This DATSETNAMEreadme.txt file was generated on [2022-08-15] by [Rebecca Prosser and Debalina Acharyya] ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Ex vivo Comparative Investigation of Suprachiasmatic Nucleus Excitotoxic Resiliency 2. Author Information Principal Investigator Contact Information Name: Rebecca Prosser Institution: UTK Address:Department of Biochemistry & Cellular and Molecular Biology, M309 Mossman Building, Knoxville TN 37996 Email:rprosser@utk.edu Associate or Co-investigator Contact Information Name:Debalina Acharyya Institution: UTK Address:Department of Biochemistry & Cellular and Molecular Biology, M309 Mossman Building, Knoxville TN 37996 Email:dacharyy@vols.utk.edu Alternate Contact Information Name:Joanna Cooper Institution:Univ. Maryland School of Medicine Address:The Center for Vascular and Inflammatory Diseases, Univ. of Maryland School of Medicine, Baltimore, MD 21201 Email:JoCooper@som.umaryland.edu 3. Date of data collection (single date, range, approximate date) 2019-2022 4. Geographic location of data collection (where was data collected?): UTK 5. Information about funding sources that supported the collection of the data: None 6. Abstract/description of the dataset: Histology images and excel files with the data generated by analyzing the images associated with the paper "Ex vivo Comparative Investigation of Suprachiasmatic Nucleus Excitotoxic Resiliency" 7: Keywords for the dataset (provide 3 - 5): suprachiasmatic, excitotoxicity, NMDA, circadian, brain slices -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: All files are CC0 -- public domain 2. Links to publications that cite or use the data: Once the manuscript is published, we will have one. Currently in a dropbox: https://www.dropbox.com/home/Excitotoxicity%20Manuscript%20data%20files%20for%20open%20access 3. Links to other publicly accessible locations of the data: 4. Links/relationships to ancillary data sets: 5. Was data derived from another source? If yes, list source(s): 6. Recommended citation for the data: Ex vivo Comparative Investigation of Suprachiasmatic Nucleus Excitotoxic Resiliency --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: 200.tif; 75.tif; 78.tif; 58.tif; 199.tif; 198.tif; 200_ROI.jpg; 75_ROI.jpg; 78_ROI.jpg; 58_ROI.jpg; 199_ROI.jpg; 198_ROI.jpg Short description: control SCN images and ROIs associated with Figures 2, 3, 5A, and 6A B. Filename: 200.tif; 75.tif; 78.tif; 58.tif; 199.tif; 198.tif; 200_ROI.jpg; 75_ROI.jpg; 78_ROI.jpg; 58_ROI.jpg; 199_ROI.jpg; 198_ROI.jpg Short description: control AH images and ROIs associated with Figures 2, 3, 5B and 6B C. Filename: 154.tif; 155.tif; 156.tif; 157.tif; 202.tif; 203.tif; 154_ROI.jpg; 155_ROI.jpg; 156_ROI.jpg; 157_ROI.jpg; 202_ROI.jpg; 203_ROI.jpg Short description:control cortex images and ROIs associated with Figures 2, 3, 5C and 6C D. Filename: Figure 2A.xlsx; Figure 2B.xlsx; Figure 2C.xlsx Short description: raw image analysis data for the graphs A, B, and C of Figure 2 E. Filename: 63.tif; 65.tif; 67.tif; 215.tif; 216.tif; 217.tif; 63_ROI.jpg; 65_ROI.jpg; 67_ROI.jpg; 215_ROI.jpg; 216_ROI.jpg; 217_ROI.jpg Short description: 50uM NMDA SCN images for ZT6 and ROIs associated with Figure 3 , 5A and 6A F. Filename: 63.tif; 65.tif; 67.tif; Image 6.tif; Image17.tif; 215.tif; 