHAKLYGTSNNLGESLVVSSGNFTGPGMSQNIEASLLLDNNTTQSMGFSWNDMISEMLNQNWHIHNMTNATDASPGWNLLYDERTTNLTLDETERVT
LIVTLGHADTARIQAAPGTTAGQGTQYFWLSKDSYDFFPPLTIRNRRGTKATYSSLINMNYIDINYTDTQCRVTFEAENNFDFRLGTGKLRYTGVAKSND
IAAITRVGDSDYELRIIKQGTPEHSQLDPYAVSFIGNRGKRFGYISNEEFGRIIGVTF
>M.CagV [EnzType=Type_II_methyltransferase] [RecSeq=TTCGAA]
MRDQQQRKAEFGDFQTPLSLAREVCSLIARTGFRPASILEPTCGTGSFLKASLETFPDVSRVLGFEINPQYVLQAQYAVTRAFPHASIEVHQSDFFLTSW
SEIVKALPEPILVIGNPPWVTNAALSTWGSSNVPMKSNLDNLPGIDALTGKSNFDISEWMLRKNIEWLNGKNVILAMLCKTTVARKVLLYAWQNGVRIES
ASLYTIDAQEYFRASVDACLLFIRSNPTGNSKECQVFRSLHAQQPDSSFGLQDGMLVADVKSYLKRKDLTGTGFRGWRSGIKHDCSKVFELRIERGNLVN
GLGEFVEIEPEVLFPLLKSSDLAAHRKPHRWMLVPQRAMSDDPSRLRLDVPKAWNYLTAHAHLLDERKSSIYRNRPRFSVFGVGPYSFAPWKIAISGLYK
KLEFVQVPPFLGSPVVLDDTCYFFPCQSEEECNLLYELVTSEPAREFWSALIFWDAKRPITAQLLNSLDLMVLARLLGKECDIARTLAERQIVEYTEGAF
QRLLFREETADYDGEPVTNEFDLLTNQHMHTGKQ
>Cac824I [EnzType=Type_II_restriction_enzyme] [RecSeq=GCNGC]
MANIIWDKFSEDEKNEYIEFLKIFGSLSGLFKDNKEGANSRKPYLYYRNHEQLFSRVFNVEDLTRKDSAFDALAPWKGERIGIGLKTWIHTKDCTYQKVA
EFNKLAPEVIEPLIKNGTPEEVIYKISELRNERIMLDKRLYRTDMDVYHFITRDDDVMNIIETSYDLIDIKSIELIKSDGKTYSFKDKLHKYKFYKSKSV
LLEEFDASQGEIITKIPIQQFDDPFELIRMIKIPEQL
>S.Xci163811I [EnzType=Type_I_specificity_subunit] [RecSeq=ACCNNNNNNACT]
MTPALYHKAFKSSGAPWLGNVPTHWVVKPLWSMYRQKKITGYPEETLLSVYRDHGVIEKSSRDDNKNRASEDLSGYQLVVDGDLVTNKMKTWQGSIAVSS
LRGIVSPAYYVYTKLHDGNNAYLHHLLRSVPYITGYQSISKGIRVGQWDLEADKFRLFPVLIPPRPEQDAIVAHLDRATTRIDALVAKKTHFIELLREKR
QAMITHAVTKGLDRGAPMKDSGVEWLGEVPVTWDTAPLKSFLQLRRDIVGTASANTRLLSLTLQGVIERDLENPTGKMPASFDGYQRISAGEMVFCLFDM
DETPRTVGVAQQDGMLTGAYTVFRPQSDLWARYLYYFFLHVDEYKRLKPFYKGLRKTIRPGPFLSIQVPRPRDGEAEAIVAHLDRATSRIDTLIAKTERS
IELLREHRTALITAAVTGKIDLRPAA
>Kpn2I [EnzType=Type_II_restriction_enzyme] [RecSeq=TCCGGA]
MDILKEKIDVASRLYNLNLDHIPATLQVIEHAMLLLKNNAGYGYFGSFNGKNTQEYHSFTFNGEYSRPVRDDLFITDYDFFVSGFREFNESLRDIGSKWS
SFDSRRANKIIYTSVMSVACCFDLWKSGSRKTPGTFFEIFMAAVLKWMIPDEIFSKHIPLIDQLESDDESIDPSSVSTDIVIKSKYKNASVVIPLKITTR
ERIVQPFAQQRILDSYFGNGVYFSFLACISETQQDKKKKKVNHICVPGTIRLYQKYLSSLSGMYYCDIPERYLERDLTDIIPVRTMGDFLFDIYSFFRSQ
G
>M.SspP37I [EnzType=Type_II_methyltransferase] [RecSeq=TTCGAA]
MNNSLKVWEQIVQSYETSDVSLLNEQMRDAYGISNFFKDSEEVRFWKESYKDGELQVGIREFGDFQTPEDLADRVCKVLLKKGISPAVLIEPTFGKGNFI
RAALQNFEEIDRVYGVEIQKQYEIYFKKFILENNPVKFLGRDKLTMEIHQDDFFTHTFPEHLLEGEGEVLVLGNPPWITVAELSTLSSANMPEKSNFKKY
SGLDAITGKSNFDLSEYIITQLLTLLNKTKQGTLAMLCKNSIAKNIVHSLSNQEWNITTLELYNFDAKKEFDVATDASLFLVRLGEKGKVRVNTCDVYSL
SDPDARMKSFGWVNQKFVSDVESYELSAAIDNVSPLVWRSGVKHDCSKIMELTVTEEGKLLNGFKEYADIEETDVYNLLKSSDLKKETIPNKTRKKVIIT
QRKIGQDTLYIKDESPNLWKYLQDNRGYLDKRKSSIYKGKPDFSIFGIGDYSFAPYKVAIAGMYKKSNFSLVMPINNKPVMLDDTCYFLSFTKYLDAVFT
LLIVNSPIVQAFLQSLVFIDTQRPYTKDTLMRIDLIEVSSQLTLDDLLDYAKGFDIHIPFGVSEEDYADYKQRLAENPDTFFTGEADSANIQEG
>M.HpyG272V [EnzType=Type_II_methyltransferase] [RecSeq=GTAC]
MNLGAYYTPPYLVDCAYKLLKKHVGIEDYTLLDTACGNKEFLKLHHPKKIGADIDPNCGALVINALANPKRENYGINQDEPLIIVGNPPYNDRTSFIKQD
IKNKDFIFEIDNDLKSRDLGISFLRSFAILKPNFICVLHPLSYLIKEANFKQLKLLKDNYRLLDALVVSSKHFTKNNEFPIVIALYERGRMDYADIRRFI
FPTDCDTTLCLNDFDYIANYVDKYPNAKKVDACVGYFFPMRDINALKRNKTFLNVPSENAVRISQDKLIYYQYIHYFKEIAPKIPYYFGNLDIIIENTAF
LEIKDAFLKDKRVRLEYFKKLFQGHACEFD
>M.HindVII [EnzType=Type_II_methyltransferase] [RecSeq=BA]
MSEYLEYQNAIEGKTMANKKTFKQAPLPFIGQKRMFLKHVEIVLNKHIDGEGEGWTIVDVFGGSGLLSHTAKQLKPKATVIYNDFDGYAERLNHIDDINR
LRQIIFNCLHGIIPKNGRLSKEIKEEIINKINDFKGYKDLNCLASWLLFSGQQVGSVEALFAKDFWNCVRQSDYPTAEGYLDGIEVISESFHKLIPRYQN
QDKVLLLLDPPYLCTRQESYKQATYFDLIDFLRLINLTKPPYIFFSSTKSEFIRYLNYMQESKTDNWRAFENYKRIVVKASASKDGIYEDNMIYKF
>S.PfrJSII [EnzType=Type_I_specificity_subunit] [RecSeq=AAGNNNNNNACT]
MFRFAQYSSQRDTGIPWCPKVPSHWDTPRNKTVMVWRAEPVGAFSGEYQLLSLTKLGVVTRDLSTGKGKIPASFENYQRIRSGDMVMCLFDVDETPRTVG
RARQDGMITGAYERFVATGDTLAEYLNWFYIAMDNTKALRPLYRGLRKTIPVEEFLASHVPTPPLNEQAAIVKYLAHANARINKAIAAKRRLIAFLEEQK
VAVVNSVIRADWPRIPIKRALRSVTAGSWGAAVEESNDPVKWCVRVADFDYSSGGVADSPKTYRAISDREFSRRSLRRGDLLLEGSGGGEKTPVGRVVLF
DYDDDAVCSNFLQRLRSSDDVESEFLMLMLRRVHMSGEVRRYIKQTTGIQNLDLHAYLTHRIPMPSVLEQRRVVSDIQSEFREVDTVVSRIHHEIGLLQE
FRVRLVADVVTGQVDVRAVAATLPDFAEAAADVAEEDDPMLGEELAEVVAGDDE
>ScoA3McrA [EnzType=Type_IV_methyl-directed_restriction_enzyme] 
MAPSEITRAGILQAIAEHDRIGPEAFRATYGFHAATSYFLEHEGRLYDSKAIAGVAHMYDFGVALKPSSPGLSGGLKHAVAWLRREGFTIREAPKTFHRR
VGDVRPARRAMGPALHRPVLLLWAIGQAVARAPRLQPWSTTRDAVAPLMEKYGQVEDGVDGVRYPFWALVRDDLWCVEQAEELTLTSRGRRPTLESLNAV
DPSAGLREDDYNLLRSQPEAAASAAAGLIARYFHLLPAGLLEDFGLHELLAGRWPDALRPLLGETFKDRDAIWRAYGGQKMAGIGCLADGILSAFSDDKG
PYADGRIPDTTWIAYVGDGLSGDQKLTDGNELMAEHQAVGRALRYWHKPFQGQWSFETWAVIVQRRLRWGLGEDKLPRREFLWVLAPVPSPERETWPPEV
LEALEADTGELHDDTGDYRPSDLALTPGAPDGTESDDEAYRRLAQKAEANAERRGQLKKPTVADKYVRDPSARGAVLKRCQKRCENPECAGHPTELTKAG
LPILQVDHVNDLAKGGPDVPWNMIALCPNCHALKTYGANKVRLQRLLAATARRLHEEKLQ
>Eco3384II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAABCC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>BspEI [EnzType=Type_II_restriction_enzyme] [RecSeq=TCCGGA]
MIETVLEKVTNKNNFVTLQNYTDFALYFLEYIQKNKQATIVSQNEHVYNFYQYNSEANYQVTRPFNSKILYSHQDFLDNLGEFNKILKDLKSDRNHAKIL
DRSIINRTIYTVQQTIGFALDGLDANRTNVARKLNGDYFEQLILLLLREIGAPANNGVVKVPVNMEDKQLFNMSYQHDLILKDKKGEVKLIGSVKTTSKD
RIGKIFVDKFLYSKLTETTVPHIAIFLHDVQRKRNKDPQKFGINGTFLAGHFKGYTVKLNPLDGVYYFDPRPQMQTDVLLSEHIQTFDHLLCDDIWSYVD
>M.Csp68KII [EnzType=Type_II_methyltransferase] [RecSeq=TTCGAA]
MKKQSKNKIEYGDFQTPLILTDKICNKLIELNVCPDIIIEPTCGVGNFLKSSSHLFKDSKEIIGIEINKNYVNQFDNNFLGNTNTVRIINHDFFEIDLSK
LITNKNDNILIMGNFPWVTNSELGRIQGLNLPKKNNFQKNNGLEAITGKSNFDISEWMLIKSVDFLQNHQGYLAMLCKTSVARKIISYLSKQKYNFSYCS
TYGIDTKRYFNANVEACLLFCQFVPGREKHSFNIFDNLDTSKYTTIAYFDNRLIRDGETFEKLSYLYTNKPKIKWRSGVKHDCSKIMELEKIGNIFINGL
GEEVKIENDYIYPLVKGSNVANSNIKKNTYVLITQKSIGEDTNQIKELAPKTWNYLQSHSSYLDKRASRIYKKNPQFSIFGIGEYSFYPWKIAICGLYKK
LDFKLFHLIQNKPVMFDDTVYFISFKEENEATKMLDILNSPKIIDFYSSLIFWDEKRPIKSSILNCLNWHKI
>BtsI_(BtsIB) [EnzType=Type_II_restriction_enzyme] [RecSeq=GCAGTG]
MQIEQLMKSLTIYFDDIQEGLWFKNLHPLLESASLEAITGSLKRNPNLADVLKYDRPDIILTLNQTPILVIERTIEVPSGHNVGQRYGRLAAASEAGVPL
VYFGPYAARKHGGATEGPRYMNLRLFYALDVMQKVNGSAITTINWPVDQNFEILQDPSKDKRMKEYLEMFFDNLLKYGIAGINLAIRNSSFQAEQLAERE
KFVETMITNPEQYDVPPDSVQILNAERFFNELGISENKRIICDEVVLYQVGMTYVRSDPYTGMALLYKYLYILGSERNRCLILKFPNITTDMWKKVAFGS
RERKDVRIYRSVSDGILFADGYLSKEEL
>Gba708II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=ATGCAC]
MGSMNAVEIEEAISVLAERPFDPTEFPYAFLQAFGNKETTLSRLRAGNTNKSDVGGVLQRNNIHLAVAPPGEVSAKLSQLRESPATTKQKAKFILATDGE
TLEAEDLASGEPLACAWDELPDHFGFFLPLAGITTVRQIRENAFDIRATSKLNKLYVALLKENPAWGSDERRPDMNHFMARLIFSFFAEDTGIFAESRLF
TDTVSRMSAADSSNTHEVIGTLFRAMNTKHADRENAGIPRWAASFPYVNGGLFSDKVDVPRFSRTARSYLLQVGSLDWKQINPDIFGSMIQAVADDEERG
ALGMHYTSVPNILKVLNPLFLDDLRAKLEEAGDNSRTLLNLRKRIARIRVFDPACGSGNFLVIAYKEMRAIEHEINRRRGQPGRPSEIPPTNFRGIELRG
FAAEIARLALIIAEFQCDELYRGQKDAIQEFLPLDAENWITCGNALRLDWLAICPRTGTEVKLLGDDLFNTPLDQPEIDFENAGGETYICGNPPYKGRNK
KDSTQKGDLAAVAGPRTSKHRTMDYVAGWLLKLADYISAVPGTAGAFVATNSVCQGEQVPVIWPLVYERGVEIFFAHLSFTWSNLASHNAGVTVVVIGLA
ANGTRSKCLYSGTSMRLVASIGPYLVPNTTVIVKATTTPLGPQKKMYLGSMAKDGGNLVMSLKAARDLVQNHGVNSAFVRQFFGSEEVIYGKPRACLWID
DSHADSALKNAAIAEIVERVRRFRRQSDAPSTRAFASTPHRFVQLSAASGTRAIIVPRVSSENRPYLPVSYFAAGPVIGDKCYAIYDAPLWNMALVASRL
HWVWVGTVCVRLEMRFSYSNTLGWNTFPVPALTDQNMADLTRCAEDILLAREVHYPATIADLYDLETMPDNLREAHERNDEVLERIYIGRRFRNDTERLE
RLFAMYTQMTANEKRSSTARRMRG
>AciI [EnzType=Type_II_restriction_enzyme] [RecSeq=CCGC]
MNEHIKGSNSHGNSNELELVYAFDGKKVKDLNTNLKNFVQFIANDNNIKINNDTKLFAKYVSNNKLKQDFIVSFNERDFYISLKMGSGNSVHQEPIEDFI
KYLNTNYEVTEKICNDLRFFIWADGTLDGKGKFENRFDARYFKKNYPEKRRELLQFFEKNKVELIKHFMFVGKHNSRVDYLYHGTTSNGAWMSTKQIIDY
NIQNQIDTNKGNSPTLSVGRMSIQAWNVAKSGSESAEKKRGEIQVKYGKLKEDFKEVLKLNSSNKGTLFGDHEEFDISGTLNKNKNHFYWKMIARDLNLN
QEELNNLYVVRVSSKVMSSLSKKKVLPKSDAYIIRADLSKSFLLSKEYKLSEDDLVGIIYKKVGRSGISVKRADSKKYTIVKLTVASFEKCFEIEPEIKK
IIAGLLLYSKEKDMYKNLEILNKIGISELELINYTNRFIVDKVISCNDPKNVDIIRSTMQERTRTLIEKNLEIKKALFMGEGWYEEPYCINYIFKDGKLS
NDVFSEYIITTGSGRSKGNYTIALKPK
>Xca85IV [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=TACGAG]
MNAVEIEQAITDLAEQPFDGAEFPYAFLEAFGNKATTIKRLRAGASNKSDLGGVLQTSNIHILTCDAGRVTQTLAALKASPATAKAKAKFILATDGADFE
AEDLTSGETVACAFKDFPDHFGFFLPLAGISTVRQISENAFDIRATSRLNRLYVELLKDNPEWGTAERRHDMNNLMARLIFCFFAEDTDIFVGKGRFTET
VAQMSAKDSSNTHEVIATLFRAMNTKREDRAAAKVPRWAEDFPYVNGQLFSGGDEVPRFSKIARSYLLHVGGLDWTKINPDIFGSMIQAVAEDEERGELG
MHYTSVPNILKVLNPLFLDDLRSKLEEAGDNPRTLLNLRKRIAKIRVFDPACGSGNFLVIAYKEMRAIEAEINKRRGEPDRASGIPLTNFRGIELRDFPA
EIARLALVIAEYQCDVLYRGQKLALAEFLPLRNENWITCGNALRLDWLSICPPTGTGVKVQADDLFDSPLDQAEIDFENEGGETYICGNPPYLGSRDQKE
EQKADLRLLFDKRVENWKSLDYVAGWWIKAADYCTHTEAIAAFVSTNSICQGLQVPVLWPAIFASGCQIDFAYTSFRWANLASHNAGVTVAIVGITTQPR
SPRRLFSLDSSGSTIERQGSHINAYLALGETVIVEKASQPLSALSEMTFGNTPIDGGLLLLSRDERDALQLTSEQQRRFIRRIVGSAEFIRGLERYCLWI
EDQHLAEAQAILPLAQRIRGVRDLRLNGGKTARDIAERSHQFQRMFIGKNSTLVVPSVSSESREYLPCGYEPAGTVVSNLAFALYDAPLWNMALIASRLH
LVWIASVCGKMKTDFRYSNTLGWNTFPVPTLTEKNKADLTRCAEEILLAREHHFPATIADLYAPDGMPADLRTAHDRNDEVLERIYIGRRFKNDTERLEK
LFDLYTKMTASVAPAKGKKRKVGANA
>M.Mbo30III [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGSWDGV
AGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEV
VLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGELADVDVGIVTGRNSFFTFTDAKAQA
LGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAPRLTV
NAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDG
LGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>BanLI [EnzType=Type_II_restriction_enzyme] [RecSeq=RTCAGG]
MQITENRIKVRDVYENYENDDEEGVVGYDDRLDIRPRYQREFVYKDAKRDAVIDTVLQGLPLNVMYWAKTGDDTYEVLDGQQRTISLMEYVDGNFSIKYE
NNAMNFDNLPEDVQERILDYELLVYICDGTDSEKLKWFKTINIAGERLTNQELRNAVYAGPWVSDAKRYFSKTNCPAARYGNSKGCNGYVKGESIRQEIL
ETAIEWIADRDGTTVDGYMSAHQNDPTAQELWSYFRTVIEWTESLFPVYRREMKGLPWGLWYNQYHEQKYDPKALEDRVSELMADDDVTRKSGVYEYLLT
GHERVLNIRAFTDRDRRAAYEKQKGICPLCQQHFELSEMQADHNIPWSKGGKTILENCVMLCVKDNIKKSNK
>Ecl35734I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAAYTC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIEQENAARGAERAAREQGKEVEAGYRAPWNQLNSLATEYFRKLTDHEKQRQIPQRLADQRTRWQPLL
KALGYELNPHIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKRDSLRKKANGEYRSWQDLLSTVVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATSVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMRYLINN
ELAYYTGKRAINPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVRDGYSGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPGKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASDDLYEVKK
AGEEFNELETGYFVSKDEIGKYHDDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPITDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPAELTMNIPREDKQIFHFLLPDSGMANYSDKTVKQRYPDDFKALDSWRKEFTKSFAPDEIVDVQRISGKVEALWNTFR
QQLKAERQKTADNYPVWPAENTAQVRSSLSSKDETFRGRLEDNSTYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEMETLLDGIVVTERVTEAAEQA
TGDLFTDEGMVREESSLFSGAGRLKTDVLFRHLPRLAIVDALKKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLAMLRADTFNAIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVSGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NQHMLFPIGHRVKFSINIYTASQNQFVGFINMANLFSAKTIELCLAHDGSGEVGGIKDDETGSWNEAGHQSRILTVGMERLALFATLYDEEGTPALEARL
PALHAEPLVSVLEKFAKQPKRLGDLQGEYFSTVMFDETYAQADGTTKRHTQFPEDTSQWILSGPHFYVGTPLYKTPREICTEKGHYDCLDLLTLPDDYLP
RTNYIPACDVQEYAKRTPRVTWTESGEDEPRKVTNYYRLALRAMISIGAEKGLVPALVPKDVAHTNGVRTYCFNSSDLLIRVSGAAYSLIYDFMMKLSGR
TNLHQSIEEYALPDFVETAHHLSARVMSLSALTTSYSDFWQSSYSPDFNTQRWSRNLPQLPQDFFANLTPEWQRNCALRSDYSRRQALVEIDVLVAQALG
LTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLQTGTVSVTFDDYTRS
DEGERRTVTWQAPFIKPDREDDYKVAWSFFSEQFD
>S.EcoR70I [EnzType=Type_I_specificity_subunit] 
KYNKRFFYHALPGYLSAINANTSSVTVKHLSSRTLQDTLLPLP
>M.Hpy30VI [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MVSNTTLQKNLDAFYTHPKIARFCLDLLKDLINQNLGLDLNAFHFLEPSAGSGSFVGALKGLGIADCLALDIAPKAQGIQKKDYLLELIEFNKKRVVIGN
PPFGHRGKLALDFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIAPPKIRHNDFI
TYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALMIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>XorKI [EnzType=Type_II_restriction_enzyme] [RecSeq=CGATCG]
MNDTTKAPSRYHQLIEKIFFDGYSEGITEIPFERKALKEAAQELDIALPDNLGDVIYSIRFRTPMPAKVLATQPVGREWIIELVGRAKYRFRLATANRIV
PNPNLAVIRIPDNTPEIIGAYALDDEQALLAKVRYNRLIDIFLGLTTFSLQNHLRTSVKGVGQIEIDELYVGLDKYGCHYVIPVQAKGGSDQISAVQTAQ
DTAWCTQKYPTLRCRAISAQFVSSDQIALFELKMDDDELKVVEERHYKLVPAGELDRKEIVDYRS
>M.XfaTI [EnzType=Type_II_methyltransferase] [RecSeq=TTCGAA]
MNEAKKRKIEFGDFQTPSALARLVCERLSLLGFAPDVVIEPTCGVGAFVLTAAQQFPRAEVHGYEINPAYLDELRQSVEGLGVTRRVNLHQQDFFETDWL
AELEAMPGNVLVLGNPPWVTNAGQGAIGGTNLPQKSNFLKHKGFDAITGKANFDISEWMLIDLMRTLGARGGDLAMLVKTTVARKIIAHAERMQLGVSYA
TIFGIDAKRHFDASVDACLLAMRFTGALSDAGIDYDVHLSLDGNGTTRRVGHRLGLTVGDLVRFEQHADLIGSSPQKWRSGVKHDASSVMEFDSSDGLLR
NGLDEEVRIELEYLYPLMKGSDIGSNRDWRGKFVLVTQRKPEDETDAIRERAPRTWQYLDGHGDILDARGSAIYRKNPRFAIFGIGDYAFRPWRIAICGL
YKKLNFRLVGPIEGKPVQFDDTVYYVSFDSRDEAQDALERICSIEATELYSSLIFWDEKRPIKSSVLNAVDWSRLPVPHKRRAA
>M.TspPM5I [EnzType=Type_II_methyltransferase] [RecSeq=AGGCCT]
MVFSSRLCTSPGVQGPAQKREREIHSIAISSIKGTEDMAAIEQHFPSEQLSLLAELESWRKEINRPTYHMHKWWATRLGSVFRAILIGALSEDEQDIWEG
FYQALRFPGKVIFDPFMGAGTIIGEALKLGCYAIGADINPVSVFQVRKALEPVSLEELLAAFERVAAKVAQPITQLYQTIDPESKLSLPLLYAFWVMNVP
CPACSSQVHLFDTTIFARHAYPQRVPVCQSICLHCGAVNEIRYDASVVRCRGCHTNYDPHRGPARHSQATCPSCQHTFVIVQALAQEGRPPGYHLYALLA
LRPDGSKVYLPATEDDRQKVALAGERLRSRSGPLPATPILAGHNTDQIRRYQFTSWRQLFNERQLYCLSLLLEAILAEPDEACRELLLLLFSGTLEFNNM
LCSYKGEGTGAVRHLFYHHILKPERMALENSVWGTSKSSGCFSTLFHSRLLPALRYRQRPFELALTEEKGRLTGKKIYGLSASPVTPLASHFTELSEQRR
ALVLCTDSAQVPLPDGSVDAIITDPPYFDFVHYSELADFFYAWLRPALQERYPEFAPETTRSPYEVQQRQAEAFSAALCRVFRECRRVLKAEGLLVFSFH
HSRTEGWAAVGEALLQAGLAIAAVHPIKAELALATPKSQTRSPINYDAILVCKHQAARREPLSCAEALATVVQETVTRLRSLQMAAGQQLPALSRNDCFV
LAQAQALRLVSRFRCLLRTAPAPAEPEVSLEVSLATFLELVAQEVESIWQQVSASGPATATACAPSLATTTATPPAAGQFHSTRSYPLGTGAQPQPLPWS
LQEIFDSLDGKAGNS
>NcoI [EnzType=Type_II_restriction_enzyme] [RecSeq=CCATGG]
MATAPGHLLGQIIGNVMEEALKPVLQEMADRHDLYLDSKGLRPGVRSGALVTWTDDLGNNHDLDFVLERGGSATKAGNPAAFIEAAWRRYTKHSKAKAQE
IQGAVLPVLAAWNNVKPTPAAVVAGQWTAPSLQQMRSNGFVVLHLHFPTTAQVFGGNGINIEGTGEGTPDAFWQQQCDAYTSKSEADKDSLATALRTAHA
QEFRTFVAELERRVVRAIDYVVVTPLHGHGSQYTSIENAIEAVRTYSCGEESAPFLRFEIRISYTNGDVIQATFGSSSDAIEFLDTFN
>NgoAXVI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGTGA]
MNDRYYLNTKTEYWYRIFLETEMSNNQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQDQPYSNELYEALQQNIAQDSVMASQRTSKAMNLA
SVRKAINQFVKMGFIEPFLSSYTPLSRDYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNKKEIAAMMLVDLKTFQQDY
LTETELNDYFQQGIESGFIERKYNQISYLWNLLDKLDDLKRVGDDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLAYP
VLIASHIKPFILSDDTEAYDPNNGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSDDVWRRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI
>S.Pst510I [EnzType=Type_I_specificity_subunit] [RecSeq=GAANNNNNTCGA]
MNNYSRVGIKELTEARLDANYYAPRFLENEKILAQCGLSRKRIIEVSGLCNCGATPVDVTYSNEGLGLVRTSDVRPDYFRAERVLRTKDLDLSDLRESVI
ARPGDLLYTMSGTIGYACVIPDDEMPVSFSNTIARVRIPKQSPYDAHYIAVFFNCHYGYEQSLRLVSGGMQGHVMPNPFKELLVALPNSVAQKYIGDKVR
QAERLRAWAKLAETHVNQYHQKLVPQQTGLDFAKRVRVVSPARMSERLDAHFYPGVVDEYLRLHQGKFKQLGKLCATIYNGQTQPETAGSASCKQITVTN
LSSSFLGGTPRDVEAPVKKEKFLKAYDILMCNAAHNKSYIGKDLTFVHSEAAILPSTEVMAIRVDRNQVPSSYIRAYLLTKLGYVQIQSTIRGITAHSYP
DDMALLDIYAPEVADKDKEEWFACDEKLALAGKACEVATQLTNAAKLLVEALIEGQLTEAELLTAEKALQAGNDRLDRVILSRLNTDGIDGQGPALFGDL
DELYQLLSLAEGE
>M2.SmoLII [EnzType=Type_II_methyltransferase] [RecSeq=GCAGT]
MDLKIDISEEKRSKEILEILLIDRTTNKNIIWATDDYEHFGNAYNSKHHILYHLITGENSNVIQPRVLKSKKSQVIRTKAKAEVFTPSWICNAQNNLIDN
AWFGRNNIFNIEKDKSWESTFEKIEFPKEKNKSWEKYVDENRLEITCGEAPYLVSRYDTVSGNYIDINQRIGILDRKLRVVNENVENEKDWLKWSERAFQ
SVYGFEFQGDSLLIARENLLATYCDNMMYKLKRYPTDKELIKIAKIISWNIWQMDGLVYTIPFKKPISSNNQILLFEFEEEHNDNYCVIKDWRANKKNCI
QRFVK
>CchIII [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CCCAAG]
MPITKKEIRDNALRFALEWRDASRERAEAQTFWNEFFQIFGVSRRRVASFEEPVKKLGEKRGSIDLFWKGTLVVEHKSRGGNLDKAYNQALDYFPGLKEE
ELPKYVLVSDFDRFCLYDLNENTQCSFLLNELPEHIDLFGFISGHQIKVYKDEDPVNIQVAEKMGELHDALLDSGYDGHDLEVFLVRLVYCLFADDTGIF
NAKGDFEDYLRNKTKENGSDTGSILANMFQVLDTPYEKRQKTLDEDLVNFPYVNGDLFREPLRIAHFNGAMRELLLECCLFDWSKVSPAIFGSLFQSVMD
RKRRRNLGAHYTSEKNILKVICGLFLDDLRREFEAIKNDARKVTAFHNKIAAMRFFDPACGCGNFLVITYREIRQLEIEVLQQYFMLTSKMYKAGVTQLE
TDIETISKIDVNQFYGIEIEEFPARIAQVALWLTDHQMNMRLSQAFGQTYVRLPLQHAPNIICDNALRKDWETVIPSKEHLYILGNPPFIGKQNRNAGQM
ADMDVICQPLKAKGLPNYGVLDYVALWYIKAALFIENSNVKVTFVSTNSITQGEQVAALWEFLLRKGVKIFFAHRTFKWTNEARGNAQVFCVIIGFTWNN
TTQKKRLFDYETPQSESHEIEAKNINPYLIDAIDIVVSSRNKPLCNVPEMLYGSKPVDDGNLFFDDDEKVELLKKEPKAEQFIRRVISAHEFINGKNRWC
LWLKDIAPNEWRNLPELVKLVEAVRGFRLKSKKAATVKLAEVPYLFGEIRQPETNYIVIPLHSSEHRKFIPTGYFSKDNILHNSCSAVPNATLYHFGILT
STMHMVWMRTVCGRIKSDYRYSNNLVYNNFLFPHDISNKQKAKVEEKAQAVLNARELFPNSTLADLYDPLTMPKALLTAHRELDAAVDACYRKTPFQNEL
ERLEFLFQLYSSYTQPLVPAMDAKPKRKRMGKG
>MluI [EnzType=Type_II_restriction_enzyme] [RecSeq=ACGCGT]
MSAPEVDSARDARYVEILLAPLRKCGTYLPKMGGSGEVDLAGFTAAYGADPLYHWMGLDSPLMFAAHKAAGGMTSIYRQLGIGSERLFRQVLRDELNLTA
DQVKWSYKMLPELDAEHANESVKARVLSLDGRVELEDLEDQQARERVEAWIEVQRRRLNITAPLKGAVFEVRQGYKSADSKRQNADLANAAQALGHQYLP
VLVIMSTQINEVVHARYTTGNWSVLMGTVGASDPVGSTYDFLDQVVGYDLAAFFERNKAALRAGTEGILTDLLEAR
>S.LlaBIII [EnzType=Type_I_specificity_subunit] [RecSeq=TNAGCC]
NSKAEKIILGKVESGSKSNTKDQQISSKENFEDEKLDNPFINRLIKEVEYLKIDKDKIISTKDQQISSKDQQIEKLTNLLDQQQRLALQDKKLLEEYKEE
IKELKSLMVPVRKDDKYPMVQKSKDIDNTKEELHTKNQKWWHFGRNVK
>BsrDI_(BsrDIA) [EnzType=Type_II_restriction_enzyme] [RecSeq=GCAATG]
MTDYRYSFELSEEIARWAFEIKTKNTDWFVAFSNPTAGPWKRVMAIDKASNREGEVHRFGREDERPDIILVNDNISLILILEAKEKLNQLISKSQVDKSV
DVFLTLSSILKEKSDNNYWGDRTKYINVLGILWGSEQETSQKDIDNAFRVYRDSLVKNLKEINPTPTNICTDILVGVESIKNKKEEISIKIHVSNIYAEI
YPKFTGKHLLEKLAVLN
>M.Hpy300IV [EnzType=Type_II_methyltransferase] [RecSeq=TCNNGA]
MQTLFKEITSKRYVNGNEMKENSSNVLDQYFTKPSVALKCFQKACEVIKKYENPDDFIFLEPSAGDGVFYDLFPKNRRIGIDIEPKRDGFIQCDFLNYKL
PTHQKVICLGNPPFGHRGVMALEFINHARNCDFVCFILPMFFESQGKGSIKYRVKGLNLLYSERLEKNAFIDFKNKEVDVHCVFQIWSKKYQNKKNEFSW
YKNRHKEPFGEYIKVFTVSLAKNRECGKEWIFNQKASFYISSTFYKSTQIVENFEEVKYQSGIAVVFTSADKVLNAKLKKLFKEIDWTKYASLATNSCYH
LGKSHIFQALHDHLDSLKDN
>M.Mha184V [EnzType=Type_I_methyltransferase] [RecSeq=GNGAYNNNNNNNNTGT]
NXNWVKRXVKLSTLSSLGKELRRTVKTGEMRSLXXQPVRXRKLSLQFVYLLMRIKXCHRNNLKKSTKNAVKPLTTALVLQWQLVYXVHXVLHQQQMQNKP
VQKNHLMPSRNNTVXNAMEXPLIQNVWHXWXLIHKLTSXARKAXCGVCXVTQLKKTTLXLTLKAYLKQQKQWICQLLFRHTTITRLITNGNLVHRVNTLC
TTAECLREKVNTPWKALKIQVQTLCLSTNHIFLMAKQXSPLHTKSLDRKPMTXCCNYANVKSTKXFXPVWLPTYVLIHTCVNXSNKVLKXQWXKMQPQGL
VFLKVTATLQHXPTTVLSQMTSGPLKKQXKKXRQKHNIRFTSGXIXLFFCKFNRLDFTLISSTHTKSHYFSLSPHSFSLYSSPQQITATIQQLPCLFQGX
NAIMTRYFWLEKAIENCKKXSKNNRLXXRXTSLKXLILQFLXDKTKXTXDNHQCTIGNEDSREIDIKSIPLLMGQNDRVRQKQNNEKTQ
>AseI [EnzType=Type_II_restriction_enzyme] [RecSeq=ATTAAT]
MLRGLTVELLELKNRITTSFSGTEDDLREVLDLIEQDQPVFPFNEYEHLICNLIEKGGLNYNQYIEIRSEYISQNPNLWIFEISAPRGFGEKFAQTYVKG
KCSKLKTPSKKLDPNYAGEYDLWLDGITIEVKASRAVDSNSEEPLYVKALARDTNRQFIMNFQQLKPQYCDVFIWVAVFRDEIVLWIMSSDEVEKNPFYS
KGQHRGNKGNEGQLHIKHDKIHLFSKYELKDDDLEGAIRKAAKG
>M.Mtu22115II [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGSWDGV
AGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEV
VLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGELADVDVGIVTGRNSFFTFTDAKAQA
LGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAPRLTV
NAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDG
LGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>NspV [EnzType=Type_II_restriction_enzyme] [RecSeq=TTCGAA]
MTILTIEALRTEAAIFSAAESIHPEPLLYGVTDGKAVGTYIEQKFRLYLKEHYEFVQGNSASGIDFPGLLVDVKVTSIRQPQSSCPFKSARQKIFGLGYS
LLIFVYDKIDNSTNRTATLNILHTIYVSAERTADFQMTRGIRNILANEGNKDDLIAFMSDRNLPVDEIEAGNVAAILLRNPPMQGFLTISNALQWRLQYG
RVIERAGQEDGILTVYRNNP
>TspMI [EnzType=Type_II_restriction_enzyme] [RecSeq=CCCGGG]
MIPEHGNRSDETIKELIARLPWAPESLDTLESEKEEAKKIDLLSRTHRGAEMIRYVLTHMESIFKKSFSEETTSPPSLGTFRFFPQHEQEGQKLIKHLLQ
QGINPQILFVESSSDFEKKTIKASLHAGITKKLINNYRTTDSDQLKQWINSAILLAFRTYVDLTGRKTFQESWPECVTKEDVSKFLEFTATMNLGVMADG
WWRNQVGEKAVQLFINEIEKALKKLYSKKSYRFEPNEDSPSVREYIVTNTTNETQLIFRFGEPDFSVKIRNEKMEKILILGEIKGRFDKSNLQESWYTTI
QNRMGRAGNEGKNAASVFVLVQNYFVKEEKKQINDLLNESEKKGLKLGLFSLAKLMFSQEERRRFEECIKGLIDLLELR
>CviJI [EnzType=Type_II_restriction_enzyme] [RecSeq=RGCY]
MEEKKRLALIEKQRIAEEKIASGRKIRKRISTNATKHEREFVKVINSMFVGPATFVFVDIKGNKSREIHNVVRFRQLQGSKAKSPTAYVDREYNKPKADI
AAVDITGKDVAWISHKASEGYQQYLKISGKNLKFTGKELEEVLSFKRKVVSMAPVSKIWPANKTVWSPIKSNLIKNQAIFGFDYGKKPGRDNVDIIGQGR
PIITKRGSILYLTFTGFSALNGHLENFTGKHEPVFYVRTERSSSGRSITTVVNGVTYKNLRFFIHPYNFVSSKTQRIM
>Bag18758I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CCCGAG]
MPSLSRDHRRLLENTVAQARAIAVDGARKVITDQYAVHHHEPWPHMTTEERELRNQLRAHGRQLGDRRDDRRDTQEIEHLVQACAYEHWHRMLFARFLAE
NDLLLDPDHGVAMTLEEVRELSREQNREWIEIAAELAQKMLLAVFRPDDPVLRVSLPLETRQHLEEKLGALPTAVFQADDSLGWVYQFWQRDEKDRVNKS
EVKIGADELPAVTQLFTEDYMVLFLLENTLGAWWTAKRLAEGKNPALPGYGWTYLRLNEDGSPAVGLFDGWPRAVRELRMLDPSMGSGHFLTFALPILVR
MRVEEEGLALQEAVAAVLRENLYGLELDARCSQIAAFNLALAAWRLAGEHLPLPELNLACSGLGIHAAESDWVKLAGEDGLAREEMRRLYSLFKDAPTLG
SLIDPLRLRATVFSAGADRVLSLIEEALEREQSEDVRELAIAAQGVLAAFRILASRFTLVATNVPYLGRGKQDPALAQYCAEFHVDAKADLATCFVERCM
RFCGSGGSVALVTPQNWLFLTSYRQLRERLLKSEQLDFVARLGPRAFETISGEVVNVALLGLTHRVPSPHHSFAGWDVAEARSPEDKAKGLNSTGTPNHQ
ARQLKNPSMVITTTELLHGTTLADYAGSVEGLSTGDSDRFVFRFWELPRITGDWTAFQRSPQDSEGDLGSDSVLRWQNGKGELVKSEAARVQGFQAWGVL
GVVVGQMRTLLCGLSLGQPHDKITAVLVPRDSSFLPAILAYCESEQYNDDVRKINSKLNAATAALVQVPFDLSSWQEVAARKYPNGIPAPRSDNPTQWLF
NGNVRLSGQPLQVAVARLVGYAWPRQTGSAFAKCPELNIDGLQLHADADGIACMNAIAGEASAADRLRALLAHAYGNEWSAAKLQSLLDDKSSLEEWLRD
EFFEEHCRVFQHRPFVWHVWDGRKDGFHSFVNYHKLAGPNGEGRKTLEKLIYTYLGRWIDRQSEEVRTGKEGADARLAAALHLKAELEKILAGEKPYDLF
NRWKPLNEQPIGWAPDLNDGVRINIRPWLTAKPYQSGRRDGCILRVTPRVNYGKDRGKEPHKPKDDFPWFWSWDGQSDDFLGGKDFDGARWNDLHYSLEA
KRDARLRVQSQGSSK
>CstMI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=AAGGAG]
MVMAPTTVFDRATIRHNLTEFKLRWLDRIKQWEAENRPATESSHDQQFWGDLLDCFGVNARDLYLYQRSAKRASTGRTGKIDMFMPGKVIGEAKSLGVPL
DDAYAQALDYLLGGTIANSHMPAYVVCSNFETLRVTRLNRTYVGDSADWDITFPLAEIDEHIEQLAFLADYETSAYREEEKASLEASRLMVELFRAMNGD
DVDEAVGDDAPTTPEEEDERVMRTSIYLTRILFLLFGDDAGLWDTPHLFADFVRNETTPESLGPQLNELFSVLNTAPEKRPKRLPSTLAKFPYVNGALFA
EPLASEYFDYQMREALLAACDFDWSTIDVSVFGSLFQLVKSKEARRSDGEHYTSKANIMKTIGPLFLDELRAEADKLVSSPSTSVAALERFRDSLSELVF
ADMACGSGNFLLLAYRELRRIETDIIVAIRQRRGETGMSLNIEWEQKLSIGQFYGIELNWWPAKIAETAMFLVDHQANKELANAVGRPPERLPIKITAHI
VHGNALQLDWADILSASAAKTYIFGNPPFLGHATRTAEQAQELRDLWGTKDISRLDYVTGWHAKCLDFFKSREGRFAFVTTNSITQGDQVPRLFGPIFKA
GWRIRFAHRTFAWDSEAPGKAAVHCVIVGFDKESQPRPRLWDYPDVKGEPVSVEVGQSINAYLVDGPNVLVDKSRHPISSEISPATFGNMARDGGNLLVE
VDEYDEVMSDPVAAKYVRPFRGSRELMNGLDRWCLWLVDVAPSDIAQSPVLKKRLEAVKSFRADSKAASTRKMAETPHLFGQRSQPDTDYLCLPKVVSER
RSYFTVQRYPSNVIASDLVFHAQDPDGLMFALASSSMFITWQKSIGGRLKSDLRFANTLTWNTFPVPELDEKTRQRIIKAGKKVLDARALHPERSLAEHY
NPLAMAPELIKAHDALDREVDKAFGAPRKLTTVRQRQELLFANYEKLISHQP
>Lmo370I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=AGCGCCG]
MPVSSDLTADLKKVVLSLEDDLRERVEAQAEVNRTWRDMHQQATAKERTALSWQQWRDDQVTQAAVAWVLTTVFVRFCEDNALLRPVWLTGPRSRRQEAL
DAELIYFRSHPEHTAREWIEQAIAHLRGVKATRALVDSHSALWPVSPSGQAAKELVDFWRAQTDDGELIRDFADPELSTRFLGDLYQDLSDHAKKTYALL
QTPEFVEEFILDQTMEPALQERPLDGFTLIDPTCGSGHFLLGAFARLNDKWAAQAPGEEVQARVQKALNVIHGVDLNPFAVAIARFRLIVAALRACGSTS
LEDAPGFTINLAAGDSLLHGSHQGSLDLGDFDADSQISGFAYETEDLDALRGLLMPGRYDVVVGNPPYITVKDKNLNATYRALYPSCKGTYALTVPFMER
FFELAKRGNGDQPAGWTGQITSNSFMKREFGSKLIETFLAKQDLRLVADTSGAYIPGHGTPTVIIVGRPQAPALPEVRAVLGVRGEPGQPKDPSQGLVWT
QIVENVGSSDFDGEYVSVTDLPREALATHPWSLAGGGASALTAALSQGTRVLESVIQKPIGGSIRAGADEAFMRPIGWSPRRDSVRKQLRPLVLGDIVRD
WAEAVDEQIYFPYRYGDGTPEPGALEQELWRWRAVLAARATFQGSMADAGLNWWEYMQFTKTPYKTPLSIAFAEVATHNHFVLDRGGKVFKQTAPVIKLP
KEATEDDHLALLGILNSSTACFWLKQNCYPKGGDPMGHDGARLSQQPWSDRFQFNGANVSDIPLPKRLPLERSRSLDRTAQMASERFLTGVPRDGEIPAA
GRLERARTGIDSMRALMIAHQEELDWEVYQSYGLIEQDLTYAGDVPEIALGERAFEIALARKVAAGEEQTAWFSRHGSTPITQIPAHWPDDYRALVQRRL
DLIESDKFIRLLEKPEHKRRWASEPWDKQVEKALRSWLLDRLEDTGYWFEQGRPRPKSIGQLADQVSRDPDLVSVLELWSGKRDQSVSDALTALLTTEAV
PYLAAYRLKDSGLRKREAWEHTWELQRREDAGETLAEPIPVPPKYTTADFRKTSLWQHRGKLDVPKERFVLYPDAGRETDQTLVIGWAGWDHAQQFLALA
TLIGEREAEGWDDERLVPLVAGLVELQPWVRQWHNEIDPTFGLNIADFCDEQLRDRAAQVGWTVDQLAAWRPAPTTRGRKKKS
>Bsu7003I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GACGAG]
MALIDLEDKIAEIVNREDHSDFLYELLGVYDVPRATITRLKKGNQNLTKRVGEVHLKNKVWFKEAKKGKLFDALIDIEQQVEYLSAKPRYLLVTDYDGVL
AKDTKTLEALDVKFEELPQYFDFFLAWKGIEKVEFEKENPADIKAAERFARIYDVLRKENNIIETNRGLDLFLIRLLFCFFAEDTDIFKRNSFTNLIKTL
TEEDGSNLNKLFADLFIVLDKNERDDVPSYLKEFPYVNGQLFTEPHTELEFSAKSRKLIIECGELLNWAKINPDIFGSMIQAVASEESRSYLGMHYTSVP
NIMKVIKPLFLDKLNQSFLDAYDDYTKLENLLTRIGKIKFFDPACGSGNFLIITYKELRRMEINIIKRLQELLGEYLYVPSVTLSQFYGIEIEDFAHDVA
KLSLWIAEHQMNEELKNEVHNAVRPTLPLHTAGDIRCANAIRVEWTEVCPAQGSEEVYVFGNPPYLGSKKQNKEHKSDMLSIFGKVKNGKMLDYISAWFY
FDAKYASTTNAKVAFVSTNSVTQGEQVSILWNELFKFGIQINFAYKSFKWANNAKNNAAVIVVIVGFGPLDTKVNKYLFVDETKKLVSNISPYLTDGENI
LVSSRTKPISDLPKLHFGNMPNDGGGLLFTITEYTDAINKYPELVPYFKKFIGSVEFINGGLRYCLWLNEAKYEKIKSNPLIQERISISKNHREKSTDKG
TNKLALTPWKFRDTHETTNYSIVVPSVSSENRFYIPMGLAGADTILSNLIYVIYDAEIYLLGILMSRMHMTWVKAVAGRLKTDYRYSAGLCYNTFPIPEL
STRRKNEIEEAILEILDLREEQGGTLAELYNPSTMPIELKVAHEKLDGIVERAYRQKQFESDEERLEVLLKLYQEMTER
>MtuBlII_(MtuBlIIB) [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CACGCAG]
MRLQWWVHRRQHGRRHAVVARRRLRGGICVQPAGNQRTAGELSRQEGGKVFGGGSRNTVAIFLGIKDPKHSGPCDVLYRDIGDYLSREEKLRIVGDGYLD
TVEWQTVTPNLHGDWVNQRDDAFSAWPVIGDKKAALDVTRVFANYSAGLKTSRDAWCYNFSRGALEANIGRTIDFYNSEVDRINEIRGRDAKTPPVDALI
TVDSAKFSWDRINKRQVAQGIRIEFAPAGMRLGTYRPFTKEHAYLDPNQQLNNCTYQLPSMFPTPEHGNVGYYVVGMGSDKPFSCLMLNAIPDLAFWGSS
NGQFFPRWTYEKTEPRDGELDFESTTNAEVDDHGYRRVDNITGVILKLYRDTIGDQVTKDDIFYYVYGLLHDPAYRTKYAADLKKMLPHIPTPETRERFD
QLASAGRKLADLHVGYESVKPYPLDVQLKPGADPEDRETWRVEKMKWKSKQDHSTIIYNSRVTIAGIPDEAERYLLGSRSALGWIIDRYRVTTDKASGIV
NDPNDWCDEHANPTYIVDLIKKVTTVSVETMKIVDSIVALASAGSDST
>Eco644I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAABCC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>BhaI [EnzType=Type_II_restriction_enzyme] [RecSeq=GCATC]
MLYTIDRVQYARTNNRYYFYEFMEVNNINALIGMDGKLLPPNELWNALYGIINDMEKNKIEFIKGNLQVIGIFIFYQVNVDKFIKIELDPFTKTIYGHIW
NFSELQNWFRWLNNIDGQTNSKGLGAARDTDVDSYVNGILKTLYNNPLFYDDNGLNLTKGLLNGDTTKGFDLDLFQYIPSTNEYIIYEFLKRENQNINNI
QAHPMRYCWTGKWNDNKQKFISLWNIKQYLNARLILVNYSDNTSEKISLIEVLNLDTDKGITSENKYCISRNVFLGWLHDMKNYNSNNTDYLSEFKFVHY
NQEFFEDFSKNKRRYGAEFLNIYI
>S.Pin25611FIV [EnzType=Type_II_specificity_subunit] 
MKSRIANSKYILQEKRFDGSYHNADVNVYDAVLKTHSSHSLSFYCSEIFTSGRNKREYTTKEYGFPFLSNSDASTSNPFLTCKYNSKKYGFDEKSQLKGG
MILTGRVGAIGQTSFVPSYWEKYKTMGSDNIIRIVVKPEYKNGFLYAYLASRIGNLSFLKLLTGGVQPFITDNMVGGLLIPDFPDSFQKEVDDLIQESAK
LREEASDMLTGAEKLLKKAANLRDLTTEDYDYFGPRGAEREVSCFVRNRKDITTTTINAFNLSERIRKTKASMPCATKPLKEVLLGGDTFSTGSFPRVEV
KEGYGIMLINQKDIFDTIIKGKYISKRGVKTDNMVEYGEVLVAGVGTLGENETFCRTIFANEDLVGQLVSGEFIRMKASNEVPSGYLYTWLESDYGFRFL
RNIQAGTKLCRPIPRLVLEIPVPIIDSDKMNDIDKLVKEAHTKRYEANNYERKAIRMVEQEIEKWNK
>BloAII [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAGGAC]
MDDLKSRAAKFAETWAGRGDEKSDTQQYWRDLLDKVLLIPDTSDRQVLWFERRTALDGFIDALMIQARVLVEQKSLGVDLDRPEPRQGSMVTPVQQAKRY
SDSLPPSERPTVLITCNFGTFRLYDLEADPLARTPQSEFTLADLPNHISEIGRLFTHENSRVVQQERLSVKAGRRVARLHDSLAKCFEHPDDPAEHDALA
MLTVRLVFCLYAEDANLFRPDALRDYVAASTPERLGEDLYDLFEVLDTPIGKRRRYLPESLKAFPYVDGGLFADRIDVPPLTGELRDALLDISEGFDWSG
ISPVIFGSLMEETLSHDERRKGGMHYTSVRNIHRLIDPLFLDGLKSELEQAEAKPVAGGARTNALNKLHDKIAGLRFLDPACGSGNFLTETYLELRRIEN
RILADLDKDGQLALDLGDDLNPVRVSISHFHGIEINGFACAVARTALWIAEQQALDDTESTISGLPRLPFTDTAHIQQGNALRLDWNELLPGDHCDYVMG
NPPFIGHVTKTAGQTDDLKTVWGRQYDGYLDYATGWYRKAASYLSKPDAAFAFVTTNSITQGQPVGPLFKPLHADGWHIRFAHRTFAWDAQSTDNAHVHV
IIIGLGRKAKPAPVLFEYADINGEPTARTVDNINGYLIDGPDLYVGKRSQKTGPVSPLLDVTDSGSMPLDGGNLLLADREEYAKAMADPIAARFVRPFRM
GRELINGTDRWCLWLRDAEPGELRKSSFLKKRVDACAEYRRNAPMKGDAYRHRATPWLFRDDHQPSMNYLAIPKVFSEDREYMTCDWYTPDIIAGDMVYT
SPDRDGLAFAVIESRMFMTWQQTIGGRLESRCRFSNTVIWNNLPLPALDDDTRMALIEAGRNVLATRANHPGQSLADLYDPDYMPTDLRAAHRELDKVAD
