Date of Award
Master of Science
Ecology and Evolutionary Biology
Randall L. Small
Edward E. Schilling, Benjamin M. Fitzpatrick
Studying plant-pollinator relationships essential for understanding angiosperm evolution. In the large endemic genus Penstemon (Plantaginaceae), shifts in pollination syndrome are proposed to be important for explaining taxonomic and morphological diversity (Wilson et al., 2004; Wolfe et al., 2006). However, little work has been done to determine the relationship between morphological and genetic divergence within pollination syndromes. This study utilized genetic data to explore whether divergence in corolla morphology among nine closely related, bee pollinated Penstemon species was consistent with pollinator-driven selection.
Bee pollinated species in Penstemon subsection Penstemon are often divided into two morphological groups based on inflation of the corolla throat. This trait has been proposed to be an important target for pollinator selection (Pennell, 1935; Clements, 1995). Consistent with this theory, phylogenetic analyses of the nuclear gene GBSSI indicated that genetic divergence among species was consistent with morphological divergence. However, a similar pattern was reflected by chloroplast data (noncoding region rps16-trnK), indicating divergence likely occurred during a period of geographic isolation. Therefore, pollinator-driven selection on corolla morphology does not appear to be the primary cause of divergence among morphological groups. Instead, gene flow patterns indicate that pollinator selection may serve to reinforce pre-existing divergence between groups.
Population genetic analyses utilized microsatellites in addition to GBSSI and rps16-trnK and focused specifically on two species that share a high degree of morphological similarity. Penstemon hirsutus and P. tenuiflorus have small average population sizes, fragmented geographic distributions and are pollinated by insects (primarily bees) with short foraging ranges. Patterns consistent with geographic isolation of populations were observed in P. hirsutus, but P. tenuiflorus nuclear data reflected much lower levels of genetic structure. Although P. hirsutus and P. tenuiflorus are highly similar, these findings imply that pollinator dynamics differ between species. Therefore, a trait other than corolla throat inflation may be important for explaining differences in pollinator-driven selection. Furthermore, P. tenuiflorus may represent an example of Slatkin’s paradox. Higher than expected pollen-mediated gene flow among populations of P. tenuiflorus suggest that long-distance foragers such as moths may be more important than previously realized.
Becker, Anna Katherine, "Genetic variation and dispersal in Penstemon hirsutus and P. tenuiflorus. " Master's Thesis, University of Tennessee, 2013.