63_AH.jpg; 65_AH.jpg; 67_AH.jpg; Image6_ROI.jpg; Image 17_ROI.jpg; 215_ROI.jpg Short description: 50uM NMDA AH images for ZT6 and ROIs associated with Figure 3 , 5B and 6B G. Filename: 158.tif; 159.tif; 160.tif; 161.tif; 228.tif; 229.tif; 158_ROI.jpg; 159_ROI.jpg; 160_ROI.jpg; 161_ROI.jpg; 228_ROI.jpg; 229_ROI.jpg Short description: 50uM NMDA cortex images for ZT6 and ROIs associated with Figure 3 , 5C, and 6C H. Filename: Figure 3A.xlsx; Figure 3B.xlsx; Figure 3C.xlsx Short description: raw image analysis data for the graphs A, B, and C of Figure 3 I. Filename: 70.tif, 70_ROI.jpg, 79.tif, 79_ROI.jpg, 81.tif, 81_ROI.jpg Short description: 100uM NMDA SCN and AH images and ROIs associated for Figure 5A and 5B J. Filename: 72.tif, 72_ROI.jpg, 74.tif, 74_ROI.jpg, 84.tif, 84_ROI.jpg Short description: 500uM NMDA SCN and AH images and ROIs associated for Figure 5A and 5B K. Filename: 310.tif, 310_ROI.jpg, 311.tif, 311_ROI.jpg, 312.tif, 312_ROI.jpg, 313.tif, 313_ROI.jpg Short description: 1mM NMDA SCN and AH images and associated ROIs for Figure 5A and 5B L. Filename: 304.tif, 304_ROI.jpg, 305.tif, 305_ROI.jpg, 306.tif, 306_ROI.jpg Short description: 10mM NMDA SCN and AH images and associated ROIs for Figure 5A and 5B M. Filename: 318.tif, 318_ROI.jpg, 319.tif, 319_ROI.jpg, 320.tif, 320_ROI.jpg Short description: NMDA+AMPA SCN and AH images and associated ROIs for Figure 5A and 5B N. Filename: 307.tif, 307_ROI.jpg, 308.tif, 308_ROI.jpg, 309.tif, 309_ROI.jpg Short description: 1mM NMDA Cortex images and associated ROIs for Figure 5C O. Filename: 314.tif, 314_ROI.jpg, 315.tif, 315_ROI.jpg, 316.tif, 316_ROI.jpg, 317.tif, 317_ROI.jpg Short description: 10mM NMDA Cortex and associated ROIs for Figure 5C P. Filename: 312.tif, 312_ROI.jpg, 322.tif, 322_ROI.jpg, 323.tif, 323_ROI.jpg, 324.tif, 324_ROI.jpg Short description: NMDA+AMPA Cortex and associated ROIs for Figure 5C Q. Filename: Figure 5A.xlsx, Figure 5B.xlsx, Figure 5C.xlsx Short description: raw image analysis data for the graphs A , B and C for Figure 5 R. Filename: 85.tif, 85_ROI.jpg, 87.tif, 87_ROI.jpg, 88.tif, 88_ROI.jpg, 269.tif, 269_ROI, 270.tif, 270_ROI.jpg, 271.tif, 271_ROI.jpg Short description: Control SCN and AH for ZT12 images and associated ROIs for Figure 6A and 6B S. Filename:86.tif, 86_ROI.jpg, 89.tif, 89_ROI.jpg, 90.tif, 90_ROI.jpg, 255.tif, 255_ROI.jpg, 256.tif, 256_ROI.jpg, 257.tif, 257_ROI.jpg Short description: 50uM NMDA SCN and AH for ZT12 images and associated ROIs for Figure 6A and 6B T. Filename: 44.tif, 44_ROI.jpg, 46.tif, 46_ROI.jpg, 104.tif, 104_ROI.jpg, 105.tif, 105_ROI.jpg, 106.tif, 106_ROI.jpg, 242.tif, 242_ROI.jpg Short description: Control SCN and AH for ZT16 images and associated ROIs for Figure 6A and 6B U. Filename: 43.tif, 43_ROI, jpg, 101.tif, 101_ROI.jpg, 102.tif, 102_ROI.jpg, 103.tif, 103_ROI.jpg, 243.tif, 243_ROI.jpg, 244.tif, 244_ROI.jpg Short description: 50uM NMDA SCN and AH for ZT16 images and associated ROIs for Figure 6A and 6B V. Filename: 184.tif, 184_ROI.jpg, 185.tif, 185_ROI.jpg, 186.tif, 186_ROI.jpg, 291.tif, 291_ROI.jpg, 292.tif, 292_ROI.jpg, 303.tif, 303_ROI.jpg Short description: Control SCN and AH for ZT22 images and associated ROIs for Figure 6A and 6B W. Filename: 181.tif, 181_ROI.jpg, 182.tif, 182_ROI.jpg, 183.tif, 183_ROI.tif, 279.tif, 279_ROI.jpg, 