VAFGADRRLGDDDDARLKILFDSYARMTAERG
>M.HpyGII [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MVSNTTLQKNLDAFYTHPKIARFCLDLLKDLINQNLGLDLNAFHFLEPSAGSGSFVDALKGLGIADWDALDIAPKAQGIQKKDYLLELIEFNKKRVIVGN
PPFGHRGKLALDFLNKSLNEAPIVAFILPNLFKRYSIQKRIDKRAKLVLNAPLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIAPPKIRHNDFI
TYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLVKNRQYFFIKAHSKEALMIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>M.HpyHP26VI [EnzType=Type_II_methyltransferase] 
MVSNTTLQKNLDAFYTHPKIARFCLDFLKDLINQNLGLGLNAFHFLEPSAGSGSFVGTLKGLGIADCLAFDIAPKAQGIQKKDYLLELIEFNKKRIIIGN
PPFGHRGKLALKFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIAPPKIRHNDFI
TYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALMIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>SfcI [EnzType=Type_II_restriction_enzyme] [RecSeq=CTRYAG]
MNYSINEQLLRFKFLIEDSIKEGGTIGKTSMIRSSKMINLIHDATKQELICNGVNPDNIRPPLGHSKPELKIAGILKQKDQDVCVIPTGIYPTPTPITWG
PLAFNKKIDPYGFEFSEKTLIINVRSQMSSLAKNADTLFERTFAEAQNLHLRYPNAVLGEVYLIPVNEYDDALVSKHQVGFKTRQTDLEKYISFFTEINN
RSIGEPPHSYERCALLIVDFNQPQPLLFSNSDELKAAGYISSDFDIEYANINFQNFASDILSIYDQRFDINYLI
>MscI [EnzType=Type_II_restriction_enzyme] [RecSeq=TGGCCA]
MGDMAYRDRPLNAEEMEALRLVLSTYRDSSGQNQTKYGSMPGFRDFERGLASVLGGTAAENKGVFDIIVTPSDGSTAFGISCKMARFAPKAQNAAFVELS
NAAAKFRAHLLERQINWATDPMLAGPAIIELVTKWHTDDANEHGLDLDKSAYAVLSRSSDWSTYQLSTFPLDLYGFNPIGDIAWTATTKRIDGHVEINGQ
PHLLWQWYPTSGGQLKWWPPLSWATWSTEPFTLEEPPLVRPVERAEEYFPDLWPHGFTPSA
>AclI [EnzType=Type_II_restriction_enzyme] [RecSeq=AACGTT]
MGKLKKILAGTAGRNTGHAYEEYLADNINSLNVPLNISIQPLPIVGSYQNVFSSNNEAFTLITYLAKAYNISQITRIYAVATGGLATSANSQGVHFNGQI
IKKCKSDILIEIEDQSNQIHRIGISVKQCNNQRPLNAQVFCSTASGFSNLLRQHNIPVSILAENELRKFCGHPGSSPIDHNIHLTRVIDHRRYFWEELDI
NASQEWQNIFDNYNAEIARILLQKAYSNEPYPPEFLLHKTKRTSGNQEIAIFEIADLIVKSFQYQCFTTSLYRVKKGTFKEAKTMGDTHEAPSFGIFQMQ
RLGNTQNAHQLQFNLKAGYFYHI
>ScoA3III [EnzType=Type_IV_methyl-directed_restriction_enzyme] 
MIPLKRPGISANLEKRLTGKTKTLTDNLADPKNAREAWKSARTIRRDLKVLLASMAAGFARCMYCGDSLGTDIDHFKPIARDPLSAFIWANHFLACSHCN
SNKKRDQYPCSPSGECLLVNPSKEDPYEHMNLNLPTGKYQGQTQKGKETIRVFGLSRPDLELGRAHAYVRCESMLRDWSTRVGNGQEEAASAVLKSLMIQ
PFADVLYAMIKKSEAPGAPNLFEADVLTALRSVRERIETQDVAASFRNP
>M2.Pru9I [EnzType=Type_II_methyltransferase] [RecSeq=ACGGC]
MYKLELWFGDFVESYDDLKLLRQKSLKSHLNFKYKDNFESKKNLDLLDNVMERVQKTELWDKRIPKMLNGYFEDMFNIIDNCYNTLKSGGFCAIVVGNSA
YGGEIIPTDLILAEYAKSIGFKVDKIEVDRYIITSSQQYEKTKKNGKYLRESVVCLIKK
>Pse18267I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=RCCGAAG]
MLNAVEIEEAISLLAEQEFDPAEFPFAFLEAFGNKKATIDRLRRGNTNSSDIEGGLLQRNNIHLAVCAEGKVGATLAALRESSATTRGKAKFILATDGIT
LEAEELGSDVAPIVCAYKEFPDHFGFFLPLAGISTVKQVRESAFDIKATGRLNRLYVELLKDNPDWGKASRQHDMNHFMARLIFCFFAEDTEIFSGVDLF
TSTINQMSDRDSSNTHEVLGELFRAMNTKYADRDSAKIPRWADAFPYVNGGLFAGSTEVPRFSKIARSYLQSIGNLDWKKINPDIFGSMIQAVADDEERG
ALGMHYTSVPNILKVLNPLFLDDLREQLEKAGDNARMLLNLRKRIAKIRVFDPACGSGNFLVIAYKEMRAIEAEINQRRGESHQKTEIPITNFRGIEIRD
FAAEIARLALIIAEYQCDVLYRGQMEALAEFLPLGAENWITCGNALRLDWLAICPPRGKGVKLAADDLFSTPLDQAEIDFENEGGETYICGNPPYKGGTE
QGAAQKADMQILFSDDFKTWKSLDYILGFFLKARRFLDVANASVAFVTTNSVCQGEQVFRFWPNIIRESVRINFAFTSFKWKNLASRNAGVTVCIVGLSK
KNGAVKLFDGYSGLVREVDNINAYLVPYYDVYIQNSTKPIQGLEPVVNGSKPADGGGLVFSAEVARNLRDKVTDRKNVIRPFFGAHDAMHGVGRYCIWAT
SEDYKVLREIPAFQSRFAIVTEMREKSNKELTKEGASVPYSFQQIRQSGGEVVFQIPRHSSEERVYYPVGTLPEGAIVGDGGFAFFDPPLWNISILVSKI
ALCWIGTVCGKIKTDYRFSNTLGWNTLPVPKLTEKNRADLSRCAEDILLAREAHFPKTIAELYDPENMPDDLRRAHERNDEVLERIYIGRRFKNDTERLE
KLFDLYTKMTAGNAPAKKGGA
>Eco2149II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAABCC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>SspI [EnzType=Type_II_restriction_enzyme] [RecSeq=AATATT]
MSKAAYQDFTKRFSLLIKKHPNLITMTLSNIFTMRLIGNKTHGDLAEIAISEFINQYMYDFKSIHVGKDLYRAKSKEEDITVENEITKEKFPISLKAYGD
GPLQLSTDKNFLMYPLLEEIGAFINAKEKIEEIFANEAFSCFSEINVLPLIYDEKRQRCNILVFDAARARAETAYIRKETEGSGRKHPAYRFFDKNKNYI
CEVRYGNAAANALQRGLWTNTKNATSFFDSVTNGWVDYSHNLVLVKLLSHALVSSRKGHEAALEEIKKDILQLKQTNGINV
>NgoMVIII [EnzType=Type_II_restriction_enzyme] [RecSeq=GGTGA]
MSNNQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQDQPYSNELYEALQQNIAQDSVMASQRTSKAMNLASVRKAINQFVKMGFIEPFLSSY
TPLSRDYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNKKEIAAMMLVDLKTFQQDYLTETELNDYFQQGIESGFIERK
YNQISYLWNLLDKLDDLKRVGDDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLAYPVLIASHIKPFILSDDTEAYDPN
NGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSDDVWRRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI
>HpyGI [EnzType=Type_II_restriction_enzyme] [RecSeq=TCNNGA]
MDLEQTFLKIIEKKHKELNLGQDYNAIFSKIRDFEANAIGQIGEEFLKSVLNAIDEVINDGIIHDEYDIMTKSGVSFEVKTARKGRTNNTFQFNGINPRY
NYDFLICLGVCEDQLLYRIFKKDEIHYIHKERKYFMKQNEFKKQLVPMNPDNQVNYKLTLNIKELKEITNLIKELERILELD
>M.Rsp7740I [EnzType=Type_II_methyltransferase] [RecSeq=TTCGAA]
MFAPPPEASMPNNEFGDFQTPIELARALVNTLPRRQWTRVLEPTCGVGNFLSVIGERHPDTERVGIEVQPEYAAAASIFGRVITASIFDFDLARDIAWTS
ESGPTLVVGNPPWVTNSQLSVLGSSNRPARANTGNVRGIDAITGSSNFDVAEFIWIKLLAEFADRPVTVAMICKTQVARNVLLHCARHGLPITGSSLRPI
DAKKWFDAGVDACWFVVELGTDATDHTAQMYSSMDALHPSSRIGVVDGQLVADVDAYERSKQFDGISPLIWRQGIKHDASAVMELAENDGPRTKLGAAVD
VETDFLFPLFKCTDVYRDKLSVVSRWMIVPQSHTGDDTAQLADSAPKLWKYLTDNATALDGRKSSIYRNRARFCIFGVGPYTFAPYKIAISGFHKIPQFR
MVGPYDGKPAVFDDATYLLPFEDPAACAVAHALLTGPEATDLIAALAFWDSKRPVTKKLLQRIDLSAIARTTDRRTLRDRARDVHTDSSSIDQAIDDFTD
RT
>SphI [EnzType=Type_II_restriction_enzyme] [RecSeq=GCATGC]
MTSKDPIVLSADQIAWLRQLKMSKRAALVRDYILEYGAVTTGKLAELGYSHPPRAARDLKDAGAGVVTIMVKGPDGRRMASYAFNGKANEDGAGRVVIPK
AFGEALKRAHGGKCAVCYGDFSERELQCDHRVPFAIAGDKPKLVQEDFMPLCASDNRAKSWSCENCPNWELKDEDTCRSCFWASPENYTHVSTRPERRIN
LLFQGDEVEIFDALKNAAANEGVSLTEATKRKLAD
>M.AorT14III [EnzType=Type_II_methyltransferase] [RecSeq=GTCGAC]
MRNVGHPHPSRRSGRVSSPSSACAPTPPTAARFRMSASSSESPEGCSPSMYDVTLDQIAATCLALSVDRHCLSSAELAVLPTQPVESLDLSQIRRLILDG
EDPLGQAFCAARTAEERRPRGQTFTPAPIVRSMLEWTKGRVTPARVVDPGCGSGRYILAALRAFPHAVGLASDLDPYATLMTRANARVLGLESRLKVIVG
DYRALRLPKIEGVTLFLGNPPYVRHHNINARWKTWLGATASQMMGMKASKLSGLHAHFFLATALYAQAGDIGSFITSSEWLDVNYGQLIRELLIGPLSVS
SLHILSPTSLPFGDATVTGAITCFEVGANAPEVQIQTVDSVDALGDLSTGFPVSRQRLAETSRWSVITRVTPRLPDGYVELGELARVHRGTVTGANKIWV
TPRDAVDLPDDLLFPAITRARELFAAGERLSTSDGLKAVIGLPTDLDILDEPDRKRVDRFLRKAKRLGVANGYVARNRRAWWSIGLSSPAPILTTYMARR
PPAFVRNAVDARHINIAHGIYPRETMSDSDLDALAAYLRVNVSTTSGRTYAGGLTKFEPREMERLVVPDLPLLRDKGRHDSRSAAEMVGV
>M1.CatHI [EnzType=Type_II_methyltransferase] [RecSeq=CTCTTC]
MLDLTKITTSLTLSKEVTHTDAKKEPLHNWFPYLEGFSETFIENILNSMSESPKFLYEPFSGSGTVPVFSKRNGIHCLYSEVNPFLLELTDLKISTFDLT
DIDKASFKKELYTISQNIETILNNTTENKNLKEYYDKVFGRSIYFEKENFSAVLKLSNYIKSIDNKNTKQFLRIAVCNCLLPASLLKRAGDIRYKRGKEK
NNIPSIITLLKAKLLVIADDLNEVNSSENKITLERNTNAKVYMNGYEEKIDAIITSPPYLNGTNYIRNTKLELWFLGYLKEKKDLSRFRKEVVTAGINDV
SKEEKKIIIPFIQDILDNKDLWYDKRIPKMITDYFFDMKIVIENFYKYLKKGGKVFLDIGDSVYANQHIPTDLILIELFKNEGFTIIDNLKLRDRRSKSG
RIVKQCLIVAEK
>Lmo11238I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=TAGRAG]
MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITRAKSKFDNGEPFVIKNKLHYEEIEGDVVIAIDAIAQSIKEQKSKPRYILVNDYLDIA
AIDTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDNPDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRT
AEDGSNFNEVIKELFAILDINELARNGKDEWLLEFPYVNGKLFGESHVDLTFSKVSRELLIEAGELLNWNEINPDILGAMIQSVASAEDRHVAGMHYTSV
PNIMKVIKPLFLDDLTESFETLKLRSEENELKDITEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLGDLQNNDSM
PLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELLPLKDAGNIVCANSLRVDWDQVTHFSKEDEIYIMGNPPYIGAKKQTPLQKE
ELESALDGNKKYKKIDYIAGWFYKGAKFIAKYNASLAFVTTNSISQGEQVAMIWPDIFKFAQIFFAYGSFKWSNSAKNNAGVTVAIIGLTSKTEHKDKFL
FSEDIKKKVSSIGPYLAEGDEIIVATANSSIAGFPNAYLGSLPLDGGNFIFDETDRNDFIQKYPALSNLVKKYIGSSELIKQEYRYVLWMNEEEYIEHKD
NPVISERVEKVKHFRKNGGQSAQGAVDRSYEFFTKNQREQAISNNRKHSKKEMKTIIIPRVSSENRSVVPMGIVGEDTVVSDSATAVYNAPMWLLGLLES
RMHMVWLRSIGGKLKTDYRYSAGLVYNTFPIQNLSTQRKNEIARVMTEILDLREYEGGTLADLYNKDSMPESLRKKHEELDGIVDRAYQQRPFESDEDRL
GTLLKLYQEMTTND
>Bbr02I [EnzType=Type_II_restriction_enzyme] [RecSeq=GATC]
MEDKANKAWTDLIQEYQIDNAIKQHGEFIIQAKDIKKYREPRLMAKWDSSESLPTILKKSDINILPVSRSAYVLSNFKLYQPFPENDINTVKYVPPLPLE
SIDFNNLSTESNAINAMLITGILDDFLGTNNTVETFNGRMGTNDFSFTVNLTNGGKRQVDVQSAQLEIDGGFENDDSIIIMEAKNVPHPDFHIRQLYFPY
RLWCERVRKPIRLVFSQYIDSIYHLYEYEFAKEDDYSSIHLIQQRNYSFQSSAISWDDIQRIIDSNSPEIDDNYDDTSIPFIQADLFTRLISLIEQLDKV
PNMTSAEIASSMEFDIRQASYYSSAGEYLGVIERPLRGHSQLSLTGIKLMRLKLRERRLELIKLIVRHNIFHDLLSQIIDTGEFPSREAIIDRMRRYNVC
KDATTLYRRASSVSGWLRWIVTLPDTTEEFE
>BsuRI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGCC]
MGKNSKAIGNNHVKSVYQALLQSLKSKSVNGFSKITIETISFIKNLYPEIDSVTSKFDNSRPDQSKDLTLYLKSGETISLNLFLIKKGRRIQPKNAGAKS
FLEKYFLSAEMQKIFNKEFERYYLDYLKEVVEHKKGTHYITDKRELKRLVSSHFPKFTEEINLYRDKFLFNLRETCFTLLQQFYNEKNIGFTHAFNVFFM
VNDTNIITSYGKDENDVKVEKFAPASPSLKDIELYKTGKSTVGIKFGEVGLTLRFKFESDPWKSIKLATGYHEFPKEKERVNVNLKTMRRMEKLLNKHEY
AKTSNNSNAIGKCHEAWTYYYFLKAFPDVIQVDPKQCVELINTYFSSINQNTLKKLYSSTSTIVDAITEKLRQKYHDYIIESIELIPDAYIKDRLDTGDL
QLVLKVNNNIIVENISLKALAKRNSKITTKNPGMGSILGPTYFNMGSMESVINEVKNKFTIGEFNHRKSLEILSYEFGMKLDSATQEQLRRGIHNLLGKA
MIAITIYGEGISFCKEPSEIDGEVKVHVNVPSAIQNTLTWNNELESISLRAKFSKSQKHGWSSIKLTSECQLESRK
>LlaI_(LlaIB) [EnzType=Type_II_restriction_enzyme] 
MHNQEIYYGAPGTGKSRKVKDTLLAGVPQSKVFRVTIHPEFTYSDFIGQLLPEATSKGVEFKFHRGPFTDALVEAYADTSAEVFLVLEELSRGNVAAIFG
DIFQLLDRDDYFVSEYPVNNKAIAVELSQATDDIKLPSNLNIIGTVNMNDQNVFPMDTAFKRRFDWKYGSTHPQVKLGTTSVDTKLNNPLIPINYSGLGG
DFEVNWLSFYSALNKFITDKNEGLGKKEDKQLGQFFIKFSKDDILNSNSDTIGNPSFTPLDKTTAQDSIIETLRNKLLLYLWQDVQGVSGFASSSSLFAD
NITSFDTLYEDFGNKQVFSDDFLDKFLSGTNRTIYNY
>M.Mtu28I [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGSWDGV
AGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEV
VLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGELADVDVGIVTGRNSFFTFTDAKAQA
LGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAPRLTV
NAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDG
LGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>S.Xor2286I [EnzType=Type_I_specificity_subunit] [RecSeq=GAGNNNNNNCCT]
MAEHFHTLFTTEPGIDALKQTLLQLAVMGKLVPQDPNDEPASELLKRIAEEKARLVVEGKINKQKPLAAFDEEAAPFELPAGWKWTSLAQVAVVNPRNAA
ADSLEISFVPMTFIGTRFDGQHAQETRLWAKVKQGFTHFAEGDVGVAKITPCFENSKACVFSNLVNGLGAGTTELHVVRPVCQTLDPRYVLAYLKSPQFL
LVGETKMTGTAGQKRLPKNFVETNPFPLPPLAEQHRIVAKVDELMALCDQLKARLGEARQVHGNLANALIGQALNGEKEPRAEAVYFSAYLISKLASRRT
FGRVAHMKLLFLADSHLGLGLMDGYRRHAAGPLDTSIYRVEERAAKEGLYSTSIEVLKSGQEKVSYHIGANISRAVETAASALGSARKELDRLIALFEGR
KTDDLEAVATLYAVWNDALAGGLHPSDEWLINEFRSNWHEAKERFTPDVLGRWLGWMRDNGLVPTGNSATTKSQAAFLFN
>CviQI [EnzType=Type_II_restriction_enzyme] [RecSeq=GTAC]
MLTETLVRRFVKKAIKNVIDMDKKRMKAEIKLAKAQAKEQEKEAKRIIRENNRIFKKALKMNLPDNEPISDDDIVYDDSHIEKGVQSQHHGFVFENELRH
KVFLIPTRSNDVNTHDISKNQNRFDAIENISIKTTGKKDICCGDIIRFYDYDFSDANTMIVGQYTQTTTHKKIDRIIEIKYDKKLHEVLFGTITRDELIA
YVDMVKALPRGRISKSKRDEILSMKKELEKTHGMYIQISPKIDSGNQRRVQCRIRNVDDLVEKNPENLLSISTTGVVRGIAINTIIASYRRNRTARLR
>HpyF17I [EnzType=Type_II_restriction_enzyme] [RecSeq=TCNGA]
MGRIMAKRKSDIILKSVNDLKDEIDYKDFEYKEYFNLLCELVPNNSLEKLEINAIDEKNMKNEGLVYVFVIQGKIFKIGHSITPITKRVQSYNCGKVEYR
KNGTCSTTNYFVLQSLLKINKIVQVYAFFPEQPTYTLFGKTYQDSFSTSKRAENVILENFIKNHNKKPIGCTQT
>S.Kpn39II [EnzType=Type_I_specificity_subunit] [RecSeq=GACNNNNNNGTC]
MSQYRAYPAYKDSGVEWIGQVPEHWKVARVKRLASLRNERRNDVSTDTIYIGLEDVEAGSGQYKPTNGSSRQSEDSTVGIFYEGDVLYGKLRPYLRKAII
SEMAGCCSTEFLVLRAEKTEPRWLQEWLLTPDVTHQIVSGCEGAKMPRADWGHIGSIEVVYPDQPEQAKILTSLDRETARIDALIEKKTRFIELLNEKRQ
ALITHAVTKGLDPNVKMKDSGIEWIGQVPEHWKVARVKRLASLRNERRNDVSTDTIYIGLEDVEAGSGQYKPTNGSSRQSEDSTVGIFYEGDVLYGKLRP
YLRKAIISEMAGCCSTEFLVLRAEKTEPRWLQEWLLTPDVTHQIVSGCEGAKMPRADWGHIGSIEVVYPDQPEQAKILTSLDRETARIDALISKAEQSIT
LLKERRAAFITAAVTGQIDLRGKQ
>S.EcoNIH2II [EnzType=Type_I_specificity_subunit] [RecSeq=GACNNNNNNGTC]
MSQYRAYPAYKDSGVEWIGQVPEHWKVARVKRLASLRNERRNDVSTDTIYIGLEDVEAGSGQYKPTNGSSRQSEDSTVGIFYEGDVLYGKLRPYLRKAII
SEMAGCCSTEFLVLRAEKTEPRWLQEWLLTPDVTHQIVSGCEGAKMPRADWGHIGSIEVVYPDQPEQAKILTSLDRETARIDALIEKKTRFIELLNEKRQ
ALITHAVTKGLDPNVKMKDSGIEWIGQVPEHWKVARVKRLASLRNERRNDVSTDTIYIGLEDVEAGSGQYKPTNGSSRQSEDSTVGIFYEGDVLYGKLRP
YLRKAIISEMAGCCSTEFLVLRAEKTEPRWLQEWLLTPDVTHQIVSGCEGAKMPRADWGHIGSIEVVYPDQPEQAKILTSLDRETARIDALISKAEQSIT
LLKERRAAFITAAVTGQIDLRGKQ
>NcuI [EnzType=Type_II_restriction_enzyme] [RecSeq=GAAGA]
MKNYVRNINLGNSSLKFIDERLQSENYRGIHLSQHNRYDLPKLMEILTLLNRYAPNQSLMQIRTTDISKRPYNIPEEQSYAEFCNEAKNLTNIGTQDAMR
KNLFVDFARMGLINRYNANKELTNPFKKSTTKYVSLSEMGLKLIDQKLDILNKNLIFSKSLNRLLTGFVEDVLSLLTNSDLKEISFDEFMLFVSAINCNF
SFSISIEQCESLIKEYRLLSRVQKNAVIDTLKSELIPDNFNGDKKDKRDYHNWANENQQIWALFENIPFFIMEKDSKKLILITSDIDLSKYSKSKMKRSQ
QAKNDYFKHHKVNKTKGYELDHIIPLLEAESVNEYHYLDNWLNLLYIDGKTHAIKTQSGSRYYIFSFDSNNFDQVHFANTQEEKLSICNGDQALFNKEQV
PRIYNYNQNFLQTKTNNS
>Mtu2279II_(Mtu2279IIC) [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CACGCAG]
MAIFLGIKDPKHSGPCDVLYRDIGDYLSREEKLRIVGDGYLDTVEWQTVTPNLHGDWVNQRDDAFSAWPVIGDKKAALDVTRVFANYSAGLKTSRDAWCY
NFSRGALEANIGRTIDFYNSEVDRINEIRGRDAKTPPVDALITVDSAKFSWDRINKRQVAQGIRIEFAPAGMRLGTYRPFTKEHAYLDPNQQLNNCTYQL
PSMFPTPEHGNVGYYVVGMGSDKPFSCLMLNAIPDLAFWGSSNGQFFPRWTYEKTEPRDGELDFESTTNAEVDDHGYRRVDNITGVILKLYRDTIGDQVT
KDDIFYYVYGLLHDPAYRTKYAADLKKMLPHIPTPETRERFDQLASAGRKLADLHVGYESVKPYPLDVQLKPGADPEDRETWRVEKMKWKSKQDHSTIIY
NSRVTIAGIPDEAERYLLGSRSALGWIIDRYRVTTDKASGIVNDPNDWCDEHANPTYIVDLIKKVTTVSVETMKIVDSIVALASAGSDST
>S.Maf25I_(S.Maf25IB) [EnzType=Type_I_specificity_subunit] [RecSeq=GATNNNNRTAC]
MTGSRQRIQPTQLLRVWVRDVRRLTPGHAAAIANLGALCNERRIESARLASCRDALLPLLMSGIDGLPAGR
>M.RspPBTS2II [EnzType=Type_II_methyltransferase] [RecSeq=TTCGAA]
MPNNEFGDFQTPTELARALVNALPRREWTRVLEPTCGVGNFLSVMARTHPETERVGIEVQPEYASSAAQFGRIITASIFDFDLARDIEWSSGPGATLVIG
NPPWVTNSQLSVLDSANRPARTNTKNARGIDAITGSSNFDIAEFIWIKLLTEFADRPATIAMICKTQVARNVLLHCARHQLPVTGSSLRMIDAKKWFDAG
VDACWFLVELGPGQTDYTAETFPSIDASQPDNRIGVVDGRLVANVDAYQRSKHFDGTSPLVWRQGIKHDATAVMELAENGGPRTKLGTGVDIEADYLFPL
FKCTDVYRDKLDSVSKWMIVPQSHTGDDTEQLTHTAPKLWAYLNANAAALDGRKSSIYRNRARFCIFGVGPYTFAPYKIAISGFHKIPQFRMVGPYDGKP
AVFDDATYLIPFEDPATCSVAHALLTGPEATDLIAALAFWDSKRPVTKKLLQRIDLATIARATDPVVLSERSAAAAAAHGLVFEAASLTHAVDLARGKKL
>Eco0398II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAABCC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>Lba2029III [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CYAAANG]
MDKNVIKKYAVWAREQLIEKVTARAVKYQIVDGQEMDGSLEIINGEVLSETEIKQRKALIQKIKTDGYSQVMEEVAYTWFNRFVAIRFMEVNGYLPSHIR
VFSDENGAFKPQILAEAIHLDLPGLDMDKVMQYKDEANEEDLFKYLLMVQCNALNEILPQMFQKIEDYTELLLPDYLLREGSVIEVMINPTNGIPESDWM
DQVQIIGWLYQFYNTETFDSIFDGDMSRKKVTKALIPAATEIFTPDWAVRFMVDNSLGRLWLDNHNNAELKKKWRFYLEEVDQDEEVEIKINHIKKSYAD
LKPEEILCIDPCMGSGHILCYMFDVLINIYEDYGYVTRDAVKCIIEKNLWGMDIDERAAQLAYFAIMMKARQYDRRFFSKNIQPHVYAIKESNGLSEDVI
KLLDREKETVKEIIDSLKDAKEYGSLLKLNLQIEQVHAIKRKVEEIIETSSSGDIVEQLNAVMLKDRFLPLLEIAEVLVQKYHVAVTNPPYLGNSRMNTN
MSDFIKKNYPDEKADLAMAMLNRVTWGFTKENGYSSGISTVSWMYLKSFEKFRKKMVSDSNFVCLTDYGTELFDGKIGHLPIASWVTRNCTVDQKIHSVR
LVDFCYSRRDEKEPEFFNGENHYIAQQTDFIKIPGEPIAYWASDSKISAFENGVLIDDIASVKIGMGTGKNEVFVREWWEVLFDEIDFSLKRFEDLEDSN
GVYFPYNKGGDYRRWYGNLQEVVWYNEAGRKKMFSMSGHRENGGRNGYFKKGITWNFIGTSKFGVRLMPTGCLFDVAGSALFTSDEYLYYLLGFLSSSVC
NEILKILNPTVNYQAGNIKSLPVIMDREAEIKSIVLENVDIAKRDWDEFETSWDFSRHPLISYAAIPGDNLYLADIYKRWSDDKYELFARLKSNEESLNR
IFIDIYKMQKELVPDVEDNDVVVRKADLQREIRSLLSYSVGCMFGRYSLDVPGLVYAGGDWDISKYKTFQADVDAIIPICDDEYFEDDIVGRFVKFVEVV
FGKDTVEDNLKFIADALGGNGTSREVIRNYFINDFYDDHVKVYQKRPIYWLFDSGKKNGFKCLIYMHRYQPDTIARIRTDYVHEQQARYRTAIEEIEKRI
ESTSGSDKIKLEKKLNTLRAQDDEIHSYEEKIHHLADQMISIDLDDGVKHNYDLFKDVLAKIK
>MnlI [EnzType=Type_II_restriction_enzyme] [RecSeq=CCTC]
MDFNNFLNKATLNALLVAFANAKPITKPFIGFVIESSYKNSAYSPIELTDDIYQQNNQLLSQFTNLDFQLNGKKHQFFIQSDLSCDDFFALVYKNILIKG
STIDNDEFSKLILLSFFALRGSPDFKLNFYSLDLPRQIVSKNYLDNLFKLSTNVSDLRQLNLNFRELQEQFITGENERNTQFRINLRYFYDNFSDDLAKI
NLFKSNILKHNANLIKTKNIAQESRTFIERLNFYRDNVLNQTKTQHEIEQLRKNLGFIYDDTIDEKTTRNQGIVQYVRAYFPDECACCKNQYNIKDRSFT
YRHSDRYYLEVHHVISFASDRTLDQIDNLVKVCPTCHRALSKNRADEQYQKELISEILINAPKAKEFCLNFTDENDCIQFIYDRLR
>Esp3007I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CAGAAG]
MQCVTVGFYYNTPRNAPGGCYLAANYSTHRGIEMVCSVFLYPFPYAIMDLSNSEVNTMTDMEQRNAAKTFAADWNGRGYEKGDTHSFWLSFLQDVLDVEH
PTSYIEFEKEVVINGSTKFIDAYLPDTKVIIEQKSLERSLTKASKQSGGSMLTPFEQAKNYANYLPADEYPKWIITSNFGEFLIYNMNKPGEAPTRIALS
ELPKRYNEFSFLIDTKKDYLKHEMEVSIKAGELVGQIYDAFLALYPDQKDPRYLHSLNVLCVRLVFCLYAEDAGIFGRRDMFHDYLNSFKPENMRMGLIE
LFRILATEPDQRDPFANENLLEFPYVNGGLFSTEEDIIIPPITDHIRDLLLDKASLGFDWSDISPTIFGAVFESTLNPETRRSGGMHYTSIENIHKVIDP
LFLSALKTEFAEIKKLKVAKTKDTKLRAFQNKLASLTFLDPAAGSGNFLTETYLSLRRLENQVLLELTKGQIMFGAAGISPIKVSIRQFYGIEINDFAVT
VAKTALWIAESQMLKETEDIVRLNLDFLPLKTYVNMTEGNAVTMDWNSVVSSDRLNYIMGNPPFIGARMMNVSQKKDVSSVFQGSKGVGNLDYVSCWYKK
AADYMKSTDIHAALVSTNSITQGEPVSILWNALLSMGVVIDFAHRTFIWDSEAHLKAHVHCVIIGFSCGRGTSPKRLYTDGILQEVDNINAYLVNAPNVL
VASRKTPLSDVPPMVFGSMANDGGFLFLSREEHDDLVSRYPQLTGCIKRFVGSREFINNVERYCLWLKDVTPSLYRNVPEIEQHIAGVKEHRLSSNRKST
RDLALTPFLFGEIRQPESKYILVPRHSSEKRRYIPLGFMSPDVICGDANMLIPTDSLYHFGVLTSNVHMAWMRTVCGRIKSDYRYSASIVYNTFPWPDPS
EQCIKKIEETAAAILAAREQYPDETLASLYDENYMPVELRTAHRANNRAVWEAYGKAWDITSETDCVAHLMKLYQTLVSE
>M.MtuHIII [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRD
FASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFL
LSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGEL
ADVDVGIVTGRNSFFTFTDAKAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWW
STPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDV
DLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>PaePA7I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] 
MNAVEIESAVSDLALQPFDGTEFPFAFLQAFGNKETTIKRLRSGASNKSDLGGVLQTNNIHIAVAQPGEVTKTLAALKASSATTRAKARFILATDGEGFE
AEDMETGETVACTYQQFPDHFGFFLPLAGITTVKQVRDSSFDIRATSRLNRLYVELLKDNPDWGAAAQRHEMNHFMARLIFCFFAEDTDIFNGEGLFTAT
IEQMSARDSSNTHEVISEIFRAMNTKIADRANAKLPRWADGFPYVNGGLFSGSVDVPRFSKIARSYLLHIGSLDWTKINPDIFGSMIQAVADDEERGELG
MHYTSVPNILKVLNPLFLDDLRMKLEEAGDNPRMLLNLRKRLARIRVFDPACGSGNFLVIAYKQMREIENTINERRGERGRKSDIPLTNFRGIELRDFSA
EIARLALIIAEYQCDVLYRGQKEALLEFLPLSSQNWITSGNALRLDWLSICPPTGTGVKLHADDLFETPLNQAQIDFENEGGETYICGNPPYLGSKWQSA
EQKAELAGIFDGRIKNWKSLDYVAGWFMKAADYGKHTPAAAAFVSTNSICQGQQVPILWPAVFATGCQIEFAHTSFKWANLAAHNAGVTVVIVGISSNAR
PVRRLFSISEESGAVLRETDNINAYLVAAPNIEIQAQSAPLSDLAPMQFGNHPYYGAALIFPVEEARELIEETPDVARYIRPLYGAQEFINAVPRACLWI
EDHEAAEAMQLAPVATKATEVVRSRRAAKQDKAAQKLSETPYRFRDQVTATRHLLVMPRVSSESRPYLPVGLLGPECVVQDQALALYDAPLWNLALIASR
LHLVWIATVCGKLETRYRYSNTLGWNTFPVPTLTEKNKADLTRCAEDILLAREAHFPATIADLYDPGNMPHNLRAAHERNDEVLERIYIGRRFKNDTERL
EKLFELYTKMIAISGKTSKARKATTA
>Bsu3610I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GACGAG]
MALIDLEDKIAEIVNREDHSDFLYELLGVYDVPRATITRLKKGNQNLTKRVGEVHLKNKVWFKEAKKGKLFDALIDIEQQVEYLSAKPRYLLVTDYDGVL
AKDTKTLEALDVKFEELPQYFDFFLAWKGIEKVEFEKENPADIKAAERFARIYDVLRKENNIIETNRGLDLFLIRLLFCFFAEDTDIFKRNSFTNLIKTL
TEEDGSNLNKLFADLFIVLDKNERDDVPSYLKEFPYVNGQLFTEPHTELEFSAKSRKLIIECGELLNWAKINPDIFGSMIQAVASEESRSYLGMHYTSVP
NIMKVIKPLFLDKLNQSFLDAYDDYTKLENLLTRIGKIKFFDPACGSGNFLIITYKELRRMEINIIKRLQELLGEYLYVPSVTLSQFYGIEIEDFAHDVA
KLSLWIAEHQMNEELKNEVHNAVRPTLPLHTAGDIRCANAIRVEWTEVCPAQGSEEVYVFGNPPYLGSKKQNKEHKSDMLSIFGKVKNGKMLDYISAWFY
FGAKYASTTNAKVAFVSTNSVTQGEQVSILWNELFKFGIQINFAYKSFKWANNAKNNAAVIVVIVGFGPLDTKVNKYLFVDETKKLVSNISPYLTDGENI
LVSSRTKPISDLPKLHFGNMPNDGGGLLFTITEYTDAINKYPELVPYFKKFIGSVEFINGGLRYCLWLNEAKYEKIKSNPLIQERISISKNHREKSTDKG
TNKLALTPWKFRDTHETTNYSIVVPSVSSENRFYIPMGLAGADTILSNLIYVIYDAEIYLLGILMSRMHMTWVKAVAGRLKTDYRYSAGLCYNTFPIPEL
STRRKNEIEEAILEILDLREEQGGTLAELYNPSTMPIELKVAHEKLDGIVERAYRQKQFESDEERLEVLLKLYQEMTER
>BsuMI_(BsuMIA) [EnzType=Type_II_restriction_enzyme] [RecSeq=CTCGAG]
MTFIKRLEDAYETLLGNYPAGVSSTSTSKYNEIRKIVSEAFLIGENEVYVTGTSRRISNLDTRFAQGNQRNKHTRMAVAFISIPSVDDSELDELIIRTRN
SAITTSSKFCNGEERGTIFDGILLFLVFEGETKVYPLAFLVFENDFELKEKAEELIPGIELKEYPRANQSPAQENNKSAKNEDEESAKSYVVFLDIEEDG
SIVEFVEDKDKTYRIGDMIWTASHTNGSSAITRRLEVIEVVENLVVCKIKHKYNEPVDKNSLLKFVNIEQDLISFLDLHPNVQNGSEGFVSGDIVDENAT
TSSDDLPEDFENN
>GauT27I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CGCGCAGG]
MSSSNAAVTPAVALRALRERWAGVPAAERANAQSYLRDLCEALGVPAPLPAGSGYEFELPVKLITRDGTETTGFVDCYKVGHFILEAKDVQGGASDVALR
RAYGQARQYAAHDPSGTAPPYLLVLDVAKTLLVYHRWGGVYQGFAAGHRIDLPTLDQRPSDIELLRDIWTQPTKRDPRQHAQAVTQEIAAKLATLAATLE
DRGFGPERVARFLMRVVFSCFAEDVDLLPREAFRQTVQNAGVQGDAALFQRALGSLWQTMDSGGLFGFENILQFNGHFFKDAEVLPLEREEIALVLEAAR
ADWRDVEPTIFGTLLTRALDPVERHRLGAEYTPRAFIERLVRPTVEEPVRERWTAVQAEVLQLRESGKAKDRAAAEQRLREFLGWLQGLRVLDPACGSGN
FLYVTMHVLKDLEYEVVRELEALTGHAELRMQEIGPKNFLGIEVKPWAREIAELTLWIGFHQYWKRHHHVQPPEPVLMDTGTLELRDAVLAWDAVRHVPE
KDRFDPTPRITHTVTGELVPDPAATLPYMEHVGARQAPWPEADFIVGNPPFLGQFRQRESLGDGYVEALRSAYPGVPDAADLVMYWISKATRAVESGRTL
RAGLITTQSITQKQNRKVLEDAAVRGLRPVWAIADHYWNDGSDDARVRVAMTVFAREPAAATLVTVDGEAKVVNTVRVPRLNTDLTVHADVPSAAAVALQ
ANAWLCSNGYKPHGTGFLLSDEEARRLLSLDPRNAEVLRPYRNGMDLATQPRRVWIIDFGFADESEARTYPLLFDIVRDRVKPERDANARAVYRTYWWRF
GEARRDWRSFVAGLPRYIATVKTAKHRFFTFLESTVAPDDKLTCIASSEGFVLGVLSSLIHSTWALAAGSRLGIDGTPSYDKGTCFDAFPFPDCSADVRN
RIATIAERIDAHRKSAIERSAKVGMTVMYNVIDKLRVGATLTSKEREVHEVAACGVLRDLHDELDATVAEAYGWSWPEPPALILERLVALHDRRVEEEAG
GTIRWLRPEYQRPRFGGATDGANVAPTLDLPATPTTLTGAGTVIASAPWPSDAIGQITVLRSMAAMTPVSIEEAVQRLVGAKRDIVHRHLETLAMLGEVR
DVGDGRYAVTGS
>S.PaePAI [EnzType=Type_I_specificity_subunit] [RecSeq=GACNNNNNNGATC]
MLRNGRLFGHRVETGFPDLPILEVSLRTGVRVRDMDNLKRKQVMSQKEKYKRAVKGDIAYNMMRMWQGAVGPVPEDGLVSPAYVVVKPYAEANSTYFSYL
FRTAAYMQEVNKFSRGIVADRNRLYWESFKQMPSLVPPRPEQDQIVTYLRTQDAHIACFIRAKRDLIALLTEQKLRIIDHAVTRGLDASVKLKPSDIEWL
GEVPAHWEVKRLKFLAGNITSQTTTKADDEIYLALEHVQSWTGVARPLGGEVEFASTVKRFVADDVLFGKLRPYLAKVTRVVCAGVCVSEFLVLRSRQEL
ILPAYLEQLLRCKRVIDLISSSTAGAKMPRADWNFIGNVRLPIPRKDEQEAILSHIGRETKDLDETIARAEDEIKLIREYRDRLIADAVTGQVDLRGWQP
GPDDVVSDDDLAALGDDDAGSSDDEEADAED
>PliMI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CGCCGAC]
MTAASVASIDPAAFIARWAPSGGSERSNYTLFLSELCDLLGVPRPEPAQADTSRSFYVFERDVTFHNSDGTTSTGRIDLYKRGCFILEAKQGVEQADAVE
ILSSTPAKRRKGHGTRGTKAYDDTMLRARSQAEQYAKALPADEGWPPFLIVVDVGHSIELFADFTRSGKTYLPFPDPKSYRILLNQLAHDDIRHTLKTVW
TDPVSLDPSRRAAKVTRELADRLARLAKSLEASKFDPGRVAQFLMRCLFTMFAEDVDLIPRESFTNLLKSLRDDVANFPEMIRSLWVSMDKGQFDPVLRK
KLRRFNGGLFEDCEALPLTRDQLELLIEASQAQWRDVEPAIFGTLLERALDPRERHKLGAHYTPRAYVERLVMPTIMEPLREEWDTVRAAAFTEAGRGHR
EAAIQILRDFHRQLCETRVLDPACGSGNFLYVALEHMKRLEGEVLNTLHDLGYKQREIITVDPHQFKGIEVNPRAAAIADLVLWIGYLQWHTRTRNLDDI
SEPIIQNFHNIECRDAVLAWDAIEEVRDEHGQPVTRWDGRTMKKHPVTGEDVPDDTARVPVVKYINPRKAEWPKAEYIVGNPPFIGNSRMRHALGDGYAE
CIRQTYNRLPETCDFVMYWWDRAAEFTSDNQVNRFGFITTNSIRQKFSRRILEQYINGPVAALSLIFAIPDHPWVDSSDGAAVRIAMTVAAPGTKVGRLN
NVASESIGVDDVAEVNLRERIGRIQTDLTIGANVAGTLTLKATQGLSCPGVKLHGAGFIVSPEEARHLGLGSVPGLELHIRQYRNGRDLTASPREVMVID
LFGLGVDELRQRFPAVYQHVHDHVKPERDQNNRAVYRDSWWMFGEPRAAFRPSLKGLARYIATVETAKHRVFQFLDASILPDNMLVVIALNESLHLGVLS
SRIHIVWSLAAGGTLEDRPRYNKSRCFEPFPFPEMPNPRTPRIRELGEQLDAHRKRQQAAHPDLTLTGMYNVLEKLRSGELLTAKEKVIHEQGLVSVLKQ
IHDDLDAAVFEAYGWPVTLTDEEILERLVALNAERAEEEKRGLIRWLRPEFQNPSGGQAIQPELELELETEAEDEEETAAATTKKGGKAKKGAGKTAPGG
KTKAPAKQPWPTKLSEQVQALIQKLQTADSPLTAQELAKAFTRANADTIDELLETLVAIGKARQVGDDKFTAV
>Pam7686I [EnzType=Type_II_restriction_enzyme] [RecSeq=CCATGG]
MAKDVASSGHLLGQLIGDWWERYVVLPMLQSVATELNMFADNRYIKRTCRSEKIHWTDYDGNTVDYDFVLEINGTPTKKGTPVAFMESFWRRGARHSKDK
ARDDTNKLLPMRETYPSARFLAIAASGEFTEPARDYVKSRGVSLFFVPKSKIVEAFSLSGFNIDYDDRATEETKNEIAKIFQNKLDPKTASEVALKLREA
VGDGAFTGFIHTIVSSLSAQPVEIEIRQATLSEPAIFRSIEEAAEFLVTPSFTPTEVEAGYTYKVTYSDGSDFFREIPTLKALKQLNSQLLELLSHMSSV
TEKPRLHI
>M.Hpy300II [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MTSNATLQKNLDAFYTHPKIARFCLDLLKDLIAQNLGLDLNAFHFLEPSAGSGSFVDALKELGIADCLALDIAPKAQGIQKKDYLLELIEFNQKRVIIGN
PPFGHRGKLALDFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIAPPKIRHNDFI
TYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALVIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>Lmo11AII [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=TAGRAG]
MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITRAKSKFDNGEPFVIKNKLHYEEIEGDVVIAIDAIAQSIKEQKSKPRYILVNDYLDIA
AIDTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDNPDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRT
AEDGSNFNEVIKELFAILDINELARNGKDEWLLEFPYVNGKLFGESHVDLTFSKVSRELLIEAGELLNWNEINPDILGAMIQSVASAEDRHVAGMHYTSV
PNIMKVIKPLFLDDLTESFETLKLRSEENELKDITEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLGDLQNNDSM
PLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELLPLKDAGNIVCANSLRVDWDQVTHFSKEDEIYIMGNPPYIGAKKQTPLQKE
ELESALDGNKKYKKIDYIAGWFYKGAKFIAKYNASLAFVTTNSISQGEQVAMIWPDIFKFAQIFFAYGSFKWSNSAKNNAGVTVAIIGLTSKTEHKDKFL
FSEDIKKKVSSIGPYLAEGDEIIVATANSSIAGFPNAYLGSLPLDGGNFIFDETDRNDFIQKYPALSNLVKKYIGSSELIKQEYRYVLWMNEEEYIEHKD
NPVISERVEKVKHFRKNGGQSAQGAVDRSYEFFTKNQREQAISNNRKHSKKEMKTIIIPRVSSENRSVVPMGIVGEDTVVSDSATAVYNAPMWLLGLLES
RMHMVWLRSIGGKLKTDYRYSAGLVYNTFPIQNLSTQRKNEIARVMTEILDLREYEGGTLADLYNKDSMPESLRKKHEELDGIVDRAYQQRPFESDEDRL
GTLLKLYQEMTTND
>PmeI [EnzType=Type_II_restriction_enzyme] [RecSeq=GTTTAAAC]
MTTNSPSDVGMIDECLSIVRTSLARCFQQQAPSIQATWPLSGRAVSEIGGRLVESFVLARLPHELSTTPFDGQILCEIPESGRAMEDIAVTFIGPHGRAR
LLIDVKGHNEYRTGSRPNLASIRKCLELYRSSSHTVDELVVFFCRYRPSVHPDHHAQAVEYHVLPESFNEQGIFLLRALSESNLDPANIGSGGQLLLARE
NNIRLVNRSRSEFVQLLEGLQSRLQRGRSTV
>PvuI [EnzType=Type_II_restriction_enzyme] [RecSeq=CGATCG]
MKKNRYESIIEGIFLDKYVDGNDIVEFNRTDIISKSAELDINLPKNIGDVIYSFKYRASLPVSITQKAQNGKEWVIKNIGRSLYCFQQVNYSRILPDMML
STIKIPDSTPTIVAEHAFNDEQALLTRVRYNRLIDIFTGAVCYSLQNHLRTTVPSVGQIETDEIYVGVDRLGRQFIFPVQAKGGKDELGIVQIEQDFLLC
RHKYPNLICRPIATQFISNDKIAIFEFVLENNEVKKLQEKHYLLVGKGQISVDELSNYNF
>LlaI_(LlaIA) [EnzType=Type_II_restriction_enzyme] 
MRKLLLQDGSYQTKFNKIDVTPPTGVSKLEYIETKLLQEINNKNPRLNILAIEYLDINNEFTGFIGGTYSALIIDEENIPSIPTSIIDDSRYFTDAIGNV
IRQRIMAYVFTSPATKDEGRNITVAQDIFPRLLDYIDQYINSPSYTYANHPFYYISLMTPSGQLQASLLSNYARLNQLDFEYIELFPTGVNFSKMPRDVE
GAIDFIANLPRSRKTISDVQVTDEYEFDVINKKLTILSGTLLVNLTHNNFGRKVERTRRGNAGFKGSEEKFYWLDVLSMFELALRNNYEIDYSQLWDWYN
QNQRVNSFGNGDKFPRFESLLKYFDKKTLKG
>M.HpyCH4V [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MVSNTTLQKNLDAFYTHPKIARFCLDLLKDLIHQNLGLDLNAFHFLEPSAGSGSFVGALKGLGIADCLALDIAPKAQGIQQKDYLLELIEFNKKRIIIGN
PPFGHRGKLALNFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLE
>BseYI_(BseYIA) [EnzType=Type_II_restriction_enzyme] [RecSeq=CCCAGC]
MKLGEINLKKFLEEKKGIVYGELVQDAKLRWYTREYEYAILKDNKMEIWPKGKVANKIVLPTKIILDSELVTFFGLYSGDGAKGTEIINKPGRITTSISF
SQKEPHLIKFAINQFRKIFGDNIWFDFSLGEDSAYFMDEDGHNRIKSVLNDDVPLVMESLNELNVNLSAADIRYLNEQRNVSITNEEALAFHYQYNNEMQ
KYLIDVKMNDLNDVGITLGPNDRVNASLRRPFKKGARTMGGSSRSDELYVKGVSLFGELFLKILHSIEESILNDTQESTDTLIKWDGKPSTIGEVIDLKN
HFLESPYAEINGSKPILEEEALYLIGKYPRGSLVKLNKRLRQTPLWLYAAGLYLAEGSTAKEKMFQMYTSRARGLSLSFTSSEPYSLEIIIKALELLFFD
EQILSSWKVKVGSQYFPELVTTGLKLGVPMLRGGLSGDGKLRTMEISLSIKRWALEIVPFFSKYEDRFSHVEPTGAGVARIDFSGSSKLCKWYFGLIIYS
AFKNTTKDPKGEF
>HpyCH4V [EnzType=Type_II_restriction_enzyme] [RecSeq=TGCA]
MLEKVFQEITNKRKFFASSSTGEQFENQFRNELKKHFSEINGDLIEGLSHIEEKPNKEIKTTFNQLKKQVLEKNHPETLKNPFSKLTSHFLYQPFGSQNY
PDFLVFIFDHVVGIEIKFSKNDKGEKNLQTSRPMWNSNLPKPNAIYVYGVANADITFFKGSDILSYETREVLLKYFDTLDKDEESLKNALKDLENPFGFA
PYIRKAYEHKKEFSNHHQIESFFSRNHILREQNVLEFLKTLTH
>Hpy299IX [EnzType=Type_II_restriction_enzyme] [RecSeq=CCGG]
MSLADIILERFKDFMREQPEPYKSLQVFYAQEKERFLNDKMNDYIKQNKGKEEASILARQGFVSAIGRVLEKIIELLLKDFCIKNNVKMTNDKILRDKHI
NGELDKVKRALLVHFGGYSVLPDIVLYQTNKDNVKILAILSVKNSFRERFTETPYWKLKLLQSPITSHIKVFMITPDNDDEISFKDRPKKARIVMEHELD
GLYLAKSHFDQSSKIKGIENLLEDLKRLL
>FspI [EnzType=Type_II_restriction_enzyme] [RecSeq=TGCGCA]
MLTNNEIERLRQAIIATIASPVIGSIEDYTWEAIFHYVKDIPLSDPALGRSKLLYDAVDVVTKTGWSLKSLQLKSLNFKSPFLFVIQRADILKKSVQLGF
PGLTEQSSPDELGAAIIQHWNEKIILSQAAQSVVNSYEGILLKTIKGYEYIYCEFPLDPLDPSTFSWAWTVDKTTGGAGVGLQGSIVGKTELVWYKNQKQ
LFRARTIPAQAVRITVERTRLTLDRYVKTVIFALQDQINMQFSENEPEE
>Mtu2279II_(Mtu2279IIB) [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CACGCAG]
MRRRAGEVTSAEGKQQVIAELYEKFFRIGFKKQAEALGIVYTPVEVVDFIVRAADFVSRKHFGRGLTDEGVHILDGFAGTGTFITRLLQSDLITAADLTR
KYSQELHANEIMLLAYYIAAVNIESTYHALAGKTADADAYEPFPGMALADTFQISEAGTRWTRSCSRTTTLGSCDSWRRRSA
>ScoDS2II_(ScoDS2IIC) [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GCTAAT]
MRLTQIKEFPIPDATDEQELELAKKVDTLMEEIRKENGNQELIDTLNSQVDEFVMDLFCLAEKEKKIIREFEI
>S1.RbaNRL2II [EnzType=Type_I_specificity_subunit] [RecSeq=ACGANNNNNNGRTC]
MQLSKVVEVLPGVPFRSRIESEKNGACVVVQARDLVGDGTVDLTGAARIAAPPSSTRGFLKPGDVILQPRGNRYSVGKMDDVTGPTVAAAPLYILRRRAK
DVDPDFLVAFLEAASTQSILRQDAVGTHVPQIPRQALEALHIELPDLSSQIRLADLARLERREIEVMDRLRVARGRLFDLALREIAKKSRKRASAPGSIP
GLKGAPTP
>NspHI [EnzType=Type_II_restriction_enzyme] [RecSeq=RCATGY]
MNEEQNLVEIIQRQFRQNSTQLQVFNLLSDEKWHCRECEGKKIGSNQYAGGGGIQGLQRGTRSRPGLVIETTKNYCPTCQQVRLGDQWTGEIKSANSASN
IPASLVERILQVYSYTDVIEQRQREKHELVIDHRFPMERWGASEPPHLTSMSDDEIKQKFQLLKKDASGNHNLLKSRSCERCIKTGKRGTPIGIHFWYQG
GEDWPSPHQRGAEAEEGCVGCGWYNFEVWRNAINQTLTQSDQYK
>M2.Sgr13350III [EnzType=Type_II_methyltransferase] [RecSeq=GCTCTTC]
MNPPGALRPAPASGTGLCLLLYTVATTHERFGREPVTQQSTLDLGVTFRDNRNQAVHSWYPYVEGFSADYVRQKIARHEGVANVYDPFGGSGTVQVVASH
SGIDSSYSEINPFMSFVAETKVNAARTARENQSEFQAAAKQFLKHLRSDGLEHEAKLVDLSAYHEAFPRRDFFEEDHLRTLLAVKDLACRTGDGRAWLRD
LLLLAVASNAVASSHMTRRADLRRRRANEYVTRVVDVQGLVHSSVTQMMRDIQMIPDRTGSMTKLSEDCREIRHGEAGRFDLAITSPPYLNGTNYFRNTK
IELWLLDFIKAEKELPGLCRKAVTAGINNVSRTRTLEHTYPSVEEVASTLDVASPDKRIPLLVRQYFSDMTQVFQAVQHNLRTGGEFIFDIGDSKFYGVH
VPTDRLLVQVAEHVGFEVTSDEVIARRYSRDRSELRQVEIIFRKA
>BsmI [EnzType=Type_II_restriction_enzyme] [RecSeq=GAATGC]
MNVFRIHGDNIIECERVIDLILSKINPQKVKRGFISLSCPFIEIIFKEGHDYFHWRFDMFPGFNKNTNDRWNSNILDLLSQKGSFLYETPDVIITSLNNG
KEEILMAIEFCSALQAGNQAWQRSGRAYSVGRTGYPYIYIVDFVKYELNNSDRSRKNLRFPNPAIPYSYISHSKNTGNFIVQAYFRGEEYQPKYDKKLKF
FDETIFAEDDIADYIIAKLQHRDTSNIEQLLINKNLKMVEFLSKNTKNDNNFTYSEWESIYNGTYRITNLPSLGRFKFRKKIAEKSLSGKVKEFNNIVQR
YSVGLASSDLPFGVIRKESRNDFINDVCKLYNINDMKIIKELKEDADLIVCMLKGFKPRGDDNRPDRGALPLVAMLAGENAQIFTFIYGPLIKGAINLID
QDINKLAKRNGLWKSFVSLSDFIVLDCPIIGESYNEFRLIINKNNKESILRKTSKQQNILVDPTPNHYQENDVDTVIYSIFKYIVPNCFSGMCNPPGGDW
SGLSIIRNGHEFRWLSLPRVSENGKRPDHVIQILDLFEKPLLLSIESKEKPNDLEPKIGVQLIKYIEYLFDFTPSVQRKIAGGNWEFGNKSLVPNDFILL
SAGAFIDYDNLTENDYEKIFEVTGCDLLIAIKNQNNPQKWVIKFKPKNTIAEKLVNYIKLNFKSNIFDTGFFHIEG
>CkrI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] 
MWCCGVYDGFMLAKPTVTLDRGTIRRGLQEMSAHWLSRVDGWRQAGAGHIEKSYAQQFWSDLLRCFGVIPERVKLFERDAIRASSGRTGYIDLFWSGVVI
GEAKSPGRDLDAAERQLLDYLSGGSISQHEWPRYSIVSDFEHLRVAKLGEDGWSVSFTIGEIADHVDQLIFLAGQETVSKKEEEEASIHASRLMADLYTA
MVGDDDADEAVGDEAPTNPEDEDWQIQKTSVFLTRLLFLLYGDDAGLWQEDLFYRFVLYDTTPGNLGGQLSQLFQVLNTPESRRRHVPESMAAFPYVNGG
LFADTWPAEFFTEGMRDALLAACRFHWTRISPAVFGSMFQLVKSKDARRAHGEHYTTETNILKVLEPLFLEELRDEAERLIGNKSTTARELRQFRDSLSQ
MVFLDPACGCGNFLVVAYRELRRIETDIIVALRAKEGQQTASMDVSLDQRLSIGQFYGFELNWWPAKIAETAMFLVDHQANRRLAEAVGDAPERLPITIT
AHVRHGNALRMDWAQEVPSTKGDTYVFGNPPFIGQYTKTAEQTADMKQVWGKDYDGYLDYVTGWHAKAKDLLAERSGDFAYVTTNSITQGQPVPALFGPL
FREGWRIKFAHRTFAWDSEAPGKAAVHCVIVGFTRNSKASPRLWDYPDVKGGAVEQRVGQRINAYLIDGPNVLVAKRMKPLSSVISPAVRGSQPTDGGNL
IVEKKAYGEVAADPIAAKYLRPFRMGRELVRGLDRWCLWMGDDDFNPADISKSSVLKKRVEAVREMRLGSKKKATQQSANTPHLFQEDHQPDCAYVGIPA
VVSETRKFYTAAHLGAEVIAGNKLYTAVDPDGLLFGLISSSMFITWQRAVGGRLKSDLNFANTLTWNTFPVPELDEKTRESIIKAGQGVLAARARHPDRS
LADAYNPLAMDPALVKAHDRLDRVVDKAFGAGRKLTTQRQRLEILFARYTELVG
>BtsI_(BtsIA) [EnzType=Type_II_restriction_enzyme] [RecSeq=GCAGTG]
MKITEGIVHVAMRHFLKSNGWKLIAGQYPGGSDDELTALNIVDPVVARDNSPDPRRHSLGKIVPDLIAYKNDDLLVIEAKPKYSQDDRDKLLYLLSERKH
DFYAALEKFATERNHPELLPVSKLNIIPGLAFSASENKFKKDPGFVYIRVSGIFEAFMEGYDWG
>M.TliII [EnzType=Type_II_methyltransferase] [RecSeq=GCAGG]
MHMYFIENPDFPAKEINDASKKEKGPGRPPYWEMVFWWTRKPLIGARGVITGAFLPYNTDPKRFKTAIGLNEKTPHRVNPKIPPEWEKYFRGKKLLDPFA
GFGSIPLEGLRLGLDVTAVELLPTAYVFLKAVLDYPKKFRKPLVKDVEKWGNWITEQLKNDPEIRELYDDDVAVYIGTWEAKCPHCGRWTPIIGNYWLAR
VKDSKGKYQRLAYMKPKKNGDEIEIEIIDLNKIVGDVSKAKVNTNTGKIIFEDEKTAKEIQQMIQNGELDSDIARVEGNRVIFEVPKPNIEAKKNQMACL
YCGNLVKYADSEGNHYLAKPKGGDKNDFDFYVKWALRKYHEGDERFARQRILVRVKVIDGDLVFEPATEEDNEKLLKAKEKVKEMLEKKDPDVPTEPIPL
YENRRITPILSAEKWYQFFNPRQLLTLIKIVKLIREVGKRVEEEKLKEGWDKEKAFEYAEAVATYLSIAMLKYAYYNSIVTRWDSTWWKIGETMSTRGIA
MNWNWTESPWFGNFGGIIKTFPAILRGLEYLTSTLSSSQRTLADFAGNSIRVLQGDATSLNLGEKFDVIVTDPPYADDVPYTELSDFYYVWLKRALSDVE
NGKLVPRFHKKAFFKEIKIGGKSIFREIPTQWQEFAKKEVSTNPGRFMDQENKKEVAEKHFEDLFSQAFVSMKKHLKEDGLIVTYYAHTDPASWANLLEA
GWKRAGLQITRAIPLTTESETNIVSRGKLSLDTSIVAVWKNVERKEREISIRDLLQKMRVKGEETARVLIDHGYQELDLLYGTMAGILEEVTKYEEVYGS
TGSISTKELLEKYVYPSTILSIINAIATGESGRSVSSNYGVFYTAYKILFGSRSLSPNDIVLLRLATGVDIDKITASQKKKGLAVLKETGSSDKKEFTLY
TVDLIAKKNELPKPYDIQALLSERGINMKDPKIRTSVDALHLLEYYAYILPKEEFSRILEELKSKAPAEVEEAINMAFLIHRYYRRVLGGDVREYERELK
DKGLLELYLINRLVGILSGGVVV
>LlaBI [EnzType=Type_II_restriction_enzyme] [RecSeq=CTRYAG]
MNIDQVANKMKRDLELAITDQIVDGSKVNKKGKLFLNGAEAKQSLIRSSKLINYVHEFVKHELIRNSVEESLIFPPLGQTNPEIKLTGMFKQKDQDVCVK
PQGVLPERTLIGWGPMINSGLYCDYGRAYAERVLSINVRSQLSSLDKNSDTLFERMFAEALNLHELYPKIVMGEVYVIPVYEYDDQAMINNQVKFKSRRT
NLEKYINFFYYLSGRDEQDLEEDKQKYERCALVIIDFRGDQAKVYKNTAELKARGLVRNDFEVELAELSTDKFIEDLLLIYNNRFPGSVAKFENQTRPL
>BanI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGYRCC]
MAQLKYNKDIDELERNAAKWWPDFLAKKESSTSIIPKLVESQDAFISLLNLSKNNPFDIFQLIDASKFPPNLFLKHLVVLTDFGGEPLNRLNQNFDSLFP
MIPYGIHYITKVLGKFEFFWNEKKYEYVFQELPVTSLTNSKLKIDGASISKTVPLSDLYKDVIVLLMFGANAVNSEVSEVLMKCEVGNLIGKTDELKKFI
KERYIFVSRITGGAEANTLGQVAQTHVIDFLRTRFGSKGHDIKSNGHIEGVTHNDGQTLTTFDVVIKKGSKSVAIEISFQVTTNSTIERKAGQAKARYDM
VSDTGNYIAYIIDGAGNFQRKNAITTICNNSHCTVAYTEEELNVLLKFILEKLE
>Maf25II_(Maf25IID) [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CACGCAG]
MEWQTVTPNLHGDWVNQRDDAFSAWPVIGDKKAALDVTRVFANYSAGLKTSRDAWCYNFSRGALEANIGRTIDFYNSEVDRINEIRGRDAKTPPVDALIT
VDSAKFSWDRINKRQVAQGIRIEFAPAGMRLGTYRPFTKEHAYLDPNQQLNNCTYQLPSMFPTPEHGNVGYYVVGMGSDKPFSCLMLNAIPDLAFWGSSN
GQFFPRWTYEKTEPRDGELDFESTTNAEVDDHGYRRVDNITGVILKLYRDTIGDQVTKDDIFYYVYGLLHDPAYRTKYAADLKKMLPHIPTPETRERFDQ
LASAGRKLADLHVGYESVKPYPLDVQLKPGADPEDRETWRVEKMKWKSKQDHSTIIYNSRVTIAGIPDEAERYLLGSRSALGWIIDRYRVTTDKASGIVN
DPNDWCDEHANPTYIVDLIKKVTTVSVETMKIVDSIVALASAGSDST
>M.PfrJS9aIII [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MTLFASKAAPTDDKLRGGYYTPTPLAEFVAGWVARAGHRLLEPSCGDGNILEPLSRLSPAAQGIELFPEEAAIARSRTGADVEIGDFFSWFTPRRHGQYD
GVAGNPPYIRFGNWTEPERSIALDLMRSAGMRPSKLTNAWVPFVVASVLACRTGGRIGLVLPAELMQVGYAAQLRQFLIDRCAEISVVAFKRLVFPGILQ
EVVLLLATVGEGPARMQVVEVDDAASLAGFEIGAAEVRAPLHEREKWTKYHLSPAQIEEIRALRQDERLSPMEHFASVDVGVVTGRNSFFVMSSAEAEDN
RVSDLTIPLVSRSAQLDGTIYTKRDHAASTHRTELLDARGINIHSHQGLQQYVRLGEIEGVHEGYKCRIRKDWWVVPSIWQADAFMLRQIHTHPRVIANA
TSATSTDTVHRMRVAEGVDAKKLATAFFNSVTFALSEIVGRSYGGGILELEPSEAEELRLPDPALVTDELVEQVDRLVRSKKIDEALDLVDQALLVDGLG
FTTDEVTLARSVWTTLRDRRNSRAKKKQD
>McaTI [EnzType=Type_II_restriction_enzyme] [RecSeq=GCGCGC]
MTKEEFESYLDDIASKLRDEARKTPFAAAKQFEQRVREITKETIQAPGIEIDFNPHPQAFPDIEIGQFGIEVKFTTNDEWRSVANSVLETNRIESVQHVY
IMFGKMGGNPDVRWGEYEKCVMHVRTSHVPRFEVQIDATRSLFEIMGISYDQFRVLEMHEKMQYIRKYARSRLKNGERLWWLEDSPGEAHTLPMQARLFT
ELEQSEKIRLRAEAILLCPQIVQSGRARHKYDDVALFMLTYHGVICHQTRDMFSAGSVGNPENDDNGGLYIARMLKLMEAELEKAAARMDAALFEEYWGV
AVPPEERIAEWLRRADKFASGIWKPSEELFDGRYAQPRGA
>Hpy8I [EnzType=Type_II_restriction_enzyme] [RecSeq=GTNNAC]
MIPTQLNEIAEFLKTNPYHLSQPLQDGRLNSSVNEEEILNTIKDYFPIQLPKAREWWDFSFKKNDIFYPVNIKTTTTKTADNLNCKLGIYYALCGLVPEF
NNEIAWEKYFQKLHKDLGKNTDRDYYFLIINKNDPKDVFINSLKGIQTLQPNGNNLPFQCKWDNNREIVQRNFDESKNFILSTLAKSVTLRANIYLTFKE
FFGEFFE
>S.LmoJ1816I [EnzType=Type_I_specificity_subunit] [RecSeq=GAAYNNNNNGTC]
KXKGKITNXCHNKNYHNPKNRTTCFXNTTEPCNNKCXNAXTNNXRKHIGXFTPFTFQHXSSIYNEHRCYPYNKKWNKXNXXKXHRPHNRDEXFIPFXKSS
INHRKHRKKXCHCQNNPIANTSSIYKRQDNEQKQKKTCXXIDNQSCCPLLKLTVFPIILGRRLFHNIKRPRQQNCLGLYSDIILQYIFQLILQLLLLXIY
LKLLPLISPFQIDLNVXLTNEIXECLKHSLKIHLSLGLKEVVQPFDLQPFLHTYQSICPLYEKHRXPFXLNVKWMDCMVGKGKKRVHSYGLQPLYTELSH
LFRXRRNQLLSQIDQQEQQQQEFQSXFQVRWNQILLFQQLLVLLSHLVKYHALDGIHLLLQSSGTXFLSYHIHLREVRREVVYGIILHSQNKDGLHGILR
MGSFL
>M.SnaBI [EnzType=Type_II_methyltransferase] [RecSeq=TACGTA]
MFYYYGRKKQIAKHYPAPNYGCIIEPFAGSAAYSLFSGNWRNQVVLIEKDPKVASIWEWLISEATEQKIKNLPDLKVGEKSSEFLHIIHAATKMAFKYKT
ITVTPVLARNWEISRRVMAENLQKIKHWQIICGDYTVAPDIEATWFIDPPYKSEPGMGYGFSSALINYEELAKWSLNRQGEVICCEGEYGDYLPFETLLD
LKGVAGKSSREKIFYRTPHSPMQLRLVA
>AhyYL17I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=YAAMGAG]
MTSLHDDWLSLIDISGPFMAVPVLKEAFPQGLEELDGTKRKRLRQAYEEWRDALEQDDPQLDELHSAWIDEVLSRGLELDEDGKGDVLKRADWCAINLKA
VLPDHGVALSPDQAVVDEQRANKPMMLIHTYAQDVDLDATQKLDGWAATPADRMVQLCRTLGCRLGLITNGERWMLVDAPVGAVTSFASWYARLWGQEPI
TLQAFVHLLGIRRFFVDESEQLPALFDGSLKYQDEVTDALGEQVRRAVEVLIQSLDKADQDRNRELLHDVKESELYEAALTVMMRLVFLLSAEERGLLLL
GDERYEANYALSTLRMQLRKESEEILERRWDAWSRLLAIFRAVYCGVEHENLRLPALGSSLFDPDHFPFLEGRAKGSNWRIDTARPLPIDNRTVLLLLEA
IQQFQGRTLSYRALDVEQIGYVYEGLLERTVKRTDEVTLELDATKKSQSPWVKLAELDAASSDSAERLVKLLQERSGCSVSRVRNSLFKPVDDILADRLL
TTCQGDIQLRDRIKPYAHLLRTDPWGYPLVYPAGAFIVTSGSNRRETGTHYTPKSLTEAIVTETLTPVAYFGPAEGTPREGWRLKSPAELLDLKICDPAM
GSGAFLVQACRWLADRLNEAWSLAEANGKAVSVDGIVLDALGTCEQLPRDIDARIVIARRLIAERCLYGVDLNPLAVELAKLSIWLVTLAKGRPFGFLDH
SLRCGDSLLGIHQLDQLTQLSMKPSAKGQLRLFGKDIEKAVREAIELRQRLREMPIRDIHDVEVMAQLNADARHRLEVPGCIADALIGEMLVSGGNISVI
DNAMTSLAIQAGQAIASDRDSLHSIIRRARNALSVELPAGIPTRIPFHWPLEFPEIFTEANSGFDAFIGNPPFLGGKKIAGISGRKYLNYLISSLGFGVK
GSADLVAFFFLRASSMLKKSRVLGMIASNSISEGGTREVGLEQLNKNGFVIYQAINSEVWPGSANVVTSRVYIYNGEWLGAKTLNGSVVDYVSPYLTSRD
NLKPVKLANAAGRVYQGSVLLGSGFIIDYDIAIKFIEKNIALNAVIFPYLSGQDVNRSPTHKSDRWVINFWDWSKEKAEKFSPELFSILERKVTQERSEM
KSMGGAVETFWKHLWYRSELYHRLGYGTLFDEHPIEWNPNDIPPKQVIVFATGATKYPSFTFVPNGYIFANTLCVIASESHALFACLSSDIHTVWAWEYG
SRMKQDLRYTHGDIFETFPFPLEALDKDDENLSSLGESFFNLRSHYMVENNKGMTKFYNDLHNPAENDPAIQRLRDLQIEINKAVANAYGFNELDLEHGF
HEVAYLPENDCLRFTISEAARAEVLRRLSELNRQRYEEEVARGLHDSKAKGPSGRASRVRRTTTATAVQPSFDFDTQDTTTVTGATPAMVILNFLSAHTG
WHTKSDILAATSIKGGQWNAAIADLISSGKIDRQGERRGTRYSVVREAV
>R2.BsuMI [EnzType=Type_II_restriction_enzyme] [RecSeq=CTCGAG]
MEISKQTSDLLLSLEKKKGTLPKFSVLRSIPRNRIIYGAPGTGKSNYLEREVGKIFGDNPYVFTRVTFFPGYTYGQFIGAYKPVPIYKKLSGEEEIFSSN
FRDKMENFEPMIDYQFVPGPFIDVLIKALKNRYTNFILIIEEINRANAASVFGDIFQLLDRNKNGESDYPVTFGPDIMNYLARNGIKDEMIKLPSNFFIW
ATMNNADQGVLPLDTAFKRRWSFEYLELEKYRKAVDSWKLSLRYKGHNKVIMWNDFRDIINKRLKGKVPEDKLLGPFFLKESELWNQNVFKNKLLYYLKE
DVFKHNPTIDFLNASTFSELIEKYDGSDNIFTFDIDDSSFVSD
>NpeUS61II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GATCGAC]
MNAVEIEQAISELAELPFDRAEFPFAFLEAFGNKATTLKKLRSGASNASDLLGGVLQRSNIHIATCDVGAVDQALKALRESPKTAAAKAKFILATDGEDF
QAEDLNTGETVACTFADFPNHFGLFLPLAGITTVKQIRESTFDIRATSRLNKLYVELLKDNPEWATAERRADMNHFMARLIFCFFAEDTDIFNGEGLFTR
TIEQMSARDSSNTHEVISQIFQAMDTPTRHEGKLDERYRKAAGTPRWADVFPYVNGQLFSGNTDVPRFSRIARAYLIQIGNLNWKKINPDIFGSMIQAVA
DDEERGALGMHYTSVPNILKVLNPLFLDDLRAALEDAADNGRKLLNLRNRMAHIRVFDPACGSGNFLVIAYKQMREIEAEINVRRGDANRKSAIPLTNFR
GIELRDFPAEIARLALIIAEFQCDVIYRGQKEALADFLPLDSQNWITNGNALRLDWLALCPPAGSGVRMQADDLFGTPLDQSEIDFENEGGETYICGNPP
YIGDKYQTKEQKDDLRTFKSGKQEVRAVDYIAGWFWKASDYIRHGGRFAFVSTNSICQGVQVPLIWPKIFGNGQAIFFAHENFMWGNNAARNAQVTCIIV
GVADAQERRKFIYSDAARKEVANINPYLSPGDTVIVARASEPISEVGVMFGGNIPRDQGNLLLSTDEMQALVNEYPQSKPLIRPIVGSAEFINGLRRYCL
WISDEQAELAFSIPPIAERLSKIREYRLNGSERGRAGLDTPYKFERTIIGNRHTIVVPSVSSERREYLPCGLMPPDVRVSNLALAIYDAPLWNLSVVASR
LHLVWVGAVCGKLETRYRYSNTMGWNTFPIPTTLTEQGKADLTRCAEDILLAREAHFPKTIAELYDPAKMPDNLRHAHERNDEVLERIYIGRKFRNDTER
LEKLFELYTKMTGKKAA
>S.Xor279I [EnzType=Type_I_specificity_subunit] [RecSeq=GAGNNNNNNCCT]
MAEHFHTLFTTESSIDALKQTLLQLAVMGKLVPQDPNDEPASELLKRIAEEKARLVVEGKINKQKPLAAFDEEAAPFELPAGWKWTSLAQVAVVNPRNAA
ADSLEISFVPMTFIGTRFDGQHAQETRLWAKVKQGFTHFAEGDVGVAKITPCFENSKACVFSNLVNGLGAGTTELHVVRPVCQTLDPRYVLAYLKSPQFL
LVGETKMTGTAGQKRLPKNFVETNPFPLPPLAEQHRIVAKVDELMALCDQLKARLGEARQVHGNLANALIGQALNGEKEPRAEAVYFSAYLISKLASRRT
FGRVAHMKLLFLADSHLGLGLMDGYRRHAAGPLDTSIYRVEERAAKEGLYSTSIEVLKSGQEKVSYHIGANISRAVETAASALGSARKELDRLIALFEGR
KTDDLEAVATLYAVWNDALAGGLHPSDEWLINEFRSNWHEAKERFTPDVLGRWLGWMRDNGLVPTGNSATTKSQAAFLFN
>CmaLM2II [EnzType=Type_II_restriction_enzyme] [RecSeq=GCNGC]
MANVIWNKFSEREKDEYIQFLKIFGALSGLFKDNQEGANARKPYLYYRNHEQLFARVFDVEDLTRKDSAFDALGTWANDRVGIGLKTWIHTKNYTYQKVA
EFNKLAPVVIAPLIENGEPEEVIKKVSELRNERIMLDKRLYRTDRDVYHFVTRDDDVMNIIETPYDLIDIDSLELIKSDGKTYTFKDRLHNYKFYKSKSV
LLEEFDASQCEIITQILIEQFDNPFELIRMIELPNSTKEIICEDDEEIYLPLYQDKKEGRIVSNCSGINIRHSKPKSKGSNTPRPEYEIEVRISKWIHHV
FPNFFGLNALNEEDIKNDQLNDFDLILPDGRILRGRIKQQNGKSLQTNPQGALGEWILNDVLGLKSREVVTWELLDNLGIDSLKIIKQDDKHFRITVAET
GAYEKFKIDNIENIVKAGLRGRQQPYFRPELVQELEE
>NspI [EnzType=Type_II_restriction_enzyme] [RecSeq=RCATGY]
MSKEQNLVQTIQSQFRQDSTQLQVFKLLSDQQWHCRECEGKKIGSNQYAGGGGIQGLQRGTRSRPGLVIETTKNYCQTCQQTRLGDRWTGEIKSANSASN
IPASLVEKILQVYSYTDVIEQRQREKHELVIDHRFPMERWGASEPPHLTSMNDNEIKRKFQLLKKDTSGNHNLLKSRSCERCIKTGKRGAPFGIHFWYQG
DENWPSVHQRGDEAEEGCVGCGWYNFEAWRNALNQKLSQSDQHK
>BstXI [EnzType=Type_II_restriction_enzyme] [RecSeq=CCANNNNNNTGG]
MAITLCDINGCRLERGHTGKHNKFPEFVWTSQFNKKDIDKVNKAGYATPRGGDKGAYQNHVYRNNKVIIPFERLENVNLNNYQDGYVIRLFPNQYFESAG
VVKPEFLQPNSFVKVGDNAFILYRTHSSFEELPPLPDWEVRHLKKNGNIVTRRSKDVIDAGHYVLRLSSISNKKERKEGPPQGIFAPEYANAETNYLSKA
FLAWLIIKTQNSPYNEEQFQHLRAILISHNLINISQLEEKAILKNGITCCPLCEQIIFYEQLHEMVSFEGASGLANSQEQVEGATRSTSVNLFHMVPLVY
ETLEHKPDQIAWGHAICNTRLGQRECLPLSRLKQEGTPVGLLDEDSNLEVLGWISKDKQFIRTENGEVWIKITDIEFNDDFEE
>TneDI [EnzType=Type_II_restriction_enzyme] [RecSeq=CGCG]
MRKTDPLIDIFKKNKERIDILGKAWDIFYFEGRAPDVGKRRENFIVEMIRKELSHFIKSVNQAPDTERNWDIEITFQDDSTKRYSIKTTEGFSDVKIAWD
GFPTKDRILTFNFQADIFYIARNKDKIILCVIDLEKLRDLQREVEKNESKLEEYYSLPSSNTNPRGFGLKSSTVKRLIELSKKEENYLEIDYKPFPVELI
LKAKEEYFKGWYDLIKELIEKYQQ
>DdeI [EnzType=Type_II_restriction_enzyme] [RecSeq=CTNAG]
MKAATDQELRKLIVLYNNVMEVMEHDAAKSMRDDNRAYGGFVRAAKGKIQELITERLVRTVWDVEMGENPERLSINSKKIKIPILRSYVDSINDENLKKY
ISSNILKYSYGLSVDKHVFIDNKFVLGIECKAYTENAMLKRILVDFYLLKTKFPKLNCFLFQLESQLGGDYSECNKFPIGSYPTRTIMSYFKNVDLNIVT
LLEGERKVDRPINKPQFFKPLKVEHLEVAIGYLQESLSEI
>M.RpaV [EnzType=Type_II_methyltransferase] [RecSeq=GCCCGCC]
MTSTNNYKKKLIEVAIPLEAINAASAREKSIRHGHPSTLHPWWARRPLAACRAVLFAQMVDDPSSDPERFSTDEAVENERQRLFKIIEDLVIWENSTNEE
VIERARVEIRKSCGGELPPIYDPFSGGGSIPLEAQRLGLPAFGSDLNPVAVMIGKAMIEIPPRYKDCRPVHRGVLDRNHYRNAEGLAEDVRFYGRQIREQ
ALERIGKLYPDVNLPKELGGGNATPMAWIWARTVASPDPSLQGQHVPLVNSFILSSKKGKEAIVKPIVDRALGAYSFTIERNALSKQDLETAALGTKAGK
AQDFVCVLSKNAITRDYIRTEGKAGRLGARLMAVVCEGKGGRTYISPSADMETLAQSAGEYADIATAKETFLSGSTPTRAMITGGVCSAYGLSTWGHLFS
DRQMVALLTFSELVHAIGAHIERDAIAAGLADDKIPLRSGGTGAKAYAEAVSVYLAFAVDKCADYWSSLCSWHSSGEKMRNTFGRQALPMVWDYAEANPF
CDSSGNWGAMLDWVWKTVENIHPGNSGVEVQSDAQAVIYPPNTVISTDPPYYDNIAYADLSDFFFCWMKPSLKHIYPDLFGLLSTPKAEELVATQFRHGT
KEAAEAFFLSGMRDVISNMAASTSNEYPATIYYAFKQSEIAQEGISSTGWATFLQAVVEAGYAVVGTWPLRTEMASRMIASGTNALANSVVLVCRKKSAV
AETVSRAEFIRALKRELPSAIGELQAANIAPADMPQSAIGPGMGVFSRYKAVIESDDSPMSVKAALQLINRELDEYLGGIQGEFDADTRFAITWFEQHGT
KTGEYGSANSIAQARGISVESVKHAGIVESSAGKVRILGRDELDADWDPAGDGHLTVWECCQHLIRALETDGEHSAATLLKKIGSEKADAVKDLAYCLYG
ICEKRSDAKDATSYNALIAVWTELTRQAAAIHDTGGDRQTRLDL
>Lmo23459II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=TAGRAG]
MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITRAKSKFDNGEPFVIKNKLHYEEIEGDVVIAIDAIAQSIKEQKSKPRYILVNDYLDIA
AIDTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDNPDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRT
AEDGSNFNEVIKELFAILDINELARNGKDEWLLEFPYVNGKLFGESHVDLTFSKVSRELLIEAGELLNWNEINPDILGAMIQSVASAEDRHVAGMHYTSV
PNIMKVIKPLFLDDLTESFETLKLRSEENELKDITEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLGDLQNNDSM
PLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELLPLKDAGNIVCANSLRVDWDQVTHFSKEDEIYIMGNPPYIGAKKQTPLQKE
ELESALDGNKKYKKIDYIAGWFYKGAKFIAKYNASLAFVTTNSISQGEQVAMIWPDIFKFAQIFFAYGSFKWSNSAKNNAGVTVAIIGLTSKTEHKDKFL
FSEDIKKKVSSIGPYLAEGDEIIVATANSSIAGFPNAYLGSLPLDGGNFIFDETDRNDFIQKYPALSNLVKKYIGSSELIKQEYRYVLWMNEEEYIEHKD
NPVISERVEKVKHFRKNGGQSAQGAVDRSYEFFTKNQREQAISNNRKHSKKEMKTIIIPRVSSENRSVVPMGIVGEDTVVSDSATAVYNAPMWLLGLLES
RMHMVWLRSIGGKLKTDYRYSAGLVYNTFPIQNLSTQRKNEIARVMTEILDLREYEGGTLADLYNKDSMPESLRKKHEELDGIVDRAYQQRPFESDEDRL
GTLLKLYQEMTTND
>Mae7806III [EnzType=Type_II_restriction_enzyme] [RecSeq=GGWCC]
MSYLYSYRHNLTQLLEQINLQKPSIKIPTFVTHDLVDTYQICRLIDDFIFEYFQENRTTDTDIADNRDQKIDDALDEFQSKVVEKILKEKQDFKNISLKK
KKGFKNIFEFAQCENLYLSNKYVNLISESLGHTLEEIASISSQVFVPEKILNFKIKGVDLVVFNQGIIKYTQLKTKKDTLTGSQSDRSINELKIHPNSVF
AAALDMGNSWTISKTKAKENNIELLAGQAFWSMLDLDYETILNKLKMTVRKIEKELYQV
>SuaI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGCC]
MDDPDMKFYGKIEGIRYKASFKMPVLHEYSLLKEGIENQTAFIYKLGSHKFAVSWWVSPKRTRSYPYARVYNTLQFSGKKVTIIPVMKDEGEEGDRDFIQ
WDTISLMSLLGVYVILGYYVEASKKSQGKITSQRFDYEYLKAQFEELSNWQSDALHWNINQVNKIGDLMNMAIEAYERISQRLNVRLKDIQSAKNKAEEV
YRDLEKFKNHSRTQSEKAQQREVLTTQPKENITGEKAPIDIENYLGGLYHFTIDEAYVNLQSKTACIVEAKNSKSNCLPSEEDIKDGLLKMIIFLNLSEL
YYEYGDAYIRIEHINPFLKLTGVCESFKPRHKVVLEELLREAKENNLKIILNQKVIDVKGSEILYYTDFMC
>BspRI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGCC]
MAQRKYGALEQKVANIFINEQVFTFKGKRYRVLKIGKPQKQGGSGEPKTDVYILGQELGGTDTVELKISVKKRDFEFIENKITPIRMADILGANWQKIIQ
ESSERIKDRFADCSLIYPERRGTTLKNSITLGWKAEITNRPRTLSARMQLSGQQIKDLLYKGVNLPPEKRDAIVDGNIIANSGIAEYILETDLDDIHTTA
DILSQMELIDDANLNEAYLAFTANNYRTDTDKCDGARSLAVRVKWEIINGKLAHHIMFGQPLLYTGQAAVPELKQTLIQLGTIHPENMNDNSILDPSIVC
KKKR
>RpaBI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CCCGCAG]
MGTASIPDVEQFISRWQGREGGQERANYVSFLNELIALLGLPKPDPADATHEHNDYVFERAVKKHKDDGASHGRIDLYKKNSFVLEAKQSRLKGVKKVAG
QNDLFNADVPEASRGRRGADRAWDVLMLNAKRQAEEYARALPASHGWPPFILVCDVGHCIEVYADFSGQGKNYTQFPDRQSFRIYLEDLRDETVRERLQK
IWTEPQALDPAQASAKVTRDIAKRLAQVSLALEKKGFVADDVAMFLMRCLFTMFAEDVGLLPDKSFKTVLEECEKNPEAFVHDVGQLWEAMDLGAWAHAL
KTKVKKFNGEFFKNRAALPLGREEIGELRQAASYDWNEVDPSIFGTLLEQALDPQDRKKLGAHYTPRAYVERLVVATIIEPLREDWRNVQATAETRRGAG
DLKGAAAAVQAFHDKLCATRVLDPACGTGNFLYVSLELMKRLEGEVLEALLDLGGQEALRGLGSHSVDPRQFLGLEINPRAAAIAELVLWIGYLQWHFRT
KGGPPDEPILKAFKNIQVKNAVLTWDGAPLPKIVDGKETYPNAKRPEWPAAEFIVGNPPFTAGQDFRREFGESYAQALWLNYKHISGAADLVMYWWDRAA
ELLNRKGTALRRFGFVTTRTITHEFSGRVVARHLNANDPISIVLAVPNHPWTRGRDAAAVRIAMTVVEAGHRNGLLQNVISESDLDADEPTILLEDRCGK
INPNLTIGIDLASAGRLRATDGICHDGVKLHGKGFIVQSAELEHLGLDRRKGIEKVIRPYLNGRDINQRTRGLFVIDLFGLAEDEVRLNFPEIYQKLLLK
VKPDRDANPRPSRRDNWWLFGENQPKMRNAIASLGRYIGTVDTSRHRIFSFLDEKMLCDDKVVIVASTDAFDLGVLSSRIHCTWSNKSGVRLGVGNDPVY
ASNRCFDPFPFPAANNIQKQTIRVIAEELDAHRKRVLKEHPHLTLTGLYNVLERLRAGAAIAPSSASAARGAGGAGGDAGAVARGKTPHPNPPHRSAGGG
DQAAAAASAVPALTPDEQRIFDDGLVLILKELHDKLDVAVAEAYGWPADLSDDEILAKLVALNKERAEEEKRGLVRWLRPDYQIPRFGKGLDKQAAREEG
AQVTADLIGVETVQKRAFPTVAVEQTAAVFAALAAASAPLDAKTLAAQFKRTKTTEKKVGEVLASLARLGYVTSDDGMTFALRRVA
>NheI [EnzType=Type_II_restriction_enzyme] [RecSeq=GCTAGC]
MSSYHDDLNILNVDFNHLRLTELIKLADQAEPFYLWVEKIFRQVSGRADSLETIIEVEERVVLKMAILTCFTSDEKELPKLFNGVGVPYPHIKACYFFFA
WLVRDAATQRLDPLIREAFTQLKSIHPQMKKTELESEIFSQLLVNYRNELIHFSWPVIREVLISRLEGSRRAARGSYLELFVRTALAQSITYFYKIYGNY
GKFLDVKIHDKPLKVKNRTYDVVAELIGNNHNTQYLILPVKTRETQGGGHAHLFTRDIEQSNNDIRELYPNAVIAPVIIAENWSDTEKDLENVGYNDIFH
FSVNPNRFAGFSDVEQIRLNRLVERILL
>BbuB31II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] 
MNNNESLFKKAKEYIANLKSTKLEEKTEYSNRTHLENLLNDFNKINQNSSIAIQHEPRRSKEGFGSPDYIVRHNITQGTIGCIEVKKVEQNLDEILKSSQ
IEKYKNITRSILLTNYIEFIWIKDKEIKLRGSLLTKEELYSEDTKLDKNKLTKIINILFEFFNSHFEKIKSIEALASLLASKTKSLKEEIEVNLKENKEK
LNLDIDESELGELNVLVSTCRILKKSIYSDDFNISEFSDSIAQTITYGLFIARLNSKENIEINFKNIENFIPTNFSLIQNIIKLIKDIHKDSEFNYLRWI
LESIISIVNNIDTKLIFNEFSFTNSSLNLKDPYLYFYEDFLAKYDVSLRKAKGVYYTPSPIVSFIVSSLNEMLKKEFKLNHGLANKEKVTVLDFATGTGT
FLLEVIRTIILKEIPEESGRQKDYINLHILKNLYGFEYLMAPYAVTHLKLSQYLKEVCKVDFNKDSKLKVYLTNTLDLKEITDQKFFSFSFFKDIAKETK
EANEIKRNPILVILGNPPYSAESKNNNQYILNLVNDYKKIKNSPINERNTKTLNDDYVKFIRFAENKLESNKKEGLLTIKGSEEGLLGIITNNGYLDNIT
FRGMRHHLLSTFDEIYILNLHGSSRKKEKTDDGSIDENVFDIQTGVAIAIFAKYKEKEKKNELANVYYSSIKGKRDHKYDFLNKNHIYDLNFEKLDYKEP
NYFFLKKDLSNEDIYNKGKSLIDIFNEFNVGIVTRKDKIAIDYTKDNLMDKLRDFAYLPEQDARNKYDIEKDSMFWKLSEIQKFLKNTNFNKSYVKEISY
RPFDNRFTYYSKNKGVVVEPRYKTMKHILEIEGNIGLVTTRLLSTNSFKHALVTSEISDKCFVSNKGSESCYLFPLYIKENQGVFKNIKKENFKKDEKDN
KDIFREFINTKYSKIFTPEEIFGYIYAVLYSNTYREKFYEFLKIDYPKIIFVNNVDAFLELSKLGTKLINSHLLKDNLKLNNSIGNHTGDYNRIVEKITY
KEDTKELYYNSTCCFTNVAKEVYEYKIGSYQVLKSYLKYRKGKELIINENEDEIEHLEKVIKVLSYTINVQKEIDEIIQNLKEFNE
>MboII [EnzType=Type_II_restriction_enzyme] [RecSeq=GAAGA]
MKNYVSNINLGNSSLKFIDERLQSENYRGIHLSQHNRYDLPKLIDILTLLNKHAPNQSLMQIRTTDISKRPQNIPEEQSYAEFCNEAKSLTNIGTQDAMR
KNLFVDFARMGLINRYNDKKVLTDPFKRGVTKYVALSDMGVKLIDPKLDILSKNLIFSKSLNKLLTGFVEDVLSLLTNSDLKEISFDEFMLFVSAMNCNF
NFSISTEQCESLIKEYRLLSRVQKNAVIDTLKSELIPDNFNGDKKDKRDYHNWANENQQIWTLFENIPFFIMEKDSRKLILITSDVDLSKYSKSKMKRSQ
QAKNDYFKHHKVNKIKGYELDHIIPLLEAESVDEYRYLDNWLNLLYIDGKTHAIKSQSGSKYYIFTFDDNDYNQIYFLDTQGDKLSINNDDTALFDKNKV
PKIYEYNQNFINAKTS
>Bsp3004IV [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CCGCAT]
MTDAERREAARQFYQKWVNRGKEDEDDRSYWIDFLQDIMGVDHVTDRIDFQKKVVGPDGNTKRIDAYIPETRVLIEQKSLNIDLSKPQQGHNGMTPYEQA
KMYDNSLPVSEKAKWIILSNFAEIWVYDMDTRVPEPTKITLAEIPTKYSLFEFLINQKQQKISQEMEVSVKAGEFVGLIYDALLKQYINPESEESLKSLN
MLCVRLVFCLYAEDAHIFGENGHMFHDYMEQFETKDMRKALKELFKILDTPESERDPYDISDLSKFPYVNGGLFADTDIEIPNFTDEIRDILLEKASEGF
DWSMISPTIFGAVFESTLNPDTRRSGGMHYTSIENIHKVIDPLFLDDLKSELKEIKEIQVPKSRNPKLKAFQNKLASLTWLDPACGSGNFLTETYISIRR
LENEVVQTLKGGQMQFVFDEDFSPIKVGIDQFYGIEINDFAVTVAKTALWIAESQMMKETEDILLMHLDFLPLKTNANIHEGNAIRIDWESIIAPSKLSY
IMGNPPFVANSGRVSAGDASSKGMQNSAQKEDKQLLFGKNGGVLDYVACWFMKAAIYMKNTSIKAAFVATDSICQGQQVVPLWKPLFEMGIHINYAYEFF
KWESEASGTATVYVVIVGFSYKEIPCYLFTSENEKILVKHINAYLVSADDVFINKRSKPLFDVPEMLNGGKPTEGGFLILTSEERTALLAAEPQAEEFLR
PYMMGKDFIDRTPRYCLWLVNTDPSKLRTCKKVLERVENVKQYRLKSPKAATRKKAETPMLFDEVRECKTDYIAIPKVSSSKRRYVPIEYLSREIIPGDK
LFMIPDASLYHFGIITSNVHMAWMRATCGYYGPSYQYSNTIVYNNFPWPTPSEAQKQKITETAQVILNIRNAYPNSTLADLYDPLTMPPELQKAHTANDK
AVMQAYGFSIKDTSEADCVAALMKMYQELTKE
>BsrDI_(BsrDIB) [EnzType=Type_II_restriction_enzyme] [RecSeq=GCAATG]
MTEYDLHLYADSFHEGHWCCENLAKIAQSDGGKHQIDYLQGFIPRHSLIFSDLIINITVFGSYKSWKHLPKQIKDLLFWGKPDFIAYDPKNDKILFAVEE
TGAVPTGNQALQRCERIYGSARKQIPFWYLLSEFGQHKDGGTRRDSIWPTIMGLKLTQLVKTPSIILHYSDINNPEDYNSGNGLKFLFKSLLQIIINYCT
LKNPLKGMLELLSIQYENMLEFIKSQWKEQIDFLPGEEILNTKTKELARMYASLAIGQTVKIPEELFNWPRTDKVNFKSPQGLIKYDELCYQLEKAVGSK
KAYCLSNNAGAKPQKLESLKEWINSQKKLFDKAPKLTPPAEFNMKLDAFPVTSNNNYYVTTSKNILYLFDYWKDLRIAIETAFPRLKGKLPTDIDEKPAL
IYICNSVKPGRLFGDPFTGQLSAFSTIFGKKNIDMPRIVVAYYPHQIYSQALPKNNKSNKGITLKKELTDFLIFHGGVVVKLNEGKAY
>TsoI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=TARCCA]
MSPSREEVVAHYADRLHQVLQKTIAQNPNEAEFRRAVEPLLEEFLREMGLEPLARAEYTLAQGRADAIFNRLVIEYERPGVLKPKPDAATRHAVQQVKDY
LSGIAQRERHAKERLAGVAFDGRYLIFVRHMGERWVEEPPVEANPHSLKRFLTWLAGLASGIALTSENLNRDFSIEQLRTQTILRGLYQALEKALAEEGL
VRQLFEQWRIFFSEAIDYSETFGGRKLEPLKKWVRKAGLHIQTPEEAERFFFVLHTYFALLAKLLAWLALSRHMGVRLGAPVFSALAAADGETLQKRLGE
MESGGIFRQYGILNLLEGDFFAWYLHAWSSEVERALRALIERLDEYDPTTLSLFPEETRDLFKKLYHYLLPREIRHNLGEYYTPDWLAWRLLVQLDNTFF
AGTPSPNDEKLRQKLLSTRFLDPACGSGTFPVLVIGRMLELGRLLMVPERDLLEAILKNVVGFDLNPLAVLTARVNYLLAISDLLQYRQGDITIPIYLAD
SVRTPAEGQDLFSQGIFVFPTAVGDFQVPAVLVTAPKRFDRFCEILESSIRSEVDPQAFLERTRRELDLNPSEWDDNARKLAEELYTKLLDLHRRGLNGL
WARLLKNNFAPLTVGQFDYIVGNPPWVNWEHLPDNYRRSIAPLWARYEIFSHKGFDAILGKSKDDISVLMTYTVMDKLLKDKGRLGFVITQSVFKTGGGG
QGFRRFRIPQGKEQFTPLAVIHVDDMVDLNPFEGASNRTAVMVLEKGKPTKYPVPYTVWRKKRGTRFTYDSTLDEVLSATRRLHFFAEPVDPKDPTSPWL
TARPKVLKAVRKILGKSDYRARKGVTPSVNSVFWLIEALKRPDGLILIHNLTEGAKVEVGEVSETIEPDLLYPLLRGRDVKRWRAEPSAMILITHEPGMR
LKAIPEKEMQTRYPRTYGYLKRFEQVLRRSAVFRRYFIRKKGGELVDAGPFYSMFNVGDYTFAPWKVVWREIASDLTAAVVSSREGKTIVPDHKLVLVAC
SSDIEAHFICALLNSSIVRFVALGYAIQTQFAPHLLDFIRIPRYNPTDPLHRRLSELSQAAHKAAQAGDEKRLEALEAEIDREAAKLWGLTEAELREIQE
SLRELEGEVPAAEEEA
>SfeI [EnzType=Type_II_restriction_enzyme] [RecSeq=CTRYAG]
MNIDQVANKMKRDLELAITDQIVDGSKVNKKGKLFLNGAEAKQSLIRSSKLINYVHEFVKHELIRNNVEESLIFPPLGQTKPEIKLTGMFKQKDQDVCVK
PQGVLPERTLIEWGPMINSGLYCDYGGIYSERVLSINVRSQLSSLDKNSDTLFERMFAEALNLHELYPKIVMGEVYVIPVYEYDDQAMINNQVKFKSRRT
NLEKYINFFYYLSGRDEQDLEEDKQKYERCALVIIDFRGDQAKVYKNTAELKARGLVRNDFEVELAELSTDKFIEDLLLIYNNRFPKRNEGNL
>M.MscI [EnzType=Type_II_methyltransferase] [RecSeq=TGGCCA]
MPFVIAVHRESLIYADPLHIDSVPRDYAKEVDVAHHVELLKLLDAHQGPAVLSGYANDLYDTTLAHWDRVVMKAPKVEKGASRAEVLWIKQKE
>ClaI [EnzType=Type_II_restriction_enzyme] [RecSeq=ATCGAT]
MTYLILRRQQRMKNSAQMIKDNIMKEQLTIYHEIEVGDPEFWYSTEQMEELLNEALQGTDLNGMALRTRSKFVKVKICEAFGYQVPKSFKKTQPRFLSQK
FDVYNQKSNNLQIWNEEISPSRRYVLIKISFDDIITQVKVVTGDVLATLDSTGTLTQKYQAKYAGVHERKATLLSECDTDFIQSITQSYNSFDEFTAPDT
NPKEDELMGIDEIFDKLKDLIGTKIPYIGATQERNRGGHLHKMICDALGYNNFKENGQFPDIKHQLLEVKLQTSETIDLGLFTPNSYELLDIPQLNNESI
SMLDVRYAIFYGDVIEDTITITHFYLVTGEDFFTYFKPFGGKGINKKIQIPLNEEFWNL
>M1.BsaI [EnzType=Type_II_methyltransferase] [RecSeq=GGTCTC]
MSNAKSFSLNEKTEANALIDFIIEKSNQSKDLGYWLQKSKGQFYTHNFIGEKLVTEIVENIKFNDDSEVIKIIDPFCGDGRLICILLDKFNAINKFRNTL
LEIEFWDIDPEAVEVAYTNIKEKANALEFNVQLKGRVCDTFLFAQDYFGSYDICITNPPWVIIKPDKKEKERLSKEEEIEYIEILKNFDDFLSRYYPTSL
PTKKYGGWGTNLARCGTEVALRLISKVGICGIVSPASLLGDQVSDNLRVWMFNNYEVYSISYFVAEAKLFGKVDQAVITLTLSPKCDDSSGDIIPHLFYY
DRELFEKRYYMDEYDWRIIKSLNYVIPIQFGLEIIKMNRLFKSLPTLGDLENEKEGIWLGRELDETGIKEKLANKGQYRFIKGKMVGRYNLIEESNQYID
VRKIDKIPKSVEFYRLVWRDVSRTTQKRRLISTIIPPKYITGNSLNVAYFKDNNLKKLKALLAIMNSFVFEAQVRANLSTNHISLGIIRRAHIPKLEGRV
VDELSQLVDNYVNEESELLLEVKVAKAYGLSFEDFSSILSLFDKIGKDEKEKILQVAKKYLKGGIKNDS
>M.Mtu70007I [EnzType=Type_II_methyltransferase] 
MQPSHPTRPGAVIRYVGSSPDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRD
FASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFL
LSRYREITLVTFERLVFDGVLQEVVLFCGVVGPGPAHIRTVRLGDANDLSALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGEL
ADVDVGIVTGRNSFFTFTDAKAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWW
STPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDV
DLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>M.NspV [EnzType=Type_II_methyltransferase] [RecSeq=TTCGAA]
MIHNVEKTKLEYGDFQTPLELAEKVCEKLVQLGVNPDIIVEPTCGLGNFIEAASYSFTRAKKIFGIEINSNYLDKIKEKQIFLNDEKFDIRCGDFFQVDW
SSIIGQLNKEILIIGNLPWVTNSQQGSIDGENLPIKNNFQNYHGLDAITGKSNFDISEWMLIRLVQCLQKHNGYLAMLCKTSVSRKILNYIHSQKLNLAY
SATYKIDTRKYFRVNVDACLFLCKFDLISKNYFCDIFDNIETSKYYRIGYHNNVLIKDLVALKRLKNLYTNKSNIKWRSGIKHDCASVMELQKINDTFIN
GLGEIVDIEETYLLGLIKGSYVAQNKINATDRYILVTQKIIGEPTDNIRDLAPKTWQYLEKHEKLLDTRKSKIYQNKPRFSIFGVGSYSFSPWKIAICGL
YKKLEFRLVGKISEKPTIFDDTFTFSVLRMKKSPLKPLRF
>M.MtuH37RvI [EnzType=Type_II_methyltransferase] 
MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRD
FASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFL
LSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGEL
ADVDVGIVTGRNSFFTFTDAKAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWW
STPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDV
DLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>Spn23FI [EnzType=Type_II_restriction_enzyme] [RecSeq=GATC]
MDQNLFNYNDEDIDSVIEYSHKLLNRKFSDVMEEYNRSLYKSYDDYNDRVVSEVQDKAISMKSKGQYGNYIEKYFYGYQPNSDSEADFEKIGVELKVTPF
KINKNGTLSAKERLVLTILNYMEENLEDFYSTHLWKKCAKILLLFYNGLIPNQTMKDYVIEKIFLYEWFEEDMAVILEDYQKITDKIKNGKAHELSESDG
NYLSTCTKGAGKGKDLRQQPFSHELAKQRAWELKSSYMTYLINHKIFNQSDQESVLANFRGEKKSFTEVIAEKILSYKGFSEQELYDRFEVNSKAKGKNS
TLIRKILGLTGDLDKTKEFQKANMNLRVIRVDKNNLPKEDSPFKTYCFKELAATDSWESSHVYNEIYNKRFLFVIFKEIEPKLFVLDSIKFWGFQDRQLE
EIQRVWQETRQIISDGVKLTQNGNKVSTNFPQSKINKILFTKLHATNTYYEIDKGKFVGKGSLSDTDELPDGRRITKHSFWMPKKFIKEILDGNWD
>M.Hpy66VIII [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MVSNTILQKNLDAFYTHPKIARFCLDLLKDLINQNLGLGLNAFHFLEPSAGSGSFVGVLKGLGIADCLAFDIAPKAQGIQKKDYLLELIEFNKKCIIIGN
PPFGYRGKLALKFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIAPPKIRHNDFI
TYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALMIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>TmaI [EnzType=Type_II_restriction_enzyme] [RecSeq=CGCG]
MRKTDPLIDIFKKNKERIDILGKAWDIFYFEGRAPDVGKRRENFIVEMIRKELSHFIKSVNQAPDTERNWDIEITFQDDSTKRYSIKTTEGFSDVKIAWD
GFPTKDRILTFNFQADIFYIARNKDKIILCVIDLEKLRDLQREVEKNESKLEEYYSLPSSNTNPRGFGLKSSTVKRLIELSKKEENYLEIDYKPFPVELI
LKAKEEYFKGWYDLIKELIEKYQQ
>M.LmoA118I [EnzType=Type_II_methyltransferase] 
MKILDACCGSRMFWFNRTNKNVTFMDNRELETELCDGRKLVVKPDVVADFRSMPFDANTFHLVVFDPPHLLKAGDKSWLAKKYGKLDQRTWKDDIKKGFS
ECMRVLKPNGTLIFKWNEDQIKLSEILKIIDFEPLFGNKRSKTHWLVFMKEEQA
>BsuJPCI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GACGAG]