280.tif, 280_ROI.jpg, 281.tif, 281_ROI.jpg Short description: 50uM NMDA SCN and AH for ZT22 images and associated ROIs for Figure 6A and 6B X. Filename: 94.tif, 94_ROI.jpg, 95.tif, 95_ROI.jpg, 96.tif, 96_ROI.jpg, 276.tif, 276_ROI.jpg, 277.tif, 277_ROI.jpg, 278.tif, 278_ROI.jpg Short description: Control Cortex images for ZT12 and associated ROIs for Figure 6C Y. Filename:266.tif, 266_ROI.jpg, 267.tif, 267_ROI.jpg, 268.tif, 268_ROI.jpg, Image2.tif, Image2_ROI, jpg, Image4.tif, Image4_ROI.jpg, Image6.tif, Image6_ROI.jpg Short description: 50uM NMDA Cortex images for ZT12 and associated ROIs for Figure 6C Z. Filename: 111.tif, 111_ROI.jpg, 112.tif, 112_ROI.jpg, 113.tif, 113_ROI.jpg, 231.tif, 231_ROI.jpg, 234.tif, 234_ROI.tif, Image9.tif, Image9_ROI.jpg Short description: Control Cortex images for ZT16 and associated ROIs for Figure 6C Aa. Filename: 108.tif, 108_ROI.jpg, 109.tf, 109_ROI.jpg, 252.tif, 252_ROI.jpg, 254.tif, 254_ROI.jpg, Image12.tif, Image12_ROI.jpg, Image16.tif, Image16_ROI.jpg Short description: 50uM NMDA Cortex images for ZT16 and associated ROIs for Figure 6C Ab. Filename: 133.tif, 133_ROI.jpg, 134.tif, 134_ROI.jpg, 135.tif, 135_ROI.jpg, Image18.tif, Image18_ROI.jpg, Image20.tif, Image20_ROI.jpg, Image23.tif, Image23_ROI.jpg Short description: Control Cortex images for ZT22 and associated ROIs for Figure 6C Ac. Filename:128.tif, 128_ROI.jpg, 129.tif, 129_ROI.jpg, 130.tif, 130_ROI.jpg, 131.tif, 131_ROI.jpg, 132.tif, 132_ROI.jpg, 286.tif, 286_ROI.jpg, 287.tif, 287_ROI.jpg, 288.tif, 288_ROI.jpg Short description: 50uM NMDA Cortex images for ZT22 and associated ROIs for Figure 6C Ad. Filename:Figure 6A.xlsx, Figure 6B.xlsx, Figure 6C.xlsx Short description: raw image analysis data for the graphs A , B and C for Figure 6 Ae. Filename: Annotation for figures.docx Short description: Description of how the image files are organized and what they contain 2. Relationship between files: The data in the excel files are based on the tif file images, and the ROI files indicate what areas of the tif files were analyzed. 3. Additional related data collected that was not included in the current data package: 4. Are there multiple versions of the dataset? no If yes, list versions: Name of file that was updated: i. Why was the file updated? ii. When was the file updated? Name of file that was updated: i. Why was the file updated? ii. When was the file updated? -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: 2. Methods for processing the data: 3. Instrument- or software-specific information needed to interpret the data: 4. Standards and calibration information, if appropriate: 5. Environmental/experimental conditions: 6. Describe any quality-assurance procedures performed on the data: 7. People involved with sample collection, processing, analysis and/or submission: ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: [FILENAME] ----------------------------------------- 1. Number of variables: 2. Number of cases/rows: 3. Variable List A. Name: [variable name] Description: [description of the variable] Value labels if appropriate B. Name: [variable name] Description: [description of the variable] Value labels if appropriate 4. Missing data codes: Code/symbol Definition Code/symbol Definition 5. Specialized formats of other abbreviations used