MALIDLEDKIAEIVNREDHSDFLYELLGVYDVPRATITRLKKGNQNLTKRVGEVHLKNKVWFKEAKKGKLFDALIDIEQQVEYLSAKPRYLLVTDYDGVL
AKDTKTLEALDVKFEELPQYFDFFLAWKGIEKVEFEKENPADIKAAERFARIYDVLRKENNIIETNRGLDLFLIRLLFCFFAEDTDIFKRNSFTNLIKTL
TEEDGSNLNKLFADLFIVLDKNERDDVPSYLKEFPYVNGQLFTEPHTELEFSAKSRKLIIECGELLNWAKINPDIFGSMIQAVASEESRSYLGMHYTSVP
NIMKVIKPLFLDKLNQSFLDAYDDYTKLENLLTRIGKIKFFDPACGSGNFLIITYKELRRMEINIIKRLQELLGEYLYVPSVTLSQFYGIEIEDFAHDVA
KLSLWIAEHQMNEELKNEVHNAVRPTLPLHTAGDIRCANAIRVEWTEVCPAQGSEEVYVFGNPPYLGSKKQNKEHKSDMLSIFGKVKNGKMLDYISAWFY
FGAKYASTTNAKVAFVSTNSVTQGEQVSILWNELFKFGIQINFAYKSFKWANNAKNNAAVIVVIVGFGPLDTKVNKYLFVDETKKLVSNISPYLTDGENI
LVSSRTKPISDLPKLHFGNMPNDGGGLLFTITEYTDAINKYPELVPYFKKFIGSVEFINGGLRYCLWLNEAKYEKIKSNPLIQERISISKNHREKSTDKG
TNKLALTPWKFRDTHETTNYSIVVPSVSSENRFYIPMGLAGADTILSNLIYVIYDAEIYLLGILMSRMHMTWVKAVAGRLKTDYRYSAGLCYNTFPIPEL
STRRKNEIEEAILEILDLREEQGGTLAELYNPSTMPIELKVAHEKLDGIVERAYRQKQFESDEERLEVLLKLYQEMTER
>S.SspMR4yII [EnzType=Type_I_specificity_subunit] [RecSeq=CGACNNNNCTG]
MRKRLDADFFAHPETERLEAIERDPRALRIEDICLKKTSGFTPKLPNLKDGEAKFITIDCISSLRLHEERFKNIPLSAYEAGFSKTRRIAAGSVLCTIKR
RICQAYPFIENPKENLAFNQDIALLVPNDERVSAVFLAAYLGSSIGQALANREQTEQMNPYLSLIGLGNLPIVPVGKAVDEGISRLFFESNSKSDTARQK
RNEAEDLLLDALGLREWSPPEPLAFTASANEVREVERFDANYFAPKYLTVLEKLISTNAAHLLGDGLTKLVSRGQQPKYGIVGLPVINSKHVRVNRVLLS
DANRVAQPGKHIIQKGDVLINGTGVGTIGRAAPYLEDQQAVPDNHVTIVRPIGIDPLYLSVFLNSPVGQMQVDRMISGSSGQIELYPDDVKQILVWNAPE
ALQIEIAEAIQQSFELEGRANQLLVDAKRAVEIAIEGSEAAALAYIAEQETAYA
>ApeKI [EnzType=Type_II_restriction_enzyme] [RecSeq=GCWGC]
MNQQTCTSAIVRAVNSGKLPREYLLYEDIKRRTRHVDTLCEGALRGRDIEAFSSLDEWIDWLRWSTVVLDENDYLEAAVHALDLAPRLAGTDYGMSRQRD
LGQLWTDTIRGFLGELAFVKWLEREYGIKAMLDYRKGPLEEFLPSDIKEVEKDGVRRPPNLKVSIKTTKLRGIWLDVPYRQVDHSDVFVLVRVGVTRMHF
LAFLKRISAIRDKMLSRALELGVITEGEAESIWDLIPDFTGIPAYIAGFFDKREYGSAIGRRTAIFLAEGVMKRKKFVINKFIGYWNPKEDVYKNTVVEH
LQRLGVIPSRRDVKIEFEGIGEFTKTLHFLVSSGALRRKREEWERIISQL
>S.Eco3609I [EnzType=Type_I_specificity_subunit] [RecSeq=GNAGNNNNRTDCA]
MYSRVNSNLLEDRLDAEFYSLEQIKNHKTLQDYGVIPLSAYCNGINVGYTGELTSVYRAQGTTLFRVSDIDGIFLSNDDVNYVPDDFAANNPQIWIRDND
IVFAAVGNTIGKVALKTVSMPDGVCSRALMIARPLTDKIDSSFLIAYLATKYAQKSLLRGVSGSAQPVLNTPLIANLPVISAESVSQKYIGDKVRQAEQL
RVWAKRLYRIPAFIDAFIQGALPLSLTDLLFSLIKKSNNDAIKELTKFLALNIEDAQMKQNFQGFIEKNKMGSVDIEREFFHINTVSTSAIEDFLSAQTY
RPAITDAFDKITSKKFSTLQSISLEPIRQGATPKFSAIGKKCIKSKQTRDLFLDETGYEIVDPNDEQNKRIVRLKRNDILVTRQGAGTVGRASIFLDDEE
TYITDSLFLVRIDPAKANPAFVAGFLRSYTGQRLIEKGVYGSTGQLNLSSTALRNIPILDVPLEFQNFLGETLCTADTLFKLSTRLTQSAKTLVEALIEG
QLTEQQLIQAQQALEDGDNSLDQAILSKLSAEGYAIEGATPLFSDVDELYSLLEEAAQAEAEE
>TliI [EnzType=Type_II_restriction_enzyme] [RecSeq=CTCGAG]
METSVCHTLKSPVIKKFCESITELARTSRGYFEPIQDDFLKAYYQIVEKARINGRLPEGEYRQKGNAFRDFISELIYIRSGGIYRLTDRRIPGYSERTHD
VDLAYVRDATVLVAGEVKMTGSPRHKKGTTVQKERKTQSDLDKRLKEVKFTAVDLKLRYTPEEAIINALNSKNTFSEVSNNSWWMRWIHTSIPGFYSFWA
SRLASGRLDKKTGRRVDFDNPDLLLEKFRNLLKYNNAVGLFMFREENGRYVPVETERIKRERISIDDAVKDLIKFLDTHLD
>Bsp460III [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CGCGCAG]
MGETDLDVDTFIERWSQARGGAERANYQMFLTELCEALGLPRPDPASDDTRTNDYVFERGVKRRESEGLASTLRIDLYKRGCFILEAKQSRAAHRDDGQG
TLFKADETASTAASQGKWDVLMRNARRQAEDYVFRLPADHAAPPFIIVCDVGYVFEVYADFSGSGRAYSHFPDRKGFRVRLEDLRDAKIVKRLKAIWTEP
LSLDPTRESARVTRQIAERLAEVSKRLEKKHPAEEVAHFLMRCIFTMFAEDVDLLPRGRFTALLKDQLDHPESFAPMLQSLWKSMDAPEYENRFYAGFGL
HLRHFNGSLFKNAKAFPLGREEIGELLAASSHDWRYVEPAIFGTLVEQALEPGERRKLGAHYTPRSYVERLVEVTVMEPLRADWNAALIKAGDARERGDA
AHSSSAERDSAKREAVAAIRAFHHQLCMTRVLDPACGTGNFLYVSLELMKRLEGEVLEALAELGETEGIGLETVDPHQFLGIELNVRAAAIAELVLWIGY
LQQHYRNSVEHPAEPILKAFGNIQQKDAVLTWDGYPEHQFEMKDGVAVQVYPNARRPDWPTAEFIVGNPPFIAASDFRKAFGSPYAETLWRVHSHVNGSA
DFVMYWWDRAAELLTRKKSQVRRFGFVTTNSISQVFLRRVVERHLSAKPGLSIIFAVPDHPWVKGTPGAAAVRIAMTVVAQGRNDGVLSEVIGEAGLDTD
DPKLDFRALRGRINPDLSVGADVTTAAKLSAGAGMAHDGVKLHGAGFLLRQGEAEHLGLGTRQGLEQYIRPYRNGRDLTAYSRDLKVIDLFGVSAEEVRR
AYPEVYQHLLDTVKPERDRNNRASYRDNWWVFGEPRRDLRPALAGIERMIATVDTARHRLFQFVASKVVLDDKSVVIAHDDGATLSILSSKIHVTWALRA
GGWLGQGNDSVYTKSRTFDPFPFPDPSDALRTILSDLGEELDATRKTVQAEHPDLTLTGLYNVLEKIRAGTPLDAKDQDVKERGRVLILKDLHDQIDRAT
ADAYGWPHDLSDEQILERLVALNAARAAEEAAGHVRWLRPDYQIPRFAKGAAAKSGRLDLSETVVAIDKSLPAFPKDKGEQAMAIHAVLEAAHRPMDAAA
VSRAFKGGGKRIEQRVVQALSTLVRYGWITALPGGTYAARRAA
>MchCM4I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAGGAG]
MRLSWNEIRARAARFADEWKDARYEKGETQSFYNELFEVFGVKRRRVASFEEPVKLLGTRRGFIDLFWKGVLLVEQKSAGRSLVPAKSQALDYFPGLKDT
ELPRYILLSDFQTFELYDLDEGTEVRFPLSDLPRHIEAFGFIIGVQRRTFRDQDPVNIKASELMGKLHDALKASGYVGHDLERLLVRLMFCLFADDTGIF
ERGIFETLVRERTREDGSDVGPWLQHLFDVLNTHEDRRQTALDDDLKLFPYVNGELFAERLPIPAFNAEMRARLVEVCEFSWDAISPAIFGSLFQSVMNA
KERRAAGAHYTTEKNILKVIEPLFLDDLRAEFERLRVRRDTGRGNALRAFQDKLGGFRFFDPACGCGNFLIIAYRELRALETDVLKELNPSGQRTLLDVA
TLSKVDVGQFYGIEIGEFPARIAEVALWMMDHIMNNRLSLEFGQAYARIPLKSSPHISCGDALEVDWTKVLPPAECSFVFGNPPFVGAKYQTDLQRGQVR
RIAQLGGSGGTLDFVTSWFLKAGAYVRQAKAPIGFVSTNSIIQGEQVAQLWPLLFQRYGLEIAFAHRTFAWGSDARGVAHVHVVIIGLTARDQQPATKRL
FSYERITGDPVESRHGALTAYLFDAGQVENTHLVVEECSQPLGDVPRLIIGSKPIDGGHYIFEAAERAAFLAAEPGAARFMRPYVGSEEYINGRQRWILA
LADASPSELRALPAVMERVAEVRRKRLASPSKPTRLLAETPTRFHVTVLPQRPFLVIPKVSSERREYVPIGWLEPPTVPSDLVFVLQDADLWHFGVLTSR
MHMAWLRHIGGRLKSDYRYSAGIVYNAFPWPTADEGQRRKVVDLAQAVLDARAVFSEASLADLYDIDAMPPELKKAHRALDAAVDRLYRGAAFGSDRERV
EHLFGLYEKLTMPLATVAKPRARVSRTPKPVAAPVHQ
>M.Pbo13188I [EnzType=Type_II_methyltransferase] [RecSeq=CTCAAT]
MALVPFEWKDKPALIEHLFPVQKLSAESYKEQMANTGKALTALGSYWKGRKPLILSKACILGALLPATEDRLQDLEIFELLMGMDSDSMQKRLEEKLPAS
KRDTVGELLVLPYNEQVKAAKRPDEIGDSLSIHIWTKVNQYLGTSAASFPELIEQIGTARYGRRPKVADVFCGSGQIPFEAARLGCDVYASDLNPIACML
TWGGYNIVGASEEKRIKIDNDQEHLARKVRKELEELGVDSDGNGWTAKAYLYCNEVVCPESGWKVPLLPTKVISQDYMAVAELIPLPDEKRYKIDIRFVN
SEEDIKNAKVGTVQGADLVHSPDGANIYRVSLKSIRGDYKDSRDTKNKLRLWSKTDFIPLPGDIYQERLYCVQWQNKIDGKNQVQIRPVTPGDEEREQKV
INYVREHLEEWQDKGYIPDMVIEKGYNTDQPIRERGWTHWHHLFNPRQLMVNGLINKYCDAYLAPNFAQLLNYNSRLCVWHSAKGQGAGSISNVFYNQAL
NTLYNYGCRSLELALKKMADPIKNEPLTNTVLFRSEAHPAQELEVENDIYVTDPPYGDAVKYEEITEFFIAWLRKNPPKEFAHWTWDSRRSLAIKGEDEG
FRQGMVAAYRKMAQKMPDNGIQILMFTHQSGTIWADMANIIWASGLQVTAAWYVVTETDSALRQGANVKGTIILVLRKRHQELETFRDDLGWEIEDAVKE
QVESLIGLDTRVRAQGSEGLYTDADLQMAGYAAALKVLTAYSRIDGKQMVIEAEAPRQKGKKTFVDDLIDFSVQTAVQFLVPVGFEKAEWQKLQPVERFY
LKMVEMEHQGVKTLDNYQNFAKAFKVRYFEQLLSDSSKANFVRLKLSTEFKSTLMNENAEIGRTPLRALLYSLFELSKEVEVDDVLLHLMENCPNYLNNK
ALLAKMADYLAEKREGILGTKASNPNVEASCARILAESIRNQRL
>S1.Mha184V [EnzType=Type_I_specificity_subunit] [RecSeq=GNGAYNNNNNNNNTGT]
AERXWKKFPVXPSKRVVATPVRFKLNVANAVIYVKKCVSKHKKXKWKIXHLLLRKRWQNKLKMCKKINKFNRLQPKKYLLKQLLRXKQRTKDVKITANAV
YLAICVWAISVVVKINAIRLFLLCLXLQLLLRLSLRAVKFGFRLPLLSKXRKKHHSYLLIKCLNSKKISLLRQRKNQKRRSFIQLVRXVALXHKQLTVIW
LRRSKNSFPNRKKKKLLLKTXNQXRXKSILLHLVVLLLSILNVIXLKRKMSESVNSNRKQRLHQRLWKRLLTRLKCQNQLTKAIIDLTADXVHSLVLSIP
KPLXPLLLHQVTSLKXSKLANGKTLNTISMAKGLADIPAQXAKCSQRLVQPMVKYNXLVXMPISYNXXAFFYQSMNFLSIFLPKXTHFITKCVIFSLFKE
LGMKKSFLSLLVLGTTMALAACDQAKEAAQSTTDKAVEVKEAVAEKAAEVKSATEAKVEELKAAANDAAQATADKTEEAKKAVAEKATELKTTTEA
>M.PfrJS2V [EnzType=Type_II_methyltransferase] [RecSeq=GCCGGC]
MIQGRTACRSIPARAGTYESKELFMGARTIHPFPARMAPDIAIDHLPPAANDTSVVVLDPMCGSGTVLAAAAERGHTARGFDVDPLAVLMSSVATQTVDT
ELVVSEAERVCTRAQASRVDKPRWSDPETRKFAEYWFAPTQRGQLNRLSRELDRIGDDSIRQALQVALSRIIVTKAPKASLAADTSHSRPHRVATESSYD
VYRGFVSSVMTLKKLLDQRSLVGQVQVSRGDARALDLPDSSVDLIITSPPYLNAIDYLRGHKMSLIWFGHTIPELRSIRSNSIGADRALDSEMLDQVEGM
LRYIKESVVHPDQLPLATISRYAHDLTRFSRELSRVCKPGAKVATVIGNSTLRGNYIENDMLVRLSYENSGFRVVDRSERDLPENRRYLPVKTVDKTSSM
AKRMRTEVVLTVEKK
>M.Tam77409IV [EnzType=Type_II_methyltransferase] [RecSeq=CCRCTC]
MRKLIEVALPLEAINRESSREKSIRHGHPSTLHLWWARRPLASARAVLFASLVDDPGEYLPEEEARKERERLFALLERLVNWDNIKNPTEAEREGGVIWE
AQYEMAKSLARALGEKPPASPKDRERIRALLEQAPPVLDPFAGGGTIPLEAQRLGLKAYAFDLNPVAVLINKALIEIPPRFAGKPPVNSDYRAKAQATDR
FPRAKGLAQDVRYYGAWMREEALKRIGHLYPDLNGERVIAWLWARTVVCPNPACRAEAPLARSFWLSTKRKVYVVPEVQGGQVFFRVEEGKEPPVAGTVD
RRGGRCLVCGAHIPLEHVRQEGKAGRLGARLMAIVTEGEGGRNYHAPTPEHEEVAKEAIPRWKPDTKLPSQALGFRVQNYGLTTHADLFTPRQLVALTTF
ADLVAEAREKAYRDALEAGLTDDGLPLAEGGVGAKAYAEAVGVYLGIGIDRLADYHASLCIWHVNRETIAHVFNRQALPMTWDYAEANPFGEATGSWEGM
VEWVAKALEALPAHPQGQARQVNALEAANGVPTPPLISTDPPYYDNISYAALSDFFYVWLRKALGDTYPTLFRTLLTPKAEELVADPYRQGGKEAARRHF
EEGMRQVFQNLRAKAHPHYPLSLYYAFKQQEAEVNGNGEEEDETPKVASTGWETFLQGLVDAGFQITATWPMRTELANRPRGQNSNALASSILLVCRPRP
QHAPIATRQDFLRALRRELPEALRHLTQGSIPPVDLAQSAIGPGMAVYSRFQAVLEPDGRAIPVREALALINQVLDEFLAEEEAELDAPTRFAIAWYEQY
GYADGPYGDAETLAKAKNVSVTAMAEGGILWAKGGKVRLLRPEEYPPDWNPAGHRSLSAWEVAHGLIKALKEQGETAATSLLARIPEALAEGARALAYRL
YGLAERKGRLAEAQDFNLLAGSYSHLSVEARKTRRAVQEGLF
>Eco2159II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAABCC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>PacI [EnzType=Type_II_restriction_enzyme] [RecSeq=TTAATTAA]
MTQCPRCQRNLAADEFYAGSSKMCKGCMTWQNLSYNANKEGHANTFTKATFLAWYGLSAQRHCGYCGISEAGFTSLHRTNPRGYHIQCLGVDRSDSFEGY
SPQNARLACFICNRIKSNIFSASEMDVLGEAISKAWHGRGIA
>FseI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGCCGGCC]
MTDELFPIPEPLVRPVIALPPHLKELIDLLPLNTPVHRRDLEAKYGRSNYARRIRKIISEYGWEIESRRQSEGANDDWYIRRSDGPVRPQRIRREVPRRS
RETVYRRDDWICQICRMKTDPERGSLVPQCDHKIPADRGGDSDEENLQTLCTRCNLKKRQACGGCALASCADCPFAYPEKFDDVLILHLDREHLKRIMTT
AYARNVTASAVVSDLSDLL
>Eco4276II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAABCC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>AhyRBAHI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GCYYGAC]
MRQALDKTLRNQLESTVLAAREVVETACREEIERLGVADATVPAFLNDAQRKLRNRLRAHARQLGDPLHDNGKQETAHLLAEMAYEQWHRMLFARFLEQN
DLLMYDAYTPVTLDECAELLEEETDCASAWELAGRLAAKMLPQVFRVESPVLAVRLPINHQRTLEGLIMALSPAVFEAQDSLGWCYQFWQSKRKDEVNKS
GVPIGADELSPVTQLFTEPYMVSFLLDNALGAWWAKCRLTDDHLRHASDEQELRERAAIPDVPLSYLRFVKVTDTQGDRWQPAGGWLDAWPDDISELKTL
DPCCGSGHFLVALFLMLVPMRMHLEGCDANTAIERVITQNLHGLELDSRCIEIAAFALALEAWRFPNAGGYRPLPALQLACSGQDVLAASDEWQQLAKQN
PELEPALDWLMRQFSDAPVLGSLIDIKRAHYEGKGEGAPAPWQQLLDAQAQSDDESQDASVAAQGLALAAKLLAGSYHLVATNVPYLGRGKQCSVLQDFC
DEYYPEAKADLATVFLERCLDFNPLGGRSTIVLPQNWLFLTSYKKFREKLLTEQTWNILARLGEKGFESAAAAGAFVALITLSKGVAPKEALLSGVDVSE
FKTVLDKSDKLISAKMINVEQLKQLENPDARVSFEFFEGALLEKYSAAYIGQRSGDGPRYIQSFWEKPTLSNGWVFFGSTVTDVFEYSGCSQVFLWENGQ
GTLKKYQSELAETKYASGGWKQGWQAWGVKGVRISQMRRLPVTLHTGPHFDNNTAVLVPDDYADLLPIWCFCASESYNEAVRKIDQKVNVTNATLAKVPF
DLEYWSEVAQKQYPNGLPEPYSNDPTQWIFHGHPCASVVWDEESKWTKEGLVRFEETVLQVAVARLLGYQWPAELDDEMELADEQRQLVDEVAKLNAFAD
EDGIVCLPAVRGEAPAHTRLEKLLVAAYEDDWKAGALDKLLTAVGSKSLELWLRDKFFEQHCKLFQHRPFIWHIWDGLKDGFSALVNYHKLDRSGLERLI
YTYLGDWIRIQEQGVKEARDGAEERLTAAKNLQQRLEAILAGEKGLDIFVRWKTLAEQPIGWEPDLNDGVRLNIRPFLKAADVGKKGAGILRFKPNINWN
KDRGNDVASAPWYTLGLQYGEKEGARINDHHLSLEDKRAARANQPK
>HpyGII [EnzType=Type_II_restriction_enzyme] [RecSeq=TGCA]
MFQEITNKRKFFASSSTGEQFENKFRNELKKHFSEINGDLTEELSHIEEKPNKEIKTTFNQLKKQVLEKNHPHTLKNPFSNLTSHFLYQPFGSQNYPDFL
VFIFDHVVGIEIKFSKNDKGEKNLQTSRPMWNSNLPKPNAIYVYGVANADITFFKGSDILSYETREVLLKYFDTLDKDEESLKNALKDLENPFGFAPYIR
KAYEHKKEFSNHHQIESFFSPNHILRERNVLEFLKTLTH
>Eli8509II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CCGGAG]
MNPVEIADAVTELAQLPFDGAEFPFQFLTAFGNKKTTIDRLRAGNTNQSDIAGGVLQRSNIHIATAAPGAVADMLTKLRNSPKTSSQKARFILATDGNEL
QAEDLTTGETVVCQYADFANHFGFLLPLAGITTVAQIRESSFDIRATGRLNKLYVTLLQDNPEWASADRRADMNHFMARLIFCFFAEDTDIFHAGYKFTE
TVEWMSGGGADNTHEVIAELFRAMNTPLKDREAAGTKTWANGFPYVNGQLFSGSIDVPRFSKIARSYLIHIGNLDWTKINPDIFGSMIQAVADDEERGAL
GMHYTSVPNILKVLNPLFLDDLRGRLDEAGENARKLLNLRNRMAKIRVFDPACGSGNFLVIAYKQMRAIEAEINKRRGEPERRSDIPLTNFRGIELRDFP
AEIARLALIIAEYQCDVLYRGQKEALAEFLPLDSQNWITCGNALRLNWLSICPPTGTGVKVRGDDLFQTPLDQSEIDFENAGGETYICGNPPYLGSTWQD
AEQKADLQRLFGHRTKSWKSLDYVAGWFIKAADYGLHTPTVSAFVSTNSICQGQQVPILWPLMFATGHEIVFAHTSFKWANLAAKKAGVTVAIIGIARPT
SSLKKLYSPGPNDGSILKEVEHINAYLVPAPDVTVNKRSAPADDRAQMDAGGKPVDGGHLFLTPDQRRAYLREWTQRTKVVLRAFGASEYIRGDVRHCFW
LSDEDVVGLDPRDQLSERLEAVRLARSKSPKLPTRSAAAWPHRFEEVKQTGREVVTIAPKVSSENRDYLPAGLLPPGSILTDLAFGLYDAPLWNLGVLCS
RLHLVWIATVCGKMKTDFRYSNTMGWNTYPLPKLTEQNKADLTRCAENILLAREAHFPATIADLYNPEAMPENLRRAHEENDETLERIYIGRRFKNDTER
LEKLFELYTKMTGKGKAA
>AteTI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GGGRAG]
MKLSEQKIRAKEFSQRWANTGDEKSESQKFWLDLLENVLGVKDPYSYIDFETRVKLDNTSFIDGYIEKTHVLIEQKSSKKALDKAIEQSDGSYLTPFQQA
KRYSTELPYSQRPRWIITSNFKEFYIYDMEKPTGEPEKIRLKDLENEIYRLNFLVREEEDFIKKETQISIEAGEIVASIYEALLMEYKNPESEKTLHSLN
ILCVRLVFCLYAEDAGLFGNKNMFHDYLNKYKDTNPRTALINIFRILNQKEEDRDPYEDKDLLAFPYVNGGLFSDMDIEIPRLNPNIVDIILNRASLGFD
WSEISPTIFGSVFESTLNPETRRTGGMHYTSIKNIHKLIDPLFLDDLKEEFEEIKEIAQVKQKKQRLEELHNKIANLKFLDPAAGSGNFLTEIYISLRKL
ENKIIEEKIRKKDVISISFIDESSEELENPIRVNLGQFYGIEINDFAVTVARTALWISEDQMMKETMNIIHQKMDILPLKSYANIVEANALTMDWNDLVK
AEELDYIMGNPPFVGARLMDKEQRKDLEIVFGSKYKNIGMLDYVTGWYKKAFDYIKATKIKVAFVSTSSISEGEMVGYLWKPLFENGLNIVFAHESFKWI
SEASDMAQVYVVIIGFKDGIFDEKKYLYSNNSVKSVANINAYLKDAPNVFVERIRKPIFDVPKMHKGCQPTDGGNLIIEADELEAFLKKEPKAKKFIKKL
IGAREFINNKDRYCLWLVDATPSELRAMPLVMERIRKTKEMRLAANSKGTQKLAETPHLFRETYNYKAFIAIPRVSSSNRKYIPIGLLRDDVIATDALLI
ANSINLYEFGVISSNVHMGWMRVISGRLKNDYRYSAGIVYNNFPWPSPNEKQREKIEKTAQKILDARALYPSSSLADLYDDLTMPAELRKAHMLNDKAVM
EAYGFDWRTMTESECVAELMKMYKALLGDDDGSF
>MseI [EnzType=Type_II_restriction_enzyme] [RecSeq=TTAA]
MTHEPTDDPDFIVMAASAANLADRYVASEDDPWVGSPFEWILRVPSRTKGAVGELLVSEWANAKGLRVKRSGSSDADRVINGHRIEIKMSTLWKSGGFKF
QQIRDQEYDFCLCLGISPFEVHAWLLPKDLLLEYVIGHMGQHTGASGSDTAWLGFPADEPYDWMRPFGGRLGHVEDLLLAAGPGPY
>Eco8368II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAABCC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>M.Hpy32VIII [EnzType=Type_II_methyltransferase] [RecSeq=TCNNGA]
MQTLFKEVTPKRYVNGNEMKENSSNVLDQYFTKPSVALKCFQKACEIIKKYENLDDFIFLEPSAGDGVFYDLFPKNRRIGIDIEPKRDGFIQCDFLNYKL
PTHQKVICLGNPPFGHRGVMALEFINHARSCDFVCFILPMFFESQGKGSIKYRVKGLNLLYSERLEKNAFIDFKNKEVDVHCVFQIWSKKYQNKKSEFSW
YKNRHKEPFGEYIKVFTVSLAKNRECGKEWIFNQKASFYLSSTFYKSTQIVENFEEVKYKSGIAVVFTSANKALNTKLKKLFKEIDWTKYASLATNSCYH
LGKSHIFQALYDHLDSLKDN
>M.HpyG272VI [EnzType=Type_II_methyltransferase] [RecSeq=TCNNGA]
MQTLFKEITPKRYVNGNEMKKNSSNVLDQYFTKPSVALKCFQKACEVIKKYENLDDFIFLEPSAGDGVFYDLLPKNRRIGIDIEPKRDEFIQCDFLNYKL
PTHQKVVCLGNPPFGHRGVMALEFINHARSCDFVCFILPMFFESQGKGSIKYRVKGLNLLYSERLEKNAFIDFKNKEVDVHCVFQIWSKKHQNKKSEFSW
YKNRHEEPFGEYIKVFTVSLAKNRECGKEWIFNQKASFYISSTFYKSTQIVENFEEVKYKSGIAVVFTSTNKALNTKLKKLFKEIDWTKYASLATNSCYH
LGKSHIFQALYDHLDSLKDN
>M.Mtu22103III [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRD
FASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFL
LSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGEL
ADVDVGIVTGRNSFFTFTDAKAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWW
STPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDV
DLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>SstE37I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CGAAGAC]
MQDQEVEEFIDRWKNTGGSEHANYQLFVIELTGLLGLDRPNPATDDDSNDHYRFERPVIFAHTQKRSTGFIDVYRAGHFVLETKQGVNQKKSRAADLTTG
TAARTQKRTGHGVRGTAAWDDTMLKARNQADNYARAVARDDGWPPFLMIVDVGHVIELYADFSKQGQGYNQYPDGNRYRIFLDDLRKDETRDLLRTIWTE
PFTLDPSLKAAEVTRDIAAHLAELGKSFEGQGHSSETVARFLMRCLFSMFAEDVDLIPRGSFTELLRKLRGHPEHAEPALKGLWETMNTGGFSQVLMQDL
KRFNGGLFRDADALPLNNLQLGLLIEAAEADWKQVEPAIFGTLLERALDKRQRHKLGAHYTPRAYVQRLVTPTIIEPLRDDWRDVQTAVQRLTEDGKKDE
ARKLVADFHAQLCETTVLDPACGSGNFLYVALEMMKRLEGEVTSLMVDLGDTKPLITVDPHQFLGIELNPWAANVAELVLWIGYLQWHYRTHGQAAPSEP
VLRDFRNIANADAVLKWDDRTPRMDAEGNPVTRWDGVTTMRHPVTGEEVPDPAARVQVYDYAKPRATVWPRANFIVGNPPFIGARKIKSALSEEYVETLR
AVYQNVPNTVDFVMYWWAKAAEAVKHKSARRFGLITTNSITQDYSRKLVDKNISGGKPKLNLVFATTNHPWVDSSDGASVRIAMTVASGRKIKKPRILEN
AGTEDQKEKSVDRINSRLADVPDFSELQPLRSNYRMCFQGVVPAGDGFKLGSSEKDALEMRLSEKEKAKIKRYYIGKDIIDRPRHQSIIDLFGVDLEELK
RDYPTLFAYLLDRVKPVREENSRPQYKKLWWIFAEPRPALRSAIGGIRQFIGTTYTAKHRVFQFCGSEIVPDAMVYAIATDQPELLACLSSRVHLVWCKG
STGTLEDRPRYNSANTFSPFPFPDLGNNERESLRTLGEHLDAHRKRQQAAHPKLTLTQMYNVLEKLRAGEPIEGKDREIYDQGLIGILRDLHDQIDAEVA
RAYGWPADLSDEDILFRLVALNKERAEEEAQGHIRWLRPDYQNPTGQQATKGKQAELDVGMAAKIEKAPWPKTLPDQIAAVREALAEMGEATPEQIARRF
MRARTTSVAPLLDSLAALGQAEKGGDGRYAA
>HgiCI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGYRCC]
MNYQRSFEDLEFNAIKWWPQELSATVAEASVLPILISSQDLFISILKLSGTHPEQIFDVINAAQISANLFLKHLVVLADYGGEMIKRLGKEFQEIFPRMD
STLEYYMNYTFKGEQYTYIFKKLPIKGLDNSKLAIDGKAIIEIKPLSDLYRDMIMILLYGSTTEQFNLAGLEKCEIGTILGKNEIIYTYITQKYLYVSRI
TNGANTNSLGQIAQTYVCDILSKYLPNDYSVTRNGKILLSDLNSQDSTKTSFDILVEFADKKVGIEVSFQVTTNSTIERKAGQARDRQNRMHAHYYWIAY
VIDGAGNFERSGAVRAICRYSDCTVAYSESEIAVLAAFIQEKFNA
>M2.Dsh12II [EnzType=Type_II_methyltransferase] [RecSeq=GACGGC]
MDPFMGSGTTAIEAVRYANSVHGVEVDPYARLIASVATQKYSEDEIGKISFLVSEIGRAFEHQSLYDDLKPRLANIEYWFGDENFSDLLKLKSAIFELTD
EGKVREFLLAAFGDIIRACSKAERQSLKPYISKKYEKKPKRVLDEFLRIASKYIDAVSESQKHKSDGIVWEGFDATNFDVSTSVDLAITSPPYINAMDYT
RCIKLESAWIGTADDDAIKTVRNAQLGERVRRNREVREDFVYSISRQHLGELETIDEARFKTAVVFFDDMRRNMEAVFRALRPGGFYYIIIGNSRIRGVD
VPTHQVIAEIAGQLGYKWDKYFLYRIRDHRTSIPRGDRGGKIEFEHALGLMKP
>SelPI [EnzType=Type_II_restriction_enzyme] [RecSeq=CCWGG]
MSRTRYDYAQKIATFLRTHDEPVHAKDIYELFNVSQGTVRKHLKNYLDANPNAKAKVTGVYPVNYSEEISSFLAENIGAIDVEDIYNLFKVTPGTVDNYL
REHLHQYPNDIPRIIGFYPKAETVVALAETEIGLVTGENLFEINAHCKRTIDAITKPKHYKFIEGLLQSYLEEDGQTIRVSKNQLINSLYENYVDFVHES
DNGIISRAGGINEKILIRGLENAGMVLGQNFKKTGNNSEGDLQVECRAQNSTKILYCEVKSYAARERLLRGIQDIPHPDKVAVGFFLDPDEFNPDRTQTL
LAAGPLAIYMPDVTYEALSANSIIQTTRRQDMLYRPLSRFIDDMCNFSRSGNLPRYLQRHEN
>Nbr128II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=ACCGAC]
MNAVEIEAAISELACQPFDAAEFPFAFLAAFGNKDTALKRLRAGNNNASDVPGGVLQRNNIHIAVCEAGTVGETLKALRASRATTKAKAKFILATDGQTL
EAEELGCGETITCDYPDFPNHFGFFLPLAGISTIKEIKDNPIDVRATGRLNKLYVELLRENSDWAKDERRPDMNHFMARLVFCFFAEDTDIFNGEGLFTR
TVDQYSERDGANTHQVLSEIFRAMNTRIADRAEAKFRPWADQFPYVNGGLFSSSTEVPRFTRMARTYLLHTGSLKWREINPDIFGSMIQAVADDEERGAL
GMHYTSVPNILKVLNPLFLDDLRAQLAEAGDNERKLLNLRKRMARIRVFDPACGSGNFLVIAYKQMREIEAEINRRRGEMHLGSEIPIKSFRGIELRDFP
AEIARLALIIAEFQCDVLYRGQKDALEEFLPLDAQNWIICGNSLRLDWLSICPPTSTGVKLVADDLFGTPLNQTEIDFGNEGGETYICGNPPYLGFTWQS
SEQKQEMKAIFEHRVKGWKSLDYVAGWFMKAADYGSRTRAISAFVATNSICQGEQVALLWPLIFASGHEIAFAHTSFKWANLASHNAGVTVVVVGISRDT
NQKRKLLDDDGDGVIAVREVDSINAYLVPGQNVVIEPIPRLVRDSDKATMVWGNKPTDGGNLSLAAEERDKFLAENPETEKFMRPYFGAAEYIRGLARFC
IWVNDAEANVAAQIPALLRRFERVRTFREESKAAETRPAADYPHRFRQIQGVAISHALIVPQVSSERRGYLPVGLLPPRSIVSNLAFALYDAPLWNMAPI
ASRLHLVWIATVCGKLETRYRYSNTLGWNTFPVPLLTEQSKADLTACAENILLAREAHFPATIADLYDPDNMPENLRRAHERNDEVLERIYIGRRFKNDT
ERLEKLFELYTKMTAAAAKAKPTTKATRRKKA
>SnaBI [EnzType=Type_II_restriction_enzyme] [RecSeq=TACGTA]
MLNYKINPGLHINCGFIVTGEAVRNAIMQTNAILGDLPANLYKSIDYKTTSAMIGSVFCDRLADVVGAIVNPIEKGHPDLVPPAAINCTEEELRNYPQGL
EIKCTIGSIETGADLRAGQTRIESLTGITWQAHHREVAELLGLVWDFFEDGRAFNYPAISGAFYSDDLCEDDWGQISGTNGRNTKVSAMVASGKAKMGRG
WVAVVEKDEYLTAYSRILRFRFP
>Nal45188II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=ACCAGC]
MSDKSRSARRGQELERPTRVLRRGRACRGDTEPEHRGDSVSQQFLGESTAHKGTWHQPDASELGVTPRYYWRGNGTHALEVAHGTATTRPSNTNVRKLWK
ARHGNSAAPLLLVVAWPEDDLRRAAVCGPRGDDPVVVDLDLPQAERLAQAALEEPNRHNAARFVAGALQSGSDEAVGLLNRGLFATHELAEGVPARPDWT
EAVERSKPLLAKRDHELIRDLGYTVDPSGQHHVLRAEGHSRAVAVFLQPSEQADQPSARHGGQTPVSYALAAADRDNLKWVVAVRGGNLRLYSTETAGAA
GQRGRAETYTELNLPLLQTDRAGYLSLLFSPEALAPDGTASQVRTASQDYAAGLSQRLRDRVYTRAVPHLAVAVARERGGVTESDLALHYRTALTILFRV
LFVAYAEDSRLLPLHSNGDYTDHALKTLARRLADHANAGEDLGFDNPLTSVVEPATDADSTELWDRAQQLFTAVDKGHVRWGVPQYNGGLFSNDPSANPV
GGIIADLRLSNADFGPALMALMVDKTEDEVAGPIDFRSLSVREFGTIYEGLLESELSVAADDLTVDTEGTYRPAREGEDVLVEAGEIYLHNRSGARKASG
SFFTKPFAVEHIIGQTVEPALQEHLARVAAKLAGGKEADAAAMLFDFRCADIAMGSAHFLTAVVDRLEATYTEFLTRHPIPRVMEELDTLRAAALEHLGN
LADTVEIENSSLLRRMIARRCIYGVDLNPISVELARVSMWIHTFVPGLPLSFLDHNLVHGDSLTGIGTIHEATEELTADGVQPSLFDNPILEALDAARQP
LQRLANLADATATDVQRAREASEAIADAIDPVSELFDLVVAARLGETEPPALTSLEKAPDLLSEVARQAVRLLRPIHFPVAFPEVFLRENPGFDVLVGNP
PWEKPQVEEHEFWGRHFPGHRGLPQREKETATANYKVQRPDLLKALHLEQERTRRLRDVVTSGPYELGVGHADFAQLFAWRFWALAREGGHVGLVMPRSA
VLSAPGMAVWRNEIFEKGAFNDVCLLLNNRYWVFDEVEPRYTIALLALQRLSSPAAQIRLRGPFRSFEDYRANTDRATSNVQVSELKSWGASAAFPLLPT
PKSLQIYRSMAQHPRLQDITRFVMRPVQGDVNATEAKRRGLLEFSEAPPPDSWPVLGGSGFRLLEAETGQVYAWTDRSTMREHLFERRNRQARLQRSAFS
ALDSETLLDAETLPCMRPRIAFRDVARATDTRTIIVALVPPEVVLQHKAPFLLRTAGTQKDEAYLLGVLASIPLDWLARRLVETTVSFSVLGDLAIPLPD
VAHPGYVQTVSVAGRLAAVDDRFKAWADAVEVPAASVSGSERDGLVAELDACVARLYGLTEDELVHVFETFHVGWDYKPRLEAVLTHFRTITWDPAPTEA
A
>M.NspIII [EnzType=Type_II_methyltransferase] [RecSeq=CYCGRG]
MLQEFVAVVSLSSISNITSEVESNALREIDHLDKQLQSHFQTKFVIQPSLTRLLVSFQANKTRPIYRWYKYKEAFSASLVELLCQKYGITQGKVLDPFAG
SGTALFATIDIGIDADGIELLPIGQQIIDTKKILDVEFTPDDFTRLKTWLDLQVWKQFETEVTLPELRITKGAYSQATKTAIEQYLEACNHENHRVKSVL
HFALLCVLESVSYTRKDGQYLRWDYRSGRGQGKKPFNKGTILEFDHAITNKISEIINDLEPSTQQLELFSFKKSQVQINLRKGSCLNIMPCLPNAVYDAI
ITSPPYCNRYDYTRTYALELALLGISEQELINLRQEMLSCTVENRPKDLLAINQDWELALRVADSQTLLQAILKYLDNQNVQGLLNNKGIPRMVRGYFYE
MACVIQECFRVLKPGAFLLMVNDNVRYAGASISVDMILSDFAEKLGFVVESILVLPSDKGNSSQQMGNHGREPLRKCVYVWKKQ
>MjaV [EnzType=Type_II_restriction_enzyme] [RecSeq=GTAC]
MDDKSYYEEIESILRQILQPIEKISFSTFIRVVSGYKIIPIDLSKKEDKELINDLAKACNEVIEEIKKTGGVKTKEGKTPKRVNEVGNHIEHYVKDVLNK
YGYAITPKTKKGKQKSTGYPDIEFWYKGKKERDGRVVYIEIKTFNEKNINSSHRTFYASPSKDEEGVKIRYDAPHLCLSFKIEKLGRDYYATGFKIIDLS
KLKGGIKREFNASNRELYKKDLIIYEKDLK
>M.HpyFVI [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MVSNTTLQKNLDAFYTHPKIARFCLDLLKDLINQNLGLDLNAFHFLEPSAGSGSFVGALKGLGIADCLALDIAPKAQGIQKKDYLLELIGFNKKRIIIGN
PPFGHRGKLALDFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIAPPKIRHNDFI
TYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALMIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>BspQI [EnzType=Type_II_restriction_enzyme] [RecSeq=GCTCTTC]
MRRLAKNSRNDSYLSNRDYQEIVRENTTTISFPLKEKHTLTLTKKIGLNQTAGFGGWFFPDSPCLLTVTVLSSFGTKVTSKTFSLSKDWNRVGLAWINEH
SSDTMSIVLEFSDVEIVHTWGLTCDVFNVHELIIDAIEDQNKLIDVLNQEHLSPETYYLNHDSDTDLIENLESTEEIKIVNQSQKQISLKKCCYCQRYMP
VNILVRSNSSFHKHKSKKTGFQNECRACKKWRINNSFNPVRTKDQLHESAVITREKKILLKEPEILQKIKNRNNGEGLKSIIWKKFDKKCFNCEKELTIE
EVRLDHTRPLAYLWPIDEHATCLCEKCNNTKHDMFPIDFYQGDEDKLRRLARITGLDYESLVKRDVNEVELARIINNIEDFATNVEARTFRSIRNKVKEV
RPDTDLFEILKSKNINLYNELQYELLTRKD
>M.MkaDI [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MTTFAGKAAASAEKVRGGYYTPPAVARFLARWVCEAGPRILEPSCGDGRILRELAALTSQAHGVELVAAEAGKSREFACVDVDNFFTWLHTARPGSWDGI
AGNPPYIRFGNWASDQREPALELMRSAGLRPTKLTNAWVPFVVASTTLVREGGRVGLVVPAELLQVGYAAQLRDFLVSHYREITVVTFERLVFDGVLQEV
VLFCGVVGAGPARVRTVNLADAGGLGAASLTGETAPALLHEKEKWTTYFLDPPQIELLRALRRSGTMVRLRDLAQVDVGIVTGRNSFFTFTDAEAGALGL
RQHCVALVSRSAQLSGLVYDEDCRASDVAAGHRTWLLNAPTTPADAAVAAHIAAGEAAGVQLGYKCSIRTPWWRTPSLWIPDLFMLRQIHHAPRLTVNAA
AATSTDTVHRVRVDPTVDPARLAAAFHNSVTFAFAEIMGRSYGGGVLELEPGEAELLPVPPPAYTSTELARDVDLLLKANEMEKALDIVDRHVLIERLGL
SADLVASCRAAWSTLSGRRRNRGSR
>KpnI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGTACC]
MDVFDKVYSDDNNSYDQKTVSQRIEALFLNNLGKVVTRQQIIRAATDPKTGKQPENWHQRLSELRTDKGYTILSWRDMKVLAPQEYIMPHATRRPKAAKR
VLPTKETWEQVLDRANYSCEWQEDGQHCGLVEGDIDPIGGGTVKLTPDHMTPHSIDPATDVNDPKMWQALCGRHQVMKKNYWDSNNGKINVIGILQSVNE
KQKNDALEFLLNYYGLKR
>Sno506I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GGCCGAG]
MTDVERFISRWTGRDGGQERANYALFLTEFCRLIGVESPEPAAHATELNAYVFERAVTFREPDGSTAKGRIDLYKRGCFVLEAKQSRQGEGPKAAAGGEQ
QQALFPIEEPPQGRRVSRRWDVLMMNARRQAEDYAKALPPAEGWPPFLIVCDVGNCFELYADFSGQGKNYAQFPDRQGFRIFLQDLRSEEVRERLKKVWT
DPHSLDPTKRAAKVTRDIAERLAKVSKFLEEQRGADGRPRYKREAIASFLMRCLFTMFAEDVELIPKESFKRVLDRCRANPELLPELVGQLWEAMDAGTF
AFAIEAKVKRFNGYLFKDRTVLPLPREEIGELFEAAKANWREVEPAIFGTLLEQALDPVERRKLGAHYTPRAYVERLVVATVIEPLRAEWDLVRATAERL
SSEGNPGAALAEVTKFHAQLCGVRVLDPACGTGNFLYVALELMKRLEGEVLEAVADLGGQEALGLDRHTIDPHQFLGMELNPHAAAIAELVVWIGFLQWF
FRTQGGQPTEPILRDFKTIKAMDAVLLHDGSEPALDEASRPVMQMDAEGRRVPVLAFRNPRLPPWPEAEYIVGNPPFIGGKDVRGRLGDGYAKALWAAHP
HMNESADFVMYWWDRAADLLTRRGTRLRRFGFVTTNSITQEFSRRVMAARMKAKKPISLLMAIPDHPWTKATRDAAAVRIAMTVASTGKHDGVLSEVVRE
GGLEGDAPTIEYADYRGEINPDLTVGADVTSATGLRSNDFISSPGMKLHGSGFIISPAEAEAVGLGKREGLEAFVRPYRNGRDLAARGRGVLVIDLFGLE
EEEVRRDYPEVYQRLLTVRAARDEQCLRSNTPDAIEYAKRWWVFGKPRQELRGALRGLRRYVATVETSKHRVFQFLPEDIVPDNMLVCIASDDAFVLGVL
SSRTHVVWALRAGGWLGIGNDPRYSKSRVFDPFPFPDASEPLKVDIRAVAEELDAFRKARQVEHPRLTLTQMYNVLEKLRVGASLDEDDERIRDEGLVLV
LKELHDRLDARVLDAYGWPHNLSDEDIIARLVALNKQRAKEEARGLIRWLRPDYQKARAGLATSVRPEETQDSMALVVDAAREQKPQFPADEVERTAAIM
AALANASGTVDAAAIAAGFRQGKRVEPQIQATLASLVRMGFASSPDGRSFMFRRVA
>Adh6U21I_(Adh6U21IB) [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAANCAG]
MATSDNGRFLRFWHEVSAEKLCFDCTSTVESESRAEKWYAYNKGGEFRRWFGNNEYVVNWQHNGRELKEFVEEINKLRPGGRLKNQEYYFRESITYSSLS
SGLFSARYNPVGFLFDTKGSCIFPVSVNKNDLLGLLNSNVAQAFLNILCPTLDYSSIGINSIPVKLPESNEITTSLIDAAKHDWDSYELSWHFNRLPILH
QELGLLLLEQSYQKLRSFMQQRTSEMQRLEEENNRLFIEAYGLQDELRPEVPLSEITLTCNPHYRYGGNLTDEEREARLQSDTLVELISYAIGCMMGRYS
LDREGLVYAHAGNEGFKALVEEGAYATFAADEDGILPLSTDSWFEDDVATRLEAFVCNVWGKDTLEENLQFIADSLCLAAIKPVKKGGETSRETLRRYLS
TQFYKDHLKTYKKRPIYWLFSSGKEKAFECLVYLHRYNEATLPRMRTEYVTPLLGQMASRIERLRLQQTEASTSDAKRIGKEIDSLTKQLSELRAFDDQL
KHHADMRITLDLDDGVKVNYGKFGTLLSDVKAITGDKGE
>Vtu19109I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CACRAYC]
MNTNNVKKYAPKARKAFIEAMSKQAAKYGITKNKIEPAEQKGDLVLIGDRPFPASVIKPRERLVKRIEQQGFDQVMEHVAYSWFNRLCAIRYMERKGFLD
HGRRVLSAADGSAGVPQILEECLDVELPGLDQAKVRELKLDGTKDEELYRELLLAQCHALHQAMPFLFEAVADESELLLPENLTKTDSLIRDLVASVPDE
DWDHIEIIGWLYQFYISEKKDQVIGKVVKSEDIPAATQLFTPNWIVQYLVQNSVGRHWLQTYPDSPLKEKMPYYIEPPEQSPEVMSKIADITPSEVSPES
LKIADIACGSGHILVETYRVMKAIYEERGYRSRDIPQLILENNLFGLDIDDRAGQMAGFALLMLAREDDRRIFSRNIRLNILSLQESANVNLPSLWRSLN
LTGEWQSGASQGLFEEEQQDLSSFAADNRYQLIQRTLERFKKAKTFGSLIDVSHDDYEELKELFQTLCELEIAGDSMQKAAASQLIPFVHQSIVLSQKYE
SVLANPPYMGSAYYNSTLKDFASLKFDEWGGDLFTCFIKQSLDMIAREGSVALITIGTWTFLPTFEAMRRQLLDCCIFTSIIDNGRGVWGSDHGSLMFSA
KKIDGDLCGNKVATKYLSRGKGKVQQVNVIRSKYLSDKPLYIEQDLFKKIPGYVISVGATPELLNQFLESHRLRDLADARQGIKTGDIAKYLRYWHEVSY
SNIKFDCNSSDESMVASEQWYPCNKGGEFRKWYGNQDYVLNWKSDGFEIRNFKDESGKLRSRPQNTHLFFKEGITWSTVTMGRPSFRYSKPGRIFESGGS
TIFTNEDIYYLLGVLNSKLGEVSVGILSPKVSLGEGAVSKFPIPDLSSDKKDKISECAKTLVQIFSTIDSWDESTFDFTGLFDFPSQDNLEVYATRWIEK
YKSILNDIKRQEDLINSIVLGSGFEKNTDEVDPQDYGLPEINSSVIVERIVSFAIGCMMGRFSLESKGVVYADSNEEKGNSSSFELIKTTSNFSVDEDGI
IPITGIEWFPDDAANRLHEFLMSVWPKDRLGENVDFVANSLCNSSMKAKQNESTSDTIRRYLSTQFYKDHLKTYKKRPIYWLFSSGKQKAFECLVYLHRY
NEGTLARMRTEYVIPLTAKMAAYAQKLENDKEASGSAAEVKRLEKEIAQLHKQQAELSEFDEKLRHYADQRISLDLDDGVKVNYGKFGDLLAEVKAITGE
APEALS
>EcoTWII [EnzType=Type_IIG_restriction_enzyme/methyltransferase] 
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>S.ZmoI [EnzType=Type_I_specificity_subunit] 
MKGLDANVGTAREIETVQRFDAEYFRRAYVTCEEAVTQSGRSIRLRDEGASVQRGQQPTYSEDGIPVVNSKHVKGMFVSVTDENRRAVLMGRGPLIKEGD
ILINGTGLGTVGRVSLYVSEENAIPDNHVTIARSKTFEPEFLAAFLNGKYGQAQLQRRVRGSSGQVELYPLDILEVSVWRASAALTARVCKLYATSFEKR
DAASRRMAEPGDLLLAELGLLGWTAPQPLSFTASANSAFTAERIDAQFFRPLFAEVNAKLLATGSACELGSILSTNARGRQPQYSDDGLPVINSKHVRTN
RVILVDNRSATDAGSPIVIETGDVLMNGTGEGTIGRAAPYLHNRRALPDNHVTVLRTDRVDPVYLAVFLNSPLGQWQIERHIKGSSGQVELYPSDIARIM
VWEAPEAVQQSVRAAILSAFDEERRANDLLEAAKIAVEIAIESGEPAAMAYLDHAAGTI
>M.PboPX4II [EnzType=Type_II_methyltransferase] [RecSeq=TTCGAA]
MRRDLGDFQTPPELAAALVRRMGPIGSRWPRVLEPTCGRGAFLEAVLSAPDPPREVVGVEIQASHGAEARARLADRPAIHRASILHADLFELDLRVDLPW
IEHGPLLVVGNPPWITSAELGRLGSDNLPPKRNVKGLSGLDARTGAANFDLGEAVWLKLIDELADQTPTIALLCKTSVARAVLQHLHRTGRRVADAELIE
IDARRWFSASVGACFLKLSLGVGETTGRVAVFDAIEATRPRRTMGSLQSVFASDLDALEALDFAFGRSLLCWRQGVKHDAAPVMELIGTADGTFRNGLGE
EPLVEPSFLYPLVKGADLIHPARERPRRALIVTQEKLGAETRDLADRAPRLWAYLQARAAWFDKRRSSIYKAAPPFALFGIGPYTFAPYKAAVAGLHRPS
RFRAVGPVDGRPTVFDDTCYILPCQSALEAAVLTAACNDPITREVLRALTFADAKRPVTKAVLQTIDLGGVLERTDASALVDRVDEVLRDDLGLEGETGD
AIKAEVERLISLLSLADGGKLGRTASPDRPA
>M.Mtu37004II [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGSWDGV
AGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDDGRVGLVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEV
VLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGELADVDVGIVTGRNSFFTFTDAKAQA
LGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAPRLTV
NAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDG
LGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>AvrII [EnzType=Type_II_restriction_enzyme] [RecSeq=CCTAGG]
MEEDLDLSENIEAASAELTTLYQVAADAMKDYIEIYLALSKQSDGFSNINNLDLTSRNRRLVVIHGLSLELDPDTSTPEEIKREAERMLAIALDTESAIT
AGVYEKMRLFASSLVDQLFEQTDELNSLSSEYLSANPGFLPFFQQLAGLRSKSELKREVGNASDNSISKAVAERILERIIRNLRIRTFSKEKLLQAVEPT
LEGIVRDLVGKVLLENIVADALSDLQVPFMRESEYQSLKGVIYDFRADFVIPDAQNPIAFIEVRKSSTRHASLYAKDKMFSAINWKGKNKRLLGILVVEG
PWTRETLRVMANVFDYVTPLTRVSQVAEAIRAYLDGDKTRLKWLVNFSIEEADHDNIT
>CpaAI [EnzType=Type_II_restriction_enzyme] [RecSeq=CGCG]
MIFYDFNDTVNCFMFAYMYELELSLNDSKTSLLDVSFQSDEANTDRPLKVFIKEFIENSFNKLPQSKLETQLNLNLSSYKENFHYKYFSNISITKVFLPD
ELSIEHKEQIAENKSSVYTNPDLFFKISDGTNTTYRSIELKSTKNNAIPGSSIQQIDADEWVIFVKHNKNSIELTTGQYIHSINSKLQFPDRSPRPQVAF
NELTSWNNTNRISNDNLLVYSINSDDEKLKYDLISDWQYYLADRWIEVLFSSSIKNNEPWYNNNLRKFIIKFLEKYELLSEDEKGNLKATINSLIK
>Asp103I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CGRAGGC]
MAGSDAIIVGEGWLSEHYFSTDATKESFRAKVSERRKFWDDEDKEGRETPRSRFISARQDLERDLALMAELLDPSAEAAAVRDGRTADDVAADVHERLIG
IFGLTGHGLVLDRTGPLLRVSAPGVEKHAPLVVLSARPIGALEDLLARDAVTLPEPVQLTEDDEEIKSVARLTSSLFVAEEAPEFVLVLAGRWALLAERD
RWAEGRYLAVDLQLVCERNDTKKAGEIDRALTCLSAESIAPDAEGAIWWHGVLEASVKHTVGVSKDLRDGVRLSIEIIANEVLERRRAQGLDPLPATEAQ
ELAKQSLRFLYRILFLLYAEASPELGVLPVGAPEYDQGYSLDRLRELIQVELATPQAHSGTHLYESLAVLFRLVDNGHAPLTGGDDSEAAEGLTFNALRA
DLFQPAKTAHIDEVRLGNAALQQVLTHLLLNKESRGKDRGFISYAELGINQLGAVYEGLMSYTGFFAETDLYEVAKGGDSVKGSWVVPVDRADDISPDDF
VKSIDPRTGEKRPVLHPKGSFVFRLAGRERQQSASYYTPEVLTRFTVGQALEELLDQDGTTSAADILTFTVCEPALGSGAFAIEAVRQLADQYLKRRQAE
LGVRIDPDEYPRRLQEVKAYLALHNVYGVDLNSTAVELAEISLWLDTMVSGLSAPWFGLHLRRGNSLVGARRAVYSRNQVSTKSWLTEVPRDIPLTSLVD
DVANDRVAADGIHHFLLPADGWGAAADAKEAAALAPNAAKAVKNWRAGVRKKPTQKQVNALTELAHRVEVLWQIAYRRLNIAEQEIRRDIPIWGSDDLPV
GGAVKREQIEATLADPNGAYQRLRRVMDAWTAMWFWPLTSDTPPPDLDGWIAALQALLGRSPEIRKKNTGPTFAQAADWNELNQAEELELTFSGTKPAAT
VLGDHPWLAVCEQVARQQGFFHWNLDFATVFVRGGFDLQVGNPPWVRPRSDVDALLAEGDPWWQLAVKPSEEKRAEKRETTLALPGIRDLVLEGTTDIAC
LAEHTGSLAQYPHLQGLQPDLYRCFMEQTWRHVSQRGTIGLISLESHFTDEKAGKLRAATYSRLRQHWEFINELQLFEIQHQKHYGVNIYGISREYVKFT
SAVSMYHPDTAIRSQEHDGSGPEPGFKDPSGDWDLRPHRSRLTTVTDETLATWHTILESSDIPIRETRMVYAVNRSTAAVLDKLAHLPRIGKLDLKFSAG
WHEKNDRTKGYFEPKWGKPTSWDDVILQGSHLFVATPFYKFPNSTMKNKDDWSVGDLEQLHADTIPVTIYKPRGSRTDYNLKYTQWGTGSEHPARDYYRL
GWRCMAANTGERTLVSAIIPPKVAHVDGIFSMGSPDCAPRTIAALAAKLSSIVSDFSVRVAPKSTIRAGAVNRIAIGEDYRLDNLLLLRSLRLNCVVDAY
ESLWNECYTDDFSDDHWTGGLNHNRRDPLGDAGPEWSDGTPLRIAADRRQALLEIDSLMALTLGLTADELCSIYRTQFSVLYGYDRNVYYYDANGRLVPN
SVLTVWRKKGDRMSEEERTAMNPAGNTYTYELPFITLDREHDMRVAYAEFQRRLEERS
>EcoPT54I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAABCC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>WviI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CACRAG]
MALSWNEIKDRALKFTKEWEGESRERAEKDTFWNDFFNVFGISRRRLATFEEPVKKLNNKQGFIDLFWKGTLLVEHKSKGKNLDAAFEQATDYFHGIKEH
ELPKYVLVSDFENFKLYDLDDKKEYEFGIKDFHKNIKLFGFIAGYQKRTFKDEDPVNIKAAELMGKLHDQLEESGYEGHPLEVFLVRLLFCLFADDTGIF
EKDTFKEFIEVKTNEDGSDLGAWLAQYFQVLNTPTDKRLKNLDEHLAAFPYVNGKLFEEPLPIASFNSRMREILLECSSLDWGKISPAIFGSMFQSVMNP
EERRNLGAHYTSEKNILKLIKPLFLDELQAEFKKVKSNKNKLKEFHQKLGSLKFLDPACGCGNFLIITYRELRLLELEILKELYGGQQVIGIDQIMLVDV
DQFYGIEYDEFPARIAEVALWLMDHQMNLRISEAFGMYYARLPLKKSATIVHGNALRTIWTDIVPKSELSYILGNPPFYGKQYQSKEQKEDMALVFNGVK
GAGVLDYVTAWYLKASKLINGTEIKVGFVSTNSIAQGEQTGLLWNELFNNYGIKIHFAHRTFNWSNEARGKAAVHVIIIGFANFDTKKKSIWTYDDIKGE
PLEIAVKNINPYLVEGADLVVLKRRKPICNVSEISFGSMPNDGGNFLLSPEEKDELLSIEPNASQVIKPLLSAHEFLNGKKKYCIWLKGVSPKLLKQLKE
VHKRVENVKQLRLKSSRAATQKLAAFPSLFGEIRQPENDFVLVPLNSSMNRKYIPMGFFDNNHIPNNTCSAIDQATLFEFGVLQSDMHMTWVNYVCGRIK
SDYRYSNEIVYNNYPWPKEPSDKNKKAVETKAQKVLDVRAEFLESSLADLYDPLTMPPKLVKAHQELDKAVDLCYRPQAFNNETARIEFLFDLYNEYTMP
LLKKEKKTKKK
>M.CviQII [EnzType=Type_II_methyltransferase] [RecSeq=RAG]
MEPQYAKNKIFDQFYTKREVAETCVNMLEKMVKLDESVVIEPSAGNGAFLEYLPKHTIAIDLDPKKSGIKQQDFFEYSIEANDKKIVIVGNPPFGICNRL
SINFFNHAAKSCNASVIAFIIPKTWRKRSIVIKLDHNFHKIGDMDIPQKAFDDGKVHVACCFQVWERRDILRIDEPRRYPVDGWVFSTADDADFALKKSC
TRAGKIYELHELTKPPSSYFFIKITSSDKNKIRKVFERCVPDFMKSSLNVCNRESLAQGEVEKIYNHYV
>AspJHL3II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CGCCCAG]
MTSTATAATAAIDATATHAFIQRWQHNTASELATAQSFVMDLCALLGVERPHPTPEQTYMFERPVTFAHGDGSTSAGRIDCYRRGHFVLEAKKLKAGSHT
KGFDDGLLRARSQGEAYARALPAADGRPPFVLVVDVGTVIEVYAEFSKTGGTYTPYPDPRSHRLQLADLARPEVQDRLRRIWTDPDSLNPARISAQVTRD
VAALLAQLAKSLEGHGANFKLNQPPALTQQAPIAPETVAAYLTRCLFSMFAEDVELLPKGAFLGLLQTHRGDPATLQQMLRILWADMDRGGFSAALAKPV
LHFNGKLFKGAGQDGYSLRLTPEQIDLLIAAAKSNWREVEPAIFGTLLERALDPTERHALGAHYTPRAYVERLVLPTVIEPLRADWANAQAAALVLAHEA
AALEGKAAQAKLAEARAEVKKFHHQLCTTRVLDPACGSANFLYVTLEHLKRLEGEVVNQLEALGHMQDQLGFEGETVTLQQLRGIELNERAAALAELVLW
IGYLQWHIRTRGNAAVAEPVVHNYGNIECRDAVLAWDTQELAYNDAGQLLSRWDGTTFKTHPVTGEQVPDEAAQVPQWRYVGARQAQWPQADFIVGNPPF
IGAASMRAALGDGYVQALRGAWPEVPESADFVMFWWHHAAAQVAAGHTRRMGLITTNSLRQTFNRRVVEARLSPPPTKATIQTVQPLHLVMAIPDHPWVD
GANGAAVRIAMTVAAPGVGEGQLLTVTDERPGEHGEVTVALQERRGLIHADLSVGANVAAAIPLQAMARISSPGVKLHGAGFIVTPEQAADWNNPALVRH
YRNGRDLTDRPRGSMVIDAFGLTAEELRTQHPSVYQWLLERVKPERDQNNRAVYRNNWWVFGEPRRELRPALAGLPRYIATVETAKHRTFQFLDASILPD
NKLIAITLNDAFHLGLLSSQLHIDWALATGSWLGVGNDPVYVKSRCFETFPFPDEDTGLTPPLRERIAHLAEQIDAHRKRVLGQAGTAGGDFASEARMSP
PQSPPAPALPATTPAASPASAALTLTGLYNVLAALREGRALTPKEKTIHTQGLVGVLRELHDELDAAVLAAYGLPATASTDDILAHLVQLNTQRAAEEAQ
GRVRWLRPAFQNPQNSLQKQELLPQEDKAPEADLDSEKSLSKPEQTKPTQHPWPPTLPEQVRAVAAVLAASPAPLTLPAIEARFKGRGPWKKSLPTLLQT
LEALGRAQAVATDGEVAWRG
>BalI [EnzType=Type_II_restriction_enzyme] [RecSeq=TGGCCA]
MDYAFRDRPLDDVELEVLRLVLSSFRDGSGQVVRPNGGTMPGFRDYERGLAAVLHASAPENKGVFDVIVPVDGDKSFGISCKMATTPPAKHASSFMELSN
SAAQFRQALLAQQINWATEPGLAGPAIVRLVTGWHDATADTHQLDLPASKYSVLAHNPSWTQFQLLCFPLDLQIANPVGEVEWLHEGASLNGYIDDGGRR
HRLWQCYMNSGGQLKYYPLLRWADWVTEPFTLELPPVASPILRARDYFNEVWPHGWDDRN
>Rsp008IV [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=ACGCAG]
MTEHRLLDLVDSRDFRTLFIDELGWGNPDHPDLKLEVDDRKYVFKQVAGYMGLRIWHCPVLPPRKVQRVLDELVGKDSHERLIIFTDEQHQDWRWPRRSQ
LGSTNAKLLAHEYIVGDRSTHLSQRLQAIELDFDEELHLVELLNRMRDAFDKEAETASVQAARLMSALYGELEASGIEDHDATLLLARLLFLFFGDDAEM
KGWETGLFQSFIRDRTTENTLHNDLQALFHVLRTSPNTRKLPVGSPFDRFRYINGGLYEESINISPLTSAFRNYLLEACEFDWTIISPAVFGSMFQTVKS
KEARRHGGEHYTTEENILKTIRPLFLDEYTERLEDAWDDKAKLTKLHNELGRLLFLDPACGCGNFLIVAYRELRALELEILKRRRDLDIIDNTDIKISGV
DRAQLSFDVTGDIKVTLDHFFGIEIEEWPARIAETAMLLVDHLANQRMAQEFGYAPDRLPIKIAPTIHHANALRLDWNDVVEASDDVVILGNPPFVGGRT
KDDEQKYDMQEIWGGQYIGDLDYVTCWYVKAVKYYGKLRGRWAFVSTNSICQGIPVPNLWRPILEEGWRCRFAHRSFQWETEAPGGAAVHVSIVGFDRSK
TSPKPVLWTYPEGGRGEGLGTSVSRINPYLIEGPNLLVESRLRPFNSKFEQVNYANKPSDGGNLVVTEEEYEEIVQDPIAAKYLRKFIGAKELLHDLDRW
CLWIDDNDDLEDLNRSPILAERMAGVKDMRNASKAASTRAKAGTPYKFGQIQQPSTDYLAIPKTVSTLRRFLPVAYLSPDIIASDKVYTVPDPDGFLLGV
FSSTMFIVWMKTIGGRLKSDVSFSNKLTYNTFPMPIITSSQRQSITDAASLIVGARKAHRGLSLARMYDPLAMPSDLLAAHDEVDRVIDKIFGRVDVRDE
ADRQKVLFRNYAILTKQEFLL
>XcmI [EnzType=Type_II_restriction_enzyme] [RecSeq=CCANNNNNNNNNTGG]
MPVTPPQDLIDFIDDILSDLLTNNPLATSSEAYVQNHIEFELVRRNHNPKYYLRIGINYHGEKVQHIMVDPLTGKLIGWNPAGDALGTGANAKMLLANYT
IFDRAPGEHMMTDCKVGGGPLAAGQYVRAEFKVRGWLGKTKNLDGKQFQKDLDLMGADKADLLVWCLSETAHCKFRGEGPAHQAGRRTGCQDFAPILLPT
NQIGIAPVTRQVPYRRIETANLPAAQALWINTQNWVVRSRKVTAAPGSLMPGAEHYVTMCWRV
>BsuMI_(BsuMIB) [EnzType=Type_II_restriction_enzyme] [RecSeq=CTCGAG]
MEISKQTSDLLLSLEKKKGTLPKFSVLRSIPRNRIIYGAPGTGKSNYLEREVGKIFGDNPYVFTRVTFFPGYTYGQFIGAYKPVPIYKKLSGEEEIFSSN
FRDKMENFEPMIDYQFVPGPFIDVLIKALKNRYTNFILIIEEINRANAASVFGDIFQLLDRNKNGESDYPVTFGPDIMNYLARNGIKDEMIKLPSNFFIW
ATMNNADQGVLPLDTAFKRRWSFEYLELEKYRKAVDSWKLSLRYKGHNKVIMWNDFRDIINKRLKGKVPEDKLLGPFFLKESELWNQNVFKNKLLYYLKE
DVFKHNPTIDFLNASTFSELIEKYDGSDNIFTFDIDDSSFVSD
>M.HpyHP26VIII [EnzType=Type_II_methyltransferase] 
MQTLFKEVTPKRYVNGNEMKENSSNVLDQYFTKPSVALKCFQKACEIIKKYENLDDFIFLEPSAGDGVFYDLFPKNRRIGIDIEPKRDGFIQCDFLNYKL
PTHQKVICLGNPPFGHRGVMALEFINHARNCDFVCFVLPMFFESQGKGSIKYRVKGLNLLYSERLEKNAFIDFKNKEVDVHCVFQIWSKKYQNKKSEFSW
YKNRHKEPFGEYIKVFTVSLAKNRECGKEWIFNQKVSFYLSSTFYKSTQIVESFEEVKYKSGIAVVFTSANKALNTKLKKLFKEIDWTKYASLATNSCYH
LGKSHIFQALYDHLDSLKNN
>DsaV [EnzType=Type_II_restriction_enzyme] [RecSeq=CCNGG]
LESQNIRDYTEQTHDKEDKSTEFDFHFTLNGKTYGLSAKRTVRERYKQFIKTSQMSQLDIMIDVTLGIDITEDKLKNIRQHGVYVFVADEVYNESPFMKK
NEGIFPATQFTKETLSNLE
>TspRI [EnzType=Type_II_restriction_enzyme] [RecSeq=CASTG]
MKRSEIEELLEIFRCSLLSIPSGPFARRVHQFTLHGYTYPFVEQYGEAALPDPPPVEVTGRASRRHSMLAAVLLAMKGDFLFFFQADPQDPELGSRRGIR
GVYTVKGPPGRAGHTKPLEHPHYGKDYKMHAACPKCGSPFSSLYGACPECGNPLPLPPKPSRFLRKGKEPLPEHVLSVRLPVEPFTVFEREVTDERVYGD
MSSDNILDRALVWIGRHDNAMGAGKGSSVRQLLPEEALRIYKLLLTESDQRLKSLSSPSGLPTGHIPILNPDGTPLECVLTTEDSSKVREEISIHTALSK
EVNNPHSCLYKRLIPKTVPGLQNLWQTHYLEYVSSEFPWGYTGSTSDYVLVFRPRDGSPVRHAVVIEFKRDEVGIAEVMQAWLYMPWVAQLLGMHLGNLV
GQPGRLVEVHLTPVLVGARLVGRGQNRIHVLPRGYDRTVTYYNGAKVRHVVNPPVFWEYSLKPCGSSQNRAEVRFSPIHLNIKTINYIPPIGTSTAEAER
NRAIEEFRRLAKSLSMGIPLL
>Pme5II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GACGAG]
MPLSWNEIRDRAIAFVREWENESSEDAEAKSFWDAFFNVFGIPRRRIAQFEKLVHKASGKNGYIDLFWPGVMLAEHKSRGKDLTIAHDQAIDYFPGLSDK
ELPRYIVVSDFARFRLYDLEADTEFEFPLEELPQHITAFGFIAGYEARTFREQDPVNIDAAERLGKLHDKLFAIGYEGHQLEVYLVRLLFCLFADDTGIF
MPRDAFEDFISTRTSEDGSDLASRLNELFEVLNTPPEKRLRNLDEQLAAFPYVNGKLFEEHLRTASFDKEMRDLLLWCCHLDWGTISPAIFGSLFQGIMD
AKVRRNLGAHYTSEKNILKLISALFLDDLRAEYERVKTQPAKLRAFHDKLGSLNFFDPACGCGNFLVITYREIRLLELEVIKRLYKRDQQQLSMDAVAHY
VKVDVDQFHGIEIEEWPAQIARVAMWLIDHQMNLLVGQTFGDALVRIPLIKSANIVNDNALRLDWNDVVPAARCSFVLGNPPFVGAKMMSDDQRADIQYA
LGGIKNLGLLDFVAAWYIKAARYAKPETLCAFVSTNSITQGEQAGVLWPVMLDMGIHIHFAHRTFQWRNEAKGVAAVHCIIVGFSQSEPSKRVIYDYLDI
RGEPQVVVASNINPYLVDAADVVLQNRTNPLCDVPKIGIGNKPIDGGHYLFTTEEMEDFIKKEPESKRWFRRWVGSEEFINGFERWCLWLGECPPHELRA
MPLARERIKLVRETRLASKSPPTRKLAETPTRFHVENMPKGNYLIIPEVSSERRLFVPIGFETPETLASNLVKIMPNAGMYHFGILNSTMHNAWMRNVCG
RLKSDYRYSVGIVYNNYPWPTKLSPAQQQAIESAAEGVLQARALYGDSCLADLYDPLTMPPELIKAHRDLDRAVDQAYVISGGKKAWRSDAERVAFLFEL
YLELTAPGVSAVAKA
>TneI [EnzType=Type_II_restriction_enzyme] [RecSeq=GCWGC]
MSSRGTGRTRASVNDCIASVEEGVGRGLLPKEFLGCEDRRGVRHVEVLCEGAVKGGDIESLGGVQEWIQWLAWSSIVLDEKDYLEAALHALRIAPRLSAT
DYGTARQRDLAQQWTDVIRGLLGEIAFVRWLRERFGIYAELDYGLGSISNYLPSDIKLVNGRRPNLNVSIKTTKLRGIWLDVPYAQIEHSDIFVLVRVGV
TRWHFLAFLKKISVIRDKIFEMSTRLGIADGGLLEELWSEMPEFENIPAYVAGFFDKRVYGDRLKSREEIVLADGVQKKKRFIVNRFVGFWNPSTRELPK
YRGKLLQMLRERYPDLPENVKIEFEGIGDFTRTLHFIVSSGALKRGRGEWERLVEEL
>RshI [EnzType=Type_II_restriction_enzyme] [RecSeq=CGATCG]
MADPEPENVSAEAQAAVTSAEPKTLNAYQAIIVDIFQRFYRPGMEEFEFGREEIYEAAVRTGVKAPKNLGDVIYTFRYRRSLPKAILDCQPTGRFWLILG
AGDARYRFRLSKLCYIEPTPGLLVRKIPDATPEIIAQYALGDEQALLAKVRYNRLIDIFLGITAYSLQNHLRTKIPNYGQIEIDELYVGLDSKGAQFIVP
VQAKGGSDRLGVIQTIQDTIFCRTAERYRHCVARTVSAQFMGDDVIAMFELYFDEHDVSIVQEKHYRLVPAREITGSDLGIYRLS
>M.Hpy57III [EnzType=Type_II_methyltransferase] [RecSeq=TCNNGA]
MQTLFKEITPKRYVNGNEMKENSSNVLDQYFTKPSVALKCFQKACEVIKKYENLDDFIFLEPSAGDGVFYDLFPKNRRIGIDIEPKRDGFIQCDFLNYKL
PAHQKVICLGNPPFGHRGVMALEFINHARNCDFVCFILPMFFESQGKGSIKYRVKGLNLLYSKRLEKNAFIDFKNKEVDVHCVFQIWSKKYQNKKSEFSW
YKNRHKEPFGEYIKVFTVSLAKNRECGKEWIFNQKASFYLSSTFYKSTQIVESFEEVKYKSGIAVVFTSTNKALNTKLKKLFKEIDWTKYASLATNSCYH
LGKSHIFQALYDHLDSLKNN
>M.Hpy99XVIII [EnzType=Type_II_methyltransferase] [RecSeq=TCNNGA]
MQTLFKEITSKCYVNGNEMKENSSNVLDQYFTKPSVALKCFQKACEVIKKYENPDDFIFLEPSAGDGVFYDLFPKDRRIGIDIEPKRDGFIQCDFLNYKL
PVHQKVICLGNPPFGHRGVMALEFINHARNCDFVCFILPMFFESQGKGSIKYRVKGLNLLYSERLEKNAFIDFKNKEVDVHCVFQIWSKKYQNKKSEFSW
YKNRHKEPFSEYIKVFTVSLAKNRECGKEWIFNQKASFYISSTFYKSTQIVENFEEVKYQSGIAVVFTSADKVLNAKLKKLFQEIDWTKYASLATNSCYH
LGKSHIFQALHDHLDSLKGN
>AvaIII [EnzType=Type_II_restriction_enzyme] [RecSeq=ATGCAT]
MPIPQHEFTQNIVAILNSYFPSYGNQILEYSELLQYLNIKTKAANRGSKSRASFANHYAIYVLVEDYLNKEFHLNNGYEDYQGVQYSVLFQRQRELPFGK
KLQNHALNNRLNAEFKKYFPTSVYVPIIRDQVTNRYWINENLLRFTINNFQVNIAEAVKDIIDAYVKARTDAFNDFVNYCQEMMAIQQDSERALAFIRNL
LRPNIDARVFEIVSYAILKEYYGEQQIYWGWSLDEINLESLILYKTGRTNANDGGIDFVMKPLGRFFQVTETIDAGKYFLDIDKVQRYPITFVVKTEQPI
QKILDKIKEQATIRYKVKAIVNRYMMAIEEVINIPELMSRLETILERNRGRNVIEEIVLQSKVEFNVEEEYVNAEDEILEE
>M.PfrJS9IV [EnzType=Type_II_methyltransferase] [RecSeq=ARCCCC]
MTTSRPRVLIEDWLPVAELGIESRRERGAASALPPLSFLHIWWARRPLVASAAVALAGVMPAWTEELAESFPDAPEVRTESAYRGWLLRLVGIWGDPISA
RRIYDAAVASGVRVANPYTYRQAFRNAIPRSDIDLLHALLRRTWGTLPVVADPTAGGGSIPWAASRLGLPVVANDLNGVAASVLKAGVEIPATRGLDLLP
DIGKWGDVLVKRVEKRLKEFFPSGEGEVVATYLFANAVPCPRTGRLVPLLTDKWLRKTAGKEAAVRMVTSVGGIELREPRFEVILGREVDKADASTGTMA
RGKAVSPYDNLVIDGDYIKEAAQSGDMSQLLYAVAVRKPSGERTFRAPNEADLQALQAANRTFDAVKDEWFDAGILPTEEFPEGNDLRPKHYGLDRWVDF
FTPRQALVHGTFGEEFARLVPEVREALGEAADQVLFELALMQGKALNYNSRLGSWHVSKQVMRSVFERHDFAFKWTFAEFEGATALYSWCLDQLTDAYGG
IARLLDETGSAELGTNDRLERQVTVTQGSAASLTLDDGSVTHICMDPPYYNNVMYAELADFFYVWEKRTLGRLVPDYFRDELTDKDNEAVANPARFAAMG
RRKNELADLDYESKMTSIFAESRRVLADDGVLSVMFTHKRAEAWDTLGMGLLQAGFTIETSWPVNTEAETSLHQANMNSAASTIMLVCRKREDRETEHRT
YLDDIEQDIRLAARDAATRFQHDGIDGVDLLLSTYGPTLSVISQNWPVYSSAPDADGRDQLLRPEDALALAREEIVDLRRSRLVGQAAKVDGLTDFVLLA
WDTFGAREFPFDTARLLALAVGGLDVDELERAKIVSKASGKVKLLTPKERLRREADSGLPGVTPEASSFEFVIDAVDTALYIAEVDGQQAAKRFLDRHGY
TSDAGFISTLQGLANAIPRTKVKGAWVLPEAGLIDTLCTLYFDDVVLPEAEEMAAPTDPDENTLFDVE
>ApaLI [EnzType=Type_II_restriction_enzyme] [RecSeq=GTGCAC]
MTTRQRLSAERSQQLTRLLTITKTANMRALMEASELAKVIALVAVDIGKSDEMARAFPVLWPKISPQQEYYATAVDWFTNPDETVTSFDVVDMLDAGTSL
DQDFMTYLKCLTELHKRRRKYGLILQRQPLPTMVQVSPRALMEYGPDFPPEALASWLTWRKFFYDLDNRSAQETGYLFEPILAAAIGGEAKSARERVVRR
TDDPTKGRQVDCWKVLPDGTPLAYELKLRVTIAASGQGRFGEELSFARDCSSSGAKPILVVLDPTENDKLTGLQAAYREVGGAAYVGDAAWAHLEDEAGA
TMASFIERYVRVPVASVSSFERVIEGDATKRSLILQDLQARLDGNELTISLGGHQRLVERHEDQSLAADGDDDSE
>BstEII [EnzType=Type_II_restriction_enzyme] [RecSeq=GGTNACC]
MIKNFRDYQRVAAKYITFIESEFYPDYLDNARFLYGEVLNKFYELVNSSSSSIELLENISKTKDPVRTQLLRIFRKYVSPDTSVEMLKRKQRIPDIIKEF
GTRFRDIKIVRQKIATRNHPDETIMALLYEYKDRGKKGYELTDAFFTWFEQKFPNYEIIGPRGAGKDILLNEVLPGFPSKIPADFLIYRRSDKTPIVVGF
ARYDSDRGGAQEDDRTGGNRDKITEIKKYAAEHNIPLKILFLNDGPGLLLGSMWNDYSALEDYGEGCVMVCTLKMLEERFTIDWLENL
>R1.BbrUI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGCGCC]
MASDVSTSTPRRNWSREETMMAFALYIVLKPRECDDKGLDVQRLASFLHRTPSSVVLKIWNIAAYDANRKARGRVGMKHGSKLDSQVWQWYAEDPDTFME
QCLNLLRHALLQADANNFNPAPLASDRYSPLETATTLLVAEHTPTGEERPITTLQRVNQSYFRNSLLQNYHSTCCITGMQIPKLLIASHIKPWKASSAVE
KTAASNGLLLNAFHDRAFDQGLISIDDDYRIMVNHDKVRHSDINDKWLYNFEGREISLPTISQPSHEFIEYHHKHIFLADAA
>TdeII [EnzType=Type_II_restriction_enzyme] [RecSeq=CTCTTC]
MSRREVKNQTNISRIEGIKPNDAYVAYVCVQCNNLNMINIGQKLLDPREAYETQEWKCERCGFLHSKNNSLSYSNWPEESKKKGSIPVQRFWQAFFRVYT
ENKEAYWKQCNCCGKILPFSAFSKHIGFGPLERQMECRACKGVINAFLNPERTEDQLRESNVRRRVADLFVKKENKSKDDGFIKDLFKRFGSKCFKTKKY
LNIHDRNSWAIDHILPSKYLYPLTKENAALLSVEANSNKRDRWPSEFYTNNELIELATITGADLQLLSNKTPIINPNLTDEDINAGIENYLSVRENSNLE
KRVAEIKKIIIDYQLTDKLSKSNKNLLGLS
>Eco4465II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAAABCC]
MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAERAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLL
KALGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQFPADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRF
VLLLGNRQLLLLDRTKWAQNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSEDLKYALRESIELLGNEAMHYLIDR
GLANYTGNRAVDPDELSRECLRYMYRLLFLFYIEARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGYNGGVKMQS
DLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAMSLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKK
AGEEFNELETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTRSLVKYALKELFKEQIDPISDPHAKADAILNLTVC
EPAMGSAAFLNEAINQLAEAYLFHKQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFVPWFGYQLHCGNSLVGARR
QVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHFLLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNTYR
QQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYWCALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHT
TGDLFAEEGLLREESSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNPPWLKVEWQEAGVLGDYEPEFVLRKLSAS
KLATLRIDTFNQIPALEAAWRSEYEGCEGMQNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAHKGVAGFLHPEGIYDDPKGGQLRAAVYPRLRAHFQFQ
NELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFEHSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTP
AWQARLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDSSQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTL
PDDYLPRTNYIPACDAQEYAKRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYCYSSQNLLLEHSGMCFSLPFDFI
CKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVT
FDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA
>HhaII [EnzType=Type_II_restriction_enzyme] [RecSeq=GANTC]
MSKISNALGRKDGNSGYTRVVGNAELGQLLSRVQATVISNGNELERLITQRCNLIENIDVFIEDTTRGNNVQNGVYLCLKKTFKKSKKYAESVKGIEPDM
LIFIVESYRVCKVIELKDGDAFDTKKSQGEKEHLEKFATLFGAKIPFVTDYYICSFNQNDKKLIMAGFKGVFSLEHILTGKELCQILGISYQEILDIRRR
DTEENFAYLIAEMMKIPEVREEVKKHF
>M.BceD122II [EnzType=Type_II_methyltransferase] [RecSeq=RGATCY]
MIKANKEFPNFVKYMGSKSEIIDFIINGINEIHREGQPICDLFAGSTTLSGALRGQAEVISNDIQQYSAVLSETYLIEYDWSSYPNIDQIIQVVDNRVQG
FHAKFKQFKNICTYNEDITLDEFNRIEEKQRALIQFDDFSQFDNYYLFTKYYSGTYWNYNQCVWIDSVKYVADKVSHIKPLYVAIISSLMYAMAYNSQSS
GHYAQYRDAKDEKSMNDILIYRKKNIKDYFIRKFSEIKETLNGPSSKYKIMSSDYKECLNSLEEGTLVYADPPYGLVHYSRFYHAIETLVKYDYPNVAYK
GRYRDDRHQSPFCISSQVKYAFQELFNLIRERKMDLVLSYSNSNTNTISLIELIVQTYVNLNNIDVEEKRGQAKEFIQKHIEDQFALGEEVDKKIDEIIS
FMDTYFVEKYAVDLMYRLKIKKASHNHSTMGRKEDKQRNVDEILIIAERIY
>M.Hpy30VIII [EnzType=Type_II_methyltransferase] [RecSeq=TCNNGA]
MQTLFKEVTPKRYVNGNEMKENSSNVLDQYFTKPSVALKCFQKACEVIKKYENLDDFIFLEPSAGDGVFYDLFPKNRRIGIDIEPKRDGFIQCDFLNYKL
PTHQKVICLGNPPFGHRGVMALEFINHARNCDFVCFILPMFFESQGKGSIKYRVKGLNLLYSERLEKNAFIDFKNKEVDVHCVFQIWSKKYQNKKSEFSW
YKNRHKEPFGEYIKVFTVSLAKNRECGKEWIFNQKASFYLSSTFYKSTQIVENFEEVKYKSGIAVVFTSTNKALNTKLKKLFKEIDWTKYASLATNSCYH
LGKSHIFQALYDHLDSLKDN
>MaqI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CRTTGAC]
MEAFIAASAAVDEFLKRWKGNTGSERANFQSFMRDLCTLLDLPHPDPGEGDTTQNAYVFERFIASARVDGNTDNRYIDLYRRDCFVLEGKQTGKELASRS
QQNAVNAAVAQAERYIRGLPQEEVEHGRPPFIVIVDVGNAIYTYSEFSRTGGNYVPFPDPRHYEIRLEDLHKPDVQHRLRQLWLEPDQLDPSKHAARVTR
EVSTKLAELAKSLEHNGYDVERVASFLKRCLFTMFAEDVELLPKASFQNLLIDIKDRNPEAFPHAVKALWETMNAGGYSERLMQTIKRFNGGLFKGIDPI
PLNVQQIQLLIDAAKADWRFVEPAIFGTLLERALDPRERHKLGAHYTPRAYVERLVMPTLIEPLREQWGDIRGAAETLLRQGKTDKALQEVQAFHYQLCQ
TRVLDPACGSANFLYVALEHMKRLEGEVLGFISELTQGQGVLESEGLTVDPHQFLGLEINPRAAQIAELVLWIGYLQWHYRLNDRLDLPEPILRDFKNIE
CRDALIEYDSREPELNKNGEPVTIWDGISMKVSPTTGELIPDETGRAKVYRYHNPRRAEWPAAEYIIGNPPYIGARRIRSALGDGYLQALRGVYTDIPEH
VDFVMYWWAKASENMASGKTKAFGLITTNSLRQSFSRKVVEKTLDINSDCSIKFVIPDHPWVDSADGAAVRVTLISVDSNKAPGIVALIRNEEAEGSGAY
KITLDNKSGHITPNLTIGADPGEATCLSSNSSVSCVGYQLTGKGFVLTQSQKEEHENEWPESVIKPLWSGRDITQSPRKNWAIDVCDWGIDALKVSSPSL
YQWLLTRVKPEREQNNRASLKERWWIYGEARNTFRPALIGIETAIATSLTAKHRVFVHLDSNSICDSTTVMFALPGAQYLGVLSSRVHVLWSLFAGGTLE
NRPRYNKTLCFETFPFPKMSSDQSEKISDLAEKIDQVRKGQQAKHPDLTLTGMYNVLEKLRSGEELTNKEKTIHEQGLVSVLRELHDDLDRAVFQAYGWS
DLADKLVGRPGATTPLPDKPAEQAEAEDELLMRLLELNKQRAEEESRGIVRWLRPDYQARDAVQTEVDIAPKAAATKTEASTSKGKASFPKAIPDQLRVL
REALAERSHTTESLAEMFKRKPMKSVEEGLQSLVAVGVAEYDPETQTWHTV
>KasI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGCGCC]
MSVIPCKKDLQLKKLIESYAEALKVEAHKLGEHGLTEAEFYDSGLFRGAIERIRGQFSATMREKRNFVKHVLNYMQDNDYIADWESAGESNRHDYMVTLN
SGRKAAIELKGCLDGNNTNIFDRPPQAEEFVIWSVCTNPGADPQHNVWSGLHTRLSAEIISREQRIDGMVIWDWACGTVGRPCPKIATEPERAVTFGPFK
LPPPCLYLLPSTIPSPRNNPSPRAQQIEDVQLIKAFHDCFGCRSEEVNFVNFDVGYHGKDTVRKTTIIRNGMVERESEMTAIRRS
>BclI [EnzType=Type_II_restriction_enzyme] [RecSeq=TGATCA]
MQPNPKFINKSSAFWAYAKLLSEQLGYSKDGVVISYSEAQARAKLKKLGINVKEGIFKDVLRYLKYRAELLNKHKDYLMDVEEARKYFQVALKQHQQNNY
TCKLPLNKQKNEKKDYAYFTCIINIIAETELRYFANNNGLVYGKDIYFDDNPMNLSYILNFNRELEGIMSRRFDGAFPSTVNPILIWEIKEYYYTTTFGS
RIADGVYETQLDGYEIKTIREETNKNIQHIYFIDDYNTWWNMGKSYLCRIIDMLHMGLVDEVIMGKEVFERWPQILRAVLNQYYK
>ApaI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGGCCC]
MARNVLVERAVDAALERLDAFIEGEKLAKLPDAATRALLDDQLGHGSNSVRLASLFFVFYASVDLAWDCNSIPTGIRGTYGDKRLATQLGLRSITLHNAI
TAFGENLGWKGNVTNSRLQNDVRFDGFGRTLTGLNVEQRTLCADYMAARFAESRKVIAPLPPVADDVLTYARARKLFYSLIALPSEGNVQQFLIAALLFV
HRQRYGYDIRTHHVHASDRFDTTAGDIEELLNGDLVRAYEVTVRPDWKNRMGDFRKKMDGASLRKYTIIASNVNSDDDLAEPADMIRFLTPYGRDIAIVD
IHDFINVFAMELIVDELCRAVTQTYNYLTTPSLCGRADIIDKFNATVEGWLDEVT
>BsaAI [EnzType=Type_II_restriction_enzyme] [RecSeq=YACGTR]
MYNYLLNENADIIYDGKVILTKEQVVEAIIITNTNLKKLNDITKESGVEVFEALGMRNLSGFIGEFFVSSLEQVSNKNLVKNPHQDGYPDLLLVDSPKAA
SYFNSIVEIVDGKLYPKEKSLFSPFKYGGLEVKATCGSTPSAKVMPKPLIGEQRIHILTGLDWKAHHRGTNNLIGIYWDFLDELPTICAVFYRNDLTEDD
WGKIVRPKEGGGRTTSVSIMNSKGVKKMCKNWIAIIDNEDYINAFSNKKWIGYNVKNSSN
>BfuAI [EnzType=Type_II_restriction_enzyme] [RecSeq=ACCTGC]
MSHDLLASISSASIANILTDQSTLFTSETINNLSIYASREGKTSWPFADGVIVIEEEATVKYKMAVEFKRVNEGIHGILTALGQSQAYLKKGYNGTIIII
PEVYNTHEAPGEYLKSVLDLVGEDLPIMIFTYKINGENDLEVNCIRNIDLSTTAIDSDDTTNQTNTISTQWAHLREGSTEPDTFYRYLQMAKRIDLTELN
EPTIEFPIELLNALPNDVDPLKYLSNAPGDTYHDFVWRHFWFTYIINERTLPLFTLEGDLYKVCDASSSLLKNDGLPKYFLVGKSNSPKNKIIGKLNAGT
INEEQAWVEYAQKIKDRAHSFREDIDSSLYHIGMIDEDGKPTSIGYKFVDACERNRNDSINGTPLAIFETAIIQHGELGAFIHYISLASQKIFKDTPLKY
SVIEGNEFKSFNSNNYLKEVEEILANDIKVIRKVSLRGGVGRKPFQAELAVLGFLGFFKKGRNRFKPVVGLDIDWEKVYTALNREI
>M.AspNIH2II [EnzType=Type_II_methyltransferase] [RecSeq=TTCGAA]
MSKEKIEFGDFQTPLELAEIACERLVTLGVSPDVIIEPTCGVGAFVIASVNYFPSTKKVYGIDINSDYLDVLEGALCGHPLQNVVSIEHGDFFRYDWVGK
SSDVDGNLLVLGNLPWVTNTVQSKIDSYNVPEKDNVVGLGGLDAITGKANFDISEWMMIDMLKWSPGKKIDIAIMLKTAVARKILSYVEKHKIGCYYSEI
ITVDARKEFGASVDACFFIMRIDSQVKPSYDYKVFESFSDKTGRLCGHRKGFLVSDIDTFELSSRFVSSPPQKWRSGMKHDASSVMELKQHDGVLVNGYG
DVVDIEVEVVYPLLKGSRVGSGKGWDNRFVIVTQERVGEDTSYIETKYPKAWRYLISHADKLDGRKSVIYKKNPRFSVFGVGEYTFKPWKIAICGLYKKL
SFTLVAPIDNKPVVFDDTVYFLSFDLEQEALDALDKLQSKPVLGAISSMIFWDEKRPIKATILNSIDWTA
>Bbr57III [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GTRAAYG]
MKAVRARIDAALEPNSLAQSDSPRAYRELTEEILRNGGGEQGRAKTAERHAYRWFNRIIALRYMDANEFTGVHVVSGEELDNPNALPAVLSAAKRGEFED
EVFGGVGTKSKVLPTIQALLDGGKPSNDPQGDAYGLLLRSYCDYWHRFLPFMFDDAGAADEILMPADLLAKDSVLRKAVEVMTVADCVGESDEGNVEIIG
WLYQFYISERKDQVMAGFKKSKKAGADEIPAATQLFTPDWIVRYLVQNTVGHLWTQNHPESQLHNTWEYYIDPVGEDAGEILKIDSPEDLTVCDPACGSG
HILAYAFDLLYSIYDEAGYSANEIPGLILEHNLFGMEIDERAANLAAFALTMKARGKYRRFFRKGRQVQPNVQRITPEYFADDEVTKLNDLYHVTFDTDT
WNTYQNADTYGSLIQPPADLVSLLPLAGAVEHSETEGGQAQLSLISDQLRDRANLVLTQTRYLSRQYAAVVANPPYMGSGNMGNELKKFVTDHYKDGKAD
LYSCFYYRYETLTDRNGLFGTVIGDTWMFIKSFEAMRTNLIDCRHIESFIHIRDISNHPDIFGANAAFIVSNETKKKHSIFIKLTQNGSWRKRSEFLQAV
QQKTEGAVFTVNQHDFAQIPGSPIVYWLSDAMRKTFTIGKPLEEIAKPRVGLQTGNNDRFIREWWEVSQSRSSFGCKSHEEAAANGKRWFPYNKGGEFRK
WYGNQEFVVNWEHDGKEIENFRPHSVVRNPDLYFHPSISWSKISSGDPAFRFFPGGFIFDVAGTSIFSSDDLRSTIMGYADSTTAYTQLTAIAPTLNFEV
GQIASLPISERLDGIDCDNVKSLIRYSKSDWNSSETSWDFDGLELLSYLSVGFVDDSLDKGIKGISLVELILKYIARCKCIAEEQRQREIKNNELVADAY
GVRNEVPCDVPIERVSLKRNPAFAYPKNTPAERDELMTRDIIKEIISYAVGCMFGRYSLDKPGLILASQGETLADYHAQIPDPSFEPDSDNVIPVTEDEW
FEDDIVARFRRFLSVALGEQHLEENIACIERVLGKSLRKYFVNDFYDDHVKMYKNRPIYWMYSSRTDKKGAFKALVYLHRYTPATTNNVLSYLRDFTAKL
HAQSERLSQSDKASEVRQGEKLQTAIKECADYERDILYPLATRNLPIDLDDGVLVNYLRMGKALRTIPAIEKKRSTVQSWTWPIHELRE
>M.Hpy299VIII [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MVSNTTLQKNLDAFYTHPKIARFCLDLLKDLIHQNLGLGLNAFHFLEPSAGSGSFVGALKGLGIADCLALDIAPKAQGIQKKDYLLELIEFNKKRIIIGN
PPFGHRGKLALDFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIVPPKIRHNDFI
TYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALRIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>NmeBI [EnzType=Type_II_restriction_enzyme] [RecSeq=GACGC]
MTTARWVIDKHLQNFHILCKFAGILKTNSFISVEDKAKLSEKLEKLDLYHRRNTGKSLDATTHKIKELSFYMFGYRDVCGQVTQKFLFSPLGNLFLKHLD
NNEYIQKIFLTMLWAIPFPHPYIKTDESIQLYPMRLIFKLLSDERLDCKLFSYEYIYLISFVKSADQNSYEKLVQDILVLRTCAEVKIKHQLTAENSRSH
AYVNAAHEWESYFSKTLTDAGVLQKTDGKIICRLKHGKTETYRKVTSSEFSIPKQLQEFVKKLQSAYSFSEMPLNLNDSDRLKIDVIKEIYSFYPKELLE
EIGELKDEAAYELLHLPRLIEQYADNNNGTEAYLFEDVLEMGFNMFYNVEAKKIGGPGNTDLECLYITQKRKFAVEAKSTKNKLSGINSGRLEDHKNKIK
AIYTIVVTPRYVPAVLSDIRNCPIVIIRANTFAEFLYNCLINRSSIPEIDYRYFDEIIIKNLGKDYLVQKFPI
>Sth368I [EnzType=Type_II_restriction_enzyme] [RecSeq=GATC]
MAEHIFTEEEVVRILDKIVGKTLGQIDKNNVFDKTKEKPKITGIAGDVIEQCVFGYQADNKQEADLLIDGRPVELKTTGLKKSSPKSKSKHALEAKEPMS
ITAVSVDKIGSQDNFYESTLWHKLEELLIVYYLYDSDKIVKASEYSNFPIQGYQFLIFSEEDKKVLENDWKIVRDFVRNVEKNALDKSIEFPKISKLRTE
MSYMDTAPKYPHPPRFRLTRSYVTTIVQQHFGKDFKPLYPDKEFTSIEGLKDILEKLGKQFIGKSIKEIANELNVPMKLAKNGKVSKSISSIVVAKMFSE
KAQKIEKIALFNKFGIKSKTIVQTRTGGRTEDTKLLMVDFSEITDTTVQFEDSTLYSFFSEQKILFSVFEEVNPDEYESNVFKGFKLISFEDKFIEDTVR
LVWDRTRSLILDKKLKLVVREDKNGQPILNKKGLIEEAPNFPKARECDVFLRGSGKDSTEKTFEINGLRMYPQFYWIKGSYLVKMLNQQIF
>M.Hpy66XII [EnzType=Type_II_methyltransferase] [RecSeq=GTAC]
MNLGAYYTPYYLVDCAYRLLKKHVSIEDYTLLDTACGNKEFLKLHHPKKIGADIDPKCDALIINALANPKRENYGINQDEPLIIVGNPPYNDRTSFIKQN
IKDKDFIFEIDNDLKSRDLGISFLRSFAILKPAFICVLHPLSYLIKEANFKQLKLFKDNYRLLDAFVVSSKSFTKSNEFPIVIALYERGRMDYADIRRFI
FPTDCDTTLCLNDFDYIANYVDKYPNAKKVDACVGYFFPMRDINALKRNKTFLNAPSENAVRISQDKLIYYQYIHYFKEIAPKIPYYFGNLDIIIENSAF
LEVKDAFLKDKRVRLEYFKKLFQGHACEFD
>M.MboBCG21I [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRD
FASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFL
LSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGEL
ADVDVGIVTGRNSFFTFTDAKAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWW
STPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDV
DLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>Bbr57II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAGGAC]
MRRAGRIRAPTAGIPASATSTTGWSSCTCGSMKAACSARISINTIASSTIGRAPRWMPTHGRPPSTTGGWTGIGTIRPKRNGSSTGRTRSWILRICRIGG
SRHACGRSRRMMWSISDRHAGNRIRWCPMDDLKSRAAKFAETWAGRGDEKSDTQQYWRDLLDRVLLIPDTSDRQVLWFERRTALDGFIDALMIQARVLVE
QKSLGVDLDRPEPRQGSMVTPVQQAKRYADSLPPSERPTVLITCDFGTFRLYDLEADPLARTPQSEFTLADLPNHISEIGRLFTHENSRVVQQERLSVKA
GRRVARLHDSLAKCFEHPDDPAEHDALAMLTVRLVFCLYAEDANLFKPDALRDYVAASTPDRLGEDLYDLFEVLDTPIGKRRRYLPEPLKAFPYVDGGLF
ADRIDVPPLTGELRDALLDISEGFDWSGISPVIFGSLMEETLSHDERRKGGMHYTSVRNIHRLIDPLFLDGLKSELEQAEAKPVAGGARTNALNRLHDRI
AGLRFLDPACGSGNFLTETYLELRRIENRILADLDRDGQLALDLGDDLNPVRVSIGHFHGIEINGFACAVARTALWIAEQQALDDTESTITGLPRLPFTD
TAHIMQGNALRLDWNTLLPGGQCDYVMGNPPFIGHVTKTAGQTDDLKTVWGRQYDGYLDYATGWYRKAASYLSKTDAAFAFVTTNSITQGQPVGPLFKPL
HADGWHIRFAHRTFAWDAQSTDNAHVHVIIIGLGRKAKPAPVLFEYADINGEPTARTVDNINGYLIDGPDLYVGKRSQKTGPISPLLDVTDSGSMPLDGG
NLLLADREEYAKAMADPIAARFVRPFRMGRELINGTDRWCLWLRDAEPGELRKSSFLKKRVDACAEYRRNAPMKGDAYRHRATPWLFRDDHQPSMNYLAI
PKVFSEDREYMTCDWYTPDIIAGDMVYTSPDRDGLAFAVIESRMFMTWQQTIGGRLESRCRFSNTVIWNNLPLPALDDDTRMALIEAGRNVLAARANHPG
QSLADLYDPDYMPTDLRAAHRELDKVADVAFGAGTWLKDDDDTRLKVLFDGYARLTGEAS
>M.Pdi8503II [EnzType=Type_II_methyltransferase] [RecSeq=CCWGG]
MYKRTIDNRWSFKDADTKEFTHCYHAYPAMMIPQVARALIEEYRPEDGVELLFDPYMGSGTSLVEASIKGINAIGTDLNPLARLMSHVKTTHYDLSCIRD
TFSMMQALFFEYSEDKVKNKNFDNISNYTYWYSRDSLLRLSYIYQVINECVALDFADFFKVPLSETVREVSFTRNGEFKRFRMKEEKIKDFKPDVFRLFE
EKVIRNINGLEEFNSIKYLCNIGIYDFNSTIEIPSDIIQPNSVDMVVTSPPYGDSRTTVAYGQFSRWANEWFNFENAKTLDNLLMGGRVQKEELFETKSI
KSELDEIKSIDPKRNLEVVSFLNDYSCSIKNVASVIKSGGVVCYVVGNRTVKGVQIPLDYFTAEMFEKNGFTHIDTIVREIPNKRMPSKTSPTNVAGKKV
STMCNEYIVILEKK
>Asu14238IV [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CGTRAC]
MNAVEIEEAVSDLANQPFDSQEFAFSFLEAFGNKDTTVKRLREGNTNKSTIVGGVLQRNNIHIAVSDTGKVTDTLTLLRETPETKKSKTQFILATDGQEF
QAENIISGETLVCEYKDFHEYFSFFLELAGISTVKQIRESAFDIKATGRLNKLYIELLQHNSDWASAERRPDLNHFLARLIFCFFAEDTGIFQGDGLFTL
TVQQMSMSDASDTHEIISEMFRAMDTKLKDREKYNTKTWANKFPYVNGELFSGSTDVPKFTKIARSYLIHVGNLDWKKINPDIFGSMIQAVADDEERGML
GMHYTSVPNILKVLNPLFLDDLREHLEHAGDNARKLLNLRRRMARIRVFDPACGSGNFLVIAYKQIREIEYEINKRRGEKNLSSEIPLTNFRGIELRDFS
AEIARLALIIAEYQCDVLYRGNQLALAEFLPLDAENWITKGNALRINWLSVCPPTGTGVKLHGDDLFSTPLDQAEIDFDNEGGETFICGNPPYLGSKKQN
SSQKNDLRNIFEGYIKGWKPLDYVAGWFLKAVEYGKYTDASSAFVSTNSICQGQQVPLLWPLILNNGHEIIFAHTSFKWSNLASQNAGVTVVIVGISNKK
NIKKRIYDIDNNNQTASREVDNINPYLVPGKNTIVESVRNHICGMPEFSRGNSPTDGGFLLLEKSEVSDLKLTTEQTNKFIRPFVGSNELINGFYRYCIW
IDDENIDEASDIKTINERILRVKEFRLESKKKATVEAANWPYRFDERKPLASEPIICVPVTSSENREYLPVGLLQPGTAVSNSAFNMPSTDLWVLSLIAS
RMNLIWVATVCSRMEMRYRFTSTLAWNTFPIPDLTKKNKEDLTACAEEILIARERHFPSSIADLYDTNKTPEDLKQAHHRNDEVLERIFIGRRFRNDTER
LEKLFELYTKMTNDLKKV
>M.HpyG272XIII [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MVSNTTLQKNLDAFYTHPKIARFCLDLLKDLINQNLGLDLSAFHFLEPSAGSGSFVNALKGLGIADCLALDIAPKAQGIQKKDYLLELIGFNKKRIIIGN
PPFGHRGKLALDFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIAPPKIRHNDFI
TYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALMIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>Lmo6905I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=TAGRAG]
MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITRAKSKFDNGEPFVIKNKLHYEEIEGDVVIAIDAIAQSIKEQKSKPRYILVNDYLDIA
AIDTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDNPDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRT
AEDGSNFNEVIKELFAILDINELARNGKDEWLLEFPYVNGKLFGESHVDLTFSKVSRELLIEAGELLNWNEINPDILGAMIQSVASAEDRHVAGMHYTSV
PNIMKVIKPLFLDDLTESFETLKLRSEENELKDITEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLGDLQNNDSM
PLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELLPLKDAGNIVCANSLRVDWDQVTHFSKEDEIYIMGNPPYIGAKKQTPLQKE
ELESALDGNKKYKKIDYIAGWFYKGAKFIAKYNASLAFVTTNSISQGEQVAMIWPDIFKFAQIFFAYGSFKWSNSAKNNAGVTVAIIGLTSKTEHKDKFL
FSEDIKKKVSSIGPYLAEGDEIIVATANSSIAGFPNAYLGSLPLDGGNFIFDETDRNDFIQKYPALSNLVKKYIGSSELIKQEYRYVLWMNEEEYIEHKD
NPVISERVEKVKHFRKNGGQSAQGAVDRSYEFFTKNQREQAISNNRKHSKKEMKTIIIPRVSSENRSVVPMGIVGEDTVVSDSATAVYNAPMWLLGLLES
RMHMVWLRSIGGKLKTDYRYSAGLVYNTFPIQNLSTQRKNEIARVMTEILDLREYEGGTLADLYNKDSMPESLRKKHEELDGIVDRAYQQRPFESDEDRL
GTLLKLYQEMTTND
>Sau3AI [EnzType=Type_II_restriction_enzyme] [RecSeq=GATC]
MESYLTKQAVHNRAKEAVGKSVLELNGGESIKQSKSSVGDAFENWFGKKKDSDSKPDMAEAGVELKATPFKKLKNGKYSSKERLVLNIINYEKVANENFE
TSSFLSKNNTIELAFYEYIKGTPSDNWIIKEAVLYEMHKNPIDYEIIKQDWEIINQYINEGKAHELSEGLTSYLAPCTKGANASSLRNQPYSDIKAKQRA
FSLKSGYMTSILRKYVLGDEKIDSIVKDPFEIKEKSIEDIVFEKFQPYINWSIDKLCEHFSINKGEKGLNYRIASAILNLKGKTTKSKPFPEVEEFEKSS
IVVKTVHFNKKNVNKESMSFGAFKFEELANEEWEDSEGYPSAQWRNFLLETRFLFFVVKEDEDGVDIFKGIKFFSMPEEDINGPVKRMWDDTVKKLKEGV
TLEAVPDKSTKDGWRIKNNFVDKSDDLICHVRPHTNNRDYRGGSNADKLPKKINWINRPDSDDYSDEWMTKQSFWINNDYIKKQVEDLL
>BslI_(BslIB) [EnzType=Type_II_restriction_enzyme] [RecSeq=CCNNNNNNNGG]
MEQQKFPNPRIFEDIDATDFSKHNKKHVTEDFVAENFKDVGWRVYRPFNDTGIDLIAKKFVCPDGHTKWNQNLTKEMTCSECGKSLIEITRFIQVKTREV
KQVKTREAKGEKFFFGYTLKSKDFRTDPRHVFLLYSDFTMDFIILPMYDYLNLFYTNQSLGSTHFSTPSFRQGNNKLNGLSKDKNDNWVWSGVSFNEFVN
EKGMDKLSCPIYDIELESYTKKIQELKFSLFYRYSPGRKNQVSAPTVEFINNHFSIFISLPKEAIASKRKAHLESLRQDLPEDLKKSVNEGYLVKFKGVD
L
>Exi27195I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GCCGAC]
MAVNQAKIFEHLEQLVENPEQDEFIYGFLTAFEFPKSTLSQIRQGGARNVAKEPGHVALKNKLYYQPVADTDDLESAFEQRIADVAVTKNKIRFVLATNF
VRFLAWDTLTKERLDIEFEELPRNYGFFLPLVGLEKAILNSENPADVKAAEKMGKLFDLIKARNDLSKAEDIHALNVFLTRLLFCFFAEDTGIFEKGQFT
SAVKSYTSEDGSDLDQFLMNLFSVMNSKLDSPLRRSLPIHFTTFPYVNGGLFASDERVPELGLKGRRLLLECSAMDWSAINPDIFGSMFQAVIDVGQRSR
LGQHYTSYSNIMKVIQPLFIEPLRSELEKQRTSTNGLNRLLVRLGEIKIFDPACGSGNFLIVAYKELRLLEMDVIRALLQNDPQSFFMSRIHLDQFYGIE
IDDFACEIARLSLWLAEHQLNKQWEEHIGSAPPALPLRSSGKIWSGNSLQKDWQAVCPKGADDEVYVIGNPPFLGTLGRSDEQRVDMQAVFSGFKSLGGL
DFVACWFWKGAQYIQNSRAELALVATNSLCQGEQVATLWPSVFKLGLCIHFAYPTFAWANNARDKAAVHVVIVGLSGRSKTRQLFQQVDGQWHSKLVSNI
SPYLIEGSNIAVSAKTKPMVKNVPQLLFGNKPTDGGHLLMDRRERDMLLKLEPQAAPWIKRVMGADEFLNGKERWCLWLADITNKELQSMPLVLNRVQKV
AEARRKSPDKGAQKMAERPHQFRDLNNPNDYILIPSVSSERRTYVPIGFYDASVISTNLNYILPNGTLYEFAILTSLMHNDWMRLVAGRLESRYRYSATV
VYNSFPWPSVTNEQRNSLIALAKTVLLTRENYPENTLAELYDPLKMPADLLEAHQTLDKAVDRLYRDKPFRDSTERLSCLLERYEALTKA
>Eco1524I [EnzType=Type_II_restriction_enzyme] [RecSeq=AGGCCT]
MTKNTTAFDVFEKCVQAVQAGELIESISAKDKEFHFQNWFQKRLQSLNMHFEGSGRNTYPDFCLVEHTEGYEIKGLAWPGRERDYDSNSQVPTGYHNGRQ
IFYVFGRYPADLSGYVDNGNGRRQYPVVDLVMCHGDFLNADHNYVHKNKNVKGFGSYGDIMIRDRKMYVAPTPFALTEGTTGLMTLILPENFGTDERYEV
VGNLSRVESERLVVGYNFDLRTNELVADLVPNPRAGTQHQFVAYRLKGQATKAVSMTGGSVLQNENKLADEE
>RceI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CATCGAC]
MPQTMSMPPVLTAVTDPVERFPVERFIERWSPSGGAELANYGLFISELCDLIGVPRPDPRTGDDARDSYVLEKPVTIRHTDDSTSSGRIDCYRKDCFVLE
AKQGVDAESQTALPLLGVAGAPAAPSGTRRRGHGVRGSKGWDDAMIRARGQAEKYAKALPEWPPFLIVLDVGHEIQLFAGFSRTGKDYTHFPDASSFRIR
MTDLRRQETRDLLKAVWLDPLSLDPSKRTAKVTREIAERLAVLAKSLEQAGHSAANVATFLMRCLFTMFAEDVELLPKDSFRDLLRNLRGHPQDFVPTMR
VLWKSMNDGDFCGVLTQKVLRFNGGLFKHADALPLTPEQLELLIQAAEANWRDVEPAIFGTLLERALDKTERHKLGAHYTPRAYVERLVLPTVIEPLREE
WEAVKVAAAEQERDAAVALVMEFHRKLCDTRVLDPACGTGNFLYVTMELMKKLEGEVLDFLSITLDVRQDALDLAGHTVDPHQFLGIEVNPRAAAIAELV
LWIGYLQWHFKTRGKVMPAEPVLKDFKNIENRDAILAWDRTEIVRDESGRPVTRWDGVTMKKHPVTGEDVPDETARVELVRYVKPRPAKWPKADYIVGNP
PFIGTKLMRAALGDGYVNQLRETIDHIPDSADYVMYWWDHAAKLLWNGKIFRFGFVTTNSITQTFNRRVLTNRLKTGAQIYIIFAIPDHPWSDADGSAAV
RISMTVVAKGNSAGRLLLPIREEHLGSETPNIEFNEKHGAISADLRIGANIGNATELISNERICGFGMALHGQGFLLSRAKSDELKKFGSQVIRPFLGGK
DLLSAARERYVIDFSGLTEEEAQIANPAAFQHVMLYVKPERDHNRRDSIKKLWWRFAWERPTLREALKGLDRYIATTETSKHRVFQFIEAIYLTDHMGIV
ISSQDALILGALSSWPHRLWAIKAGGTLEDRPRYNKTRCFDPFPFPDPAEELKARIRVLGERLDSFRKERQAAHPDLTLTQMYNVLERLRELDRDPAAKP
LDAKEKAIHEKGLISVLRQIHDDLDRAVFDAYGWPHDLTDEQILERLVALNKERAAEEKRGIIRWLRPEFQAPKTARPVQTAMEVGPEETSAPAAPAAKA
PWPKSLPEQAQQVLAALTALGRPATPTEVARTFRSAPAPRVAEVMATLETMGRAWKVENTDRYAA
>S2.BliDII [EnzType=Type_I_specificity_subunit] 
MNLGDIADIRTGLVLTRKKVEVGNEVKATYNLITLKNITEEGVFNDEPFEVFHSNDLLNNQHFTEAGDVLIRLNYPHTSVYIDETKSGLLVPSYFAIIKV
DQSKFISEYVAWYLNTDSVKKELERSQAGTRIPSTNKSALNSIPIEDIPIFKQQAVIKLWRLHQQEKTLYNRLIEEKEKWFNAITKQIVQGEIREELE
>M.Hpy298VIII [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MVSNTTLQKNLDAFYTHPKIARFCLDLLKDLIHQNLGLGLNAFHFLEPSAGSGSFVGALKGLGIADCLALDIAPKAQGIQKKDYLLELIEFNKKRIIIGN
PPFGHRGKLALDFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIVPPKIRHNDFI
TYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALRIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>M.MtuF1I [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGSWDGV
AGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEV
VLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGELADVDVGIVTGRNSFFTFTDAKAQA
LGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAPRLTV
NAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDG
LGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>BtsCI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGATG]
MKRILYLLTEERPKINIIHQIINLEYKATLHFGAKIVPVMNEENKFTFIYHVKGIEVEGFDAVLIKIVSGHSSFVDYLVFDSNDLKPEKNTITLFDLDQY
ELDLSYYFGKGWIVRIPSPSDLPKYVVEETKTDDHESRNTNAYQRSSKFVFCELYYGKEVKKYMLYDISDGRTLSGTDTHNFGMRMLVTNNVNLVGVPNM
YLPFTDIKEFINEKNRIADNGPSHNVPIRLKLDKEKNVIYISAKLDKGNGKNKNKISNDPNIGAVAIISATLRNLNWKGDIEIINHNLLPSSISSRSNGN
KLLYIMKKLGVRFNNINVNWNNIKNNINYFFYNITSEKIVSIYYHLYVEDKLSNARVIFDNHAGCGKSYFRTLNNKIIPVGKEIPLPDLVIFDSDQNIVK
VIEAEKAENVYNGVEQLSTFDKFIESYINKYYPGAAVECSVITWGKSSNPYVSFYLDKDGSAVFL
>BfuAII [EnzType=Type_II_restriction_enzyme] [RecSeq=GCATGC]
MFLEESLDYYINREIPNEIEELIKQLKSKRALIVVNHIKEHGYVTNEDLLDVYGYGHGPRAVKDAKDRGIPIKTLSIKSPKTGRTIAAYVFDDITKIRNG
ILEGRKPLDKRIKEELSEIHGEKCYICTEPYEIRYLQADHKIPYDVVGENSSYEYELDKYMLLCRSCNRAKSWSCEYCENFLNSKDENICKSCYWASPDS
YNHIAMKNEKRLELVFSDNEIAVYEIFAYQAKRNNISIPELIKEILKRSVE
>M.VbaLP2AI [EnzType=Type_II_methyltransferase] [RecSeq=TTCGAA]
MAASIRPGHRKGRNRVRDSFNLQERPAMSVERKQRIEFGDFQTPDDLAREICAQLKGFGLNPDTIIEPTCGVGSFLVAAADTFTQAKAVYGFEVNRSYLE
QVEKRVSGRADRQRIQLEAADFFKMDWRARVDALPGHVLVIGNFPWVTNAVQGSIGGVNMPKKSNFLGHNGFDAISGKANFDISEWMLLDVLRWLRGRSA
DVAMLVKTAVARKVLAHAERMGEGVQDSFLMSIDAKKHFGAAVDACLLVMRLDPNGARCSDYTVYETLHAKKGKRVGHRHGMTVGDLDTFESFSFLLGSS
PQKWRSGVKHDASSVMEFTRTSGGYVNGAGEVVQLEEQYLFPLLKGSDIGSAKAWREKFVLVTQRAVGEATHKIQDQAPRTWRYLTAHAEQLDARGSTIY
EKNPRFSIFGVGDYAFRPWRIAICGLYKALRFRLVGPIEGRPVMFDDTVYYLSFESERDATETFDHLHSEAATGFLSSLVFWDEKRPIKTSILNALDWSK
LNEQDGEQGQLTLNIAARNTWSHERRPAARLAQRR
>M.SenCer87DndE [EnzType=Type_II_methyltransferase] 
MLPNRMVLSRQTEDQLKKLKGYTGITPNVAARLAFFRSVESEFRYSPERDSKKLDGSLVLDKITWLGETLQATELVLKMLYPQLEQKILIKAWAAHVDDG
IAALRNYRSLKDFSKNI
>Hpy188I [EnzType=Type_II_restriction_enzyme] [RecSeq=TCNGA]
MAKRKSDIILKSVDDLKDEIDYKDFEYKEYFNLLCELVPNNSLEKLEINAIDEKNMKNEGLVYVFVIQGKIFKIGHSITPITKRVQSYNCGKVEYRKNGT
CSTTNYFVLQSLLKINKIVQVYAFFPEQPTYTLFGKTYQDSFSTSKRAENVILENFIKNHNKKPIGCTQT
>SenCer87DptF [EnzType=Type_II_restriction_enzyme] 
MSAITLRKALGVLAKSSSFSVTTVTHRQKDEFDQLKEQLFVKQEIETELQRYLDVAKPGEIIFLCGSSGDGKSEILTRCQSDPRYQRRFSFHLDATHSFA
PRQSAIDALNDLFTNHHQQSSPLLIGINTGMLANFAREGAECHLAIRSAIDSFLSAQQDESRPYRKVNCSFFDFEHYPKFQFNENKNYSSFIKALLDNLT
RDDDNNLFQFIFRRDESVNPDLKEVANFKLLCIPGVQNVLITQLFKARLIKDQFVTTRTLLDFLHHLLMGPGYLFDNLFTGAENDLIKKVSDFDPARLHT
YELDQFVLRYELGLVDAELDDFLAALEPLHIKFDRQCVKPGDATSLIRLFWLLQHESLGNDYHRKFSAFFNESLFERYSEIWHLHRNYTADSEQKRSLNR
FYAFELIAGIQRYANRKAPELSMQKEEFFLGEFGGVKITAPVEVKPDWDAIRNKHTAHPTGFDVYLKVGQNPLPHIHIGLNLFELLDKLNNGYRPNKYDK
NAIVLLDEIVELIAEQAKSSSEIKFYDGRQRVYRAKADDDMITISGMEG
>HhaI [EnzType=Type_II_restriction_enzyme] [RecSeq=GCGC]
MNWKEFEVFCVTYLNKTYGNKFAKKGESDSTTSDILFTGNNPFYIEAKMPHSQCGQFVLIPNRAKYKFDYSPKNKSEINPYTQKIMQFMSENFSEYANLS
TKGKIIPLPESVFVNWIKEYYKSKSVKFFITSNGDFIIFPIEHFEHYFNVSCTYRIKKSGPRHLNSKSLPDFKQALDKKGISYTMRGLELHSDENIHDKR
ISGDDKDFLIKENNGAYHVKILSNTFNANVIIFNIIKK
>ScoA3II [EnzType=Type_IV_methyl-directed_restriction_enzyme] 
MVAGRNGAGKTSLLRCIALALAANPETGRALVPSYVMWRSPFDTRTVGKISADLFLDSGDLSDLNLEGLPEKPGTHARVGITLTPPEEIPKTGRKRSLNL
GSEWTQVIECDDDDEEVDQAIFPTRNWFTAGYGPFRRLSSEIADPRSTRSQLVTPIDPSSVMDRFSTLFHEDATLGEGVSWLVGLHLRSLENEKGAGRLL
QNVLEVLSDGLLPDGYSPVRVSSSGLWVRRQTGKAFPLKEMSDGFRTVTALVLDIIRQLHTFYGTLRMRRSKKGIAIYAPGVILIDEVDAHLHVGWQQRI
GGWLKSHFPRIQFIVSTHSPYICQSADIGGLIRLPGLNENYPPQVVGEQLYKRIVYGSGDDAALSDLFGLDTPYGSEAEKLRSKLIEIEGRVLDGSASKA
EVRQYREMSETLTSSLSARVDEVSGRLGRRN
>BsrI [EnzType=Type_II_restriction_enzyme] [RecSeq=ACTGG]
MNCMRNIRIYSEVKEQGIFFKEVIQSVLEKANVEVVLVNSAMLDYSDVSVISLIRNQKKFDLLVSEVRDKREIPIVMVEFSTAVTTDDHELQRADAMFWA
YKYKIPYLKISPMEKKSQTADDKFGGGRLLSVNDQIIHMYRTDGVMYHIEWESMDNSAYVKNAELYPSCPDCAPELASLFRCLLETIEKCENIEDYYRIL
LDKLGKQKVAVKWGNFREEKTLEQWKHEKFDLLERFSKSSSRMEYDKDKKELKIKVNRYGHAMDPERGILAFWKLVLGDEWKIVAEFQLQRKTLKGRQSY
QSLFDEVSQEEKLMNIASEIIKNGNVISPDKAIEIHKLATSSTMISTIDLGTPERKYITDDSLKGYLQHGLITNIYKNLLYYVDEIRFTDLQRKTIASLT
WNKEIVNDYYKSLMDQLLDKNLRVLPLTSIKNISEDLITWSSKEILINLGYKILAASYPEAQGDRCILVGPTGKKTERKFIDLIAISPKSKGVILLECKD
KLSKSKDDCEKMNDLLNHNYDKVTKLINVLNINNYNYNNIIYTGVAGLIGRKNVDNLPVDFVIKFKYDAKNLKLNWEINSDILGKHSGSFSMEDVAVVRK
RS
>HbaII [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GCCCAG]
MNAIEIEEAISALAEQDFDAYEFPFAFLEAFGNKKTTIERLRKGATNKSDIGGVLQSNHIHIKVANEGEVSTTFKALKDSDATSKAKAKFILATDGKEFQ
SEELISGETIACEYTQFPDHFGFFLSLAGISTVKQIRENSFDIKATSRLNKLYVKLLEENPEWGTQERRDDLNHFMARLIFCFFAEDTDIFNGKDLFTAT
VEQMSARDSSNTHEVIGEIFRAMNTKIANRENANLPRWADAFPYVNGGLFSGSVDTPTFSKIARTYLIHIGNLDWQKINPDIFGSMIQAVADDEERGALG
MHYTSVPNILKVLNPLFLDDLRAKLEEAGDSPIKLLNLRKRMAKIRVFDPACGSGNFLVIAYKQMREIEAEINRRRKENDRKTDIPITNFRGIELRHFSA
EVARLALIIAEYQCDVTYRGQKEALAELLPLDTQNWITCGNALRLDWLSLMDVGGGGVKLTSNDLFETPLEQAEINFENEGGEIYICGNPPYLGSTWQST
AQKSDLKAIFDGRVQSWKSLDYVAGWFMKAADFGQHADCTAAFVSTNSICQGQQVPILWPAIFASGHYINFAHTSFKWANLASHNAGVTVAIIAVSNMSN
QMRRLYSLNADGEAVMKEVENINAYLVAAPDVTVQKASKPLNGCAEMSFGNKPVDGGNLLLSSDEVEALDLTPDQKARFIRRIYGSAEFIQGKERYCLWI
KDKHLEEAQAIPSISSRIEGVRTMRIASRDKSANEMAKRAHQMREMNIGKKHTIGIAAISSENRPYLPCGLLGNDQTVTNKLFALYDAPLWNMALIASRL
HWVWIGTICVRMRTDFSYSNTLGWNTFPVPKLTEKNKADLTRCAEDILLAREAHFPATIADLYKPDDMPENLRLAHVRNDEVLERIYIGRRFKNDTERLE
KLFELYTKMTKNREL
>HgiDII [EnzType=Type_II_restriction_enzyme] [RecSeq=GTCGAC]
MSDSGYELSFSLNTLNHLSEGLYSNILRFYLKLVANAWDADATEVSINIRQDEIVIQDNGIGMSIEDANTKFLRIGHQKREDSANTISGRHVMGRKGIGI
LAIFGIANIAEVYSCKDGVPHGFIIHKGDIERGISSDVTLYRPSSVPQEALSIESGTKIILREIKSSIGNAEKTLRTDLARRFTIINNNCNFSVIINNTP
INDNDRDYLNKVQFLWYLGGESSKYANFFTKLKKSFEITNLGDGMSGMTVKGWIGTVYRPSDIPNEQPYHIYFAHGKMIQEDILIDITDAGLYRQYIIGE
IEADFMDSDDEDDIITSNRQRIKQTDPRYLKLLEYVKIDIMRVIASTWTKLRKEYPSNPKKEEVNDSSLSKDSNSSEKENTNASSDSSTATENASSDSST
ATENASSETNDGEVEDNSFFDDDIPEPSPPPKQEITTAFREMKNLVKNSNIPNQMKNIILYDIQQAAYAYKGTSFKACIVMLGAILEGVMLGTIQRTDVL
EYLIALPQVPKPLSDLGPRNPKFADRTVLAQYIGTTFSFQDCKEIIELCVQGTNKLGVDILQTVRNSIHPGSVLKDMKQLARFNHQSAVGYIAKLHEIIN
LVILWNPPSIP
>Sgr13350III [EnzType=Type_II_restriction_enzyme] [RecSeq=GCTCTTC]
MRRLAQHTRDDAYASKRDYQELHQVRSVRAYSGTPANNRLVLTAEPVDGIASENIAAFGAYIAAETPLKLTARLCWGDSDRAVAFDLATDFTRVGFALGA
DEQGPLSLELYWDGAYALDIWGLDIGPAAIPDGIVLADVLGAGRSIAPETFYLDHAVALNADLEPSSDAAVAEEGRWLHLKKCSYCGRLLPVDMNRLGSL
AFHKHNAKRTHHQNECRSCKKWRINDNFNPSRTVDQLHESSVITRERKVFLQESTILQEIKKRTGEGLKSQVWERFDRRCFNCAKSIRLSQVQLDHTRPL
AYLWPIDEHATCLCAECNNNKKDRFPVDFYSESQLRELSRICGLGYGELVQKSVNMEQLQKIMGDIVTYATTWEPRTFFSVARKVQEIHPEINLGERLQL
LNPEIHEQLRSRAAAVRLTEDE
>RsaI [EnzType=Type_II_restriction_enzyme] [RecSeq=GTAC]
MERRFQLRWDEEELARAFKVTTKDVREYLTDGRRVSFIIERRLMWENPGWKLAPSEGAGYDLLDPEGGMWEVRSITRQGVYFNPSNQVGSGRKFNEDGFQ
LKMSGIKGFILSDIVGFPLVDVYVVPVENVLRWHQARALGANAKVSREKFLRDMVRDIRH
>TfiI [EnzType=Type_II_restriction_enzyme] [RecSeq=GAWTC]
MSAKESAPEKFVLDSYIEHLKEHFQAALSETKSSVENNLRKNLENKRKQWPYSLLLGERELRYLTITSSLESKLGSSLEKAIRDFVKRHLAHWQVPEESG
KKGSGRKKKPDLVIIDTEGERKTVYVFELKVGGNMDNTKIPGEISKLKNVANNVRQEITDCQNLEAYLAILVDDQTVVSRIKNSASSEGVKVIGGREFWG
MLFSVKDNGMLDYVEEKVKDAYKRAAQEVKFSNLFEPNP
>M.HpyFXII [EnzType=Type_II_methyltransferase] [RecSeq=GTAC]
MINPKHLNKRESVNLGAYYTPCYLVDCAYRLLKKHVSIEDYTLLDTACGNQEFLKLHHPKKIGADIDPNCGALIINALANPKRENYGINQDEPLIIVGNP
PYNDRTSFIKQNIKDKDFIFEIDNDLKSRDLGISFLRSFAILKPAFICVLHPLSYLIKEANFKQLKLFKDNYRLLDAFVVSSKSFTKSNEFPIVIALYER
GRMDYADIRRFIFPTDCDTTLCLNDFDYIANYVDKYPNAKKVDACVGYFFPMRDINALKRNKTFLNAPSENAVRISQDKLIYYQYIHYFKEIAPKIPYYF
GNLDIIIENSAFLEIKDAFLKDKRVRLEYFKKLFQGHACEFD
>Bgl2196IV [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GGAGGC]
MTIDAARFQKLLAECRLEQLFNELGWDHARIGPQEIAVNGERYTVTAVAQKRGVTVFQCSPGTDGTIPARAVLLKIEKEVGKLAHEHLLVFVDAAQTTQT
WLWVARAPGQPAATRTHTWHKGQTGESLRQKLEAIVWSLEEEEAITLTDVISGLKRAFDRDRISRSFYDRFKEQHEAFADFIKGLAGTADKAWYASLMLN
RLMFVYFIQRRGFLDGNPDYLVDRLRAVREAAGKGKFHNFYRQFLRRLFHEGLGQPKNERKSDLSGLIGDVPYLNGGLFEVHALEEANPSIDIPDEAFER
LFAFFDGWDWHLDDRPLKSGNEINPDVLGYIFEKYINQKQMGAYYTKEDITEYISKNTVIPHLLEAAQERCKVAFDGEASVWNLLRDAPHRYIYPAVKTG
VIDAAGEIVPESALPDFVSKGMKDAKARMFDKSYNLGEAVFTTATGERGTLPTETWREYVERRTRALELRERLANGEVRTIDALVTLNLDIRQFMQDVVD
NCSSPDLLRAVWQTIVGRVPETAKEKFRHGITILDPTCGSGAFLFAALNILEPLYEGCLDRMEGFVEDAKKLGKTCDADFVKVLAEVDKHPSRRYFVYKS
IILHNLFGVDIMAEAVEICKLRLFLKLVSQVDRAVQLEPLPDIDFNIRCGNTLVGYANEAQFDAAENLASDAEHRNEIKASIADLADLFDRFREQQTKHG
EKLVRSGEKARLREKLGEMALELDRYLSRDYGIDPDRPKNFRTWRETHQPFHWFAEFYGVMREGGFDVLIGNPPYIQLSEIDQYDTKGYSCTDCGNVYPV
VLERCLALNASTGRQGYIVPVSSISTDGYKSLQQKYRSMNVHVSCYDDRPSRLFDGLEHIRLVIFLLSHNAPGWHSTRYHKWTSQERSTLFQRLAYVQAK
ERIVRNAVPKLTSAHELTILDKLSSCSMTVGARALRAGPHSINFSRKVGYFLQVLDFQPRVVDGSGSLRPPSEFKPLVFASPEEKSAALALLNSNLFYWF
MTVFSDCRHLNKREIDAMPFPDVLHGQAQLVNGLDGHSKDLMANLRATADTRTMRFRHDRLEIECIIPKRSKRFIDAIDQDLAKAFRFSDDETDFIINYD
IKYRMSGVDDEA
>S.XorB812II [EnzType=Type_I_specificity_subunit] [RecSeq=GAGNNNNNNCCT]
MAEHFHTLFTTESSIDALKQTLLQLAVMGKLVPQDPNDEPASELLKRIAEEKARLVVEGKINKQKPLAAFDEEAAPFELPAGWKWTSLAQVAVVNPRNAA
ADSLEISFVPMTFIGTRFDGQHAQETRLWAKVKQGFTHFAEGDVGVAKITPCFENSKACVFSNLVNGLGAGTTELHVVRPVCQTLDPRYVLAYLKSPQFL
LVGETKMTGTAGQKRLPKNFVETNPFPLPPLAEQHRIVAKVDELMALCDQLKARLGEARQVHGNLANALIGQALNGEKEPRAEAVYFSAYLISKLASRRT
FGRVAHMKLLFLADSHLGLGLMDGYRRHAAGPLDTSIYRVEERAAKEGLYSTSIEVLKSGQEKVSYHIGANISRAVETAASALGSARKELDRLIALFEGR
KTDDLEAVATLYAVWNDALAGGLHPSDEWLINEFRSNWHEAKERFTPDVLGRWLGWMRDNGLVPTGNSATTKSQAAFLFN
>M.Hpy32XII [EnzType=Type_II_methyltransferase] [RecSeq=GTAC]
MINPKHLNKRESVNLGAYYTPPYLVDYAYKLLKKHVGIEDYTLLDTACGNKEFLKLHHPKKIGADIDPKCDALIINALANPKRENYGISQDEPLIIVGNP
PYNDRTSFIKQDIKNKDFIFEIDNDLKSRDLGISFLRSFAILKPAFICVLHPLSYLIKEANFKQLKLFKDNYRLLDAFVVSSKSFTKSNEFPIVIALYER
GRMDYADIRRFIFPTDCDTTLCLNDFDYIANYVDKYPNAKKVDACVGYFFPMRDINALKRNKTFLNAPSENAVRISQDKLIYYQYIHYFKEIAPKIPYYF
GNLDIIIENSAFLEIKDAFLKDKRVRLEYFKKLFQGHPCEFD
>M.Hpy32V [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MVSNTTLQKNLDAFYTHPKIARFCLDLLKDLINQNLGLGLNAFHFLEPSAGSGSFVGVLERLGIADCLALDIAPKAQGIQKKDYLLELIEFNKKRIIIGN
PPFGHRGKLALNFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIAPPKIRHNDFI
TYIHNNTPYTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALRIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>M.BceJII [EnzType=Type_II_methyltransferase] [RecSeq=GGCC]
MKSVLDPCCGSRMMWFDRQDERAEFGDIRSETVTVTDRSHREDGTRTLTVAPDRIMDFRALPHADGSFKLVVFDPPHLERAGPRSWLRAKYGALDASTWR
DDLRAGFAECFRVLQPAGVLIFKWNETQIPVREILALTDEKPLFGHQSGKRSATHWICFMKGENHVESR
>Cly7489II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=AAAAGRG]
MIEFLENIGDYFSQHFFDEDFPKKVFDKSGYGADHIKEFTSKIAPLSEKYYKFKNDYLNLRRTKDKVKRTHDFHTQLLQALGYINGVRAYDEPVVMDDKK
IIPIRYRFNKNDKPYLYIMEMQPMIQVGEVDADGLYEQSYIKEDWEKVLPDSWDGYDIRPEVIKEALSELFLLPEEERPVYLIMLAGPKIFLIQYEKWKF
DSYLLFDLEELFTVTKTASNKNLLALFYALLAKKQFISETESVLVGLEEDAHKASYGVTVTLKSAVIYAVEALANEAIYYKSSQAKTLEAKKEIVTLLDN
DNFARELKDECLEMVYRLLFLFYAESRADLEILPIKDSTYQKGYSLEMLRDLEMVPLTTDSSRNGYFFSESLWKLFDFLHTGSDLKDGFQMRPLDSPLFD
NASLKQLKGIQFRNKILQEIVVRLSLSERTKNKGRGRISYANLGINQLGSVYESLLSYSGFFAREDLIEVKNAKDKNGTDGTFLVPKTRRDDFKESEILK
HEEYPEQDVIIPKGHFVYRLNGRDRKNSASYYTPEVLTQTTVKYTLKGIIERLKEKQAIVDGVLTGEACADEILKLKILEPAMGAAAFHNEVINQLAVAY
LELKENEEQAAGRKRITPGIYKDELQKVKAYIAANNVYGVDLNPTAVELGKLSLWLNCMHKNMETPFFAHRLGAGNAVVGAWLKVYNDKDCLEEFPVLAN
GKIGKKPIPKPWWDKAPKRVMWNKKGTLSRKQNQIYHFLLPDDNMVPSYGIKLLKNELTEVEQKAFKDWRNEFKKPLTRVELNRLEKISNVIDYLLEEHY
KQQMGMVKDTASVYQVYGQPNPQTTTKGYDEKERLTDSRKDRSAPYFKLKMILDYWCSLWFWDVRDVRLLPNRTEWYNEVESILQIDSNELLADGSSQDI
KDQIRKKARAGTLFDTSDRIDNIEQLAQQHRFFHNEIEFVEVFYERGGFDVIVGNPPWVSITMDEAGILSEKNPEIFIKKMSASQVKKLAPKILENDVKL
NEDFKKEFIWAQSTKQFLGAIQNYPLLQGHRNNLYKSILTNTFDLSSENGSIGLIHPDGIYDNPKGQKLRKTLYKRLDYHFQFQNKLMLFSEIKDKIIYS
INIYSSPSEINFKNINNLFSVSTIVNSFGHSGLGICGGLKEKNVLNNQFEWNTSPHKSRIVQIESKSLKILQELFEPGANYESAKLPSIHSEDILKILEK
LKENGGTLSDIKFFSTDCWNEVNSQNQGVIEKKVHYPTLDNFEFIYSGPHFFTANPFYRNPQIMVNNHNDYDIINLNLIDQNFIPRTIFTRYEDSDLFKN
RVVGLDNWIFTYRLAMGKMIDTMSERTLQSTIIAPYTSHTNSVISIQTKSEFELLQLSGLSFSLVMDFYIKSLGKSNIYDNTIRNLIFGLDDIFLIFLNS
RTLLLNCLSSAYQSLWERNWNEDFKKDSWSKKDTRLKPFNDLTKEWKWETPLRNWFERRQALVEIDVITAMALGLSLEELILMYNVQFPVLQQNEDDTWY
DATGNIVFTVSKGLVGVGLDRSGWNSIKDMKAGETYEHTITKSELYQGKTITYHAPFTKCDRVEDYKTAWAHFENIFKTEEAVV
>Lmo6907II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=TAGRAG]
MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITRAKSKFDNGEPFVIKNKLHYEEIEGDVVIAIDAIAQSIKEQKSKPRYILVNDYLDIA
AIDTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDNPDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRT
AEDGSNFNEVIKELFAILDINELARNGKDEWLLEFPYVNGKLFGESHVDLTFSKVSRELLIEAGELLNWNEINPDILGAMIQSVASAEDRHVAGMHYTSV
PNIMKVIKPLFLDDLTESFETLKLRSEENELKDITEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLGDLQNNDSM
PLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELLPLKDAGNIVCANSLRVDWDQVTHFSKEDEIYIMGNPPYIGAKKQTPLQKE
ELESALDGNKKYKKIDYIAGWFYKGAKFIAKYNASLAFVTTNSISQGEQVAMIWPDIFKFAQIFFAYGSFKWSNSAKNNAGVTVAIIGLTSKTEHKDKFL
FSEDIKKKVSSIGPYLAEGDEIIVATANSSIAGFPNAYLGSLPLDGGNFIFDETDRNDFIQKYPALSNLVKKYIGSSELIKQEYRYVLWMNEEEYIEHKD
NPVISERVEKVKHFRKNGGQSAQGAVDRSYEFFTKNQREQAISNNRKHSKKEMKTIIIPRVSSENRSVVPMGIVGEDTVVSDSATAVYNAPMWLLGLLES
RMHMVWLRSIGGKLKTDYRYSAGLVYNTFPIQNLSTQRKNEIARVMTEILDLREYEGGTLADLYNKDSMPESLRKKHEELDGIVDRAYQQRPFESDEDRL
GTLLKLYQEMTTND
>ApyPI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=ATCGAC]
MLSDPVFDRATIRHKLIEFKIRWRGHIDQWKAENRPATESSHDQQFWGDLLACFGVNARDLYLYQRSAKRASTGHTGKIDMFIPGKVIGEAKSLGIDLDK
AHEQALDYLLGGTIPNSQMPAYVLCSNFETLRITRLNRDYVGDSAEWDVTFDLDEIDEHLEQLAFLADYETSAYHEEEQASLEASRLMVELFRAMNGDEA
DEAVGDEAPTTPEEEDERVMRTSVYLTRILFLLFGDDAGLWDTPHLFTTFVRNETTPESLGPQLNELFRVLNTPEDKRPKRLPGTLAKFPYVNGAIFAEQ
LDPEYFDYAMREALLNACDFDWSKIDVSVFGSLFQLVKSKEARRGDGEHYTSKTNILKTIGPLFLDELRAQADKLVSNPATPVRKLEEFRDSLAAHIFCD
PACGAGNFLLTAYKELRRIETDLIVAIRQRRGETGMSLNIEWEQKLSIGQFYGFELNWWPAKIAETAMFLVDHQANKELANAVGRPPQRLPITITAHIVH
GNALALDWTEALPKAVGETFIFGNPPFIGQDTRTKQQLEEMKAVWRRKNISRLDYVTCWHIKSLDLFSTRNGRFAFVTTNSITQGEQVPLLFGPIFAAGW
RIRFAHRTFSWDSDAPGKASVHCVIVGFDRAHEPRPQLWDYPNVSSAPVAVPVERVINAYLVDGPNVLVQKMTSPISCEIKPAVLGAMAKDGGGLIVEAQ
DVQEALDDPIAAKYLRPYVGSRELVRGLSRWCLWMVDLDPADVQASTFLRSRIEQVRAYRTTSSAPTTRSMAKIPHLFAQRYRPQTDFLCVPSVVSENRP
YFTAADIEEGTVVSSLAFAVEDSDRSQFALISSSMFITWQKMIGGRLESRLRFANTLTWNTFPVPELDEKTRKRIIKAGQKVLAARALHPERSLAEHYNP
LAMTPELVKAHDALDREVDKAMGAARKLTSERQRQELLFANYAKLTNN
>BslI_(BslIA) [EnzType=Type_II_restriction_enzyme] [RecSeq=CCNNNNNNNGG]
MERQLKSIAYAFVANDIDVYIPDGESNCIVVTKLVCKDCGQYWHTSLSECYFCGTLNFYLYECNSCGKKYSLTSSSKSCDTDGCNGKLIKRCSNPECISR
TNEEIQRATDEQGGVFDLNSSFNVSLNHCVTCGSKENYYKTYRIYSYRTEVEPNIEALREFANNNKLNSDEDVIIIKHLVDNVIHYGYIPYSKLDETTEI
TTTFSRFSDLVSELFPVNVPPNVTE
>CalB3II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GRTTRAG]
MVYLEKELRNVLENSILKARKISEDAAEIAIRQLGVEKSSPYEYLSEQERVLRRRLRAHGRQLGDIRDSKEETQEIDRLIEEVAYEHWHRMLFAKFLFEN
NLLMYFEDGDIKNAVSISFEECEEIAKELGFYNGWELAAKLAAKMLPQIFRADSPVFELKFPLEKEMELEKIIYGLPKDIFGASDSLGWVYQFWQKNKKD
EVNKSEVKIGARELPAVTQLFTEPYMVNFLLDNSLGAWWAKKRLTKEDLTKLKTEEELRDRLKIGSLQFDYIRFVKIDEVWSVAGGNTEGWPENLSEFKL
IDPCCGSGHFLVSVFQKLVPIRMYLENITAKEACDKVLSENIHGLEIDQRCVELAAFSIALAAWNYPQSGGFRILPELQIAWCGQSINVKKDDWISLAGE
DSDLKFHLEGLYNLFEHAPVLGSLINPKCFFEKGSIFEKEWNRVNKKLETSMFAVNTGTSELSVLAYGMEKSFKLLSNSYHLVITNVPYLTLKRQDDLLI
KFVSNNYPSAKYDLSTVFIERLLLFLKENRGVFCSVTPSNWTYLKSYQKFRNYILKNTNIISIANLGEGAFESITGEVVKVLLISLSNNFSKESDLFSGI
ECKIGDDKKNILKNSPLKFQSQNEQFNNPDQRILLDTLGTNKYLSEFAYSKRGIVNGDNEKWIRYFWEIEDSERWRFLQTSVKEIEDFTGRDQVIDWSTD
GLGMLRPSLDNPSYKKKGIAVSRMGDLKATLYTGELYDQNTGVIIPYDEKYITALWCFCKSNSYKAAIRQIDKKLNVTSATLIQCQFDEKYWSDIADEMY
PEGLPKAYSEDPTQWIFHGHPIYSTNPLQVAVARLLGFQWPSESDSSLELSDQSILKIQQTEELQQYSDSDGILCIPTVRGEAPASEKLLDILAGAYKGK
NITDITTKLLSKNDHEGKSLESWLRDKFFSQHVKLFNNRPFIWQVWDGLPDGFSVLLNYHKLDRKNLETLIYTYLGDWINKQKNDIVTGVDGAEEKLAAA
ETLKKSLELILNGEQPYDIFVRWKSIDKQPIGWQPDINDGIRLNIRPFMSVPDIGKRGAGILRDKPNINWNKDRGKDTTDSPWYKVFNGERINDYHLSVE
EKLSVREEKR
>M.AvaI [EnzType=Type_II_methyltransferase] [RecSeq=CYCGRG]
MTSFELESPIEIKTDPTDLDQESDSFVQEISRFNKALEQRFRDKMRLHESLSRKIVSFQANKSKPQYRWFKYKEAFSVDLVNQLIFEYEKKSFERILDPF
AGAGTMLFACSDAGIQADGIEVLPIGQEIIEVRKIIQRQFRREDFLRLIEWYKQKPWNQHNNRKYLNRLRITDGAYPPETEASIERFLFSIEKENILVKQ
VLRFALLCILESISYTRKDGQYLRWDKRAFRKSGSDKFDKGKILDFDEAITEQIKLILNDSFDLISNTLFCYGTQRSGINLFNASCLKILPEFEQDFYDC
IITSPPYCNRYDYTRTYALELALLGVGERDIVQLRQDMLSCTVENKEKSLIHNWQEALRILDKQELLQSILRFLERELERKKLNNNGIPRMIKGYFYEMA
CVIIECFRVLKNGSPLFMVNDNVRYAGIDISVDLILSNIAEEIGFNVEKILVLPTGKGNSSQQMGTHGRKTLRKCVYVWRKP
>Csp2014I_(Csp2014IA) [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GGAGGC]
MATPIPQNQPLQHLQIKSRQRVRDLAEVYTHKREVDAMLRLVDDMFPSEEHPENTDRKFLEPACGSGNFLEEILVRKLTHVTVARYGTSARFEHRTLRAL
ASIYAIDIDPQNVDESKDRLRAVINSHLDMELNTKAATAGFADAVEAILETNIIRADSLAEANSIRFVDYQPSRGHAFVRQWSPMVEQPGMLGIFDIEQD
AVPVHYTELALHPTPTDGGTR
>HpyHI [EnzType=Type_II_restriction_enzyme] [RecSeq=CTNAG]
MSSIKDIIQVYNSVVKDIDKDALEQHDRAYGGVIRSAKGKLLEHISEEIVKIAWQNIGANANRLEINSNKIKIPIKDSYIENIANKQVRAYILERKKDYY
YGLSVDKHIFIDNQLVMGIECKAYTENAMLKRILVDFHLLKTLYPNISCYLFQLESQLGGDYSALPETLLGSKPTHSIMSYFESVNLNIVTLLKGERNIN
QPIHKNFKPLDEQILNKTIKLMENELKVYL
>BseYI_(BseYIB) [EnzType=Type_II_restriction_enzyme] [RecSeq=CCCAGC]
MVINHLLLPNLNINNEKAVPEVYKRILEGYLDYLNTALEYESIAMSEVVAGVISELILYNEIKHDWFLIIKDLLEYDELPISYSKNYGEKLYGFNSQWLQ
HTVHATYNHSFIMNLLNKSQFDYSSIILDLVQPDGYIYNKKVSATNPRTRMKSELLMSLAMGLSLIDSSRIPEQCIVKIKTFDKTEFVTAEYFKLFCLKL
LKIDNLETYCNYNDILLERCFTGTGYADFNVQDKVDDYMGTLKRTARDKSVASPLITVYAGEIAEVLGSSTLDLYNSNKEKYIQHLSLNPLDITAYKMRD
LNADFGESITPFEIFSTIILNN
>S.PfrJS2II [EnzType=Type_I_specificity_subunit] [RecSeq=AAGNNNNNNACT]
MFRFAQYSSQRDTGIPWCPKVPSHWDTPRNKTVMVWRAEPVGAFSGEYQLLSLTKLGVVTRDLSTGKGKIPASFENYQRIRSGDMVMCLFDVDETPRTVG
RARQDGMITGAYERFVATGDTLAEYLNWFYIAMDNTKALRPLYRGLRKTIPVEEFLASHVPTPPLNEQAAIVKYLAHANARINKAIAAKRRLIAFLEEQK
VAVVNSVIRADWPRIPIKRALRSVTAGSWGAAVEESNDPVKWCVRVADFDYSSGGVADSPKTYRAISDREFSRRSLRRGDLLLEGSGGGEKTPVGRVVLF
DYDDDAVCSNFLQRLRSSDDVESEFLMLMLRRVHMSGEVRRYIKQTTGIQNLDLHAYLTHRIPMPSVLEQRRVVSDIQSEFREVDTVVSRIHHEIGLLQE
FRVRLVADVVTGQVDVRAVAATLPDFAEAAADVAEEDDPMLGEELAEVVAGDDE
>AquIII [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAGGAG]
MPLSWNEIKSRAIAFSKEWEFEESEKSEAQSFWNDFFQVFGISRKRIATFEKSVNKLGNKKGSIDLLWKGNILVEHKSRGKSLDKAFEQAKDYFPGLKEH
ELPRYILVSDFAQFRLYDLETDQTHEFLLKDFVNYVHLFDFIAGYEQRTYKDEDPVNIHAAELMGKLHDRLREIGYTGHDLEVYLVRLLFCLFADDTGIF
EKGIFEEYLDIHTKEDGSDLAMHLGHIFHVLNTPPEKRLKNLDESLGQFPYVNGKLFEEQLAPAAFDRKMREMLLEACGFNWGKISPAIFGSMFQAAMDQ
QTRRNLGAHYTSEKNIQKVIKPLFLDELHEKFKKAKGSPTALKRLHDELGELHFLDPACGCGNFLIISYRELRDLELLILKELYKKKEGFIDIRLFLKVD
VDQFGGIEYDEFPARVAEVAMWLIDHQMNIKVSNEFGQYFVRLPLKKAARIVNGNALRIDWEEVIPKEKLNYILGNPPFVGSKMMTKDQRADLLSVFESA
KGAGVMDYVSAWYVKAADFIQEKKIKTAFVSTNSISQGEQVGILWGLLFEKYQIKIHFAHRTFKWSNEAKGKAAVYCVIIGFATFNIKGKRLFEYEDIKG
EALEIKVSNINPYLVNGDDLIILRRRQPLCNVPNIGIGNKPIDGGHYLFTTEEKEDFLKLEPKAEKWFRKWLGSREFINKEERWCLWLGDCPPNELKKMP
HALERVKAVKETRLNSNSKPTQKLAQTPTRFHVENMPESEYLLIPKVSSERRNYIPIGFLNQSTLSSDLVFIVGNATLFHFGIFTSVMHMAWVKYVCGRL
KSDYRYSKDIVYNNFPFPQNVTDKQKQTVEKAAQLVLDTRDKYPDSSLADLYDPLTMPPDLMKAHQKLDKAVDLCYRPQAFTSELNRIEFLFNEYEKLIT
PLLQSTKQKKARKNKTS
>SpeI [EnzType=Type_II_restriction_enzyme] [RecSeq=ACTAGT]
MSIDPNKLNSALYAILGGYRGKFSNKVYNGENDEFDILMEIFGISPLLKRESRQYWGRELGMCWPRLVVEICKQTRNDFGSALQIDGGEPCDLIVGGLAI
ETKYRIGSGDAGTLKKFQAYGSLLSSMGYEPVLLIVREDNLGAAITACHAGGWTVITGQRTFDYLRDLTGINIKELLLQRAGKFPVVR
>MwoI [EnzType=Type_II_restriction_enzyme] [RecSeq=GCNNNNNNNGC]
MKNPKFTENQKEIEKEEFEFLQKLNFIIKESLELFNTNLKNSMKFINYITLPIIMASIESKSFNPFSEIIEKHIAFILNSKMNSLGYKFLPLGYSSDLTY
ENDNSIIHIDIKTANLENPSDFKDTVPLGINQSSYPGVLDCKIRGKNIKADCKKIKVYPNIPTTYNNKLTITNALLFIYPDYKEIIDEIREDYIAIRELI
SINLKDILTPIEGSLEEFLNYKPSNEKKRLEPILDNIVRGYFIHDKLRHEFSENVEKDLEEFEKKIIGIAKKLKEREIKPVAILSISIPNGELAPHYDDE
IVSGKSWGSSFRYHYKKSGNSVFKGLDNKASRAVFLHINKEYLPVLKKYFDPITVYELTEKRL
>BseRI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAGGAG]
MNNSEKQVELARECIIASLGLIRGGKVEDVIRHSFTSYLRTMFPDEPSWIKQHIEGSESAVKFSKEGKLRTGFVDNLVDLTAIEYESNITNKTKFENGYG
QVKDYCASLLNKGYDSELILGVLSDTVRWKAYKIKTIVTPANRKFGRDDIELDEIESIDLSLADNLAGKRLIDFLNTYLGRLGSRPLTASSLANDLGFDS
HFCSRHISSLRELVNNAFTQRPEYGEMITNLWCRFVSYLRDKNSVAEFDREMYSDELYILTLAKLVCANIIENRALRSDRDEISAIMQGDFFKVRGIMNL
VEYDYFGWLNEGEFLEKIIPVAQEMQEDLMAYNFSAPPADDLFGQIMAQLASRSQRILLGQEWTPKWLASSIVKQVLEKLPVEEFPKLVDMCCGSGALIV
EAIEQSKAMIKRNKITSQSSIGLDPTNGSSGMLIKSIEATQCLNEIEIDQAEIELLTQAITGFDIDPLAVMLSKISWLLAARDWLEPFGSFEVTIPVYHA
DSLFAITPLSDVIGEEEQEDCYQLQIAEDLIKLPKFLISPQFLNYFDTLIDFGYNIAITIGMIEDRELESFVSATLNDSELEVDSAMIVSTKRFLSSFIS
TVSRLHSEGRNGIWAFILRNSYRPGLVAGQFNGLVSNPPWLALSKIENNPYQQVLKKKAERFGIKPPGPAFLHIEMATTFLLHAVDRYLKSGAVVGCITP
ETVLNGYNHEPFRQLAFSKTANPVNFELNEIWKLEENTFKNKGIVLFGTKSNSSPVLPNPIPGAVVGKNSLSITSFFMNTQGKRSALSDNQTNRDNKASL
SPGSFKQGADIMPRRLLFHEITPIKSAKGIQQVSVKPIEVGVSPLSFIVKDAKKLSDFRINPTVLPSDLFYDVLTSNMLTPFNIVSPVKALLPIRRGSND
KWEPLTEGSLIAKGQRVNLAFKQIFSAMGNKADINTLWNQINTRGKLAQQVIQPGGYLLFTGTSGEKVCSAFLDTQKIDIERLIIDQTLNWATVETLDEA
CYITGLFNSEAINLMIKDFQPEGAFGGRHIHSLPFRVTPRFDSTQPAHQEVVEKTKFLIMEFQGLKHSDPTIEENLLNPNFSTLARRRKLIKDLIKSLPG
YADYELACRNLYGV
>AchA6III [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=AGCCAG]
MMAVNLLGLVESHSYQVLFLAHLRWSRPDMPALAVDVDGAPVTVTNVSSYKGLRVWVCPVLPNASRQAEIDRIIAKKSTDRIVIFHNDEKQVWRWPSRNV
KGGSTSTRLTSHAHVTGTSNPKLVERLQLITLAITEDLNVTQVIERVRQAFDVETEKETKRASKLMASMYDTLAQADCSEHDISVTLARLLFLLFGDDTD
MWTKNLFQEFLIEHTSRDGSDLAERLNELFAHLDTRPADRTEVGQHLAGFKYINGGLFSERITLPKVGQGLRTTILDACSSNWSDISPAIFGSMFQSVRD
DKTRREYGEHYTSETDILKTLNPLFLDELRDEFARAVGHREEYSRLLKLRERLGRIRFLDPACGCGNFIIIAYREMRLLEISVLERIRVKESEQLVSLAA
NQLEFGLEGELDTAGGSRTTLLDPVVRLDNFFGIEIDEWPAKIAETAMFLIDRQCDLQMLERLGFAPERLPIQRQASIISATPENPSGGNALKLDWKKII
APGPDSIIAGNPPFIGMAWMDKAQQNDNRQIFAMLPEAHGERTGRLDYVACWYAKAIDYLRGSQARVAFVSTNSITQGDQARALDPILKRAGVHIDFAHR
TFKWQSEATGGAAVHCVIIGMSATGRPQRVLFDYPQLSGSPVARPVTGINMYLLDSDLPAPVKRRVPFYAHLPKMTKGSQATDGGHLIVENAEDLAPLKA
DPVARKYLRPFMQGRDMLNSTEKWCLWFEGAQLADLQQVPAIATRLEGVANARLQSPTASVQKQAAIPHLFTQRRQPKNRYLALPEVSSEDREYVPMTYL
EADVIAGNKLIHVDACPEWLFGVLQSRIFMVWLRTFAGRLKSDLSISPDLAYCAFPFPDLSADQTEVISSLAERIVETRLELGKPLSVLYKRGQTPSQLV
SLHDKLDAAVADAFELHVEDELEVASELLKRHHILVGTVKAREVSPPLLGAAANA
>RpaTI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GRTGGAG]
MTPAEFIKKWKPVALTERAAAQTHFLDLCKLFEHEDPVSADPTGEWFTFEKGATKTGGGDGFADVWKKNYFAWEYKKKKRDLGVAMNQLVRYAAALENPP
LQVVCDTDRFVIRTAWTNTVPKEYEIELDDLADPEKRKILWAVFHDPEQLRPQQTRTAITKEAADKFSTIALRLQGRGTPEEIAHFVNQLVFCFFASSVK
LLPEGFFPKLLKRAAQKPQHAIDYFNKLFEAMENGGEYDLTDIAHFNGGLFDGRRALKLDEGDIGLLIEAGSLDWGQIDPTIFGTLFERFLDPDKRAQIG
AHYTDPDKILMIVEPVILRPLRAEWDAARAKIAEIAGEANALQQTGFSKQGAKSFDKKITNIRAKAEVIRDQFIERLRGITILDPACGSGNFLYLALQGV
KDIELRANLECEALGLSPRLPVIGPEIVHGLEINELAAELARTTIWIGDIQWRIRNGIYSNPRPILRKLDSIECRDALITKLTDGTYAEAEWPTAEFIVG
NPPFLGDKFMLDRLGVRYTQALREAFLGRVPGGSDLVCYWLEKARAQILSNETFGAGFVATNSIRGGANRTVVDRVTADLDIFCAWADEDWTIEGADVRV
SLICFSSKGRAQLLVELNGQSVARIFSDLTSSATDFTRARSLRSCREVAFIGNQKGGAFDLPGSIARSFLTLPQNPNGNSNADVVKPWINGLDIVRRPRD
YWIIDFTGLQESEAALYEGPFQYILEHVKEYRNEEAHESSKMNWWIHQRPRHALRLAIDGQSRYLATARVAKHRLFIWVDHQVVPDSQVVAIARSDDATF
GILHSSFHESWTLRLCTWLGVGNDPRYTPTTTFETFPFPEGLTPDIPAGDYADDPRAQAIAKAAKRLDELRKAWLNPPDLVRIEPEVVPGYPDRILPKDT
KAASELKKRTLTNLYNARPQWLADAHRDLDAAVAAAYGWPADITEDDALAKLLELNLSRAGASSPPPANKDEG
>M.PenI [EnzType=Type_II_methyltransferase] [RecSeq=GCAGT]
MIDILEDRLPKEILTELLKDHTTEKNIFWASSDYSELGLGFEVNDFIETHSVTGSYGQVIMPRILKTKAQKKKRTIEKAEIFTPAWVCNDMCNAGDERYR
AKDSNFNKTDYVDGKHVWCACAEPIRFAEGVTWQDYILRNCLEITCGEAPYLVSRYDTTSGELIPLSQRIGLLDRKIRIVNENVSNLCDWMTWTLKSFQT
TYGYDWQGDNVLLARENLFYSFLEYYEERWGEFPSIDKQIEIAKIISWNIFQMDGLKMVIPNSCRHGVIDKDDSDLFNEEKMVICEGCKTNNPSKHNGIP
VKIMDWEKHETIEFRSLYAKKQ
>ScoDS2II_(ScoDS2IIA) [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GCTAAT]
MAIKGIGLKDSMNKFVQTIMPLDEEKEIFNQRLVDYLTHLRDNKNESEEYQKNILKNLLEKTLPNNFINTSNRIDLAIYNGKSANSSLGVLFECKSLTNK
SEMMSKEKLNTKAFQEIIAYYLNERLIYKNLEIKKVIITNGLSWFIIEAKEIEKYFFKNKNLIDLVTKFYNNQLSSNKTDFLYSEVIAPEIDKSIEKGIT
IAHFDLRHALKQGDKIEIKKNNLTQLYRFFTAENLLNKEIFTDSNKLNKAFYDELLYLMGLEEIQQGTSKIISRLKPDKRQYFSFVENIINKLELKDVSK
EKQEEIAIQLTVIWMNRILFLKLLESQLVLFNKDDSYRFLTFEKLNSFEEIYNLFFAVLAKKIPDRNNRIQEKYNYIPYLNSSLFEETELEMSRDGIGID
RLPEGDIYIFDKTILKGMDKKRKKGKINFLEYLFEFLDSYDFSTTISHQQKSKNALINASVLGLIFEKINGYKDGSFYTPGHITMYMSKKAIRTTIVEKI
NEHLGWSCNSIEDIKFQIRNIEVAKKVSKAIDNLKICDPAVGSGHFLVSILNEIIALKSELNVLFDNDGNYIGNLIQCYVINDELIIQDMLGNNFIYQAG
NKLSEQIQKAIFDMKRHILENSLFGVDINPSSVNICRLRLWIELLKSSYYYEDKVIQKQVLTTLPNIDINIKVGDSLLYKFDINYEFDMRRKDLKEYLAL
VKKYKSTNNKRIKTDIWEKIEKIKSSFDDIVSSPELKQLKLLEKELKKASQISLFEDDTIERIRYEQVQKQVKEAKKKLGVRLKNPMYSGGWNGEWNFQK
YLEIMENS
>SpoDI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GCGGRAG]
MTPQDFITKWRNTELKERSASQSHFIDLCRLLDIEDPTTADPKGEWFTFEKGASKTSGGEGWADVWRKDCFAWEYKGKRANLDKAFDQLLQYAIALENPP
LLIVSDMDVIRIHTNWTNTVQQVHTLTLDDLKDAANRDKLRNAFLNPDVFKPSKTRQLVTEQAAQNFANLAQRLRERGHDAQQVAHFVNRLVFCMFAEDV
ELLPNKMFERMIKAARPDPASFAIHAKALFAAMKDGGLVGFEKVDWFNGGLFDNDDVLPLEWEDLDDLIRAAHLDWSDIDPSILGTLFERGLDPAKRSQL
GAHYTDRDKIMQIVNPVIVEPLLAEWAEVKAQIEDLIDKAPKATKDKLLSTSQKAARTRALDKAEALHQAFLDRLKAFRVLDPACGSGNFLYIALLELKN
IEHRVNLEAEALGLPRGFPQIGPEVVLGIELSAYAAELARVSVWIGEIQWMRRNGFEAAKNPILRSLKTIENRDAVLNPDGTRADWPKADVVVGNPPFLG
VYKMGEELGEDYTIALRDAWPEMPGAADLVTYWFAKAWSQMQCGDLSRAGLVATNSIRGGANRTVLKPIAEHGGIFDAWSDEAWTVEGAAVRVSMICFGS
KLPSHPKLNGKVVDKILSDLTANAAGFDLTKSSRISENKGVCIRGIETGGPFEFSQADFEALATKPLNPNGLPNTRVIRRILNGNNILKRQPERYAIDFS
DFRTKEEAALFEAVYSWLEQAYESYERKSKRRIVRRQDWWLHRRSGAALKNAVSRLSRFIVTPRVGKHRIFVWLDSNALADSATFIVARDDETTFGILHS
SFHELWSLRMGTFLGVGNDPRYTPSTTFETFPFPEGLTPNIPADEYADAPRAIKIAAAAKRLNEFRENWLNPADLVDRVPEVVSGYPDRILPKNDAAAKE
LKKRTLTNLYNARPAWLDHAHKALDEAVAEAYGWGDDWRAGVLTEDEILARLFKLNQERAAKEKA
>Cba4G11III [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CACGAG]
MNAVEIEAAISDLALQPFDAAEFPFAFLGAFGNKDTALKRLRAGNNNTSDVHGGVLLRNSIHIAVCEAGAVGKTLQALRASPATVKAKAKFILATDGQTL
EAEELGSGETIAGAFPDFPNHFGFLLPLAGISTIKEIKDNPIDVRATGRLNKLYVELLRENPDWAKDERRADMNHFMARLIFCFFAEDTDIFNGEVLFTR
TVDQFSDRDGSNTHEILEAIFRAMNTKIADRAAEGFRPWADQFPYVNGGLFSGSTEVPRFTRMARTYLLHAGNLNWQQINPDIFGSMIQAVADDEERGSL
GMHYTSVPNILKVLNPLFLDELRAQLDAAGDNKVKLLNLRKRLARIRVFDPACGSGNFLVIAYKKMREIEAEINRRRGESHLGSEIPLTNFRGIELRDFP
AEIARLALIIAEFQCDVLYRGQKDALAEFLPLDAQNWIVCGNALRLDWLSICPPTGTDVRLLADDLFGAPFNHVEIDFENEGGETYLCGNPPYKGSQGQT
DDQKADLQAVFSKHTKNWKSLDYVSGWFMKAADYGTVNDAATAFVSTNSICQGRQVATLWPLIHGTGHEISFAHTSFKWANLASHNAGVTVAVVGISSRP
AALRRIFSVADDGSVVVRETSHINAYLTPGANVVVEAQPEPLGKLSAMSFGSMPNDGGHLLLNVQEAETAIRVHGVNANFIRPFLGSEEFIRGKERRCIW
VRDDDLALATDNDWLAQRFDGVRRQRAASGRETTNALAGQPHRFGEVRQTGNETALIVPSVSSESRTFLPVGLLPRGSIVSNLAFALYDAPLWNMALIAS
RLHLVWIGTVCGKMKTDFRYSNTLGWNTFPVPLLTEQNKADLTACAEDILLAREAHFPATIADLYDPETMPENLRYAHERNDEVLERIYIGRRFKNDTER
LEKLFDLYTKMTTAAAKTKPAAKMKRVKKA
>S.PfrJS10I [EnzType=Type_I_specificity_subunit] [RecSeq=AAGNNNNNNACT]
MFRFAQYSSQRDTGIPWCPKVPSHWDTPRNKTVMVWRAEPVGAFSGEYQLLSLTKLGVVTRDLSTGKGKIPASFENYQRIRSGDMVMCLFDVDETPRTVG
RARQDGMITGAYERFVATGDTLAEYLNWFYIAMDNTKALRPLYRGLRKTIPVEEFLASHVPTPPLNEQAAIVKYLAHANARINKAIAAKRRLIAFLEEQK
VAVVNSVIRADWPRIPIKRALRSVTAGSWGAAVEESNDPVKWCVRVADFDYSSGGVADSPKTYRAISDREFSRRSLRRGDLLLEGSGGGEKTPVGRVVLF
DYDDDAVCSNFLQRLRSSDDVESEFLMLMLRRVHMSGEVRRYIKQTTGIQNLDLHAYLTHRIPMPSVLEQRRVVSDIQSEFREVDTVVSRIHHEIGLLQE
FRVRLVADVVTGQVDVRAVAATLPDFAEAAADVAEEDDPMLGEELAEVVAGDDE
>M.Mtu30073I [EnzType=Type_II_methyltransferase] 
MTTFAGKTAASADKVRGGYYTPPTVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGSWDGV
AGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEV
VLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGELADVDVGIVTGRNSFFTFTDAKAQA
LGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAPRLTV
NAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDG
LGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>HpyAII [EnzType=Type_II_restriction_enzyme] [RecSeq=GAAGA]
MPKLEKILLEITQLDPSKECLKFLANRIKSSDYRGLHLSQHNRYDQNKIKTIIQAIFNEVGGDFLQIRTTDMSKRPSNIIGEEIYAKVVDNICKSEMPQD
NSGKKNQVTQDSLRKNLFVDMHRMGLIERYNKNKEPTNPYIQSNIKYISLTPLAIEFLNAQDLLRKNFCYTQALENLLQGFGAECREVMIELDNHYLDIE
EMMFFVTFLNIENFTRSEIIEYVRGYRSLSRIQKEKLKELVQDYCNPNHFNGNKLEKRDYHNWKNQAQQIFSLLEQSVFFETNKERLILKALNEENKQND
KKLKRSIKEKALYFEKHGVKKEKGFELHHIVPLCLARSIEEFDLLDKWENLIYIDAFNHAKISQTQNKHICLYFKNCDVVLSKGLKEEQESLYLTYIENV
LYKLDLQNAMLKYNKDLLHSKNG
>M.HpyUM032VIII [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MVSNTTLQKNLDAFYTHPKIARFCLDLLKDLIHQNLGLGLNAFHFLEPSAGSGSFVGALKGLGIADCLALDIAPKAQGIQKKDYLLELIEFNKKRIIIGN
PPFGHRGKLALDFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIVPPKIRHNDFI
TYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALRIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>R1.BsuMI [EnzType=Type_II_restriction_enzyme] [RecSeq=CTCGAG]
MTFIKRLEDAYETLLGNYPAGVSSTSTSKYNEIRKIVSEAFLIGENEVYVTGTSRRISNLDTRFAQGNQRNKHTRMAVAFISIPSVDDSELDELIIRTRN
SAITTSSKFCNGEERGTIFDGILLFLVFEGETKVYPLAFLVFENDFELKEKAEELIPGIELKEYPRANQSPAQENNKSAKNEDEESAKSYVVFLDIEEDG
SIVEFVEDKDKTYRIGDMIWTASHTNGSSAITRRLEVIEVVENLVVCKIKHKYNEPVDKNSLLKFVNIEQDLISFLDLHPNVQNGSEGFVSGDIVDENAT
TSSDDLPEDFENN
>Rsp008V [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GCCCAT]
MSAVLNADQRRHLDGAIQQARTAAEQAAADALRALAVAEPSRPSYLNDDDNSLRLALREKARQLGDDTSPSGADLTNLVREVAYEQWHRLLFARFLEVNG
LLRHPEFRDTPLTLDDCTDLADDLGEPDGWSVAARFASEILPGVFRLDDPAVQVRFATEHRLELEKILQSLSAEVFVTEDALGWVYQFWQTAEKKRVNES
GVKIGGADLSPVTQLFTENYMVRFLLENSLGAWWALRHPSSPLADSWTNLRRLDDGTPAAGGFGEWPESAAQVAVMDPCCGSGHFLVAMFGMLWRMRAEE
EGLSVAEAQDAVLRENLHGLELDPRCTQIATFNVALEAWKQGGFRDLPAPQIACSGVPVRSVRSEWEVLAGEDDELRKVMVRLHSLFRSADSLGSLIEPR
TTDFDDVLFGRDLSIGTDWEQVRALLRSALNRENVGGAVLGNAADDVAHAAVLLGRSYTLVATNVPYLSRQKMSDELYQFVLEHHATYSGDLAGAMLNRC
RRLVGQSGTVAAVIPDKLLTAPTYTELRRTLLRSNRWVSVALLGPRAFSTTMFDFGVALLSLDPERPSPRATFPMIDTSKAVDFEAQQLQLLEVAIHETS
TEEQLGRPDSRIGVGRNEVGATLAKFANSYYGLGSGDYGRYGRKFWEVATLGDRWVFQQSTVSAHQEYGGREQILLWEQGRGALSREPGAYLRNIQVWGS
LGVMVSLTGSLWCSLYQGDAWDSNAAPIIPKDPSLLPAIWAFCTSGEFAMAVRAIDRSVKVMNNTLLKVPFDVARWTAIADQMFPDGLPTPRSLDATQWL
FDGVVSESSEPLQVAAARLLGYRWPGQQPDNLDEFADNDGIASLQALPGEPDLATRLRELLAAAYGGQWSSTLERSLVTEAGGKNGRLEDWLRDTFFAQH
VKVFGNRPFLWHIWDGRKADGFSVIVNYHRLDHQTLEKLTFTSLGAWIERQKHEANAGRAGADARLAAAEALQEKLKLILDGAPPYDVFVRWKKLHEQSI
GWYPDLDDGVRLNIRPFVKAGVLRVKLDSKIKWAKDRGTNPDNTERLNDLHPSLEERREARRQGEVKP
>DraI [EnzType=Type_II_restriction_enzyme] [RecSeq=TTTAAA]
MSEIDNLVNFILSKDGIGDKSILEKEVIERFSLTRDRSVYYCTDFAIRFSSSKSAAFSNTVLSLSNLRKFDSKPFIVCLITPAKNYLFLANTSFLKKISH
SSQTLTSNNIRGSFNGSDIYKDFDGIPNSPENFEYLFRIHAETTFEENLIRLAEATNDIAPSGKKFVPSPQGEENIYLAPKRASEFIASDNYRQLLQELD
DIVRHYTNEIIIASMINNVNIRGRVIEYLVAGEDDLLRQNIIYKLRNGGTNLPQFKTDNSLGDYSKAFEGFDTETDVKTKIMLLNSNPKAYNLDKILNFL
SSDKSVFLFYFIGIDSDNSLKTCLVTMFNEELLRGTITLRHWAGRNSRGVSQFDGKIINNIILNPSNKIDKAQAREFLTRILSL
>Xsp91II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CGACCAG]
MGAGARAQMSPLAFVKKWKSASLNERQTAQEHFIDLCALVGHPTPTEDDPTGEFFAFEKGASKVGGGRGFADVWKRDHFAWEYKQKNGDLDGALLQLMRY
APALENPPLHVVCDIERFRIHTAWTNTVPTTYEIALDDITDPHNREILHNVFFNPEKLKPTRTRAGLTKDAADKFSTIAWRLQGRGSPEEIAHFVNQLVF
CFFAQSVGLLRGAIFTKLLRRVSQNPGKACDYLNGLFEAMEKGGEFALTDIMHFNGGLFDGRRALPLDEGDIGLLIAADSLDWSLIDPTIFGTLFERFLD
PKKRAQIGAHYTDADKISRIVDPVILRPLRAEWEKARTEISALLEGTRTPPMRKKLRRRMTRDEAAEEVRSRFIERLCELRILDPACGSGNFLYLALQGV
KDIENRANLDCEMLGLKSRLPAVGPEILLGIEINALAAELARTTIWIGDIQWRLRNGIRTHHVPVLRKLKAIEQADALVERVPVPEGEGQMDLLARPEAG
GAFRSTKWPAADFIVGNPPFLGGKLLRQGLGDNAVERLFTLYDGRVPRESDLVCYWFEQALAMVRNGHTQGVGLVATNSIRGGTNRKVLDQIAADSRIFE
AWSDEPWVVDGAAVRVSLVCFGSGENVCHLDGEPVARINADLTAGAVDLTKARRLRENLGVAFMGDTKGGAFDIPGELARAWLQMPLNPNGRSNSDVLRP
WRNGMDTTRRSRDMWIIDFGWEMSEQEASLYEAPFQYVKEHVLPERAKNRRDTYRERWWRHVEPRPGMWRALHGRHRIIVTPEVSRHRVFAWQSTQIVPD
HKLQIIARDDDFIFGLLNSKYHLRWSLGVGSWHGTGNDPRYTISSCFESFPFPDGLTPDLPVARLQRDPRAGFIAQAAKRLDELRNAWLNPADLTSIEPE
VVPGFPDRMLPKDTAAAAILRVRTLTNLYNRPPQWLINAHRELDDAVAAAYGWPDDISDDEALARLLELNLARASAEEARPSARSTKRQPKSDGEHRQTH
MKLPITGKPQSKGNVSLPELPQQSELERAPPRHRRGRRRAS
>Asp114II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=AGCABCC]
MAVSDALVVGEDWISEHYFTTDAAKGSFLARVLERRKEWEDLEKPADGAAPVQTPRTRLRSARPRLEELLAALPAPDGAGASDSNVADGTLALTAATLEA
SAELDALLREVLGFASAEYRLTDCGPVTLVRPVGDEGPAPLALLRARPAAAVEDLLAKDGRTLAGPWEPVDLAEPAAAPIEGAEPVESVSRALSTLMTDE
HGPTFALVLAGRWALIAEKERWPEGRWLAVDVQIVVERHDVRRGGEVERALACLEAASLVPGPEGETWWTATLAESAAHTVGVSKDLREGVRSSIEVIAN
EVVRRRAAAGLAPLPQSQAQPLALQSLRFIYRIIFLLYAEASPELGVLPVGAAEYDAGYGLERLRELCLRELHEESSQSGTHIYESLSLLFALVDQGHNA
RAAGERADEPVDGLVFQPMRADLFRPEATALIDEVGLGNAALLRVLRSLLLTTEGGKAGRGFISYVELGINQLGAVYEGLMSYTGSFAAERLWEVAPGGD
ASKGSWVVARDVMEGLEERDFVTVTDEATGERRNVTYEAGEFVFRLSGRDRQRSASFYTPEVLTRFTVQQALAELLDCDGRTTTAEEVLGLTVCEPALGS
GAFAIEAVRQLAEQYLSRRERELGERVDPQERPRELAKVRAYLALHQVYGVDLNATAVELAEVSLWLDTMVEGLAAPWFGLRLRRGNSLVGAKRALYSVS
QLKKGAWLRNAPVAEPLSAVAEALDDDGARRLGGAVDVSDRVHHFLLPAKGWGSAVEVPTQVRGLVDAERLRALKTWRREVTKAPTKVQVRRLTALARRV
EVLWELSLRRLRVAEAEASRRIDLWGRQVAPDAGSTVSREEIEAYLADEDSAYRRLRLVMDAWCALWYWPLTTDVTPPTLEHWLNALEGILGTAPKDAKG
TNRTLAEASTWAELQDVEAVELGFAGARPVASVCDDPENRWLEVVRAAAAEQGFFHWELDFATVFARGGFDLQVGNPPWVRPNVEVETLLADGDPWWVLT
SKPSLAERKVRLPLTLDRRGIRNIVVDGASETQVLGEFISDATVYPVMEGRPDLYRAFMCQVWEHQSERGISSLIHMETHFTDAKTPALRAATYRHLRRH
WQFINELQLFDIDHHNAYGIHVYGSERTPSFVHATSLYHPDTVLRSLVHDGSGEEPGFKDPHTGTWDLRPHASRIQRVDGSVLRTWQAVTEAEDWRSTPM
VSTVNSAAARTLSTLASYPRISSLGLRFSSGWNETTDFNSGRFTKAWGKASWEDAILQGPHLHVSTPLYKSPNATMKHNQDWTATDFEALPTRALPVTQY
KPAGDRATYDRLYTHWGESSARDHYRIAWRRMAANTGERTLIPAIIPPGAAHVDGVFSAAMPHTASSAAPTMAALASSLLADFQVRSRARNDIRGNDFNL
LPALPLDNPLAHRVAFRALRLNCMTEAYADLWVECWDESFLDDAPILERYDERPVGPVWTPDVPLRRAEDRRNAQAEIDVMVAMMLGVPVEDLCTIYRTQ
FAVLYDYDHGRGQGAYIYDANGRQVPTPVRQAWAKRGRPDSNDAMPLSERTHPHPGSGVNYVYSLPFRTRDRESDFHHIHSHLSR
>HinfI [EnzType=Type_II_restriction_enzyme] [RecSeq=GANTC]
MNDEISDLINQAVSNILINSSSENKLKKLIKTHDVKIHFVPRNYRIFGGILQSMNIQFGNFLEEFMTLLIKSDGRYDILEEYSGKKSNKFQLSTSNDNRI
DQFISFCQRSDSINLDEEFPKLLNEVKNDNDTNLSSISHDIDILFRNKETGVIYYLEVKYNDDHDTGKFVDINRKFIKTYAYLVREFPNTEIKPILFFFN
NKKMKGNIYVPENTNIRRGKSFFDEFLKIKYEDVDSYIRNLSESPDNIKAFDDLYRKIMAMK
>M.PbaD4II [EnzType=Type_II_methyltransferase] [RecSeq=CCWGG]
MSYAKTLPDTDWDFRLENTKTYTHCYHGYPAMMIPQIANRLIKYYGSDAQQLFDPYCGSGTSLVEANVNGINAVGTDLNPLARLIAEAKTALPEIEKIKS
KIDFFSNLFFSSSFKCVEEEKANLPVFPNLHFWFSEEVIEKLSHIKRYIDEIEDRTVSLFFYVAFSETIREASYTRNSEFKLVRIPEAKRKSFSPDVYGI
FLSKLQRNIRGLLEYSEIMQGVNAKTKILSFDTVKTVEPLKSSEIDLVVTSPPYGDSRTTVAYGQFSRLASQWLDLEEPNKVDNNLMGGKRYFSKIHEMG
FEEVDSSIDKIRQIDEKRAIEVLSFYEDYYKSISNISKIIRDGAFCCYVVGNRIVKNTVLPTDEITKAFFEDNGLRHIDTFNRNIPNKRMPSKNSPTNKT
GAKSATMSREFIVVMRKE
>RdeGBII [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=ACCCAG]
MSSVFKRPDYYDRQKAIAHLRDFARFWTQERIQQWRDDNIKNQEKQYAQQFWSDLLSSFGIIPERISLFERNAERTSTGRNGYIDFFMSGIAIGEAKSLG
ENLDAAEDQLFDYLDSISQNEYPKYGMVSDFERIRIIRLDGSEPKVELLTRDIADYYDSFVFLIGKESVSRQEQEEASIVAADLMAELYTSFLGDDTDIP
VGEDAPKDAEEEDERVQQTSILMTRLLFLLYGDDAGLWPADLFYRWVEQETTSSSLGPQLNQLFQVLNTPPARRSHRMSDLMAQFPYVNGGIFKDSLDAE
FFTEESREALLDACRFQWNRISVAIFGAMFQLVKSKEARRAAGEHYTSEKNILKTLEPLFLSEFREEADRLIRNKSTSLKDFDNFLNNLSTHVFCDPACG
GGNFLNLTYAKLREIETDVLVEKRNRGGEFTASLDISIDQRLSINQFYGFEINWWAAKIAETAMFLVDHQANLRLAQALGDAPNRLPIEIAAHIIHDNAL
RLDWKKAIPEPKGKTYIFGNPPFLGDHTRTAEQLADLQHAWGSSVQLSRLDFVTGWHAKALDYYKNRTGEFAYVTTNSITQGDQTSRLFDPIFKAGWVIK
FAHRTFEWDSEAPGKAAVHCVIIGFTRNQQVKPRLWDYEHPKGNAIERKVKTGINAYLVDGANVLVRKQGMPISKNLPPVQYGSKPTDDGNFIISKEEYV
NLQNDPIISKYLRPYIGSAELLRGTERWCLWLVELDPADVAKSPELKSRIDAVKKFRSESKAKSTQEYPYHHLFRQFGIHDQGRFIGIPEVSSSRRKYLP
VAYFDEKVIISNKVYGAPDDGGLLFAIASSSMFITWMKTVGGRLKSDISFSSTVTWNNFPLPLLRDSDRQKIIAAGQKVLEARALQPNVSLASQYDPRAM
KRELHNAHKELDKVVDVAVFGAKQRCTTDKQRLEILFRHYLELMQRQG
>AccIII [EnzType=Type_II_restriction_enzyme] [RecSeq=TCCGGA]
MLPLDKDLQKAKISITDFFEITNRVLDYFPNVINNTVEKGDYLISSSNIAGTIKFLRPINRKLFIQEKKVFNDYFQKLIIVFENIRNKKTVTEEDKIIID
RVIYTIQQSIGIGLDLMVNQNSARKHVGNRFEELIRVIFTEISVSNKRTVLQIPYETDEGQKIYKCENDLIISPFENVESTNKHLDENEIVVSIKTTSKD
RMGKMFIDKILLERFVKHPQKVIGIFLNDVQRKEDNNISFTLVSGLFMVYTKFLTTLEGIYYLDPPPNALKLPYSNHMKRFSDLITEDLEKLFSS
>HphI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGTGA]
MQIYETYWEITNEYGYNTERFVETLKICVEYIDEIKLVNPNYTETDYSSVIYNELQMRLQSSPILQSTRKGFEGKPKNETSIRKSINQLVKSGFINPFLT
GYHSLAKEYLQTKVNKKRNFLFSRIVYESSNFSYAITDKPDIKVRHINFLVNTLIENFEGKLSKNEIIALMLMDLRTYNGNYYPIDELRNFIRLNQNYIS
EFKERKYNQITYLWGLLSKLDEIYQKDEFICLEEDKKRVFGDLEDTQYLRKRDPYLHRLYKHQLQEESAEYCGGIKCMLEKLAYPVLIASHIKPFIQSDD
NEAYDPNNGLLLSRTLDSLFDLKYISFDDNGNMLKSARLSDDVWQYWRNIKLDSVLLNEKRKQYLKFHRELMEKEDSKN
>HpyAV [EnzType=Type_II_restriction_enzyme] [RecSeq=CCTTC]
MKEQSMIDFLKLRDYDIRKTQNARWIDQKCTPDVLSLVADCILEFTQCNIGKSFSIRDIWDSPYTNENVKMIFSKPDLNSDFSMHEYDKFFSQPIKLLAY
SGILFETKTGNRNIYTIQNIELLEYLMQRETNALKFLILYIQKVLMDSGIYPLFDNFLQKQDTESFKQLKDGFTHFTINNTAINNATECFRIFTKIINPL
AFYYGKKGTRKGYLSNTIITKDELNYNRINWRDIGKDKNTTRQEYDLINSKRIANSNYLISKAKKVVKRYNDRFNNSLSEVKQEKEESQATQIHHIFPIQ
DFPIIANYIENLIALTPNQHFIYAHPNNQTRLIDKDFQYICLLAKTTTILNDTQGVYDWNDYIVVLNMGLKTTIFSQVKNEWELLKVIDAFYFDFNKSKD
PSWSYLLDKNDLRAFKLKF
>RpaB5I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CGRGGAC]
MGDSISVPAVEQFIARWQGREGGQERANYVSFLTELIALLGLDKPDPADATHEHNDYVFERAVKKTAEDSASYGRIDLYKRNSFVLEAKQSRIKGGKKEV
RGQYDLLKTEATAATLGRRGADRAWDVLMLNAKRQAEEYARALPASHGWPPFILVCDVGHCIEVYADFSGQGKNYTQFPDRQNFRIYLEDLRDHDVRERL
RKIWSEPTALDPSQQSAKVTRDIAKRLAQVSLALEKQNYPADDVAMFLMRCLFTMFAEDVELLPEKSFKLLLEDCEKNPEAFVHDVGQLWEAMDTGQWAH
ALKTKVKKFNGEFFKSRAALPLGREEIGELRRAAEYDWNEVDPSIFGTLLEQALDPTDRKKLGAHYTPRAYVERLVIATIIEPLREDWRNVQATAETLRG
AGDLAAAAAAVQAYHDRLCETRVLDPACGTGNFLYVSLELMKRLEGEVLEALLDLGGQEALRGLGSHSVDPHQFLGLEINPRAAAIAELVLWIGYLQWHF
RTKGAPPDEPILRAFKNIKVKNAVLDWDGAPLPKIVEGKETYPNPRRPEWPAAEFIVGNPPFIGASFLRARLGDTHAEALWSAHPQMNESADFVMYWWDR
AAELLTRKGTVLRRFGFVTTNSITQVFQRRVIERHFKAKRPISLAMAIPDHPWTKATTDAAAVRIAMSVGETGRGDGLLQIVVNEAHLDSDTPIVELQGR
VGPINSDLTIGTDLTTTVPLRASEGLASRGVTLAGSGFLITSEEAEHFGLGTHEKLKQHIRGLHNGRDLNQTSRRILVLDFLGLSEEEVRRHFPEAYQHL
LRTVKPERETNKRASYRQNWWVFAEPRKEMRPALKDLGRYIGTARTAKHRIFSMLAGHSLPESEVIAVGSDDAFILGVLSSRLHVRWSLSKGGTLEDRPR
YNNSMCFDPFPFPDANPIQKQTIRVIAEELDAHRKRVLAEHPHLTLTGLYNVLERLRAGAVPQAQPSPAGLTRGSTSSRGAAKKDLDGRGTGRQDGASRL
SPGHDDAEMVLTPDEQCIFDDGLVLILKELHDRLDVAVAEAYGWPANLSDDEILARLVALNKQRADEEKRGLVRWLRPDYQIPRFAKGVDKQAAKEEGAQ
IAASLDLGETRQKPSFPTGAVEQTAAVFAALAAASGPLDAKSLAAQFRRTKTTEKKLAEVLASLARLGYVATTDGVSFALRRVA
>MmeII [EnzType=Type_II_restriction_enzyme] [RecSeq=GATC]
MSETNLNQLAWTSLFEKYDIFNQLETHNFFNITSTQINQFREARLMTKFDNTSQLPNIFSKNGIGILPTSRGSYTLGKFNIFHKFEEIPEEVEHYRFCNI
YESLDFNNISSESTAISCASISKILDDFIGEELVSTVSGRMGTSTFEFSLDKFHTKKITVEKAQIEIDGGYEGEKSFVLIEAKNYISDDFIIRQLYYPFR
KWKETIQKEVKNVYLTYSNGVFELREYAFTDIEGYNSIYLVKSKRYAIYNIVINVEIIQQLILATAIEPEPLDTPFPQADSFERVIKLCELINTSEILSK
DEITENYDFDSRQTDYYLNACKYLGLTEKAFKDGGIAACLSSKGKAIFKKDISSRRLDFIKLILAKTVFRKTLELYFNKASMPTKDEVVLIMKESKLNKV
TSEETYSRRASTVLGWTNWIINQIEE
>NmeDI [EnzType=Type_II_restriction_enzyme] [RecSeq=RCCGGY]
MSGQKLTAANLVSAIDSLKKNVNYNYVNSSNTHTIQIVHVEKPEGPIKIKRGDTETSISKQAIWRLADALSTGEPVNVDRVFGASYNFRSALEALLAHTP
EIYWCKLQRIQPGLNKSEVVEGHKHIIWLPDRPHEKGVMKEYETDVVISEIPSNNVVYDALSLPSTHSEIDIDVKRRHVQIQIALSAIGFQLGFRTWIAR
NDQGISYGDKKIADLDGIVQKIESEKLISSFDGAVQAAMNIDCIWFRNGKLMPAVMEVEHSTGVRSGLARMKQLKDLLPPYANTRWVIVAPDEDRDKVFK
EANVPMFKNLDTQYFPYSAVEELYSLCIRRKLTNKAVNEEFLDCFMEKCVM
>CviAII [EnzType=Type_II_restriction_enzyme] [RecSeq=CATG]
MTQKILNPVTGRFVKVDGSTGKKIKTGNIYDVNNILSSKLTKKIFDKIRRNDLAKEERETQQLTKKISKGIFDKIRSENKKYEKNTQKLTKKMVLDIFSK
IYKEDSRKSKTQCVVSEKKDNGGVLLTEDLGKIFEKSICMLYDTPYIGPYKYGNEKPMLLKTRLTKLLDFFPELTHTAAGGALHDFTTKNSRYLSAKTSK
KKDGKVAPQKIGQPTKKKFLEFFNLPPDTSNDDIKLFIKKNIVRILDEYFKYTFDDTIIYYNEVNNIIMLVKTLKKVKFDPNLIEFGCNKPGKSWKESTI
LFYNNKRLGEFQIHTSRSCIKFRWFFENILLLFPDNFEVTIL
>Csp68KII [EnzType=Type_II_restriction_enzyme] [RecSeq=TTCGAA]
MLPLTVKILCTEAEQFSISESQHKESSLYGVTDGKAVGTYLEHKFKRYLKGKYDFIEGSSASGIDFPDLLVDMKVTSIKQPQSSCPYKSARQKIFGLGYS
LIIFVYNKIDDSKEKIAQLNIQNTIYVSAERTADFQMTRGINQILDNEGNQDDIIAFLFDRNLPVDEIEAANLAEEIIHNPPKQGFLTISNALQWRLQYR
RVISLAGQEEGLIDIYRAN
>Tth111I [EnzType=Type_II_restriction_enzyme] [RecSeq=GACNNNGTC]
MANTLEDHITQVLESFKGEEINRVIAIYKPPDLELAIFYSKIISKLSPIIGNVLERSVAKELGVRLKAPYKRQDPEFPDVVVELGKDKRIGFEIKAWYAL
STEAAARFRTSQKELSSGAYEEVYLVVIAWTMSKLFYGKPKIINLFFEKAIEIARTRDQKYHNPPWNIVLEPVDTSARTINLQQKVVIGKKLQEENLPEG
VQAEEELKKLAQDKKIKDYKVYSTQEDYVDFIRNLERVLPYREDSNFGKIDRIPHERLSSFLKNTKKLKLLGLTLKDWIKVMEYISNQEEKSAQKSKKKK
ELEDLVEKALKKLGYPNTY
>M.Bpe1920II [EnzType=Type_II_methyltransferase] [RecSeq=AAGCTT]
MPPPSRKPAAPRRVRRPAAAPVPRLAPVAGPGRLIDEGLSFYEASLAGKAERYGRGETPHTIHVWWARRPHSAMRALVYAALRAPADKRAAARLSELGAT
PVPQPGLLAEAAAELAGQYGGAPRVLDMFGGGGTIAFEAALLGCDAHSIDVNELAVFLQQANLTYGATLDHGLLKQLAHRHGQAVLQRLARRTETLYPLR
GRLASQAEKPIVYLWTYRHACPQCGYAYTLSRRPWLSKKAGKALAMVATPGPDGDGVDMVSLPDGEPPPSRWAGRGATACPRCSATATPGIADSADHCVA
VVTSAGVGKQFALRDDALPAAGLLREQEETLLQRTGLALPATTLPAWSGIVNPALYGMRGHADLFNPRQRLVTLMLLEELLAEHRTLQAAHGADVARFVS
ASLSGLIDQMVDWNCRLSMWIPQNEQVGRAFCGPGVAMLWDYAEVDPVGTGPGNLHAKLERICAGIDALALLRRPVQVQRASAAALPYPDDSFDAIVTDP
PYYDNIFYSVLADFFYAWKKPLIDAIENRPTTPPHVPTANSGHDELVASRQRAGGSARAAHDEYCARLGQALREAARVLKPDGLLAFVYSHASVLGWAAL
VTAFRAAPFTINSVQPLSIERKARPRALASEAVNTCTTFVARRAQAPRPVLARAALQAQFDAILRGGLHRSLLRAGWHEHDAGVALIAQGVALLANCAAV
EDADTAALIVDLAGQVSALLPGFSIKQRASL
>M.PfrJS9III [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MTLFASKAAPTDDKLRGGYYTPTPLAEFVAGWVARAGHRLLEPSCGDGNILEPLSRLSPAAQGIELFPEEAAIARSRTGADVEIGDFFSWFTPRRHGQYD
GVAGNPPYIRFGNWTEPERSIALDLMRSAGMRPSKLTNAWVPFVVASVLACRTGGRIGLVLPAELMQVGYAAQLRQFLIDRCAEISVVAFKRLVFPGILQ
EVVLLLATVGEGPARMQVVEVDDAASLAGFEIGAAEVRAPLHEREKWTKYHLSPAQIEEIRALRQDERLSPMEHFASVDVGVVTGRNSFFVMSSAEAEDN
RVSDLTIPLVSRSAQLDGTIYTKRDHAASTHRTELLDARGINIHSHQGLQQYVRLGEIEGVHEGYKCRIRKDWWVVPSIWQADAFMLRQIHTHPRVIANA
TSATSTDTVHRMRVAEGVDAKKLATAFFNSVTFALSEIVGRSYGGGILELEPSEAEELRLPDPALVTDELVEQVDRLVRSKKIDEALDLVDQALLVDGLG
FTTDEVTLARSVWTTLRDRRNSRAKKKQD
>FspPK15I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GARGAAG]
MSLKLILEKLDLLEKDGLIYFSDIEEDKIKNFANRVKESLIKIKPDACFCINNEPLILFFENPENLEILQKQIWNFNQSPAIIIKQNNQWIVKNGFKLLE
GNKELETLAESNDILDFEYFKIITGETWEKYKSKFENKNRVDYFLLKNIEDARNLLTSKDKGNLHPKIANSLIGRVIFIRYLIDRKVELKSYKILQKEDF
YNLLSESEKAYNFFKQVRNDFNGNLFPLKYEINNILIHENDFVNDNHLELIISLLKGDNLKNGITQVSLFDIYDFSIIPIEFISNVYEKFIGVENQATQG
AYYTPLFLVDYIQQQTVTKYFQDNPSEYNCKVLDPSCGSGIFLVETLRQIITQYQRINPDFHTDESYEKYKNTLKKLLQDNIFGIDKDENAINVAIFSLY
ITLLDYLTPRSIVGFKFPILINSNFFADDFFNKNGDYNITLKNNHFQFILGNPPWATKHNKEKQLFEKYIESRKIEEQSNLEIENREIAEAFLVRVSDFN
FEQCAFIITSKILYKISRKKEKKGVFRKYFLSRFKISQVFELSSVRHQVFDKSNDKAVAPATILFFSKSDNIEENRKNIITHISLKPNLFFETFKLMVIE
KYDYKELLQSYFIDEDWIWKVLVYGNILDYHFIKRLKTNKSIYDYISDPSHFIFGKGISIGGGDKNPIVEHQKIKYSIDSKNKGLKPFDISYSNNFLSDY
EFVHRQRKLELFKNPVLLVGKGISNSFMAKSAISYDDVIYTDAITGIKPLDEKGFSIINILLALFNSELFSYFLVLTNSSIGIEREQSHDKDDKFSVPLI
ESNNIEFKTKINQISKLIIEENEITFETFRKQEIKNEIAVLKNKINNAIYNLYDLSITEKDLVNYNNTVTIPLLKGTDVKKKNVSKKIAYKESVLEDYAQ
VFINHFGNRFNSDNKYFEVEILYSNHTILMKFKVIPQASKEKKKILWKKEGDKELLKNISNLSFENLSNNLFMQKDVKGFEEDFFYIAKPNQYKSWHPAL
SYLDLAEFIDALHNPKKIGNE
>MjaIV [EnzType=Type_II_restriction_enzyme] [RecSeq=GTNNAC]
MVVKLVNNELKILSGKLRDIFREIYNKIKNDENINDRNLDDKVLSLLKDYTISEENLKIKFSEPKDEIYSYEGRRTPYDLLCYGIINGKNFLIFINNKFG
DLKSNTRNDVTTYNNLLRLYLGIKRQRLTSEITINGELVYNRISGNEIVSYGIFVVDKYRRGYKFFLLEEIKDDFYVNPRNNMFQIRYSPNLGDPIDYFA
FVKKLIDAILESLEKSLNSIKTEILVLNSIKIQLINIKEGKHGED
>M.PspCT06II [EnzType=Type_II_methyltransferase] [RecSeq=CCWGG]
MYKRTIDNRWSFKDADTKEFTHCYHAYPAMMIPQVARALIEEYKPEDGVELLFDPYMGSGTSLVEASIKGINAIGTDLNPLARLMSHVKTTHYDLSCIRD
TFSMMQALFFEYSEDKVKNKNFDNISNYTYWYSRDSLLRLSYIYQVINECVALDFADFFKVPLSETVREVSFTRNGEFKRFRMKEEKIKDFKPDVFRLFE
EKVIRNINGLEEFNSIKYPCNIDIYDFNSTIEIPSDIIQPNSVDMVVTSPPYGDSRTTVAYGQFSRWANEWFNFENAKTLDNLLMGGRVQKEELFETKSI
KSELDEIKSIDPKRNLEVVSFLNDYSCSIKNVASVIKSGGVVCYVVGNRTVKGVQIPLDYFTAEMFEKNGFTHIDTIVREIPNKRMPSKTSPTNVAGKKV
STMCNEYIVILEKK
>Csp68KVI [EnzType=Type_II_restriction_enzyme] [RecSeq=CGCG]
MANVKTVLRELSIAYYLYCLINHTVQNDLNPQNFAEVCQKILTNSQDATVTKEINKVKDLDNFKEYRDILINAEKLAKIIVSNRAFNLNKISTINWVGSK
TKKDNNTDLMINSYEFSLKEDSYILRNMGLYYLINCLTGENRKQGLHIFREYALQEFNQWFVYTYEGLIKYLQNNDNEWTYKSNNYESSMILKGKELTLC
YKKKNQSIIKISLPLELQSEEDFNSRMNSKLIEYSFSKWINQHFSTDSQYLYLKKYCSEQAGKNLIKFLKKNLSYNNPKFKRLLQIYPNTYYYAKSTEQG
QYIYKVPSEEEFTDTIQVSQITYQVTKSQLNILTTLLNTTTQKKLILRNELRYSHGQFKGTPEAKLYLASDEKSLESIYLPIYPSNS
>PmeS132I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GACGAG]
MPLSWNEIRDRAIAFVREWENESSEDAEAKSFWDAFFNVFGIPRRRIAQFEKLVHKASGKNGYIDLFWPGVMLAEHKSRGKDLTIAHDQAIDYFPGLSDK
ELPRYIVVSDFARFRLYDLEADTEFEFPLEELPQHITAFGFIAGYEARTFREQDPVNIDAAERLGKLHDKLFAIGYEGHQLEVYLVRLLFCLFADDTGIF
MPRDAFEDFISTRTSEDGSDLASRLNELFEVLNTPPEKRLRNLDEQLAAFPYVNGKLFEEHLRTASFDKEMRDLLLWCCHLDWGTISPAIFGSLFQGIMD
AKVRRNLGAHYTSEKNILKLISALFLDDLRAEYERVKTQPAKLRAFHDKLGSLNFFDPACGCGNFLVITYREIRLLELEVIKRLYKRDQQQLSMDAVAHY
VKVDVDQFHGIEIEEWPAQIARVAMWLIDHQMNLLVGQTFGDALVRIPLIKSANIVNDNALRLDWNDVVPAARCSFVLGNPPFVGAKMMSDDQRADIQYA
LGGIKNLGLLDFVAAWYIKAARYAKPETLCAFVSTNSITQGEQAGVLWPVMLDMGIHIHFAHRTFQWRNEAKGVAAVHCIIVGFSQSEPSKRVIYDYLDI
RGEPQVVVASNINPYLVDAADVVLQNRTNPLCDVPKIGIGNKPIDGGHYLFTTEEMEDFIKKEPESKRWFRRWVGSEEFINGFERWCLWLGECPPHELRA
MPLARERIKLVRETRLASKSPPTRKLAETPTRFHVENMPKGNYLIIPEVSSERRLFVPIGFETPETLASNLVKIMPNAGMYHFGILNSTMHNAWMRNVCG
RLKSDYRYSVGIVYNNYPWPTKLSPAQQQAIESAAEGVLQARALYGDSCLADLYDPLTMPPELIKAHRDLDRAVDQAYVISGGKKAWRSDAERVAFLFEL
YLELTAPGVSAVAKA
>Acc65I [EnzType=Type_II_restriction_enzyme] [RecSeq=GGTACC]
MKLNAENLSIQEQLAEFDQWLTARLDKIKDSEKFNSEINSLCNCITVLSPLLENFSDPSTCTIHSLVNAVIEASNRIVSGSSFGGDEAALNNFYESFFNL
LFLTSGATDNNLKNHFLIKLNEDDITPLIPKRGSIKKQITFKLYEIPTTTKSDFIARTLASCFTGTKYPLLVKTEPFFDLETYFKIFLEEYIKLILDDEE
DLLQLWAICHSFVELSTNPHGSNLGKYLLNSCTIFKVRGSVSASGGHVTESILREKLSNIGLRADIDYNNNDVKIGDDEIIEDGKRKKKTRAYDFIIPYK
IDNWEPKPKLFIQSQFYAGDSGSVSHKVVDQTQSSRVFTLTKYPNAKFVEYLDGAGYYASLRGDLQHMLSFSNTESFFQVKSILLRLRREFQKIDFLTAI
EIQHAVLISKSRTHKDLQNLLIKDNYSIQEIERAIQTNLELGLITKNESDEIVIPTEHICIARRLLILDIAANYSCSITQAEKSSQKYLLVPGNGANKGI
KESKLAELAFDLCKDINITPTEFIEDIEWLLDEGVIKRF
>M.HpyPVIII [EnzType=Type_II_methyltransferase] [RecSeq=TCNNGA]
MQTLFKEVTPKRYVNGNEMKENSSNVLDQYFTKPSVALKCFQKACEVIKKYESLNDFIFLEPSAGDGVFYDLFPKNRRIGIDIEPKRDGFIQCDFLNYKL
PTHQKVICLGNPPFGHRGVMALEFINHARSCDFVCFILPMFFESQGKGSIKYRVKGLNLLYSERLEKNAFIDFKNKEVDVHCVFQIWSKKYQNKKSEFSW
YKNRHKEPFSEYIKVFTVSLAKNRECGKEWIFNQKASFYISSTFYKSTQIVENFEEVKYQSGIAVVFTSADKVLNAKLKKLFKEIDWTKYASLATNSCYH
LGKSHIFQALYDHLDSLRDN
>EsaWC1I [EnzType=Type_II_restriction_enzyme] [RecSeq=GGCC]
YLTADEVEFINEDEVIIRESKNTTRGVLPSMNDIKDGLFKLLLYSQLSELHYEDRRLRFTAQMRLTGNFSGELSLPAKESCLQGFLSQFRSERQRKSIEC
KLTWLNRESELLGIQAILRGDTTSVGGVS
>M.HaeV [EnzType=Type_II_methyltransferase] [RecSeq=BA]
MANQNTFKQAPLPFIGQKRMFLKHFETVLNENIKGDGEGWIIIDTFGGSGLLSHTAKRLKPKARVIYNDFDGYAERLAHIDDINRLRAELYSVVGNATSK
NKRMTKDCKAECIRIIQNFKGYKDLNCLASWLLFSGQQVATLDDLFQHNFWHCIRQSDYPKADGYLDGVEIVKESFHTLLPKFSNDPKALFVLDPPYLCT
KQESYKQATYFDLIDFLRLVNITRPPYVFFSSTKSEFIRFVNYMLEDKVDNWQAFENAKRITVNAKLNYQVAYEDNLVYKF
>M.LmoJ1220I [EnzType=Type_II_methyltransferase] [RecSeq=GTATCC]
FSSAVCFVIAXCTMTLLSPLXRVKTGLICSICCGSVNFRNNRTGAFISFKSSALLIINCSSPFLVSALTCKKRISPFCAKLANLRXLSGNMTALSSFESS
LIVKKHIVSPFFVLIDLTSCTXAATXXFLPEERFASLVIARFCNLFWXYDTGCAETYXPVYNFSFSSFSSIEMGAVFRXRGFIFFKKSSSSAKRFIPFES
SPSSEIYCSTASMVAKIVARSSPNSSKHPENTSASKTVSESILCGILSQKSKKSRKGAFFRSLIISKMNFVGTLRITRKLXRIDDSFSTWKXXLLWLMLG
ANMLIPXFFASVMYWEILSSLPKTELRSDDINAAGKCAFKXANXKAIFEYAAAXLRLKPXFANXTIISYTWLALASEXPLLLAPSTKXIRSSFRTSAFFL
LTARLNKSASPSENPAIWFATXIICSXXTITPYVTSKIGAKSGXGYXIFSFPCFLAINVSICSIGPGRXSASTATTSSKEVGFNFRSTRRIPSDSNWNTP
VVSPVWNSFNVFLSSKEISANVNGVSLVXLTDFKTRSSKLRFLSPKKSILSKPIFSNSPIANWVKRASPLPNCSATCSTSGLSLITIPAACVEIXRGKPS
SXQATSQIISFSLELLIWFSKR
>M.Bpe137II [EnzType=Type_II_methyltransferase] [RecSeq=AAGCTT]
MPPPSRKPAAPRRVRRPAAAPVPRLAPVAGPGRLIDEGLSFYEASLAGKAERYGRGETPHTIHVWWARRPHSAMRALVYAALRAPADKRAAARLSELGAT
PVPQPGLLAEAAAELAGQYGGAPRVLDMFGGGGTIAFEAALLGCDAHSIDVNELAVFLQQANLTYGATLDHGLLKQLAHRHGQAVLQRLARRTETLYPLR
GRLASQAEKPIVYLWTYRHACPQCGYAYTLSRRPWLSKKAGKALAMVATPGPDGDGVDMVSLPDGEPPPSRWAGRGATACPRCSATATPGIADSADHCVA
VVTSAGVGKQFALRDDALPAAGLLREQEETLLQRTGLALPATTLPAWSGIVNPALYGMRGHADLFNPRQRLVTLMLLEELLAEHRTLQAAHGADVARFVS
ASLSGLIDQMVDWNCRLSMWIPQNEQVGRAFCGPGVAMLWDYAEVDPVGTGPGNLHAKLERICAGIDALALLRRPVQVQRASAAALPYPDDSFDAIVTDP
PYYDNIFYSVLADFFYAWKKPLIDAIENRPTTPPHVPTANSGHDELVASRQRAGGSARAAHDEYCARLGQALREAARVLKPDGLLAFVYSHASVLGWAAL
VTAFRAAPFTINSVQPLSIERKARPRALASEAVNTCTTFVARRAQAPRPVLARAALQAQFDAILRGGLHRSLLRAGWHEHDAGVALIAQGVALLANCAAV
EDADTAALIVDLAGQVSALLPGFSIKQRASL
>M.CviQI [EnzType=Type_II_methyltransferase] [RecSeq=GTAC]
MDKFYTCDEAVDIVLEKVFQFVNITSNDLVIEPSAGDGSFMKKLRYNNVICIDIQPEMSGIVECDFFEYKVPSGFDKIHVIGNPPFGKNGSMALKFMKHS
MKFAESVSFILPKSFKKESFMMKIPKNFELAYQIDLQKNSFLVKGERYDVPCVFQIWKKNNDGKFRSFDIISPNGFSFVKKEEFHHVAFRRVGGKSGKVM
VDTKNCNVNCFYFIRFDPGVTFDIESLQNITFDERDDTAGPRSISKRELIRKYNEIISTWQYDSSCKKL
>BbuB31I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] 
MKTNDIVKTNNPNISLYKQLSKDFIKKENINKLKDFFILIKNKLFSIDDNSTEANIESLLKYIFEELNYSVEQQKAGQIEGVESRVDILLFENDKDKASF
NNKLKEAKKNNEPIPIEDILIIAEVKRPTFSFDAKDKLKESEDQLYRYLNQYQKHYGILSNGKVWRLYDKSKVLYGEKRYIEFNFAKIEEKEEYKEQEWF
VLFIYLIRKERYLKTSNVIEVEKEQIAKEKEIIQKTLKEILYERPDDSIVFKIAKNIYDKEFKLSGKEITQHILASILEESIIFILRIFFIAYIEDNDIF
KKILQENKLYRSSISFRYFFYDENTKKKLEYKKIITIFNLLDKGSDAIKFPVFNGGLFSEDKVKYLNNEGLLSISEIEEILVKMLFFEEKNIKDEKFVKY
SRLDPKSFGELYETLLEYDLRIADTTVHRIIEDGVYLIRTEEELENKKVNKIATYLKGNIYLTSRSLDRKKSGAYYTPDDLTDFMVISSIEEQLKTKSPL
DIKIIDNSCGSGHFLISCLDYLTEKVWYELDKFEDVKKELDEEYRVIIEESEEYDVQDSISKELVLKRMLLKECIYGVDINPISVEITMLSLWINTFIFG
TPLSFIEHHIKAGNALLGYTKDEFFDIVKKKFESGFSLFKKRIKEIITILEDIYQKIKGINDTTKEDIEKSKKIYKEYEESKDIDNLRIIFSLIKLYSLS
FDKSLNMEFSDIASVISLIENILGNKTSSEDKEKIEKIRKLSSYYKFFHYGIEFPDIQEGFDIVIGNPPWEKTKFNETEFFSKHIPNYRKLGIKEQNIIK
QEILSKDNHPLSIEYNEEKNSIIAINNIYKFDFKCFTSGGDPNLFRYFVTFNLKLIKEKGNLTYLVPSAIWNESSSRILRKHIFARYKLNYIYQFENKKR
FKDVHSSFKFAIFQLSNIKESTSSFKAKFMIQSSDNILKEITRDLKDSKDDAYKGIELNINQIKKLSPIQESIIEFKDNEEFTLINKMFSKFSALGEGYI
DFKKGLDPSIKNRKSLLKECNNKNLIFLYSGANIHQFNSRFFEDKDAKESSKLLWIDKEDLEKVLTKDNQYQTERVFYRAIASNTNERTMISTLSPGNCY
CVNSIYINDEKTPISLYKKLFIISIFNSFVFDFLLRRFVDSNVLKSCLYQCPMPQPEEKEILSNPLYLNLAKNTSLLIAKNDPENFKYLLYLEYFKFDKE
KVNKILKLDKEDEFFKEKENENNFIIASLYSLAKEDFITLLGDFKALKNKKKGEDYISSLIKGYDNYLLNNKIFYHK
>BspMI [EnzType=Type_II_restriction_enzyme] [RecSeq=ACCTGC]
MSHDLYAAWAATEITNILQTNPRFLVSDGISRNFTVYASKEGRTKWPIADGVILVEENGRVVYEIAIEFKRRNEGVHGVLTALGQAHAYLHKGYRGSIIV
IPEAYDTHNNPSGHLKEIIEYTSDQVPIGVFSYKDPDVTKTSPFNGKITCIRHLNLNTGLGSVVRSSSPQNFVKTQWAHLREGSSDPDAFFRYLQTSKQL
AIDSLIEPSVNFPPSLVQAIQDIQPGANPLKYLSNSIGNDLHDIVWRNFWFNYILTDEAIPIWNNSEGNYVINDSSTKIVKPDESGNKMFFAGRSDSIKN
RLVNDLNMGNISESEAWKKYALKIRERAHSYREDIDSGLDHIGLLESDGKPSELGYRFVDACERTRNSNSGSPKALLGAAILKNGNLGAFLHYIYRLSEE
KFNADPLAFTKQNNSSGRLQFLHKEYLQWLENELATNLKVMRKVSIRGGASRQPFQGELAILRNYEFVGNFRVGTGLKINWPKIQNAYEVEI
>NgoBVIII [EnzType=Type_II_restriction_enzyme] [RecSeq=GGTGA]
MSNNQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQDQPYSNELYEALQQNIAQDSVMASQRTSKAMNLASVRKAINQFVKMGFIEPFLSSY
TPLSRDYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNKKEIAAMMLVDLKTFQQDYLTETELNDYFQQGIESGFIERK
YNQISYLWNLLDKLDDLKRVGDDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLAYPVLIASHIKPFILSDDTEAYDPN
NGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSDDVWRRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI
>RdeGBI [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CCGCAG]
MQTYYDRTYIQGELSRFTQQWRQKIDSHRRSSRKNFEISETQTFWNSLLACFGIKPGTVAEFERPARRASTGNHGRIDMFWPSVLLVEQKTEGKNLEEAL
DQARDYIAGGSIPEENLPRYLAVSDFERFIFVNRRTGDREEFTLEQLPDRYDTLKFLAGEEAISAEEQQELTIAASKIMANLYQAMMGDTDTPVGEEAAQ
NAEDEDLRVQETSMFLTRILFLLFGDDAGIWEMHLFTRFLRKHTREDGSDLGTQINALFEYLDTPESKRTRMRHVPELMARFPYVNGSLFAERTPTEYFT
RELREQLLIACGFDWSQISTAIFGSMFQLVKSREARRAAGEHYTSEENILKTINPLFLDDLRARAESLIASTKGSKTKRAELAAFLDELGSMHFMDPACG
SGNFLQVAYMRLRELETRVLTEIHRLEDSGGFTLDASLNTRLSISQFHGIEINWWPSKIAEVAMFLSEFQADRTLARALGQVPTRLPITSVAHIVHADAL
TTNWREMIPAEATKVFVFGNPPFLGQWTMSKAQTSAMKTVWGKDYDGYLDFVTAWFWKAVQFFDQDFEGEFAFVSTNSIAQGQPVPALFGPIFREGWRIK
FAYHTFPWDSQAPGQAAVHCVITGFTRSEDYKPRLFEYDWNARQTREAADVKNINAYLLDAPNILVKKRSKPLATQLPPVRFGSKPVDGGNLIVEEKDYA
EISSDSIAAKYLRPFRMGKELVRGLDRWCLWLEDANPADITKSPVLKKRTEAVRTVREKSPKKATQELASVPRLFAEIHQPDTSYVGIPRVVSETRLFYT
VAHLSSDVIAGDKVYTAIDPDGFLFAIISSSMFITWQKAVGGRLKSDLSFSNTIVWNNLPLPEVAEDLRSKIITAGQKILKVRKALAEKHNGRVSLADMY
NPLAMNAELLKAHKELDRHVDKAFGAAKLCASNNKRLEILFARYAELTAE
>M.SspP33I [EnzType=Type_II_methyltransferase] [RecSeq=TTCGAA]
MNNSLKVWEQIVQSYETSDVSLLNEQMRDAYGISNFFKDSEEVRFWKESYKDGELQVGIREFGDFQTPEDLADRVCKVLLKKGISPAVLIEPTFGKGNFI
RAALQNFEEIDQIYGVEIQKQYEIYFKKFILENNPVKFLGKDKLTMEIHQDDFFTHTFPEHLLEGEGEVLVLGNPPWITVAELSTLSSANMPEKSNFKKY
SGLDAITGKSNFDLSEYIITQLLTLLNKTKQGTLAMLCKNSIAKNIVHSLPNQDWNITTLELYNFDAKKEFDVATDASLFLVRLGEKGKGPVNTCDVYSL
SDPDARMKSFGWVNQKFVSDVESYELSAAIDNVSPLVWRSGVKHDCSKIMELTVTEEGKLLNGFKEYADIEETDVYNLLKSSDLKKETIPNKTRKKVIIT
QRKIGQDTLYIKDESPNLWKYLQDNRGYLDKRKSSIYKGKPDFSIFGIGDYSFAPYKVAISGMYKKSNFSLVMPINNKPVMLDDTCYFLSFTKYLDAVFT
LLIVNSPIVQAFLQSLVFIDTQRPYTKDTLMRIDLIEVSSQLTLDDLLDYAKGFDIHIPFGVSEEDYADYKQRLAANPDTFFTGEADSANIQEG
>Mva1269I [EnzType=Type_II_restriction_enzyme] [RecSeq=GAATGC]
MYLNTAVFNIYGDNIVECSRAFHYILEGFKLANISITQEYDLQNITTPKFCIYTDKFRYIFIFIPGTSASRWNKDIYKELVLNNGGPLKEGADAIITRIF
SEDSELVLASMEFSAALPAGNNTWQRSGRAYSLTAANIPYFYIVQLGGKEIKKGKDGKSDKFATRLPNPALSLSFTLNTIKKPAPSLIVYDQAPEADSAI
SDLYSNCYGIDDFSLYLFKLITEENNLHELKNIYNKNVEFLQLRSVDEKGKNFSGKDYKYIFEHKDPYKGLTEVVKERKIPWKKKTATKTFENFPLRNQA
PIFRLIDFLSTKSYGIVSKDSLPLTFIPSEHRVEVANYICNQLYIDKVSDEFVKWIYKKEDLAICIINGFKPGGDDSRPDRGLPPFTKMLTNLDILTLMF
GPAPPTQWDYLDSDPEKLNKTNGLWQSIFAFSDAILVDSSTRDNNKFVYNAYLKEHWVVQREKKESNTPISYFPKSVGEHDVDTSLHILFTYIGKHFESA
CNPPGGDWSGVSLLKNNIEYRWTSMYRVSQDGTKRPDHIYQLVYNSTDTLLLIESKGIKNDLLKSKEANVGIGMINYLKNLMARDYTAVKKDGEWKNIHG
QMTLDKFLTFSAVAYLFTTDFDNEYTSAAELLVHSNTQLAFALEIKEKNSVMHIFTANTVAYNFAEYLLETMRNSHLPLKIYKPI
>PspPI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGNCC]
MKEAVKEKALNILKMDEKVKRLSKAIDDGDTS
>Bbr215II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GAGGAC]
MDDLKSRAARFAESWAGRGDEKSDTQQYWRDLLDKVLLIPNTSDRQVLWFERRTALDGFIDALMIQARVLVEQKSLGVDLDRPEPRQGTMVTPVEQAKRY
SDSLPPSERPSVLITCDFGLFRLYDLEADPLARTPQSEFTLADLPNHISEIGRLFTHENSRVVQQERLSVKAGRRVARLHDSLAECFKNPDDPAEHDALA
MLTVRLVFCLYAEDANLFKPDALRDYVAASTPERLGEDLFDLFEVLDTPIEKRRRYLPESLKAFPYVDGGLFADRIDVPPLTGELRDALLDISEGFDWSG
ISPVIFGSLMEETLSHDERRKGGMHYTSVRNIHRLIDPLFLDGLKSELEQAEAKPVAGGARTNALNRLHDRIAGLRFLDPACGSGNFLTETYLELRRIEN
RILADLDRDGQLALDLGDDLNPVRVSIGHFHGIEINGFACAVARTALWIAEQQALDDTESTITGLPRLPFTDTAHIMQGNALRLDWNTLLPGGQCDYVMG
NPPFIGHVTKTAGQTDDLKTVWGRQYDGYLDYATGWYRKAASYLSKPDAAFAFVTTNSITQGQPVGPLFKPLHADGWHIRFAHRTFAWDAQSTDNAHVHV
IIIGLGRKAKPAPVLFEYADINGEPTARTVDNINGYLIDGPDLYVGKRSQKTGPVSPLLDVTDSGSMPLDGGNLLLADREEYAKAMADPIAARFVRPFRM
GRELINGTDRWCLWLRDAEPGELRKSSFLKKRVDACAEYRRNAPMKGDAYRHRATPWLFRDDHQPSMNYLAIPKVFSEDREYMTCDWYTPDIIAGDMVYT
SPDRDGLAFAVIESRMFMTWQQTIGGRLESRCRFSNTVVWNNLPLPALDDETRTALIEAGRNVLAARANHPGQSLADLYDPDYMPTDLRAAHRELDKVAD
VAFGAGTWLKDDADTRLKVLFDGYARLTGEAS
>M.HpyUM037VIII [EnzType=Type_II_methyltransferase] [RecSeq=TGCA]
MVSNTTLQKNLDAFYTHPKIARFCLDLLKDLTAQNLGLDLNAFHFLEPSAGSGSFVDALKELGIADWDALDIAPKAQGIQKKDYLLELIEFDKKRVIIGN
PPFGHRGKLALDFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKRAKLVLNADLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIAPPKIRHSDFI
TYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALMIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>HpyAIV [EnzType=Type_II_restriction_enzyme] [RecSeq=GANTC]
MDYQTFNEIFNRFVFGTSKAKLLENIAENPERYLGIFRPTKPKTKLLQNLLTSHEIKFGDAFEYLIEQYLKEHNFSPLSKKIPYYNKNKEKRESLELDQL
AKKDNTYYFIEQKMRDDHDSAKKRGQIDNFERKLEALIHRYGENIQGYFYFIDESLNKNQNYYKEELQKLSVGYGVPLRLCYGKELFENLNILQVWDEVL
NHLARWREILPDLPSLNFDENPLESFREIKDLAPSVYRKLLDNDEIFNLVLILFPEQKALKMLAEHFKRQNKTIYQQLASKLEEKLLSLR
>TspARh3I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GRACGAC]
MDGLAARPAHAEESGPAPIAAPEQVEAFLDRWLETGGSERANYQLFLTELCALLDLPQPDPASDDTADNAYVFERRVTIRNPDGSENRGYIDLYRRGCFV
LEAKQTGKGLHTQGWDKAMLAAQNQADQYVRALPEDEGRPPFIVVTDVGRSLELYAEFSRSGGTYVPYPDPSRHRIRLEDLRDPEIQQRLRHLWLAPDQL
DASKHAARVTREVSARLAELAKSLEQQGFDVERVAHFLKRCLFTMFSEDVELLPRGSFTTLLERLQQNPEHFRDAMRSLWETMNTGGYEGQLMHKVQRFN
GGLFQNIDPIPLNADQIGLLIQAGKADWRFVEPAIFGTLLERALDPRERHKLGAHYTPRAYVERLVMPTLIEPLRAEWNTVQVAAEAWLQQNKPDKALDE
LHRFHQRLREVRVLDPACGSGNFLYVALEHLKRLEGEVLNLIRDLSAGQATFDTEGLTVDPHQFLGLEINPRAAAIAEIVLWIGYLQWHYRLHGRLSLPE
PILRDFHNIECRDALIEYDSREPMTDDHGQPVTIWDGISYKKSPVTGELIPDETARTPVYHYSNPRRTEWPEADYIVGNPPFIGEKRMRDALGDGYTEAV
RATYKELPACDFVMYWWHKAAELVSKGAVRRFGFITTNSLVQSRNRGVVDPHLRKSLALLFAIPDHPWVDSVDGAAVRIAMTVGGQAPAPGVLQRVRTEE
ESGDEARTIEFQERRGILFADLTAGAPLVDAQPLASNDGLVFQGVKLVGNGFLVNPEQRHHWVAENPDWAPLLPQLIAGSDLTKRREPRYCIDFFGLDEG
DAKDAFPPGFQKVVDEVRPHREHNKDRFFRENWWLFGRGRGEMRAALRGLSRYIATSEVSKHRYFVLLDRATTLADGSLAVIALEEPHFFGMLSSRLHVR
WALAQGGTLEDRPRWQNGPCFLNFPFPELDDRQAATIGDLAGRIDAHRKARQAEHPDLTLTGMYNVLEKLRAEEPLTAKERTIHEQGLVSLLRELHDDLD
RAVFDAYGWNDLADRIVGRPGATTPLPDKPADQAEAEEELLSRLVALNAERAAEEAQGRIRWLRPDYQAPEATQTTATLDTKPEAAAETPTEPAKKPTWP
KSMPDQVEAVRRLLAIGPQTPEALATHFKRKPTKSITQVLAALQILGQAAYEPHSDHWRLT
>Fsp4HI [EnzType=Type_II_restriction_enzyme] [RecSeq=GCNGC]
MKSFAEIDIERNGNYLKLLSAVSKLSGLFSESAIPFINYRVAENIFCRSFEAGNLSRSDTAFDANYNSMGIGLKTFVCNSNNSTEKVAEFNSLSRVLADF
QGKDLAHKLAEFRNERIDLANRLYNIESSLYHIVARKNNELVLFETDYDKIAIKNIHSVKQNNASLQFEDGKNFYSFNYSKSTLFRKFYIPQNAFKLPIE
ILEDPYSLLLELFEDKTLKPATDKFVRGVNYVILPLYGIQNKQKFVFQKSGLNQWNAGGRKRDFGEVYIPIPIEIHKQFPNFFPARDELFKLKIPTGEFF
SAKVCQDNSKALMTNPNKALSDWLLRKVFQVKEGELLTIEKMNELGFDSVIICKGENGNYKIDKAKLDSYEQFINE
>PacIII [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GTAATC]
MVEWLRRTGQALALVTNGRQWRLVYAGADYDAWCEWDIDLWFNEGQPSAQTIVWRTLLNRNTLLAEQGKSQFLLAISASRKGQAELSSVLGERVRQGVEE
LITASHIAIEQAQQQGQAHDYKDLYVAGSRIIMRCIITLFAEARGLMPVDNPIYQQAYSLEGLRHQLDRRAGGRGNERLSQGRSAWPRLIGLFNLIHAGS
AHERLTVPQYGGALFEPGNPSSEDGISRALALLESHQNLVSDLQVHKLLRLLTRTKMKVRQGRSSRWVDAPVDFSTLSSEYIGILYEGLLDFELKQAAEN
DPVIFLAVGNQPALTLSQLETMEDKDIKQLFEALKKKDKAGDDSDEEGEDDSEEAADIDEGDDSTDEEVESSEDESEENANTSDDSGEGLTISEDHLARA
NAWLQRAAQIAGLVKKPRGRKANTTGDEGQQALEKAGKQLCARMVVPGQFFLVRWGGTRKGAGTFYTRPQLAAPTVRRTLQPLAYEAVRSEIDPRTGLDA
VVEWVPKKPEQILALKVCDPAMGSGSFLAGALRYLTEALTQSLFYHKLIERSEDGGVPRMADGKLTELLSDELTKLNPKDDGFEEALRSQLKRHIVENCL
YGVDLDPLAVELGRMALWVETMDRDLPFGFLDHKLKVGNALVGTWFDTYRDYPAMAWEREGGDKDYQKSKPDNLINHWYEDAKGKRKGDVFTDAIKVRAK
AVPEQLQALLSGQLTLDGGLDAGNVHDELLKVFRKLHRLPVHESEKRAEVYRNEVLNNPHYHALKARMDLWCALWFWPGELIEQAPLPRDFANPSEQVLA
LAADLARRQRFFHWELEFPDVFTATQDGFDVLLGNPPWEIQKPNSKEFFSNHDPLYRAYGKQDALAKQLDYFRALPQLEHDWIAYNANLKGMSNWAKYVG
KPFGDRVTEDKNGKKKHDLNLGSGRNSFESSAAVHRKWLQQRKKHRGFADTAHPFLYQGSADLNTYKLFLEQSHALLRSQGRLGMIVPSGIYTDKGSTDL
RELFLGQCDWQWLFGFENREKVFDIDSRFKFCPLIVQKGGETKAIRAAFMHRNVDDWEHAERYVINVGFDVIKEFSRESFALLEIKEKKELEVLKLLHDR
SRLLRDVVSFTQQFDMTGDSRLFPPISKWCSQGYEQAPELPGFWKSASGNFALTVYEGRMVGQHDFSNKAWISGKGRSAVWNDVEWSSKNIYPQYLIGAE
EYLLSEKRIGGLAFYYMKIGSSTNSRSLVGTLLPDMPSVYSLCQVVSREFDVLDWCVLSGIMNSFVYDYALRFRLGGLNITGYILGETPYPHVCDELRRA
ISFSVACLDFSGLIYSSQWMSLLACGIVRKDRAWRSYWALTTYERCRRLAIVDALVAFSYGLSLEDYLFVLKDCDLPANLLSSSVVRGRLNPKGFWRVDK
DKEPELRHAVLSLVAFHDLQEKGLHAFLAQNDGEGWMIPEQLRLADYGLGHDDRAQEYQPVASRMGPRFYEWQLNEDVERSWQECDAHAELIRGIVPLPQ
SEQADGIAKAQATDKTESPQGALF
>PluTI [EnzType=Type_II_restriction_enzyme] [RecSeq=GGCGCC]
MGEKDTLIEQYLNAAKAWYESQRAQNGSINTNVMNVGLVVSRMIADGFPIDDGRLYSEGRSQVRGLSGNTIAKILEQHGETRLFTREGGRTSRGTIHLAV
SFRDAMNGINRSSGATLNTNHLSLLLEDFFTNCVRIDYFDKQRITVDIETTKPVSSIISDILDAAAERSDKPTGVVLQHLIGAKLQLRFPEIKIGLDRAN
AADMQTDREGDFQVGTTAFHVTTAPMEKLVSRCVENKRAGYRPVILTLESKVLAARQMAENVGMSDQISVQAAEIFIGTNIEEIAIYESDRIRKGVAHLI
RTYNERISNIEIDKSLMIDEPKWIVKLLSRD
>M.HpyFVIII [EnzType=Type_II_methyltransferase] [RecSeq=TCNNGA]
MQTLFKEVTPKRYVNGNEMKENSSNVLDQYFTKPSVALKCFQKACEVIKKYENLDDFIFLEPSAGDGVFYDLFPKNRRIGIDIEPKRDGFIQCDFLNYKL
PTHQKMICLGNPPFGHRGVMALEFINHARNCDFVCFILPMFFESQGKGSIKYRVKGLNLLYSERLEKNAFIDFKNKEVDVHCVFQIWSKKYQNKKSEFSW
YKNRHKEPFGEYIKVFTVSLAKNRECGKEWIFNQKASFYLSSTFYKSTQIVESFEEVKYKSGIAVVFTSANKALNTKLKKLFKEIDWTKYASLATNSCYH
LGKSHIFQALYDHLDILKDN
>M.BdoB079I [EnzType=Type_II_methyltransferase] [RecSeq=TTCGAA]
MNQSLNNFLNSLKGKPLEQANKEAESVCGIVDFFDTEDYSVSEKIEQEKRVEYGDWQTNFELACRVCLLIKNQGITPEVIIEPTCGIGNFILASVIVFGD
TAKDIYGIEIYKPYLNELKFRFLEYALKNPGVINSRIHLIHQSIFGFNFDSLQIDNNKNVLVVGNPPWVTNSKLSEINSENLPKKSNFKNVKGLEAITGK
ANFDIAEYITYQLLDSFHSQNACLAFLIKNSVAKNIVYEQKNGKYNIENMTQYNIDAKKEFNVSVAACLFVAKLGRGKAKQCSVTDLYTLDPPFSYGWVK
ECFVANVDDYMSYSNIDGECQLTWWSGLKHDCSKVMELEKKDGKFYNNLKEEVDIEPDLVYPILKSSDLKGEEVTSFRKYVIVTQKSTSDDTSRIKIKQP
QTYSYLVNHSAWFDKRGSVIYRKRPKFCIFGIGSYSFQQYKIAIAGLYKSTRFSLVKPMDNKCVMLDDTCYLLGFDDEDTANCVLKILNSEIVQRFMKSL
VFYDAKRSINKDLLMRIDLEKAAKKLYTADLLSETEYDIVMSRFERKTELQSLPLFA
>M.Mtu70010I [EnzType=Type_II_methyltransferase] 
MTTFAGKTAASADKVRGGYYTPPTVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGSWDGV
AGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEV
VLFCGVVGPGPAHIRTVRLGDANDLSALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGELADVDVGIVTGRNSFFTFTDAKAQA
LGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADNPTDPTLVAHITAGEAAGVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAPRLTV
NAAAATSTDTVHRVRLDPNVDPATFAAVFHNSATFAFAEIMGRSYGGGILELEPRETEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDG
LGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>M.Hpy57XI [EnzType=Type_II_methyltransferase] [RecSeq=GTAC]
MIRTPKHLNKRESVNLGAYYTPSYLVDCAYKLLKKHVSIEDYTLLDTACGNKEFLKLHHPKKIGADIDPKCDALIINALANPKRENYGINQDEPLIIVGN
PPYNDRTSFIKQDIKNKDFIFEIDNDLKSRDLGISFLRSFAILKPAFICVLHPLSYLIKEANFKQLKLFKDNYRLLDAFVVSSKSFTKSNEFPIVIALYE
RGRMDYADIRRFIFPTDCDTTLCLNDFDYIANYVDKYPNAKKVDACVGYFFPMRDINALKRNKTFLNAPSENAVRISQDKLIYYQYIHYFKEIAPKIPYY
FGNLDIIIENSAFLEIKDAFLKDKRVRLEYFKKLFQGHACEFD
>Mbo26II_(Mbo26IIB) [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=CACGCAG]
MEWQTVTPNLHGDWVNQRDDAFSAWPVIGDKKAALDVTRVFANYSAGLKTSRDAWCYNFSRGALEANIGRTIDFYNSEVDRINEIRGRDAKTPPVDALIT
VDSAKFSWDRINKRQVAQGIRIEFAPAGMRLGTYRPFTKEHAYLDPNQQLNNCTYQLPSMFPTPEHGNVGYYVVGMGSDKPFSCLMLNAIPDLAFWGSSN
GQFFPRWTYEKTEPRDGELDFESTTNAEVDDHGYRRVDNITGVILKLYRDTIGDQVTKDDIFYYVYGLLHDPAYRTKYAADLKKMLPHIPTPETRERFDQ
LASAGRKLADLHVGYESVKPYPLDVQLKPGADPEDRETWRVEKMKWKSKQDHSTIIYNSRVTIAGIPDEAERYLLGSRSALGWIIDRYRVTTDKASGIVN
DPNDWCDEHANPTYIVDLIKKVTTVSVETMKIVDSIVALASAGSDST
>LlaKR2I [EnzType=Type_II_restriction_enzyme] [RecSeq=GATC]
MEKQLFDYDDSDLQSVVDYSQTLLNKKFEQVIEKYQSTPYKTYADFQSKTVSDFKDKEISLKSKGQYGNNIEKYFYGYQPNNDSVADFEKIGVELKVTPF
KVNKNGTISAKERLVLTILKYMEENLDDFYSTHLWQKCSKILLLFYNGLLDGQTIEDYVIKKVFLFEWFEEDMEVILDDYKRITEKIKSGKAHELSESDG
NYLSTCTKGAGKGRDLREQPFSDEPAKQRAWELKSSYMTYLINNKIFNQTDQESVVAVARGEKKSFTEIISERILEFKGMSEAELYEKFSINPKAKGKNS
TLVRTILGLTGDLDKTQEFQKANMNLRVIRVDKNGLPKEDSPFKNYNFKELATNDDWEESHPYQEICNKRFLFVVFKEVKPKIFILDSIKFWGFPDRQVE
EVQRVWAETRRIITEGVELTQHGNRVSTNFPQSRVNKVIFTKIHAQNTYYEIEKNVFVGKGKLSDTDKLPDGRRITKHSFWIPKKFLKEVLEGKWD
>HpyNSH57II [EnzType=Type_II_restriction_enzyme] [RecSeq=TCNNGA]
MDLEQTFLKIIEKKHKELNLGQDYHAIFSKIRDFEANAIGQIGEEFLKSVLNAIDEVINDGIIHDEYDIMTKSGVSFEVKTARKGRTNNTFQFNGINPRY
NYDFLICLGVCEDQLLYRIFKKDEIHYIHKERKYFMKQNEFKKQLVPMNPDNQVNYKLTLNIKELKEITNLIKELERILELD
>CviAI [EnzType=Type_II_restriction_enzyme] [RecSeq=GATC]
MQYNISKITEKAKHTKLPIDGNVIENNIENKIKRIHSKIMKNSDNRVKILKNISVIDWLFGGTMFWKGKSFKTKVEATKFLKKAEDDWGKEMLQKVRPDL
KPSGQWTTKLGEHIAEEIFILLGNIRGEIKKVPVMNGLHPDGEADKFMFEAKSQLYYSTGTAAEKILGVPIKYRNVPDLYKKPLKIVCIAGAELRSEEFG
IFPGTKEYDNKMREILKFYKQNGIEYVRMTDLLSRLDT
>R1.LlaJI [EnzType=Type_II_restriction_enzyme] 
MGKIVLPKHSSDKSDFVTALKIYYEKKGWVENQDFVDDLKREIGDGQYPSSYNKKAQLPKYFGFVITENINGRSPRQKITSLGKTLYEALQNNDQLAIDN
AFMISMENIQFGRNNLGSPSSDSDIEAPNVAMHAIKDLGYITYLELAFILQNLDDNNLDYNSCITEITNSRLSGNFVFDDTNRYSDNKPIMLLVNLGILT
ADEVNSHGRVKSITFSTQFRDSYLNDIPDLKMYNKKEKYIVFEETERSFENESDYDFVERLQVGTNTLLYGVPGAGKSFTIEHEYINEGSPRERLVFHPD
YTYSDFVGQILPVVTEDNDGNKNVSYEFTPGPFTTLLKEAYYNPKVEHFLVIEEINRGNAPAIFGEIFQLLDRKIKKNSSSFPIGTSEYEISNTDIAKFV
YNNPKHKVHIPSNMSIIGTMNTSDQNVFTLDTAFQRRWSMRLIENTFKKDDVELANTKILDTSVTWKKFCETINEIILDKNARMTSSEDKRLGTHFIQIS
DLQPTVESERYNRLFPEKVIKYLWDDAFKFTREEIFDIVNYNSLEKVIRAFIDAEGDKRFSFFVAGVKNLLVSSSSNDDNVSTEE
>TspDTI [EnzType=Type_II_restriction_enzyme] [RecSeq=ATGAA]
MSPSREEVVAHYADRLHQVLQKTIAQNPNEAEFRRAVEPLLEEFLREMGLEPLARAEYTLAQGRADAIFNRLVIEYERPGVLKPRPDAATRHAVQQVKDY
LSGIAQRERHAKERLAGVAFDGRYLIFVRHMGERWVEEPPVEANPHSLKRFLTWLAGLASGIALTSENLNRDFSIEQLRTQTILRGLYQALEKALARKGL
VRQLFEQWRIFFSEAIDYSETFGGRKLEPLKKWVRKAGLDIQTPEEAERFFFVLHTYFALLAKLLAWLALSRHMGVRLGAPVFSALAAADGETLQKRLGE
MESGGIFRQYGILNLLEGDFFAWYLHAWSSEVERALRALIERLDEYDPTTLSLFPEETRDLFKKLYHYLLPREIRHNLGEYYTPDWLAWRLLVQLDNTFF
AGTPSPNDEKLRQKLLSTRFLDPACGSGTFPVLVIGRMLELGRLLMVPERDLLEAILKNVVGFDLNPLAVLTARVNYLLAISDLLQYRQGDITIPIYLAD
SVRTPAEGQDLFSQGIFVFPTAVGDFQVPAALVTAPKRFDRFCEILESSIRSEVDPQAFLERTRRELDLNPSEWDDNARKLAEELYTKLLDLHRRGLNGL
WARLLKNNFAPLTVGRFDYIVGNPPWINWEHLPDEYRESIKHLWLRYRIAGSYTGGRPRLGAVKVDISALMTYIVVDNLLKNGGKLGFVITQSLLKTAAG
AGFRRLSIPTEKGKEVPLRIVYVDDMVDLNPFEGASNRTAVIVLEKGKPTQYPVPYTVWRKNRGVRFTYDSTLEDVQAATRRLQFQAQPVDPDNPTSPWL
TARPKALRAVRKILGQSDYEAHAGVYTGGANAVYWVEPVLKRPDGLWVVRNLTEGAKVKVEEVTETIEPDLLYPLLRGRDVQRWRAEPSAWIIIPQDPNN
PSRAYPEAKLKVDYPRLYAYLKQFESVLRRRAAYQQILCKREPEFYGIMDIGHYSFSPWKVVWTRLAKIEAAVVGLHGRKPVIPQETVSLVQCDTKEEAY
YIAALVNSTAFQLAATSYSQEGGKSMGSMHILEHIRIPRYQPTDPVHRRLAELSQAAHEAAQAGDEKRLEALETEIDREAAKLWGLTEAELREIQESLRE
LEGEVPAAEEEA
>M1.Sgr13350III [EnzType=Type_II_methyltransferase] [RecSeq=GCTCTTC]
MRSDVRRGDRPQVFTRPQRASSGRDHFPKGLTVKRSDLEAAIDDFQAELPYTSGPYASRAWGHKLHSLCSYQGKFKPAMAHWLVKSFSPEGGRVLDPLGG
VGTVPFEAALLGRRAITNDKSPLAATVGAAKMTPPTIDRALEQISRLEKQMLTVDLSAEEIAEAEFGLNASVSAYYHPETLLEVLKARKIFLTKGVQDDA
FMWASLLHVLHGNRPYALSRTSHPITPFNPTGEYLYKSLIERLQNRVTSALAVPLPPEFLEGEAHHGDFRELPDKIADPVDAIITSPPFIGMRFDRPNWL
RMWFCGWSASDFHTKSLEFLERQQTKSTAVYSEFFSTCTQVLAPGGVMAVHIGSGGRGDLVGDLRTLGKAHLDFVGESVENVEKVANHGLKDKGLTKHHH
MLFFQRA
>Cdi1678I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=GCGGAG]
MLLMSSSSPSEKKLAAKLFANKWADRGNEKSDTHSFWLELLRDVVGMEDVTTNVRFESRTSQRGYIDVVIQDAKTFIEQKSIDVSLDKADIRQGRVVTAF
RQALNYANTMPNKLRPDYIITCNFAEFRIHDLNKVNAETDYISFTLAELPDQIHLLDFLIDPQKSRAVREEKVSMDAGTLVGKLYDALRDQYLDPNSDAS
QHSLNVLCVRLVFCLFAEDAGLFEKDAFYRYLDGLRADQVRVALRDLFEVLNTPVDSRDPYLSEQLKNFPYVNGGLFAKDEQIPNFTEEILDLLVHEVSE
KTNWAEISPTIFGGVFESTLNPETRARGGMHYTSPENIHKVIDPLFLDSLKAELDSILNVSGITANKRKKQLEAFHTKISELKFFDPACGSGNFLTETYI
HLRKIENKILSELAGDQTQLGFSNVTLKVSLDQFYGIEINDFAVSVASTALWIAQLQANIEAESIVTANIESLPLRDAAHIHLGNALRTEWASVLAPEQC
NYIIGNPPFLGYSRLDDAQKEDRKAIFGKNGGVLDYVACWHRKAAEYMHGTDAEAALVSTNSICQGQQVTPLWKPLFDAGIHINFAHRTFVWSNEAADQA
HVLCIIVGFSYIDRPVKQAWTYRKNEVEYSEPVHLNGYLAEAPDAFLTRRSKPISDVLEMAQGFKPADGGHLLLTPEERDELLAQEPLAAPWIRKFSMGA
EFINGKDRYCLWLPEITGVELKRLPLVRARIDACREWRLEQIKTGDAYKLADRPHLLRPTSRFKDGTYIGIPKVSSERRKYVPFAFVTDGMIPGDMLYFI
PTNSLFVFGVLMSQFQNAWMRVVAGRLKSDYRYGNTTVYNNFVFPEVDDSVRVDVEKRAQAVIDARSLYPEATLADMYDPDNDFLYPELMKAHRELDRAV
EMAYGVDFGGDEQQIVAHLFKLYNEKVEK
>M.Mbo26III [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGSWDGV
AGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEV
VLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGELADVDVGIVTGRNSFFTFTDAKAQA
LGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAPRLTV
NAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDG
LGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>AhdI [EnzType=Type_II_restriction_enzyme] [RecSeq=GACNNNNNGTC]
MPNPTTAESILHADAQRVIDEINAFTSETFKTRGGRLGSGMGSLIEAMWVYYMNRVLQNEGGEAQLCEIAWLQDHEPADFACLTRGAYWEPESRIGELFR
IEAKSMNLGVDEAKGHFTNLRSETRAHDQLLVLVWSWTTVANNYSWPRIHDYFLGPALPIIELRDALHLQRGGTFVDAQNCPDGCHPLVCSHAGEPLNSA
GKRERRSGPKTCKPANVEYAANFGGLIRMLKTDNDPARNWFRELRRTNPVIHRYISFIHKHYPAEEVNQYRKAEWQTALRYMGLPTNGESASEANSILRQ
YVPNYQDLLRNIIF
>EcoKMcrBC_(EcoKMcrB) [EnzType=Type_IV_methyl-directed_restriction_enzyme] 
MESIQPWIEKFIKQAQQQRSQSTKDYPTSYRNLRVKLSFGYGNFTSIPWFAFLGEGQEASNGIYPVILYYKDFDELVLAYGISDTNEPHAQWQFSSDIPK
TIAEYFQATSGVYPKKYGQSYYACSQKVSQGIDYTRFASMLDNIINDYKLIFNSGKSVIPPMSKTESYCLEDALNDLFIPETTIETILKRLTIKKNIILQ
GPPGVGKTFVARRLAYLLTGEKAPQRVNMVQFHQSYSYEDFIQGYRPNGVGFRRKDGIFYNFCQQAKEQPEKKYIFIIDEINRANLSKVFGEVMMLMEHD
KRGENWSVPLTYSENDEERFYVPENVYIIGLMNTADRSLAVVDYALRRRFSFIDIEPGFDTPQFRNFLLNKKAEPSFVESLCQKMNELNQEISKEATILG
KGFRIGHSYFCCGLEDGTSPDTQWLNEIVMTDIAPLLEEYFFDDPYKQQKWTNKLLGDS
>BssHII [EnzType=Type_II_restriction_enzyme] [RecSeq=GCGCGC]
MGENQESIWANQILDKAQLVMPETHEQNLADTLIDLCYNAAKRTNVPVGIALAASFDLLVGAEYYRNVINRGWCYCPEHQSLIFPYTNTCPACVLSGKFH
FHRSNKPESGKIGTATSRLLCVFLDRLFVKSSRNFKIFKGSEPIDILIHDEQKNIMLLAEVKAAPLITLPLLVRSEEKITDLVDGEIVEIPHSAVDNSSL
SSSNICLLLPVFHDGSWQSKFVELQTKDDILTNTIWAYGQLENIFRGNNDLFDLYLDTWKRAFEAYQVAYHEKDRSSNIFWLTNACGQPEPRPVDWPARS
GTGYESVSDGKTSVGMDRTDDIKKGIYQVLKLGAESKPINQQYQIKTALISNIHAARHYDEYLTSLQDVVWALDETGLAKKAGELDSETPIYNLFDGIIS
FTRNHPRDEWIRENFQF
>M.Mtu26105II [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRD
FASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFL
LSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGEL
ADVDVGIVTGRNSFFTFTDAKAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWW
STPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDV
DLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>Cgl13032II [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=ACGABGG]
MSAFDSILNVEEWISDHYLTNDDAKGASFSKRVRERIKEWKTTEDATQQSGPLTRFSSNRLQLQHALSELDDATTAASLVASALGYGVPSARHAQRGSDT
ISYSSWVGNAGSVEFLAATPAESFEENFRSLPLEPVAVNDKPQDITAAKLVGQIFLSDTPPAFVVITAGKWVVLAERETWPLGRHLAIDISLVVERNDTK
AQGEMQQTVVALARENTERAADGTTWWEETIEQSREHAVKVSGELRSAVRESIEILGNDVLTRYEAKELSTAEIDGGELAKQSLRYLYRILFLLFAEASP
ELEILPTGTPEYDEGYGLARLRELILNPPVTQHARTGTHLYDSLQLLFTLVDKGHHPTDAKAVAFDAEAGEEGLHFRNLSADLFLPAATELIDRVGLSNE
ALNKVLENLLLSRVQSGKDRGFISYATLGVTELGQVYEGLMSYTGFIAQEDLFEVAPHGKADKGSWMLPVSKADEVPADSFIEVDQEAPGGGVIKVRKRH
PRGSFVFRQSSRDRERSASFYTPQVLTSFTVTQAIEELQASKRITTANDVLSLTICEPAMGSGAFAVEAVRQLAELYLELRQEELEQQIPAEDRAKELQK
VKAHIALHQVYGVDLNSTAVELAEISLWLDTMNAEMDAPWYGLHLRNGNSLVGATRSLYAPSLLNKKAWLTATPTRYRLDDIAQAIDENKAEPLFNHGIH
HFLLPSTGWGATADAKDLKDLMATEIKELKSWRTSIRASLSKTQIKQLNNLALRVETLWRFVLMRIRIAESQISRSTTLWGQEPAEVSEVVTREQIEQDL
FGNIDGAYNRLRLVMDAWCALWFWPLDAVATAEHPERPALPDLDEWLATLTEILGIDLPLKSKNENQIVLGPDTNWLAINDAEATDLGFSGALSFERVSA
NHPWINVARQVAKQQSFFHWDLDFAHVFAKGGFDLQVGNPPWVRPDVNFEDLLAEHDPWWAVMSKPTQASKKERQKNFHNNPKSLEHVVSGAGETVATSA
ILGSVTLYPHLKDQRPDLYRGFMEKTWSNASPAGAISLIHPESHFTEKKAAPLRRGAYERLRRHWQFINELILFDVHDLVKYGVHVYGAPQESINFLSAA
SLYHPQTVLDSFDHDGSGNLPGLKDDNGNWDRRPHKDRIQLVNADTLTVWKSILEDEQTPYLDTRMVYTVNTEAAAVLEKLASAPRIKELGLQFSSGWNE
TTDKKKGYFDVGWGYPASWSDAILQGPHLGVATPMIKQPNPTMKHNQDWSEIDFEAIPANFIPATAYQPDRQTKPTYDADYGTWTFGDKQVPVADTFRIA
WREMAATTGFRTVYPSVIPPGANHVHTVNSAASRSNLKTILVGAQLGAILSDYFARSSGSSHIFNDIVRKIPLPNFTSLEKQFARTYLRLNCLTSAYAPL
WEEITGEPWDVQVPLRNAEQRRAAQNDIDAMVALSLGISADELCMIYRTQFPVMRRYDQEDHFDANGRKVPKEIIKLQQKLKDGQELSVEKRTWVHPQSE
VSYTFEYPFRVLDREADLRAAYAKFENQLKEP
>M.PfrJS9aIV [EnzType=Type_II_methyltransferase] [RecSeq=ARCCCC]
MTTSRPRVLIEDWLPVAELGIESRRERGAASALPPLSFLHIWWARRPLVASAAVALAGVMPAWTEELAESFPDAPEVRTESAYRGWLLRLVGIWGDPISA
RRIYDAAVASGVRVANPYTYRQAFRNAIPRSDIDLLHALLRRTWGTLPVVADPTAGGGSIPWAASRLGLPVVANDLNGVAASVLKAGVEIPATRGLDLLP
DIGKWGDVLVKRVEKRLKEFFPSGEGEVVATYLFANAVPCPRTGRLVPLLTDKWLRKTAGKEAAVRMVTSVGGIELREPRFEVILGREVDKADASTGTMA
RGKAVSPYDNLVIDGDYIKEAAQSGDMSQLLYAVAVRKPSGERTFRAPNEADLQALQAANRTFDAVKDEWFDAGILPTEEFPEGNDLRPKHYGLDRWVDF
FTPRQALVHGTFGEEFARLVPEVREALGEAADQVLFELALMQGKALNYNSRLGSWHVSKQVMRSVFERHDFAFKWTFAEFEGATALYSWCLDQLTDAYGG
IARLLDETGSAELGTNDRLERQVTVTQGSAASLTLDDGSVTHICMDPPYYNNVMYAELADFFYVWEKRTLGRLVPDYFRDELTDKDNEAVANPARFAAMG
RRKNELADLDYESKMTSIFAESRRVLADDGVLSVMFTHKRAEAWDTLGMGLLQAGFTIETSWPVNTEAETSLHQANMNSAASTIMLVCRKREDRETEHRT
YLDDIEQDIRLAARDAATRFQHDGIDGVDLLLSTYGPTLSVISQNWPVYSSAPDADGRDQLLRPEDALALAREEIVDLRRSRLVGQAAKVDGLTDFVLLA
WDTFGAREFPFDTARLLALAVGGLDVDELERAKIVSKASGKVKLLTPKERLRREADSGLPGVTPEASSFEFVIDAVDTALYIAEVDGQQAAKRFLDRHGY
TSDAGFISTLQGLANAIPRTKVKGAWVLPEAGLIDTLCTLYFDDVVLPEAEEMAAPTDPDENTLFDVE
>M.HpyNSH57I [EnzType=Type_II_methyltransferase] [RecSeq=GTAC]
VNLGAYYTPPYLVDYAYKLLKKHVGIENYTLLDTACGNKEFLKLHHPKKIGADIDPNCDALIINALANPKRENYGISQDEPLIIVGNPPYNDRTSFAKQK
LKDKNFHFEIDNDLKSRDLGISFLKSFAILKPAFICVLHPLSYLIKESNFKQLKLFKDHYRLLDALVVSSKSFTKSNEFPIVIALYERGRMDYAGIRRFI
FPTDCDTTLCLNDFDYIANYVDKYPNAKKIGACVGYFFPMRDINALKCNKTFLNAPSANAVYISQDKLIYYQYIHYFKEIAPKIPYYFGNLDIIIDHFAF
LEIKDAFLKDKRARLEYFKKLFQGHPCEFD
>M.PhoIII [EnzType=Type_II_methyltransferase] [RecSeq=GCNAGA]
MEKRLIESPNFPIEEVNEKSLKEKGPGRPPYWEMVFWWTRKPLIGARAIIAASLLPEDYDVNKFKSKIRLSEGHKANPEPMFRGKKLLDPFAGFGSIPLE
GLRLGLDVTAVELLPTAYIFLKAVLEYPKKFGRQLIRDVEKWGNWITEQLKNDPEIKELYDEDVAVYIGTWEVKCPHCGRWTPAVGNFWLARVKDGKNYK
RLAYMKIDPNGEVRIIDLNEIFADVSGAKVSTERGEIILEGKGYVEKVKKAIERGKLKEEDVEIEGDKVVFKVPRPNIEGRKSQLVCLSCGNVIKYADEE
GNHYIEKPKGKNVDFYVKFALRKYHEGNERFARQRLLVKVKVKNKDLIFEPATREDNEKLWKAKEKVKEMVEKKDPDVPTEKMQPYGGATLGDIYKVFIS
WNNLFNPRQLLTLIKIVRLIREVGKKVEEEKIAEGWSKERAFEYAEAVATYLSIVLVRLMNFNSICTSWYSGSLLTNKIQSSLSFRGIAMVWNWGDTNIL
YEEGNSYSFRKSTESVLRALQYLTSALSSSQKTLADFTGNSIKVLQGDATSLNLGEKFDVIVTDPPYADDVPYTELSDFYYVWLKRALSDVENGKLVPRF
HREAFFKRIGPKWVEIKTQWQEFAKKEVSMYPARFDGKSGKEAKEIASKHFENLFSQAFVAMREHLKDDGILVTYYAHTDLESWITLIEAGWRKAKLQVT
RAFPLGTESSQRVTARGKMALDTSVVVVWRKRNEEKEVWVGDIEETLTEVGMKSAKEFISHGYHRLNLLYGVMASVLSEVTKYKAVKSPKGELSVREILE
KYVYPATIRGIVGAITEDVKAETLSREGIFYTAYKVLFGNAKVSPNDIILLNLATAVDSSSLQKLRILKRVNSEITLYSPDLLGKKALDARELQKFLKER
GAEKYRNSIDVLHVLEHASLLGASKVRETIEELKSKMEVNEAISIAKLVASYYKRIYSLTLGIKDEKEITKRLEKDGLYEVILMERLVKAVRGEML
>BbrUII [EnzType=Type_II_restriction_enzyme] [RecSeq=GTCGAC]
MSDEYTLTVDMATVDSLGRNLYSNAAAVLSEFVANAWDADANNVSIDYKPDEGSGSITITDDGCGMDTDTLNSRFLTVGYQKRKREGDESFRFHRKYMGR
KGIGKLSAFSLADIMQVESKREGAEARGFTIDVARLDEAIKSSDPNERQYHPAPISRDGLTLNDCEHGTKIVLTSLRRKRIGLTLEALRRRIARRFDVLD
LADKSEEEGAFRIVINGKPVTYEDRGDLQRLEHIWYLGNFSPSKEQQNSIKAEHHRVEDVSFDGHPGWKLSGWIGTVRRPSDRAFSDDTESMKNIIVMAR
KRPIQEGLLDHLDFNRNFASYVTGQVQADFLDDSQQDDIATSDRQRLVESDERVKAFNEKMKDIFNRASDEWSRARSDANTKKLMADIPEVAAWVDSLTE
GLRKPARDLIDRVSRINGIPEADRRSLYQSSIMAFTRLVQRDDIEKLYRVGELSTEQLLAILTSYKDYENMEYAQVVRTRLEVIDKLESMLKANELENAT
RDFVSDNPWLLDPAWERATRNAEKEKAFRTIGEEEYKLDFSSDSESNSRVDIKYLTAPDRETIVEFKRYGRDIKIEEIREQVWRYAEAMTRILEKDDART
DRAEGRKNASGIDERVNVLIIVRRVLDNRGKPMSPGAANAQVEMFRAKFLYFSDMLAQSRERYQEFVKGVSQKDYAGEAIQAIESSGSAGNAENI
>M.MtuJKIII [EnzType=Type_II_methyltransferase] [RecSeq=CTGGAG]
MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRD
FASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFL
LSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHIRTVRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGEL
ADVDVGIVTGRNSFFTFTDAKAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWW
STPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAEQLPMPPPAYGSAELAQDV
DLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>SonI [EnzType=Type_II_restriction_enzyme] [RecSeq=ATCGAT]
MNSLMTGLIKVDNITRSGLTIYDRIRTGDQQFWLTSEELSAVLNLRLKGTSFYGLAIRTRSKVAKELVCNALGYKVPSSFVKCQPRFSGQMFDTYVQKSN
NLQVWNEELDVERRYVIIRTDENDIITQVKVIAGSDLAVLDTTGTLTQKFQARLTLSDQKMELISHFDTLNIQKILAPISLNLEIDSPVSQPQNGKLLPI
GECFRRLSKIVGCSFTDAGRTQERNRGAALHALVCKALGYTDYRDNGQFPDIKHQLLEVKLQTSPTIDLGLVCPNSQANLDIEQLGLQQIRHCDVRYAIF
YGYIVNGLVHITNLYLTTGEDFFNRFEQFGGKVLNKKIQIPLPRDFFS
>Lpl520I [EnzType=Type_IIG_restriction_enzyme/methyltransferase] [RecSeq=AGCCAG]
MKEADQKRAATAFVKRWQDRGNERQDSQTFWLDLLETVYDIPNPGEYISFEDRVMLDHTSFIDGFIDTTKVLIEQKGRNKSLFDGIRQSDGSLLSPFEQA
KRYSANLPYSKRPRWIITSNFTQFFVYDMEQPNGEPAVIQLKDLPTEYYRLGFIVDQLNPHLDKEMEVSMQAGELVGKIYDELFTQYRDPTNASSQRSIN
ELSVRLVFCLYAEDAGIFGRHGMFHDYLNEFETRHLRTALIDLFRVLDTPIDKRDPYLEEKLAEFPYVNGGMFTNEEVEIPQFTDSLRALILDQASKEFD
WSEISPTIFGAVFESTLNPDTRREGGMHYTSIANIHKVIDPMFLDGLRNELTEIKQIKQRKTMIQRATDFQARLGKLRFFDPACGSGNFLTETYLSLRKL
ENQVISLIYGNESMLAVDDDLIKVSIQQFYGIEINDFAVSVGKTALWIAESQMMEETAAIVYANMDFLPLKTYTNITEGNAIRLDWETVVGGERITYIIG
NPPFYGHEKRTRAQAADMDIAFHDFTKYGKLDYVAAWYNKAADFIQGKTTEVAFVSTNSITQGEQAPTLWPKLFEKGIEIQFAYRTFRWSSEAKEKAQVH
VVIIGFTGYPRKEKKRLFEGGQMKLVDHINAYLTAGTDVFPQPRRKTSISGANFPVMSKGSQPTDGGNLILSPAERAALIKQYPQTESYIRRYVGSRDFI
NNVERYCIWLKGVSPSQFRSVPPIMERLAAVTEARLKSPTPSVKALAEMPMLFTEIRQPDSNYLAVPEVSSENRRYVPMGWMSPAVIASNKLYLVPNASL
YMFGVMTSNVHMAWMRAVAGRMKSDYSYSPTVYANFPWPEVDDKQKAQIEQTAQGILDARKLYPDSSLADLYDPLATVPELRKAHQDNDRAVMRAYGLTI
KGTTESDTVAMLFERYRQLTK
>M.HpyUM037XI [EnzType=Type_II_methyltransferase] [RecSeq=GTAC]
MNLGAYYTPPYLVDYAYKLLKKHVGIEDYTLLDTACGNKEFLKLHHPKKIGADIDPKCDALIINALVNPKRENYGISQDEPLIIVGNPPYNDRTSFAKQK
LKDKNFHFEIDHHLKSRDLGISFLKSFAILKPAFICVLHPLSYLIKEANFKQLKLFKDNYRLLDAFVVSSKSFTKSNEFPIVIALYERGRMDYADIRRFI
FPTDCGTTLCLNDFDYIANYVDKYPNAKKVGACVGYFFPMRDINALKRNKTFLNAPSANAVRISQDKLIYYQYIHYFKEIAPKIPYYFGNLDIIIDHFAF
LEVKDAFLEDKRARLEYFKKLFQGHPCEFD
>S.SglORF2102a [EnzType=Type_I_specificity_subunit] 
MNNLVLIPKYETYQISGVEWLGDILGSWNLLTNKYIFKLKKILVGKKSDEYELLSLTLRGIIKRDMDNPESKLPAEFNTYQKVKRGNFFSLMSKRSQEQL
VYLYNGMITGTIHYSKISTEFNSKFLYYFYLNLDLNKRLKPLYRGLRNKIPKEFFMGFKTFIPPYEQQYLIACYLNKKTAQIDEAIDIKEQEINLLKERK
QNIIQKTVTQGLDPNVPLKDSGVDWIGKIPEIRKIRRSKFLFTQRKERAWKDDVQLSATQAYGVIPQEQYEELTGKRVVKIQFHLEKRKHVEKDNFVISM
RSFQGGLERAWSQGCIRSSYVVLRALEDIDTAFYGYLLIITILHRCLTTNYKLYSRWAGFELR
>S.PveR02I [EnzType=Type_I_specificity_subunit] [RecSeq=CAANNNNNTCGG]
MESIVRVPVADFSPERLDTGFYSKEFYSARELIENAGFELEAIGSVCEPWQFGAYALCNDIVWDGAETGVPFIKAEAIESPLIDEASLSYVTPKTHSILS
KSALKAGDIIVSTSGTVGRLAVLPDSIPVANSNQDTIKFSLAGTNYNSHFVAAWLTTKYGQAFMSREAGGAVQQHIYLYNFKRLPLLKLHNGAQNYIGEK
IHQAERLKAWARRIIAGQNAIYSEQYAALSDYKKYKSQFFRMSFKTAFDVLTPESYPPEIDQYFRENEFVTLGEISSEIFTGNTREESENSSEAVAQATS
RSCTGLFLKGPFNKVERPSKGKYLKKFDILLTCGAHDKKYIGRDVSIYHADDENLPSSKVLVVRVKEGSLPASYVHSYLTSDAGYIQWQSVVRGVSAGIH
PGDVSRIRIPVPLKIRGWDSIAHIADDQFSSAGKAVDFAQKLTRAARFLVEAVIEGRLDESLLKAAQVLLEGGNESVDRSILNRLKTDGIDGGGQQLFSD
LDELYRLLDQAQED
>Csp68KIII [EnzType=Type_II_restriction_enzyme] [RecSeq=ATGCAT]
MIKKHQFTQIILEILTQYFDENAEDIFQNSPLLQYLNIKTKSANKGSKSRPSLGNHYALYVLVEDYINKGFYNQNNYEEYEGARFSDLLRRQRELPFGEK
LQNHALNHRLNMEFSKYFPTIEQKPILRDVETSRYWINENLLIIEVSKIKYNIALVVKHIIDAYVNARQQSFRDFMSYCDELLQIENKDNNDAVNFIKTL
LRPNIDARVFEITSYAILKTFYAEQKIFWGYSLEELTEDNLILYKTGRTNANDGGIDFVMKPLGRFFQVTETIDVNKYFLDIDKVQKYPITFVVKSNETT
EKIKEKIQQQAKLKYKINAIVNRYLDSVEEIINLPILHEYINTIIEQGNISDIIQEIAVQSQLEFNLNSSNNN
